Cited by 15Lipid Membrane Structure and BehaviorFatty Acid Research and HealthLipid metabolism and biosynthesis

Fatty acid-based lipidomics and membrane remodeling induced by apoE3 and apoE4 in human neuroblastoma cells

Open access
Prasinou, Paraskevi, Dafnis, Ioannis, Giacometti, Giorgia, Ferreri, Carla, Chroni, Angeliki, Chatgilialoglu, Chryssostomos·2017·Biochimica et Biophysica Acta (BBA) - Biomembranes

Abstract

Apolipoprotein E (apoE) is a major lipid carrier of the lipoprotein transport system that plays critical roles in various pathologies. Human apoE has three common isoforms, the apoE4 being associated with Alzheimer's disease. This is the first study in the literature investigating the effects of apoE (apoE3 and apoE4 isoforms) on membrane fatty acid profile in neuroblastoma SK-N-SH cells. Fatty acid analyses were carried out by gas chromatography of the corresponding methyl esters (FAME). We observed the occurrence of membrane fatty acid remodeling in the presence of each of the two apoE isoforms. ApoE3 increased the membrane level of stearic acid and dihomo-gamma-linolenic acid (DGLA), whereas apoE4 had opposite effects. Both apoE3 and apoE4 increased saturated and monounsaturated fatty acids (SFA and MUFA), omega-6/omega-3 ratio and decreased total polyunsaturated fatty acid (PUFA) amount, but with various intensities. Moreover, both apoE isoforms decreased membrane homeostasis indexes such as PUFA balance, unsaturation index and peroxidation index. Our results highlight membrane property changes connected to the apoE isoforms suggesting membrane lipidomics to be inserted in further model studies of apolipoproteins in health and disease.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.bbamem.2017.07.001

Cites

60 of 60 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The ApoE-lipoprotein complex has been shown to alter the lipid profile of cancer and noncancerous cells.

    “The ApoE-lipoprotein complex has been shown to alter the lipid profile of cancer and noncancerous cells (47–49).”

    From Secreted Apoe rewires melanoma cell state vulnerability to ferroptosis (2024) · cites this paper as [49]

    The Results and discussion report that apoE3 and apoE4 altered membrane fatty-acid composition in SK-N-SH neuroblastoma cells. The experiment used lipid-free apoE, not an ApoE–lipoprotein complex, and did not test noncancerous cells, so those parts of the claim are not supported by this paper. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

1 retracted

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Prasinou, P., Dafnis, I., Giacometti, G., Ferreri, C., Chroni, A., & Chatgilialoglu, C.
Affiliations
  • National Centre of Scientific Research "Demokritos" (GR)
  • Institute of Organic Synthesis and Photoreactivity (IT)
Corresponding author
Chatgilialoglu, Chryssostomos
Venue
Biochimica et Biophysica Acta (BBA) - Biomembranes
Publisher
Elsevier BV
Published
2017-10
Volume
1859
Issue
10
Pages
1967-1973
ISSN
0005-2736 (ISSN-L)
OpenAlex
W2732473219
Cited by
15
References
60
Citations by year
2024: 3 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 1 · 2019: 4 · 2018: 1 · 2017: 1
FWCI
0.78
Type
journal article
Language
en
Open access
Locations
  • Biochimica et Biophysica Acta (BBA) - Biomembranes (publishedVersion)
  • PubMed (publishedVersion)
  • Archivio istituzionale della ricerca (Alma Mater Studiorum Università di Bologna) (submittedVersion)
Funders
  • EU COST Action CM1201
  • Marie Skłodowska-Curie European Training Network (H2020-MSCA-ETN-2014-642023)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
apolipoprotein E, APOE3, APOE ε4, neuroblastoma cells, SK-N-SH cells, stearic acid, dihomo-gamma-linolenic acid, n-6/n-3 ratio, polyunsaturated fatty acids, lipid peroxidation, Alzheimer's disease, apolipoproteins
MeSH terms
Alzheimer Disease, Fatty Acids, Fatty Acids, Monounsaturated, Homeostasis, Humans, Membranes, Neuroblastoma, Stearic Acids, 8,11,14-Eicosatrienoic Acid, Protein Isoforms, Cell Line, Tumor, Apolipoprotein E3, Apolipoprotein E4

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review