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Cited by 213Exercise and Physiological ResponsesImmune Response and InflammationCardiovascular and exercise physiology

Influence of exercise training and age on CD14+ cell-surface expression of toll-like receptor 2 and 4

Stewart, Laura K., Flynn, Michael G., Campbell, Wayne W., Craig, Bruce A., Robinson, J. Paul, McFarlin, Brian K., Timmerman, Kyle L., Coen, Paul M. +2 more·2005·Brain Behavior and Immunity
DOI 10.1016/j.bbi.2005.04.003
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Abstract

The influence of an exercise training program and age on inflammatory cytokine production and CD14+cell-surface expression of toll-like receptor 2 (TLR2) and toll-like receptor 4 (TLR4) was examined in 60 younger and older subjects. Subjects were assigned to: young physically active (YPA, n = 15; 25.2 +/- 5.0 years), young physically inactive (YPI, n = 14; 24.9 +/- 4.7 years), older physically active (OPA, n = 14; 71.2 +/- 4.4 years) or older physically inactive (OPI, n = 17; 71.0 +/- 4.3 years) groups. YPI and OPI completed 12 weeks (3 days/week) of endurance (20 min) and resistance exercise (eight exercises, two sets). YPA and OPA groups were instructed to continue their normal activity for 12 weeks. Blood was collected at rest, before and after the 12-week training and control period. A whole blood method was used to determine lipopolysaccharide-(LPS) and peptidoglycan-(PGN) stimulated IL-6, IL-1beta, and TNF-alpha production with supernatants analyzed using ELISA. CD14+ cell-surface expression of TLR2 and TLR4 were measured using flow cytometry. Training increased estimated VO(2max) by 10.4% and increased strength by an average of 38.1%. YPI and OPI had a post-training reduction in LPS-stimulated IL-6 production (P < .01), but LPS-stimulated IL-1beta and TNF-alpha and PGN-stimulated cytokines were not changed. CD14+ cell TLR4 was significantly reduced (P < .05) in YPI and OPI groups after training, but TLR2 was not significantly changed. An exercise training program reduced LPS-stimulated IL-6, concomitant with lower TLR4. These results provide further support for a training- or physical activity-induced lowering of TLR4 and inflammation.

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Authors
Stewart, L. K., Flynn, M. G., Campbell, W. W., Craig, B. A., Robinson, J. P., McFarlin, B. K., Timmerman, K. L., Coen, P. M., Felker, J., & Talbert, E.
Affiliations
  • Purdue University West Lafayette (US)
  • University of Houston (US)
Corresponding author
Flynn, Michael G.
ORCID
Venue
Brain Behavior and Immunity
Publisher
Elsevier BV
Published
2005-09
Volume
19
Issue
5
Pages
389-397
ISSN
0889-1591 (ISSN-L)
DOI
10.1016/j.bbi.2005.04.003
OpenAlex
W2062539824
Cited by
213
References
40
Citations by year
2026: 9 · 2025: 6 · 2024: 12 · 2023: 8 · 2022: 12 · 2021: 8 · 2020: 15 · 2019: 5 · 2018: 13 · 2017: 14 · 2016: 15 · 2015: 7 · 2014: 12 · 2013: 13 · 2012: 7
FWCI
8.08
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Brain Behavior and Immunity (publishedVersion)
  • PubMed (publishedVersion)
Funders
American Heart Association
Topics
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
Keywords
exercise training, TLR2, TLR4, CD14+ cells, age, physical activity, inflammation, inflammatory cytokines, interleukin-6, lipopolysaccharide, peptidoglycan, resistance training, endurance exercise, older adults, VO2max
MeSH terms
Adolescent, Adult, Aged, Aged, 80 and over, Aging, Female, Fluorescent Dyes, Humans, Lipopolysaccharides, Longitudinal Studies, Male, Membrane Glycoproteins, Middle Aged, Peptidoglycan, Physical Endurance, Physical Fitness, Receptors, Cell Surface, Stimulation, Chemical, Weight Lifting, Anaerobic Threshold, Cytokines, Muscle, Skeletal, Lipopolysaccharide Receptors, Toll-Like Receptors, Toll-Like Receptor 2, Toll-Like Receptor 4

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