LC–MS/MS analysis of steroids in the clinical laboratory

Salivary cortisol as a biomarker in stress research

Hellhammer, D. H., Wüst, S., & Kudielka, B. M. (2009) · Psychoneuroendocrinology · doi:10.1016/j.psyneuen.2008.10.026 · Cited by 1,925 · see citing works

The fundamental flaws of immunoassays and potential solutions using tandem mass spectrometry

Hoofnagle, A. N., & Wener, M. H. (2009) · Journal of Immunological Methods · doi:10.1016/j.jim.2009.06.003 · Cited by 562 · see citing works

Urine steroid metabolomics as a biomarker tool for detecting malignancy in adrenal tumors

Arlt, W., Biehl, M., Taylor, A. E., Hahner, S., Libé, R., Hughes, B. A., Schneider, P., Smith, D. J., Stiekema, H., Krone, N., et al. (2011) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2011-1565 · Cited by 458 · see citing works

Gas chromatography/mass spectrometry (GC/MS) remains a pre-eminent discovery tool in clinical steroid investigations even in the era of fast liquid chromatography tandem mass spectrometry (LC/MS/MS)

Krone, N., Hughes, B. A., Lavery, G. G., Stewart, P. M., Arlt, W., & Shackleton, C. H. L. (2010) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2010.04.010 · Cited by 434 · see citing works

Challenges to the measurement of estradiol: an Endocrine Society Position Statement

Rosner, W., Hankinson, S. E., Sluss, P. M., Vesper, H. W., & Wierman, M. E. (2013) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2012-3780 · Cited by 408 · see citing works

Requirement for mass spectrometry sex steroid assays in the journal of clinical endocrinology and metabolism

Handelsman, D. J., & Wartofsky, L. (2013) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2013-3375 · Cited by 368 · see citing works

Liquid Chromatography–Tandem Mass Spectrometry Assay for Simultaneous Measurement of Estradiol and Estrone in Human Plasma

Nelson, R. E., Grebe, S. K. G., O’Kane, D. J., & Singh, R. J. (2004) · Clinical Chemistry · doi:10.1373/clinchem.2003.025478 · Cited by 359 · see citing works

LC-MS/MS in the Clinical Laboratory - Where to From Here?

Grebe, S. K. G., & Singh, R. J. (2011) · Europe PMC (PubMed Central) · Cited by 356 · see citing works

Reference Ranges for Testosterone in Men Generated Using Liquid Chromatography Tandem Mass Spectrometry in a Community-Based Sample of Healthy Nonobese Young Men in the Framingham Heart Study and Applied to Three Geographically Distinct Cohorts

Bhasin, S., Pencina, M., Jasuja, G. K., Travison, T. G., Coviello, A., Orwoll, E., Wang, P. Y., Nielson, C., Wu, F., Tajar, A., et al. (2011) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2010-3012 · Cited by 355 · see citing works

Mass spectrometry and immunoassay: how to measure steroid hormones today and tomorrow

Taylor, A. E., Keevil, B., & Huhtaniemi, I. T. (2015) · European Journal of Endocrinology · doi:10.1530/eje-15-0338 · Cited by 320 · see citing works

Pitfalls Associated with the Use of Liquid Chromatography–Tandem Mass Spectrometry in the Clinical Laboratory

Vogeser, M., & Seger, C. (2010) · Clinical Chemistry · doi:10.1373/clinchem.2009.138602 · Cited by 289 · see citing works

Liquid Chromatography–Tandem Mass Spectrometry Assay for Androstenedione, Dehydroepiandrosterone, and Testosterone with Pediatric and Adult Reference Intervals

Kushnir, M. M., Blamires, T. L., Rockwood, A. L., Roberts, W. L., Yue, B., Erdogan, E., Bunker, A. M., & Meikle, A. W. (2010) · Clinical Chemistry · doi:10.1373/clinchem.2010.143222 · Cited by 282 · see citing works

Hyperandrogenemia Predicts Metabolic Phenotype in Polycystic Ovary Syndrome: The Utility of Serum Androstenedione

O’Reilly, M., TAYLOR, A. E. R., Crabtree, N. J., Hughes, B. A., Capper, F., Crowley, R. K., Stewart, P. M., Tomlinson, J., & Arlt, W. (2014) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2013-3399 · Cited by 279 · see citing works

Steroid Hormone Analysis by Tandem Mass Spectrometry

Soldin, S. J., & Soldin, O. P. (2009) · Clinical Chemistry · doi:10.1373/clinchem.2007.100008 · Cited by 264 · see citing works

Reexamination of testosterone, dihydrotestosterone, estradiol and estrone levels across the menstrual cycle and in postmenopausal women measured by liquid chromatography-tandem mass spectrometry

Rothman, M. S., Carlson, N. E., Xu, M., Wang, C., Swerdloff, R., Lee, P., Goh, V. H. H., Ridgway, E. C., & Wierman, M. E. (2011) · Steroids · doi:10.1016/j.steroids.2010.10.010 · Cited by 248 · see citing works

Clinical steroid mass spectrometry: A 45-year history culminating in HPLC–MS/MS becoming an essential tool for patient diagnosis

Shackleton, C. H. L. (2010) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2010.02.017 · Cited by 227 · see citing works

Liquid chromatography-tandem mass spectrometry analysis of human adrenal vein 19-carbon steroids before and after ACTH stimulation

Rege, J., Nakamura, Y., Satoh, F., Morimoto, R., Kennedy, M. R., Layman, L. C., Honma, S., Sasano, H., & Rainey, W. E. (2013) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2012-2912 · Cited by 227 · see citing works

Liquid chromatography tandem mass spectrometry for analysis of steroids in clinical laboratories

