Effect of the ionophore monensin and tannin extracts supplemented to grass silage on populations of ruminal cellulolytics and methanogens in vitro

Witzig, M.; Zeder, M.; Rodehutscord, M.

doi:10.1016/j.anaerobe.2018.01.012

Cited by 33Ruminant Nutrition and Digestive PhysiologyAnaerobic Digestion and Biogas ProductionAnimal Nutrition and Physiology

Effect of the ionophore monensin and tannin extracts supplemented to grass silage on populations of ruminal cellulolytics and methanogens in vitro

Witzig, M., Zeder, M., Rodehutscord, M.·2018·Anaerobe

DOI 10.1016/j.anaerobe.2018.01.012

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Abstract

This study examined whether the methane-decreasing effect of monensin (∼21%) and different hydrolysable tannins (24%-65%) during in vitro fermentation of grass silage was accompanied by changes in abundances of cellulolytics and methanogens. Samples of liquid (LAM) and solid (SAM) associated microbes were obtained from two rumen simulation technique experiments in which grass silage was either tested in combination with monensin (0, 2 or 4 mg d-1) or with different tannin extracts from chestnut, valonea, sumac and grape seed (0 or 1.5 g d-1). Total prokaryotes were quantified by 4',6-diamidino-2-phenylindol (DAPI) staining of paraformaldehyde-ethanol-fixed cells and relative abundances of ruminal cellulolytic and methanogenic species were assessed by real time quantitative PCR. Results revealed no change in absolute numbers of prokaryotic cells with monensin treatment, neither in LAM nor in SAM. By contrast, supplementation of chestnut and grape seed tannins decreased total prokaryotic counts compared to control. However, relative abundances of total methanogens did not differ between tannin treatments. Thus, the decreased methane production by 65% and 24% observed for chestnut and grape seed tannins, respectively, may have been caused by a lower total number of methanogens, but methane production seemed to be also dependent on changes in the microbial community composition. While the relative abundance of F. succinogenes decreased with monensin addition, chestnut and valonea tannins inhibited R. albus. Moreover, a decline in relative abundances of Methanobrevibacter sp., especially M. ruminantium, and Methanosphaera stadtmanae was shown with supplementation of monensin or chestnut tannins. Proportions of Methanomicrobium mobile were decreased by monensin in LAM while chestnut and valonea had an increasing effect on this methanogenic species. Our results demonstrate a different impact of monensin and tannins on ruminal cellulolytics and gave indication that methane decrease by monensin and chestnut tannins was associated with decreased abundances of M. ruminantium and M. stadtmanae.

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CH4 emission is reduced in response to monensin and chestnut tannin supplementation, owing to lower abundances of M. ruminantium and M. stadtmanae.
“According to Witzig et al. (184), CH4 emission was reduced in response to monensin and chestnut tannin supplementation, owing to the lower abundances of M. ruminantium and M. stadtmanae.”
From Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction (2020) · cites this paper as [184]

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Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction
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Authors: Witzig, M., Zeder, M., & Rodehutscord, M.
Affiliations: University of Hohenheim (DE)
Laboratoire de physique des systèmes complexes (FR)
Corresponding author: Witzig, M.
ORCID: Rodehutscord, M.
Venue: Anaerobe
Publisher: Elsevier BV
Published: 2018-04
Volume: 50
Pages: 44-54
ISSN: 1075-9964 (ISSN-L)
DOI: 10.1016/j.anaerobe.2018.01.012
OpenAlex: W2792341262
Cited by: 33
References: 93
Citations by year: 2026: 2 · 2025: 2 · 2024: 6 · 2023: 4 · 2022: 3 · 2021: 6 · 2020: 5 · 2019: 4 · 2018: 1
FWCI: 6.76
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Anaerobe (publishedVersion)
PubMed (publishedVersion)
Funders: H. Wilhelm Schaumann Stiftung
Topics: Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
Keywords: monensin, tannins, grass silage, rumen fermentation, methane mitigation, Methanobrevibacter ruminantium, Methanosphaera stadtmanae, Fibrobacter succinogenes, Ruminococcus albus, chestnut tannin, grape seed tannins, RUSITEC, microbial community composition
MeSH terms: Animals, Animal Feed, Bacteria, Cellulose, Digestion, Fermentation, Poaceae, Ionophores, Methane, Monensin, Rumen, Silage, Tannins, Dietary Supplements, Biodiversity, Microbiota

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Effect of the ionophore monensin and tannin extracts supplemented to grass silage on populations of ruminal cellulolytics and methanogens in vitro

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