The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
In vitro fermentation and prebiotic potential of novel low molecular weight polysaccharides derived from agar and alginate seaweeds
Abstract
Fermentation properties and prebiotic potential of novel low molecular weight polysaccharides (LMWPs) derived from agar and alginate bearing seaweeds was investigated. Ten LMWPs were supplemented to pH, temperature controlled anaerobic batch cultures inoculated with human feces from three donors, in triplicate. Microbiota changes were monitored using Fluorescent in-situ hybridization and short chain fatty acids, the fermentation end products were analysed using gas chromatography. Of the ten LMWPs tested, Gelidium seaweed CC2253 of molecular weight 64.64 KDa showed a significant increase in bifidobacterial populations from log(10) 8.06 at 0 h to log(10) 8.55 at 24 h (p = 0.018). For total bacterial populations, alginate powder CC2238 produced a significant increase from log(10) 9.01 at 0 h to log(10) 9.58 at 24 h (p = 0.032). No changes were observed in the other bacterial groups tested viz. Bacteroides, Lactobacilli/Enterococci, Eubacterium rectale/Clostridium coccoides and Clostridium histolyticum. The polysaccharides also showed significant increases in total SCFA production, particularly acetic and propionic acids, indicating that they were readily fermented. In conclusion, some LMWPs derived from agar and alginate bearing seaweeds were fermented by gut bacteria and exhibited potential to be used a novel source of prebiotics.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“One of the most important nutritionally relevant components of seaweeds are polysaccharides, most of them cannot be digested by humans, and thus, they can be considered as dietary fiber (33%–75% of the total composition) [96,97]. In turn, soluble fractions account between 50% and 85% of total dietary fiber content [96].”
From The Potential of Seaweeds as a Source of Functional Ingredients of Prebiotic and Antioxidant Value (2019) · cites this paper as [97]
Citations
Cites
Dietary Modulation of the Human Colonic Microbiota: Introducing the Concept of Prebiotics
Gibson, G. R., & Roberfroid, M. B. (1995) · Journal of Nutrition · doi:10.1093/jn/125.6.1401 · Cited by 7,659
Daims, H., Brühl, A., Amann, R., SCHLEIFER, K. H., & Wagner, M. (1999) · Systematic and Applied Microbiology · doi:10.1016/s0723-2020(99)80053-8 · Cited by 2,273
Manz, W., Amann, R., Ludwig, W., Vancanneyt, M., & SCHLEIFER, K. H. (1996) · Microbiology · doi:10.1099/13500872-142-5-1097 · Cited by 1,176
Transfer of carbohydrate-active enzymes from marine bacteria to Japanese gut microbiota
Hehemann, J.-H., Correc, G., Barbeyron, T., Helbert, W., Czjzek, M., & Michel, G. (2010) · Nature · doi:10.1038/nature08937 · Cited by 1,117
Franks, A. H., Harmsen, H. J. M., Raangs, G. C., Jansen, G. J., Schut, F., & Welling, G. W. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.9.3336-3345.1998 · Cited by 1,056
Nutritional Value of Edible Seaweeds
MacArtain, P., Gill, C. I. R., Brooks, M., Campbell, R., & Rowland, I. R. (2008) · Nutrition Reviews · doi:10.1111/j.1753-4887.2007.tb00278.x · Cited by 976
Self-citationLangendijk-Genevaux, P. S., Schut, F., Jansen, G. J., Raangs, G. C., Kamphuis, G. R., Wilkinson, M. H. F., & Welling, G. W. (1995) · Applied and Environmental Microbiology · doi:10.1128/aem.61.8.3069-3075.1995 · Cited by 974