Kushnir, M. M., Rockwood, A. L., Roberts, W. L., Yue, B., Bergquist, J., & Meikle, A. W. (2010) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2010.07.008 · Cited by 226 · see citing works

Salivary cortisone is a potential biomarker for serum free cortisol

Perogamvros, I., Keevil, B. G., Ray, D. W., & Trainer, P. J. (2010) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2010-1215 · Cited by 222 · see citing works

High-Sensitivity Tandem Mass Spectrometry Assay for Serum Estrone and Estradiol

Kushnir, M. M., Rockwood, A. L., Bergquist, J., Varshavsky, M., Roberts, W. L., Yue, B., Bunker, A. M., & Meikle, A. W. (2008) · American Journal of Clinical Pathology · doi:10.1309/lc03bhq5xjpjyekg · Cited by 209 · see citing works

Comparison of Methods to Measure Low Serum Estradiol Levels in Postmenopausal Women

Lee, J. S., Ettinger, B. B., Stanczyk, F. Z., Vittinghoff, E., Hanes, V., Cauley, J. A., Chandler, W., Settlage, J., Beattie, M. S., Folkerd, E. J., et al. (2006) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2005-2378 · Cited by 203 · see citing works

Standardized LC–MS/MS based steroid hormone profile-analysis

Koal, T., Schmiederer, D., Pham‐Tuan, H., Röhring, C., & Rauh, M. (2011) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2011.12.001 · Cited by 198 · see citing works

The Detection of Cortisol in Human Sweat

Russell, E., Koren, G., Rieder, M. J., & Uum, S. H. M. V. (2013) · Therapeutic Drug Monitoring · doi:10.1097/ftd.0b013e31829daa0a · Cited by 195 · see citing works

Adrenal Corticosteroid Biosynthesis, Metabolism, and Action

Arlt, W., & Stewart, P. M. (2005) · Endocrinology and Metabolism Clinics of North America · doi:10.1016/j.ecl.2005.01.002 · Cited by 194 · see citing works

Serum steroid profiling by isotopic dilution-liquid chromatography–mass spectrometry: Comparison with current immunoassays and reference intervals in healthy adults

Fanelli, F., Belluomo, I., Lallo, V. D. D., Cuomo, G., IASIO, R. D., Baccini, M., Casadio, E., Casetta, B., Vicennati, V., Gambineri, A., et al. (2010) · Steroids · doi:10.1016/j.steroids.2010.11.005 · Cited by 190 · see citing works

Utility of salivary cortisol measurements in Cushing's syndrome and adrenal insufficiency

Raff, H. (2009) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2009-1166 · Cited by 186 · see citing works

Age-Specific Reference Ranges for Serum Testosterone and Androstenedione Concentrations in Women Measured by Liquid Chromatography-Tandem Mass Spectrometry

Haring, R., Hannemann, A., John, U., Radke, D., Nauck, M., Wallaschofski, H., Owen, L., Adaway, J., Keevil, B. G., & Brabant, G. (2012) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2011-2134 · Cited by 185 · see citing works

Comparison of salivary cortisol as measured by different immunoassays and tandem mass spectrometry

Miller, R., Plessow, F., Rauh, M., Gröschl, M., & Kirschbaum, C. (2013) · Psychoneuroendocrinology · doi:10.1016/j.psyneuen.2012.04.019 · Cited by 181 · see citing works

An LC-MS/MS method for steroid profiling during adrenal venous sampling for investigation of primary aldosteronism

Peitzsch, M., Dekkers, T., Haase, M., Sweep, F. C. G. J., Quack, I., Antoch, G., Siegert, G., Lenders, J. W. M., Deinum, J., Willenberg, H. S., et al. (2015) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2014.10.006 · Cited by 169 · see citing works

The role of liquid chromatography–tandem mass spectrometry in the clinical laboratory

van den Ouweland, J. M. W., & Kema, I. P. (2011) · Journal of Chromatography B · doi:10.1016/j.jchromb.2011.11.044 · Cited by 160 · see citing works

Mass Spectrometry–Based Adrenal and Peripheral Venous Steroid Profiling for Subtyping Primary Aldosteronism

Eisenhofer, G., Dekkers, T., Peitzsch, M., Dietz, A., Bidlingmaier, M., Treitl, M., Williams, T. A., Bornstein, S. R., Haase, M., Rump, L. C., et al. (2016) · Clinical Chemistry · doi:10.1373/clinchem.2015.251199 · Cited by 159 · see citing works

Superiority of gas chromatography/tandem mass spectrometry assay (GC/MS/MS) for estradiol for monitoring of aromatase inhibitor therapy

Santen, R. J., Demers, L., Ohorodnik, S. K., Settlage, J. A., Langecker, P. J., Blanchett, D., Goss, P. E., & Wang, S. (2007) · Steroids · doi:10.1016/j.steroids.2007.05.003 · Cited by 147 · see citing works

Simultaneous measurement of cortisol and cortisone in human saliva using liquid chromatography-tandem mass spectrometry: application in basal and stimulated conditions

Perogamvros, I., Owen, L. J., Newell-Price, J., Ray, D. W., Trainer, P. J., & Keevil, B. G. (2009) · Journal of Chromatography B · doi:10.1016/j.jchromb.2009.09.014 · Cited by 140 · see citing works

Novel liquid chromatography tandem mass spectrometry (LC-MS/MS) methods for measuring steroids

Keevil, B. G. (2013) · Best Practice & Research Clinical Endocrinology & Metabolism · doi:10.1016/j.beem.2013.05.015 · Cited by 134 · see citing works

18-Hydroxycorticosterone, 18-Hydroxycortisol, and 18-Oxocortisol in the Diagnosis of Primary Aldosteronism and Its Subtypes

