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Cited by 33Exercise and Physiological ResponsesCardiovascular and exercise physiologyT-cell and B-cell Immunology

Acute aerobic exercise in humans increases cytokine expression in CD27− but not CD27+ CD8+ T-cells

LaVoy, Emily C., Bosch, Jos A., Lowder, Thomas W., Simpson, Richard J.·2013·Brain, Behavior, and Immunity
DOI 10.1016/j.bbi.2012.09.006
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Abstract

Exercise alters the percentage of CD8(+) T-cells in the bloodstream expressing type I and type II cytokines. It is unknown if this reflects a change in cytokine expression within individual cells, or whether these observations result from the exercise-induced shift in the proportions of early/intermediate (CD27(+)) and late (CD27(-)) differentiated cells, which have vastly different cytokine profiles. 16 males cycled for 60 min at 95% maximal steady state. Mononuclear cells isolated from blood collected before, immediately after, and 1 h after exercise were cultured overnight with and without phytohaemagglutinin stimulation. CD8(+) T-cells were assessed for differentiation markers and intracellular cytokine expression by flow cytometry. The numbers and percentage of CD27(-)CD8(+) T-cells increased immediately after exercise and fell below pre-exercise values 1 h later. At 1 h after exercise, an increased number and percentage of CD8(+) T-cells expressing IL-2, IFN-γ, TNF-α, IL-6, IL-4, and IL-10 was observed in both stimulated and unstimulated cells. The cytokine response to exercise was confined to CD27(-)CD8(+) T-cells, although cytokine expression among CD8(+) T-cells was highest when the proportion of CD27(-)CD8(+) T-cells was lowest. Moreover, the cytokine response to exercise could be predicted by the number of late cells in resting blood: cytokine expression was highest among those with low resting proportions of late cells. We conclude that exercise-induced changes in the percentage of CD8(+) T-cells expressing cytokines are not due to proportional shifts in early/intermediate and late differentiated T-cells. Exercise may prime late-differentiated blood CD8(+) T-cells to initiate effector functions in preparation for their extravasation into the tissues.

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  • Measurements of lymphocyte function are strongly influenced by the numbers and proportions of cell types comprising the total lymphocyte pool at the time of sampling, highlighting the need for measurements to be made on a single-cell or single-phenotype basis.

    “Further, measurements of lymphocyte function for example, are strongly influenced by the numbers and proportions of cell types comprising the total lymphocyte pool at the time of sampling, highlighting the need for measurements to be made on a single-cell or single-phenotype basis (Lavoy et al. 2013).”

    From Is immunosenescence influenced by our lifetime “dose” of exercise? (2016) · cites this paper as Lavoy et al. 2013

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Authors
LaVoy, E. C., Bosch, J. A., Lowder, T. W., & Simpson, R. J.
Affiliations
University of Houston (US)
Corresponding author
Simpson, Richard J.
ORCID
Venue
Brain, Behavior, and Immunity
Publisher
Elsevier BV
Published
2013-01
Volume
27
Issue
1
Pages
54-62
ISSN
0889-1591 (ISSN-L)
DOI
10.1016/j.bbi.2012.09.006
OpenAlex
W2016634657
Cited by
33
References
39
Citations by year
2025: 2 · 2024: 1 · 2023: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 4 · 2017: 4 · 2016: 10 · 2015: 1 · 2014: 3 · 2013: 3
FWCI
2.37
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Brain Behavior and Immunity (publishedVersion)
  • PubMed (publishedVersion)
  • UvA-DARE (University of Amsterdam) (publishedVersion)
  • Pure Amsterdam UMC (submittedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
Topics
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Keywords
acute aerobic exercise, CD8+ T cells, CD27, T cell differentiation, cytokine expression, intracellular cytokines, interleukin-2, interferon-gamma, TNF-α, interleukin-6, IL-4, IL-10, exercise immunology, mononuclear cells, phytohemagglutinin stimulation, T cell effector function
MeSH terms
Adult, Cell Differentiation, Flow Cytometry, Humans, Interferon-gamma, Interleukin-2, Male, T-Lymphocytes, Tumor Necrosis Factor-alpha, Exercise, Interleukin-4, Interleukin-6, Cytokines, Interleukin-10, Tumor Necrosis Factor Receptor Superfamily, Member 7, CD8-Positive T-Lymphocytes

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