Cited by 219Hydrogels: synthesis, properties, applications3D Printing in Biomedical ResearchCellular Mechanics and Interactions

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Gelatine methacrylamide-based hydrogels: An alternative three-dimensional cancer cell culture system

Kaemmerer, Elke, Melchels, Ferry P.W., Holzapfel, Boris M., Meckel, Tobias, Hutmacher, Dietmar W., Loessner, Daniela·2014·Acta Biomaterialia

DOI 10.1016/j.actbio.2014.02.035

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Abstract

Modern cancer research requires physiological, three-dimensional (3-D) cell culture platforms, wherein the physical and chemical characteristics of the extracellular matrix (ECM) can be modified. In this study, gelatine methacrylamide (GelMA)-based hydrogels were characterized and established as in vitro and in vivo spheroid-based models for ovarian cancer, reflecting the advanced disease stage of patients, with accumulation of multicellular spheroids in the tumour fluid (ascites). Polymer concentration (2.5-7% w/v) strongly influenced hydrogel stiffness (0.5±0.2kPa to 9.0±1.8kPa) but had little effect on solute diffusion. The diffusion coefficient of 70kDa fluorescein isothiocyanate (FITC)-labelled dextran in 7% GelMA-based hydrogels was only 2.3 times slower compared to water. Hydrogels of medium concentration (5% w/v GelMA) and stiffness (3.4kPa) allowed spheroid formation and high proliferation and metabolic rates. The inhibition of matrix metalloproteinases and consequently ECM degradability reduced spheroid formation and proliferation rates. The incorporation of the ECM components laminin-411 and hyaluronic acid further stimulated spheroid growth within GelMA-based hydrogels. The feasibility of pre-cultured GelMA-based hydrogels as spheroid carriers within an ovarian cancer animal model was proven and led to tumour development and metastasis. These tumours were sensitive to treatment with the anti-cancer drug paclitaxel, but not the integrin antagonist ATN-161. While paclitaxel and its combination with ATN-161 resulted in a treatment response of 33-37.8%, ATN-161 alone had no effect on tumour growth and peritoneal spread. The semi-synthetic biomaterial GelMA combines relevant natural cues with tunable properties, providing an alternative, bioengineered 3-D cancer cell culture in in vitro and in vivo model systems.

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Fiji: an open-source platform for biological-image analysis
Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., et al. (2012) · Nature Methods · doi:10.1038/nmeth.2019 · Cited by 66,890
Fabrication of novel biomaterials through molecular self-assembly
Zhang, S. (2003) · Nature Biotechnology · doi:10.1038/nbt874 · Cited by 3,375
The third dimension bridges the gap between cell culture and live tissue
Pampaloni, F., Reynaud, E. G., & Stelzer, E. H. K. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2236 · Cited by 2,817
Cell-laden microengineered gelatin methacrylate hydrogels
Nichol, J. W., Koshy, S. T., Bae, H., Hwang, C. M., Yamanlar, S., & Khademhosseini, A. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.03.064 · Cited by 2,377
Capturing complex 3D tissue physiology in vitro
Griffith, L. G., & Swartz, M. A. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1858 · Cited by 2,347
Matrix Metalloproteinases in Vascular Remodeling and Atherogenesis
Galis, Z. S., & Khatri, J. J. (2002) · Circulation Research · doi:10.1161/res.90.3.251 · Cited by 1,965
Determination of subcutaneous tumor size in athymic (nude) mice
Tomayko, M. M., & Reynolds, C. P. (1989) · Cancer Chemotherapy and Pharmacology · doi:10.1007/bf00300234 · Cited by 1,830
Matrix metalloproteinases in vascular remodeling and atherogenesis: the good, the bad, and the ugly.
Galis, Z. S., & Khatri, J. J. (2002) · PubMed · Cited by 1,787
Modeling Tissue Morphogenesis and Cancer in 3D
Yamada, K. M., & Cukierman, E. (2007) · Cell · doi:10.1016/j.cell.2007.08.006 · Cited by 1,751
Multicellular tumor spheroids: An underestimated tool is catching up again
Hirschhaeuser, F., Menne, H., Dittfeld, C., West, J., Mueller-Klieser, W., & Kunz-Schughart, L. A. (2010) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2010.01.012 · Cited by 1,674

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Hydrogels for tissue engineering and regenerative medicine
Hunt, J. A., Chen, R., van Veen, T., & Bryan, N. (2014) · Journal of Materials Chemistry B · doi:10.1039/c4tb00775a · Cited by 378
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Authors: Kaemmerer, E., Melchels, F. P. W., Holzapfel, B. M., Meckel, T., Hutmacher, D. W., & Loessner, D.
Affiliations: Queensland University of Technology (AU)
Technische Universität Darmstadt (DE)
Corresponding author: Loessner, Daniela
ORCID: Melchels, Ferry P.W.
Holzapfel, Boris M.
Meckel, Tobias
Hutmacher, Dietmar W.
Loessner, Daniela
Venue: Acta Biomaterialia
Publisher: Elsevier BV
Published: 2014-06
Volume: 10
Issue: 6
Pages: 2551-2562
ISSN: 1742-7061 (ISSN-L)
DOI: 10.1016/j.actbio.2014.02.035
OpenAlex: W2091262660
Cited by: 219
References: 75
Citations by year: 2026: 8 · 2025: 16 · 2024: 17 · 2023: 24 · 2022: 26 · 2021: 18 · 2020: 15 · 2019: 24 · 2018: 15 · 2017: 18 · 2016: 19 · 2015: 13 · 2014: 5
FWCI: 8.39
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Acta Biomaterialia (publishedVersion)
PubMed (publishedVersion)
Griffith Research Online (submittedVersion)
Funders: Australian Research Council
the Cancer Council Queensland (PIOF-GA-2010-272286, HO 5068/1-1)
Cells and Tissue Domain Collaborative Research Development
Institute of Health and Biomedical Innovation
Queensland University of Technology, Australia
Marie Curie Fellowship
German Research Foundation
Topics: Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords: hydrogels, ovarian cancer, tumor spheroids, ascites, hydrogel stiffness, solute diffusion, extracellular matrix, matrix metalloproteinases, laminin-411, hyaluronic acid, paclitaxel, integrin antagonists, peritoneal carcinomatosis, biomaterials
MeSH terms: Acrylamides, Cell Division, Cells, Cultured, Extracellular Matrix, Gelatin, Humans, Hydrogels, Matrix Metalloproteinases, Cell Line, Tumor

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Gelatine methacrylamide-based hydrogels: An alternative three-dimensional cancer cell culture system

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