Mulatero, P., di Cella, S. M., Monticone, S., Schiavone, D., Manzo, M. L., Mengozzi, G., Rabbia, F., Terzolo, M., Gómez-Sánchez, E. P., Gómez-Sánchez, C. E., et al. (2012) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2011-2384 · Cited by 133 · see citing works

A sensitive, simple and robust LC–MS/MS method for the simultaneous quantification of seven androgen- and estrogen-related steroids in postmenopausal serum

Ke, Y., Bertin, J. O., Gonthier, R., Simard, J.-N., & Labrie, F. (2014) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2014.08.015 · Cited by 130 · see citing works

Measurement of Peripheral Plasma 18-Oxocortisol Can Discriminate Unilateral Adenoma From Bilateral Diseases in Patients With Primary Aldosteronism

Satoh, F., Morimoto, R., Ono, Y., Iwakura, Y., Omata, K., Kudo, M., Takase, K., Seiji, K., Sasamoto, H., Honma, S., et al. (2015) · Hypertension · doi:10.1161/hypertensionaha.114.04453 · Cited by 128 · see citing works

Salivary Cortisone Reflects Cortisol Exposure Under Physiological Conditions and After Hydrocortisone

Debono, M., Harrison, R. F., Whitaker, M. J., Eckland, D., Arlt, W., Keevil, B. G., & Ross, R. J. (2016) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2015-3694 · Cited by 128 · see citing works

Quantitative analysis of estradiol and six other steroid hormones in human saliva using a high throughput liquid chromatography–tandem mass spectrometry assay

Gao, W., Tobias Stalder, A., & Kirschbaum, C. (2015) · Talanta · doi:10.1016/j.talanta.2015.05.004 · Cited by 127 · see citing works

Development of a highly sensitive method for the quantification of estrone and estradiol in serum by liquid chromatography tandem mass spectrometry without derivatization

Fiers, T., Casetta, B., Bernaert, B., Vandersypt, E., Debock, M., & Kaufman, J. (2012) · Journal of Chromatography B · doi:10.1016/j.jchromb.2012.02.034 · Cited by 127 · see citing works

Development and validation of a quantitative assay based on tandem mass spectrometry

Honour, J. W. (2011) · Annals of Clinical Biochemistry International Journal of Laboratory Medicine · doi:10.1258/acb.2010.010176 · Cited by 126 · see citing works

Fast and sensitive liquid chromatography–mass spectrometry assay for seven androgenic and progestagenic steroids in human serum

Keski‐Rahkonen, P., Huhtinen, K., POUTANEN, M. H., & Auriola, S. O. K. (2011) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2011.06.006 · Cited by 117 · see citing works

A critical evaluation of salivary testosterone as a method for the assessment of serum testosterone

Fiers, T., Delanghe, J., T'Sjoen, G. G., Caenegem, E. V., Wierckx, K., & Kaufman, J. (2014) · Steroids · doi:10.1016/j.steroids.2014.04.013 · Cited by 111 · see citing works

Comparison of serum cortisol measurement by immunoassay and liquid chromatography-tandem mass spectrometry in patients receiving the 11 <i>β</i> -hydroxylase inhibitor metyrapone

Monaghan, P. J., Owen, L. J., Trainer, P. J., Brabant, G., Keevil, B. G., & Darby, D. (2011) · Annals of Clinical Biochemistry International Journal of Laboratory Medicine · doi:10.1258/acb.2011.011014 · Cited by 111 · see citing works

Use of LC-MS/MS for the Open Detection of Steroid Metabolites Conjugated with Glucuronic Acid

Fabregat, A., Pozo, Ó. J., Marcos, J., Segura, J., & Ventura, R. (2013) · Analytical Chemistry · doi:10.1021/ac4001749 · Cited by 106 · see citing works

Simultaneous Measurement of Thirteen Steroid Hormones in Women with Polycystic Ovary Syndrome and Control Women Using Liquid Chromatography-Tandem Mass Spectrometry

Keefe, C. C., Goldman, M. M., Zhang, K., Clarke, N. J., Reitz, R. E., & Welt, C. K. (2014) · PLoS ONE · doi:10.1371/journal.pone.0093805 · Cited by 105 · see citing works

Testosterone to Dihydrotestosterone Ratio as a New Biomarker for an Adverse Metabolic Phenotype in the Polycystic Ovary Syndrome

Münzker, J., Hofer, D., Trummer, C., Ulbing, M., Harger, A., Pieber, T. R., Owen, L. J., Keevil, B. G., Brabant, G. E., Lerchbaum, E., et al. (2014) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2014-2523 · Cited by 104 · see citing works

Evaluation of steroidomics by liquid chromatography hyphenated to mass spectrometry as a powerful analytical strategy for measuring human steroid perturbations

Jeanneret, F., Tonoli, D., Rossier, M. F., Saugy, M., Boccard, J., & Rudaz, S. L. (2015) · Journal of Chromatography A · doi:10.1016/j.chroma.2015.07.008 · Cited by 100 · see citing works

Salivary testosterone measurement by liquid chromatography tandem mass spectrometry in adult males and females

Keevil, B. G., Macdonald, P. R., Macdowall, W. G., Lee, D. M., Wu, F. C. W., & the NATSAL Team (2013) · Annals of Clinical Biochemistry International Journal of Laboratory Medicine · doi:10.1177/0004563213506412 · Cited by 99 · see citing works

Isotope-Dilution Liquid Chromatography–Tandem Mass Spectrometry Candidate Reference Method for Total Testosterone in Human Serum

Botelho, J. C., Shacklady, C., Cooper, H. C., Tai, S. S.-C., Uytfanghe, K. V., Thienpont, L. M., & Vesper, H. W. (2012) · Clinical Chemistry · doi:10.1373/clinchem.2012.190934 · Cited by 98 · see citing works

