Fatty acid-based lipidomics and membrane remodeling induced by apoE3 and apoE4 in human neuroblastoma cells
Open accessAbstract
Apolipoprotein E (apoE) is a major lipid carrier of the lipoprotein transport system that plays critical roles in various pathologies. Human apoE has three common isoforms, the apoE4 being associated with Alzheimer's disease. This is the first study in the literature investigating the effects of apoE (apoE3 and apoE4 isoforms) on membrane fatty acid profile in neuroblastoma SK-N-SH cells. Fatty acid analyses were carried out by gas chromatography of the corresponding methyl esters (FAME). We observed the occurrence of membrane fatty acid remodeling in the presence of each of the two apoE isoforms. ApoE3 increased the membrane level of stearic acid and dihomo-gamma-linolenic acid (DGLA), whereas apoE4 had opposite effects. Both apoE3 and apoE4 increased saturated and monounsaturated fatty acids (SFA and MUFA), omega-6/omega-3 ratio and decreased total polyunsaturated fatty acid (PUFA) amount, but with various intensities. Moreover, both apoE isoforms decreased membrane homeostasis indexes such as PUFA balance, unsaturation index and peroxidation index. Our results highlight membrane property changes connected to the apoE isoforms suggesting membrane lipidomics to be inserted in further model studies of apolipoproteins in health and disease.
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The ApoE-lipoprotein complex has been shown to alter the lipid profile of cancer and noncancerous cells.
“The ApoE-lipoprotein complex has been shown to alter the lipid profile of cancer and noncancerous cells (47–49).”
From Secreted Apoe rewires melanoma cell state vulnerability to ferroptosis (2024) · cites this paper as [49]
The Results and discussion report that apoE3 and apoE4 altered membrane fatty-acid composition in SK-N-SH neuroblastoma cells. The experiment used lipid-free apoE, not an ApoE–lipoprotein complex, and did not test noncancerous cells, so those parts of the claim are not supported by this paper. (LLM, conf=0.88)
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- Authors
- Prasinou, P., Dafnis, I., Giacometti, G., Ferreri, C., Chroni, A., & Chatgilialoglu, C.
- Affiliations
- National Centre of Scientific Research "Demokritos" (GR)
- Institute of Organic Synthesis and Photoreactivity (IT)
- Corresponding author
- Chatgilialoglu, Chryssostomos
- Venue
- Biochimica et Biophysica Acta (BBA) - Biomembranes
- Publisher
- Elsevier BV
- Published
- 2017-10
- Volume
- 1859
- Issue
- 10
- Pages
- 1967-1973
- ISSN
- 0005-2736 (ISSN-L)
- OpenAlex
- W2732473219
- Cited by
- 15
- References
- 60
- Citations by year
- 2024: 3 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 1 · 2019: 4 · 2018: 1 · 2017: 1
- FWCI
- 0.78
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biochimica et Biophysica Acta (BBA) - Biomembranes (publishedVersion)
- PubMed (publishedVersion)
- Archivio istituzionale della ricerca (Alma Mater Studiorum Università di Bologna) (submittedVersion)
- Funders
- EU COST Action CM1201
- Marie Skłodowska-Curie European Training Network (H2020-MSCA-ETN-2014-642023)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- apolipoprotein E, APOE3, APOE ε4, neuroblastoma cells, SK-N-SH cells, stearic acid, dihomo-gamma-linolenic acid, n-6/n-3 ratio, polyunsaturated fatty acids, lipid peroxidation, Alzheimer's disease, apolipoproteins
- MeSH terms
- Alzheimer Disease, Fatty Acids, Fatty Acids, Monounsaturated, Homeostasis, Humans, Membranes, Neuroblastoma, Stearic Acids, 8,11,14-Eicosatrienoic Acid, Protein Isoforms, Cell Line, Tumor, Apolipoprotein E3, Apolipoprotein E4
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