Walker, A. W., Duncan, S. H., McWilliam Leitch, E. C., Child, M. W., & Flint, H. J. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.7.3692-3700.2005 · Cited by 796
Wang, X., & Gibson, G. R. (1993) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1993.tb02790.x · Cited by 659
Jiménez‐Escrig, A., & Sánchez-Muniz, F. J. (2000) · Nutrition Research · doi:10.1016/s0271-5317(00)00149-4 · Cited by 476
Fermentation of mucins and plant polysaccharides by anaerobic bacteria from the human colon
Salyers, A. A., West, S. E. H., Vercellotti, J. R., & Wilkins, T. D. (1977) · Applied and Environmental Microbiology · doi:10.1128/aem.34.5.529-533.1977 · Cited by 471
Harmsen, P. E. H. J. M., Elfferich, P., Schut, F., & Welling, G. W. (1999) · Microbial Ecology in Health and Disease · doi:10.1080/089106099435862 · Cited by 376
Laere, K. M. J. V., Hartemink, R., Bosveld, M., Schols, H. A., & Voragen, A. G. J. (2000) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf990519i · Cited by 345
Richardson, A. J., Calder, A. G., Stewart, C. S., & Smith, A. C. (1989) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.1989.tb00278.x · Cited by 326
Hughes, S. A., Shewry, P. R., Gibson, G. R., McCleary, B. V., & Rastall, R. A. (2008) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.2008.00478.x · Cited by 276
In Vitro Fermentation by Human Fecal Microflora of Wheat Arabinoxylans
Hughes, S. A., Shewry, P. R., Li, L., Gibson, G. R., Sanz, M. L., & Rastall, R. A. (2007) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf070293g · Cited by 274
In vitro fermentability of dextran, oligodextran and maltodextrin by human gut bacteria
Olano-Martin, E., Mountzouris, K. C., Gibson, G. R., & Rastall, R. A. (2000) · British Journal Of Nutrition · doi:10.1017/s0007114500000325 · Cited by 236
Study on the effects of laminarin, a polysaccharide from seaweed, on gut characteristics
Devillé, C., Gharbi, M., Dandrifosse, G., & Peulen, O. (2007) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.2901 · Cited by 184
Carbohydrate Fermentation, Energy Transduction and Gas Metabolism in the Human Large Intestine
Macfarlane, G. T., & Gibson, G. R. (1997) · Springer eBooks · doi:10.1007/978-1-4615-4111-0_9 · Cited by 174
Salazar, N., Ruas-Madiedo, P., Kolida, S., Collins, M., Rastall, R., Gibson, G., & de los Reyes-Gavilán, C. G. (2009) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2009.08.017 · Cited by 168
Functional intestinal microbiome, new frontiers in prebiotic design
Candela, M., Maccaferri, S., Turroni, S., Carnevali, P., & Brigidi, P. (2010) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2010.04.017 · Cited by 153
Laminarin in the dietary fibre concept
Devillé, C., Damas, J., Forget, P., Dandrifosse, G., & Peulen, O. (2004) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.1754 · Cited by 146
In vitro comparison of the prebiotic effects of two inulin-type fructans
Pompei, A., Cordisco, L., Raimondi, S., Amaretti, A., PAGNONI, U. M., Matteuzzi, D., & Rossi, M. (2008) · Anaerobe · doi:10.1016/j.anaerobe.2008.07.002 · Cited by 142
Altamimi, M., Palframan, R. J., Cooper, J. M., Gibson, G. R., & Rastall, R. A. (2005) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2005.02780.x · Cited by 131
Ramnani, P., Gaudier, E. A., Bingham, M. O., van Bruggen, P., Tuohy, K. M., & Gibson, G. R. (2010) · British Journal Of Nutrition · doi:10.1017/s000711451000036x · Cited by 121
Self-citationMichel, C., Lahaye, M., Bonnet, C., Mabeau, S., & Barry, J. L. (1996) · British Journal Of Nutrition · doi:10.1079/bjn19960129 · Cited by 118