Liquid chromatography tandem mass spectrometry in the clinical laboratory

Adaway, J., Keevil, B. G., & Owen, L. J. (2014) · Annals of Clinical Biochemistry International Journal of Laboratory Medicine · doi:10.1177/0004563214557678 · Cited by 95 · see citing works

Multisteroid LC–MS/MS assay for glucocorticoids and androgens and its application in Addison's disease

Methlie, P., Hustad, S., Kellman, R., Almås, B., Erichsen, M. M., Husebye, E. S., & Løvås, K. (2013) · Endocrine Connections · doi:10.1530/ec-13-0023 · Cited by 92 · see citing works

The coming of age of liquid chromatography coupled to tandem mass spectrometry in the endocrinology laboratory

Carvalho, V. M. (2012) · Journal of Chromatography B · doi:10.1016/j.jchromb.2011.08.027 · Cited by 90 · see citing works

Performance enhancement in the measurement of 5 endogenous steroids by LC-MS/MS combined with differential ion mobility spectrometry

Ray, J. A., Kushnir, M. M., Yost, R. A., Rockwood, A. L., & Wayne Meikle, A. (2015) · Clinica Chimica Acta · doi:10.1016/j.cca.2014.07.036 · Cited by 88 · see citing works

Determination of aldosterone in serum by liquid chromatography–tandem mass spectrometry

Turpeinen, U., Hämäläinen, E. K., & Stenman, U. (2007) · Journal of Chromatography B · doi:10.1016/j.jchromb.2007.11.005 · Cited by 87 · see citing works

Steroid profiling by LC-MS/MS in nonsecreting and subclinical cortisol-secreting adrenocortical adenomas

Di Dalmazi, G., Fanelli, F., Mezzullo, M., Casadio, E., Rinaldi, E., Garelli, S., Giampalma, E., Mosconi, C., Golfieri, R., Vicennati, V., et al. (2015) · The Journal of Clinical Endocrinology &amp; Metabolism · doi:10.1210/jc.2015-1992 · Cited by 82 · see citing works

Sex, age, pubertal development and use of oral contraceptives in relation to serum concentrations of DHEA, DHEAS, 17α-hydroxyprogesterone, δ4-androstenedione, testosterone and their ratios in children, adolescents and young adults

Søeborg, T., Frederiksen, H., Mouritsen, A., Johannsen, T. H., Main, K. M., Jørgensen, N., Petersen, J. H., Andersson, A.-M., & Juul, A. (2014) · Clinica Chimica Acta · doi:10.1016/j.cca.2014.06.018 · Cited by 81 · see citing works

Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) Assay for Simultaneous Measurement of Salivary Testosterone and Cortisol in Healthy Men for Utilization in the Diagnosis of Late-onset Hypogonadism in Males

Matsui, F., Koh, E., Yamamoto, K., SUGIMOTO, K., Sin, H., Maeda, Y., Honma, S., & Namiki, M. (2009) · Endocrine Journal · doi:10.1507/endocrj.k09e-186 · Cited by 80 · see citing works

Investigation of endogenous corticosteroids profiles in human urine based on liquid chromatography tandem mass spectrometry

Marcos, J., Renau, N., Casals, G., Segura, J., Ventura, R., & Pozo, O. J. (2014) · Analytica Chimica Acta · doi:10.1016/j.aca.2013.12.030 · Cited by 76 · see citing works

Comparison of 7 Published LC-MS/MS Methods for the Simultaneous Measurement of Testosterone, Androstenedione, and Dehydroepiandrosterone in Serum

Büttler, R. M., Martens, F., Fanelli, F., Pham, H. T., Kushnir, M. M., Janssen, M. J. W., Owen, L., Taylor, A. E., Soeborg, T., Blankenstein, M. A., et al. (2015) · Clinical Chemistry · doi:10.1373/clinchem.2015.242859 · Cited by 76 · see citing works

Quantitation of aldosterone in human plasma by ultra high performance liquid chromatography tandem mass spectrometry

Hinchliffe, E., Carter, S., Owen, L. J., & Keevil, B. G. (2012) · Journal of Chromatography B · doi:10.1016/j.jchromb.2012.11.013 · Cited by 72 · see citing works

Simultaneous measurement of cyclosporin A and tacrolimus from dried blood spots by ultra high performance liquid chromatography tandem mass spectrometry

Hinchliffe, E., Adaway, J., & Keevil, B. G. (2011) · Journal of Chromatography B · doi:10.1016/j.jchromb.2011.05.016 · Cited by 71 · see citing works

Enhancement of specificity of aldosterone measurement in human serum and plasma using 2D-LC–MS/MS and comparison with commercial immunoassays

Ray, J. A., Kushnir, M. M., Palmer, J., Sadjadi, S., Rockwood, A. L., & Meikle, A. W. (2014) · Journal of Chromatography B · doi:10.1016/j.jchromb.2014.08.042 · Cited by 71 · see citing works

Simultaneous analysis of cortisol and cortisone in saliva using XLC–MS/MS for fully automated online solid phase extraction

Jones, R. L., Owen, L. J., Adaway, J. E., & Keevil, B. G. (2012) · Journal of Chromatography B · doi:10.1016/j.jchromb.2011.11.036 · Cited by 69 · see citing works

A sensitive and rapid mass spectrometric method for the simultaneous measurement of eight steroid hormones and CALIPER pediatric reference intervals

Kyriakopoulou, L., Yazdanpanah, M., Colantonio, D. A., Chan, M. K., Daly, C. H., & Adeli, K. (2013) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2013.01.002 · Cited by 68 · see citing works

Urine Free cortisol in the diagnosis of cushing’s syndrome: Is It worth doing and, If so, how?

