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Cited by 125Exercise and Physiological ResponsesImmune Response and InflammationCardiovascular and exercise physiology

Toll-like receptor expression on classic and pro-inflammatory blood monocytes after acute exercise in humans

Simpson, Richard J., McFarlin, Brian K., McSporran, Carrie, Spielmann, Guillaume, Hartaigh, Bríain ó, Guy, Keith·2009·Brain Behavior and Immunity
DOI 10.1016/j.bbi.2008.09.013
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Abstract

Monocytes are a heterogeneous group of cells, the relative distribution of which change in peripheral blood following a strenuous bout of aerobic exercise. Monocyte subtypes can be identified in blood based on the cell surface expression of CD14 and CD16: classic (CD14(++bright)/CD16(-negative)) and the CD16(+dim) (CD14(++bright)/CD16(+dim)) and CD16(++bright) (CD14(+dim)/CD16(++bright)) pro-inflammatory subtypes. Whole monocyte population changes in TLR2, TLR4 and HLA.DR expression have previously been documented after acute exercise without accounting for relative changes in monocyte subpopulations, therefore, this study examined their expression on classic and pro-inflammatory monocyte subsets following 45min of treadmill running at 75% VO(2max). Mononuclear cells isolated from the peripheral blood of moderately trained male subjects (n=15) before (PRE), immediately after (POST) and 1h after (1H) exercise were assessed for TLR2, TLR4 and HLA.DR expression on blood monocytes and their subpopulations using three-colour flow cytometry. Compared to PRE, the proportion of CD14+/CD16+ monocytes was 27% greater POST and 49% less at 1H and was associated with changes in the CD16(++bright) pro-inflammatory subtype (p<0.05). TLR2 expression was 12% lower on CD16(+dim) monocytes POST (p<0.05), whereas TLR4 and HLA.DR expression on total monocytes was 12% and 22% lower at 1H, respectively, and was attributed to changes in the classic (p<0.05) and not the pro-inflammatory subsets (p>0.05). We conclude that acute exercise causes localised changes in TLR2, TLR4 and HLA.DR expression within specific blood monocyte subpopulations, and could therefore be occurring at the cellular level. Such alterations might have significant implications for modulation of post-exercise immune surveillance.

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  • A single bout of treadmill running resulted in an immediate but transient increase in circulating CD8+ T cells and proinflammatory monocytes in moderately trained male subjects in their mid-20s.

    “Moreover, a single bout of treadmill running resulted in an immediate but transient increase in circulating CD8+ T cells (42) and proinflammatory monocytes (43) in moderately trained male subjects in their mid-20s.”

    From Exercise Counters the Age-Related Accumulation of Senescent Cells (2022) · cites this paper as [43]

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Authors
Simpson, R. J., McFarlin, B. K., McSporran, C., Spielmann, G., Hartaigh, B. Ó., & Guy, K.
Affiliations
  • Edinburgh Napier University (GB)
  • University of Houston (US)
Corresponding author
Simpson, Richard J.
ORCID
Venue
Brain Behavior and Immunity
Publisher
Elsevier BV
Published
2009-02
Volume
23
Issue
2
Pages
232-239
ISSN
0889-1591 (ISSN-L)
DOI
10.1016/j.bbi.2008.09.013
OpenAlex
W1974048619
Cited by
125
References
23
Citations by year
2026: 1 · 2025: 4 · 2024: 4 · 2023: 18 · 2022: 7 · 2021: 4 · 2020: 8 · 2019: 4 · 2018: 10 · 2017: 10 · 2016: 7 · 2015: 7 · 2014: 11 · 2013: 8 · 2012: 6
FWCI
5.70
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Brain Behavior and Immunity (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
Keywords
TLR2, TLR4, HLA-DR, monocyte subpopulations, CD14, CD16, acute exercise, treadmill running, monocyte heterogeneity, aerobic exercise
MeSH terms
Adult, Analysis of Variance, Antigens, Surface, Cell Separation, Flow Cytometry, HLA-DR Antigens, Humans, Leukocyte Count, Male, Monocytes, Oxygen Consumption, Time Factors, Biomarkers, Exercise, Receptors, IgG, Lipopolysaccharide Receptors, Toll-Like Receptors, Toll-Like Receptor 2, Toll-Like Receptor 4, Young Adult

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