Dierick, N. A., Ovyn, A., & SMET, S. D. (2009) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.3480 · Cited by 111
Microbial Digestion of Complex Carbohydrates in Man
Cummings, J. H. (1984) · Proceedings of The Nutrition Society · doi:10.1079/pns19840025 · Cited by 106
In Vitro Fermentation of Oat Flours from Typical and High β-Glucan Oat Lines
Kim, H. J., & White, P. J. (2009) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf900788c · Cited by 74
In vitro fermentation and hydration properties of commercial dietary fiber-rich supplements
Goñi, I., & Martín‐Carrón, N. (1998) · Nutrition Research · doi:10.1016/s0271-5317(98)00090-6 · Cited by 60
Forest, V. F., Clément, M., Pierre, F. H. F., Méflah, K., & Ménanteau, J. (2003) · Nutrition and Cancer · doi:10.1207/s15327914nc4501_10 · Cited by 38
Wu, S.-C., Wang, F.-J., & Pan, C. (2007) · Journal of marine science and technology · doi:10.51400/2709-6998.2038 · Cited by 24
Research works printed but not linked: 1
- Warrand, 2006, Healthy polysaccharides, Food Technol Biotechnol, 44, 355
Cited by
Sorted by citations
Role of the normal gut microbiota
Jandhyala, S. M. (2015) · World Journal of Gastroenterology · doi:10.3748/wjg.v21.i29.8787 · Cited by 3,001
Human gut microbiota/microbiome in health and diseases: a review
Gomaa, E. Z. (2020) · Antonie van Leeuwenhoek · doi:10.1007/s10482-020-01474-7 · Cited by 1,294
Factors affecting the composition of the gut microbiota, and its modulation
Hasan, N., & Yang, H. (2019) · PeerJ · doi:10.7717/peerj.7502 · Cited by 807
Nondigestible carbohydrates, butyrate, and butyrate-producing bacteria
Fu, X., Liu, Z., Zhu, C., Mou, H., & Kong, Q. (2019) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2018.1542587 · Cited by 497
Risks and benefits of consuming edible seaweeds
Cherry, P., O’Hara, C., Magee, P. J., McSorley, E. M., & Allsopp, P. J. (2019) · Nutrition Reviews · doi:10.1093/nutrit/nuy066 · Cited by 440
Metadata
- Authors
- Ramnani, P., Chitarrari, R., Tuohy, K., Grant, J., Hotchkiss, S., Philp, K., Campbell, R., Gill, C., & Rowland, I.
- Affiliations
- University of Reading (GB)
- Fondazione Edmund Mach (IT)
- CyberColloids (IE)
- University of Ulster (GB)
- Corresponding author
- Ramnani, Priya
- Venue
- Anaerobe
- Publisher
- Elsevier BV
- Published
- 2012-02
- Volume
- 18
- Issue
- 1
- Pages
- 1-6
- ISSN
- 1075-9964 (ISSN-L)
- OpenAlex
- W2145123908
- Cited by
- 252
- References
- 32
- Citations by year
- 2026: 10 · 2025: 19 · 2024: 19 · 2023: 17 · 2022: 23 · 2021: 21 · 2020: 26 · 2019: 20 · 2018: 16 · 2017: 21 · 2016: 19 · 2015: 13 · 2014: 13 · 2013: 6 · 2012: 9
- FWCI
- 13.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Anaerobe (publishedVersion)
- PubMed (publishedVersion)
- CINECA IRIS Institutional Research Information System (Fondazione Edmund Mach) (submittedVersion)
- Funders
- European Commission
- Research Executive Agency
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- low molecular weight polysaccharides, agar, alginate, seaweed, prebiotics, human fecal microbiota, short-chain fatty acids, Bifidobacterium, anaerobic batch culture, Gelidium, Bacteroides, Clostridium histolyticum
- MeSH terms
- Adult, Agar, Fatty Acids, Volatile, Feces, Female, Fermentation, Humans, Male, Molecular Weight, Polysaccharides, Seaweed, Time Factors, Gastrointestinal Tract, Metagenome, Prebiotics, Batch Cell Culture Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