Raff, H., Auchus, R. J., Findling, J. W., & Nieman, L. K. (2015) · The Journal of Clinical Endocrinology &amp; Metabolism · doi:10.1210/jc.2014-3766 · Cited by 68 · see citing works

Mass spectrometry techniques in the survey of steroid metabolites as potential disease biomarkers: A review

Gouveia, M. J., Brindley, P. J., Santos, L. L., da Costa, J. M. C., Gomes, P. A. C., & Vale, N. (2013) · Metabolism · doi:10.1016/j.metabol.2013.04.003 · Cited by 68 · see citing works

Specificity and predictive value of circulating testosterone assessed by tandem mass spectrometry for the diagnosis of polycystic ovary syndrome by the National Institutes of Health 1990 criteria

Salameh, W. A., Goldman, M. M., Clarke, N. J., Mathur, R., Azziz, R., & Reitz, R. E. (2014) · Fertility and Sterility · doi:10.1016/j.fertnstert.2013.12.056 · Cited by 67 · see citing works

Improved profiling of estrogen metabolites by orbitrap LC/MS

Li, X., & Franke, A. A. (2014) · Steroids · doi:10.1016/j.steroids.2014.12.005 · Cited by 66 · see citing works

Simultaneous determination of hair cortisol, cortisone and DHEAS with liquid chromatography–electrospray ionization-tandem mass spectrometry in negative mode

Chen, Z., Li, J., Zhang, J., Xing, X., Gao, W., Lu, Z., & Deng, H. (2013) · Journal of Chromatography B · doi:10.1016/j.jchromb.2013.04.026 · Cited by 64 · see citing works

Profiling intact steroid sulfates and unconjugated steroids in biological fluids by liquid chromatography-tandem mass spectrometry (LC-MS-MS)

Galuska, C. E., Hartmann, M. F., Sánchez-Guijo, A., Bakhaus, K., Geyer, J., Schuler, G., Zimmer, K.-P., & Wudy, S. A. (2013) · The Analyst · doi:10.1039/c3an36817c · Cited by 64 · see citing works

Measurement of salivary cortisol with liquid chromatography-tandem mass spectrometry in patients undergoing dynamic endocrine testing

Perogamvros, I., Owen, L. J., Keevil, B. G., Brabant, G., & Trainer, P. J. (2010) · Clinical Endocrinology · doi:10.1111/j.1365-2265.2009.03582.x · Cited by 61 · see citing works

Serum concentrations of DHEA, DHEAS, 17α-hydroxyprogesterone, Δ4-androstenedione and testosterone in children determined by TurboFlow-LC–MS/MS

Søeborg, T., Frederiksen, H. W., Fruekilde, P. B. N., Johannsen, T. H., Juul, A., & Andersson, A. (2013) · Clinica Chimica Acta · doi:10.1016/j.cca.2013.01.019 · Cited by 61 · see citing works

Ultrasensitive quantification of serum estrogens in postmenopausal women and older men by liquid chromatography–tandem mass spectrometry

Wang, Q., Rangiah, K., Mesaros, C., Snyder, N. W., Vachani, A., Song, H., & Blair, I. A. (2015) · Steroids · doi:10.1016/j.steroids.2015.01.014 · Cited by 58 · see citing works

Current LC–MS methods and procedures applied to the identification of new steroid metabolites

Marcos, J., & Pozo, Ó. J. (2015) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2015.12.012 · Cited by 58 · see citing works

Measurement of late-night salivary cortisol and cortisone by LC-MS/MS to assess preanalytical sample contamination with topical hydrocortisone

Raff, H., & Singh, R. J. (2012) · Clinical Chemistry · doi:10.1373/clinchem.2012.182717 · Cited by 57 · see citing works

A purification step prior to commercial sensitive immunoassay is necessary to achieve clinical usefulness when quantifying serum 17β-estradiol in prepubertal children

Ankarberg‐Lindgren, C., & Norjavaara, E. (2008) · European Journal of Endocrinology · doi:10.1530/eje-07-0403 · Cited by 57 · see citing works

The role of salivary cortisol measured by liquid chromatography-tandem mass spectrometry in the diagnosis of subclinical hypercortisolism

Palmieri, S., Morelli, V, Polledri, E., Fustinoni, S., Mercadante, R., Olgiati, L., Eller Vainicher, C., Cairoli, E., Zhukouskaya, V. V., Beck-Peccoz, P., et al. (2013) · European Journal of Endocrinology · doi:10.1530/eje-12-0803 · Cited by 57 · see citing works

Steroid profiling for congenital adrenal hyperplasia by tandem mass spectrometry as a second-tier test reduces follow-up burdens in a tertiary care hospital: a retrospective and prospective evaluation

Seo, J. Y., Park, H.-D., Kim, J. W., Oh, H. J., Yang, J. S., Chang, Y. S., Park, W. S., & Lee, S.-Y. (2014) · jpme · doi:10.1515/jpm-2013-0154 · Cited by 55 · see citing works

Determination of serum aldosterone by liquid chromatography and tandem mass spectrometry: a liquid–liquid extraction method for the ABSCIEX API-5000 mass spectrometry system

van der Gugten, J. G., Dubland, J. A., Liu, H., Wang, A. Y., Joseph, C., & Holmes, D. T. (2012) · Journal of Clinical Pathology · doi:10.1136/jclinpath-2011-200564 · Cited by 55 · see citing works

Interlaboratory Variation in 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 Is Significantly Improved If Common Calibration Material Is Used

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Liquid chromatography tandem mass spectrometry determination of free and conjugated estrogens in breast cancer patients before and after exemestane treatment

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Late‐night salivary cortisol for diagnosis of Cushing’s syndrome by liquid chromatography/tandem mass spectrometry assay

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Estradiol assays – The path ahead

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Development, validation, and certification by isotope dilution gas chromatography-mass spectrometry of lyophilized human serum reference materials for cortisol (CRM 192 and 193) and progesterone (CRM 347 and 348)

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Development of a rapid assay for the analysis of serum cortisol and its implementation into a routine service laboratory

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Editorial:The New Instructions to Authors for the Reporting of Steroid Hormone Measurements

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A rapid direct assay for the routine measurement of oestradiol and oestrone by liquid chromatography tandem mass spectrometry

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Validated LC–MS/MS simultaneous assay of five sex steroid/neurosteroid-related sulfates in human serum

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Testosterone measurement by liquid chromatography tandem mass spectrometry: the importance of internal standard choice

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Novel Fragmentation Pathways of Anionic Adducts of Steroids Formed by Electrospray Anion Attachment Involving Regioselective Attachment, Regiospecific Decompositions, Charge-Induced Pathways, and Ion–Dipole Complex Intermediates

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Case for the wider adoption of mass spectrometry-based adrenal steroid testing, and beyond

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Determination of salivary testosterone and androstendione by liquid chromatography–tandem mass spectrometry

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Simultaneous measurement of testosterone, androstenedione and dehydroepiandrosterone (DHEA) in serum and plasma using Isotope-Dilution 2-Dimension Ultra High Performance Liquid-Chromatography Tandem Mass Spectrometry (ID-LC–MS/MS)

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Direct measurement of free estradiol in human serum by equilibrium dialysis–liquid chromatography–tandem mass spectrometry and reference intervals of free estradiol in women

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Editorial: The New Instructions to Authors for the Reporting of Steroid Hormone Measurements

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Supported liquid extraction as an alternative to solid phase extraction for LC-MS/MS aldosterone analysis?

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Separation of dansylated 17β-estradiol, 17α-estradiol, and estrone on a single HPLC column for simultaneous quantitation by LC–MS/MS

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LC–MS/MS Method for the Simultaneous Determination of Free Urinary Steroids

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Candidate reference measurement procedure for the quantification of total serum cortisol with LC-MS/MS

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Precursor-to-product ratios reflect biochemical phenotype in congenital adrenal hyperplasia

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A rapid and sensitive UPLC–MS/MS method for the simultaneous quantification of serum androsterone glucuronide, etiocholanolone glucuronide, and androstan-3α, 17β diol 17-glucuronide in postmenopausal women

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Steroids excreted in urine by neonates with 21-hydroxylase deficiency: Characterization, using GC–MS and GC–MS/MS, of the D-ring and side chain structure of pregnanes and pregnenes

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Measurement of dehydroepiandrosterone sulphate (DHEAS): A comparison of Isotope-Dilution Liquid Chromatography Tandem Mass Spectrometry (ID-LC-MS/MS) and seven currently available immunoassays

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The use of mass spectrometry to improve the diagnosis and the management of the HPA axis

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A simple LC–MS/MS method for the determination of cortisol, cortisone and tetrahydro-metabolites in human urine: Assay development, validation and application in depression patients

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Androgen glucuronides analysis by liquid chromatography tandem-mass spectrometry: could it raise new perspectives in the diagnostic field of hormone-dependent malignancies?

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Revisiting hyper- and hypo-androgenism by tandem mass spectrometry

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A comprehensive study for the validation of a LC–MS/MS method for the determination of free and total forms of urinary cortisol and its metabolites

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Salivary testosterone in female-to-male transgender adolescents during treatment with intra-muscular injectable testosterone esters

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Salivary cortisol and cortisone responses to tetracosactrin (synacthen)

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Mass Spectrometry in the Clinical Laboratory: How Have We Done, and Where Do We Need to Be?

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Discrepancy between radioimmunoassay and high performance liquid chromatography tandem-mass spectrometry for the analysis of androstenedione

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Stable Composition of the Nano- and Picoplankton Community during the Ocean Iron Fertilization Experiment LOHAFEX

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Quantification of unbound prednisolone, prednisone, cortisol and cortisone in human plasma by ultrafiltration and direct injection into liquid chromatrography tandem mass spectrometry

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Use of Micro-HPLC-MS/MS Method to Assess Diurnal Effects on Steroid Hormones

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Assessment of salivary free cortisol levels by liquid chromatography with tandem mass spectrometry (LC-MS/MS) in patients treated with mitotane

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Development and application of a high-throughput sample cleanup process based on 96-well plate for simultaneous determination of 16 steroids in biological matrices using liquid chromatography–triple quadrupole mass spectrometry

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Adrenal hormonal imbalance in acute intermittent porphyria patients: results of a case control study

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Methodological approach to the intracrine study and estimation of DHEA and DHEA-S using liquid chromatography–tandem mass spectrometry (LC–MS/MS)

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New Strategies for Detecting Steroid Metabolic Disorders—Paneling vs Profiling

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After another decade: LC–MS/MS became routine in clinical diagnostics

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11-Oxygenated C19 steroids are the predominant androgens in polycystic ovary syndrome

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Liquid chromatography–tandem mass spectrometry for clinical diagnostics

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Intracrine androgen biosynthesis, metabolism and action revisited

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Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LC-MS/MS

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New Cutoffs for the Biochemical Diagnosis of Adrenal Insufficiency after ACTH Stimulation using Specific Cortisol Assays

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Cortisol as a Stress Indicator in Fish: Sampling Methods, Analytical Techniques, and Organic Pollutant Exposure Assessments

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Pubertal induction and transition to adult sex hormone replacement in patients with congenital pituitary or gonadal reproductive hormone deficiency: an Endo-ERN clinical practice guideline

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Perfluoroalkyl substances and sex hormones in postmenopausal women: NHANES 2013–2016

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Analysis by LC–MS/MS of endogenous steroids from human serum, plasma, endometrium and endometriotic tissue

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Simultaneous analysis by LC–MS/MS of 22 ketosteroids with hydroxylamine derivatization and underivatized estradiol from human plasma, serum and prostate tissue

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GC/MS in recent years has defined the Normal and clinically disordered steroidome: will It Soon Be surpassed by LC/Tandem MS in this role?

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From a single steroid to the steroidome: Trends and analytical challenges

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Review of the Use of Liquid Chromatography-Tandem Mass Spectrometry in Clinical Laboratories: Part II–Operations

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New 3D-printed sorbent for extraction of steroids from human plasma preceding LC–MS analysis

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Plasma and serum oxylipin, endocannabinoid, bile acid, steroid, fatty acid and nonsteroidal anti-inflammatory drug quantification in a 96-well plate format

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The utility of ultra-high performance supercritical fluid chromatography–tandem mass spectrometry (UHPSFC-MS/MS) for clinically relevant steroid analysis

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An LC-MS/MS analysis for seven sex hormones in serum

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Interpretation of common endocrine laboratory tests: technical pitfalls, their mechanisms and practical considerations

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Sex- and age-specific reference intervals of 16 steroid metabolites quantified simultaneously by LC-MS/MS in sera from 2458 healthy subjects aged 0 to 77 years

Frederiksen, H., Johannsen, T. H., Andersen, S. E., Petersen, J. H., Busch, A. S., Ljubicic, M. L., Fischer, M. B., Upners, E. N., Hagen, C. P., Main, K. M., et al. (2024) · Clinica Chimica Acta · doi:10.1016/j.cca.2024.119852 · Cited by 46 · see citing works

Analysis types and quantification methods applied in UHPLC-MS metabolomics research: a tutorial

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Measurement of Salivary Adrenal-Specific Androgens as Biomarkers of Therapy Control in 21-Hydroxylase Deficiency

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Breast Cancer: Targeting of Steroid Hormones in Cancerogenesis and Diagnostics

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Parallel targeted and non-targeted quantitative analysis of steroids in human serum and peritoneal fluid by liquid chromatography high-resolution mass spectrometry

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Female and male serum reference intervals for challenging sex and precursor steroids by liquid chromatography - tandem mass spectrometry

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Parallel diurnal fluctuation of testosterone, androstenedione, dehydroepiandrosterone and 17OHprogesterone as assessed in serum and saliva: validation of a novel liquid chromatography-tandem mass spectrometry method for salivary steroid profiling

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Steroid metabolism in breast cancer: Where are we and what are we missing?

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Development and validation of a LC-MS/MS method for the establishment of reference intervals and biological variation for five plasma steroid hormones

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Total saponins extracted from Abrus cantoniensis Hance suppress hepatitis B virus replication in vitro and in rAAV8-1.3HBV transfected mice

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A multiplex protein panel assay for severity prediction and outcome prognosis in patients with COVID-19: An observational multi-cohort study

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Doping control analysis of 13 steroids and structural-like analytes in human urine using Quadrupole-Orbitrap LC–MS/MS with parallel reaction monitoring (PRM) mode

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Simultaneous measurement of 18 steroids in human and mouse serum by liquid chromatography–mass spectrometry without derivatization to profile the classical and alternate pathways of androgen synthesis and metabolism

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Steroid metabolome profiling of follicular fluid in normo- and hyperandrogenic women with polycystic ovary syndrome

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Insight on the Intracrinology of Menopause: Androgen Production within the Human Vagina

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Lipidomics analysis in drug discovery and development.

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Steroid Mass Spectrometry for the Diagnosis of PCOS

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Mass spectrometry or immunoassay: est modus in rebus

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Multiplexed analysis of steroid hormones in saliva by LC-MS/MS with 2-hydrazinopyridine derivatization

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The Bisphenol A Induced Oxidative Stress in Non-Alcoholic Fatty Liver Disease Male Patients: A Clinical Strategy to Antagonize the Progression of the Disease

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Selective LC-MRM/SIM-MS based profiling of adrenal steroids reveals metabolic signatures of 17α-hydroxylase deficiency

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Fully Automated Quantitative Measurement of Serum Organic Acids via LC-MS/MS for the Diagnosis of Organic Acidemias: Establishment of an Automation System and a Proof-of-Concept Validation

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Insights into the response mechanism of Litopenaeus vannamei exposed to cold stress during live transport combining untargeted metabolomics and biochemical assays

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A sensitive and robust UPLC–MS/MS method for quantitation of estrogens and progestogens in human serum

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Glycolithocholic acid increases the frequency of circulating Tregs through constitutive androstane receptor to alleviate postmenopausal osteoporosis

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Measurement of serum steroid profiles by HPLC-tandem mass spectrometry

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Quantitative MALDI-MS assay of steroid hormones in plasma based on hydroxylamine derivatization

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A high resolution LC–MS targeted method for the concomitant analysis of 11 contraceptive progestins and 4 steroids

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The comprehensive characterization of adrenocortical steroidogenesis using two-dimensional ultra-performance liquid chromatography – electrospray ionization tandem mass spectrometry

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Analysis of non-conjugated steroids in water using paper spray mass spectrometry

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History of androgens and androgen action

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The evolution of methods for urinary steroid metabolomics in clinical investigations particularly in childhood

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Metabolomic analysis of untargeted bovine uterine secretions in dairy cows with endometritis using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry

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Towards Improved Steroid Hormone Analysis: The Shift from Immunoassays to Mass Spectrometry

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The Effect of Soy Isoflavones on Steroid Metabolism

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LC-MS/MS based quantification of steroidal biomarkers in polycystic ovary syndrome induced rats

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Mass Spectrometry Analysis for Clinical Applications: A Review

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The hypothalamic-pituitary-adrenal axis in the perinatal period: Its relationship with major depressive disorder and early life adversity

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Metabolic Alteration Analysis of Steroid Hormones in Niemann–Pick Disease Type C Model Cell Using Liquid Chromatography/Tandem Mass Spectrometry

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Steroidogenesis in patients with adrenal incidentalomas: Extended steroid profile measured by liquid chromatography‐mass spectrometry after ACTH stimulation and dexamethasone suppression

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Evaluation of various approaches to the isolation of steroid hormones from urine samples prior to FASS‐MEKC analysis

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Maternal postpartum depressive symptoms: The predictive role of objective and subjective birth experience and hair glucocorticoids

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Development and validation of a deep eutectic solvent-assisted liquid-liquid extraction method for simultaneous quantification of six steroid hormones in serum by liquid chromatography-tandem mass spectrometry

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The dynamics of a serum steroid profile after stimulation with intravenous ACTH

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Mass Spectrometry-Based Steroid Profiling: Meeting Unmet Clinical Needs

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Development and validation of a liquid chromatography–tandem mass spectrometry method for the simultaneous analysis of androgens, estrogens, glucocorticoids and progestagens in human serum

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Simultaneous quantification of progestogens in plasma and serum by UHPLC-HRMS employing multiplexed targeted single ion monitoring

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Monitoring Farmed Fish Welfare by Measurement of Cortisol as a Stress Marker in Fish Feces by Liquid Chromatography Coupled with Tandem Mass Spectrometry

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Targeted metabolic profiling of urinary steroids with a focus on analytical accuracy and sample stability

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Semi-automated serum steroid profiling with tandem mass spectrometry

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A sensitive, precise and rapid LC–MS/MS method for determination of ergosterol peroxide in Paecilomyces cicadae mycelium

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Common Pitfalls in the Interpretation of Endocrine Tests

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Cortisol and Dexamethasone Mediate Glucocorticoid Actions in the Lesser Spotted Catshark (Scyliorhinus canicula)

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A tandem liquid chromatography and tandem mass spectrometry (LC/LC–MS/MS) technique to separate and quantify steroid isomers 11β-methyl-19-nortestosterone and testosterone

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Waterless live transport degrades the flesh quality of Litopenaeus vannamei by disturbing neuroendocrine response: based on physiology and metabolomics

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Operation Moonshot: rapid translation of a SARS-CoV-2 targeted peptide immunoaffinity liquid chromatography-tandem mass spectrometry test from research into routine clinical use

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Optimizing charge state distribution is a prerequisite for accurate protein biomarker quantification with LC-MS/MS, as illustrated by hepcidin measurement

Schmitz, E. M. H., Leijten, N. M., van Dongen, J. L. J., Broeren, M. A. C., Milroy, L. G., Brunsveld, L., Scharnhorst, V., & van de Kerkhof, D. (2018) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm-2018-0013 · Cited by 9 · see citing works

Changes in Adrenal Androgens and Steroidogenic Enzyme Activities From Ages 2, 4, to 6 Years: A Prospective Cohort Study

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The contribution of serum cortisone and glucocorticoid metabolites to detrimental bone health in patients receiving hydrocortisone therapy

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Metabolic Variation Dictates Cardiac Pathogenesis in Patients With Tetralogy of Fallot

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Phosphate Flame Retardants and Plasticizers and Their Association with Reproductive and Steroid Hormone Levels among Peripubertal-Aged Children: The Hokkaido Birth Cohort Study

Ikeda, A., Yamaguchi, T., Bamai, Y. A., Roggeman, M., Ikenaka, Y., Zeng, Y., Yasuda, A., Ketema, R. M., Marsela, M., Gys, C., et al. (2025) · Environmental Science & Technology · doi:10.1021/acs.est.4c11436 · Cited by 8 · see citing works

Extended Steroid Profiling in Human Serum and Plasma With Simultaneous Quantitative Determination Using One‐Point Internal Calibration

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Development and functionalization of electrospun fiber coated thin film microextraction devices for rapid mass spectrometric determination of biologically important polar molecules

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RNA-seq and LC-MS/MS analysis of antiviral effects mediated by cold stress and stress hormone corticosterone in chicken DF-1 cells

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Development of Noninvasive Method for the Automated Analysis of Nine Steroid Hormones in Human Saliva by Online Coupling of In-Tube Solid-Phase Microextraction with Liquid Chromatography–Tandem Mass Spectrometry

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Measuring steroids from dried blood spots using tandem mass spectrometry to diagnose congenital adrenal hyperplasia

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Patient centric blood sampling and analysis for diagnostics and laboratory medicine

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False elevation of serum cortisol in chemiluminescence immunoassay by Siemens Advia Centaur XP system in 21-hydroxylase deficiency: an ‘endocrine laboma’

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Evaluation of five multisteroid LC‒MS/MS methods used for routine clinical analysis: comparable performance was obtained for nine analytes

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A novel defensin‐like peptide C‐13326 possesses protective effect against multidrug‐resistant Aeromonas schubertii in hybrid snakehead (Channa maculate ♀ × Channa argus ♂)

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Evaluation of a commercial liquid-chromatography high-resolution mass-spectrometry method for the determination of hepcidin-25

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A decade in female reproduction: an endocrine view of the past and into the future

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Steroid analysis in clinical routine diagnostics – discussing crucial questions

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Challenges and Benefits of Endogenous Steroid Analysis by LC–MS/MS

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