Hippocampal c-Fos activation in normal and LPA1-null mice after two object recognition tasks with different memory demands
Abstract
Normal and LPA₁-null mice, that have well reported hippocampal deficits, were assessed in an episodic-like what-when-where memory task or in a comparable task designed to test memory for familiar objects and locations by discriminating them from novels. Both genotypes performed the novelty recognition task but failed to learn the what-when-where task. However, normal mice showed what-when memory that was impaired in nulls. Each task elicited a different pattern of c-Fos expression. In normal mice, the what-when-where task induced more hippocampal c-Fos activation in the CA1 area than the novelty-based task, correlating with the what-when memory. LPA₁-null mice displayed a basal c-Fos hyperactivity in the hippocampus and in the medial prefrontal cortex, which was regulated differently by the two behavioural tasks employed. Both tasks were matched in exploratory behaviour and c-Fos activation in stress-related brain areas for both genotypes. This study shows that the what-when-where memory task differs from a comparable novelty-based task in both the learning demands and the neuronal correlates. Moreover, results also stress the role of the LPA₁ receptor in hippocampal functioning.
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The replicability of the ELM2-2 and ELM2-1 tests is verified by independent laboratories.
“The replicability of the ELM2–2 and ELM2–1 tests is verified by independent laboratories (Barbosa et al., 2013; Castilla-Ortega et al., 2012; Castilla-Ortega et al., 2014; Drieskens et al., 2017; Fernandez and Garner, 2008; Inostroza et al., 2013a; Inostroza et al., 2013b; Lanté et al., 2015; Li and Chao, 2008; Lopez-Pigozzi et al., 2016; Wang et al., 2010).”
From The medial prefrontal cortex - hippocampus circuit that integrates information of object, place and time to construct episodic memory in rodents: Behavioral, anatomical and neurochemical properties (2020) · cites this paper as Castilla-Ortega et al., 2012
In the ELM2-1 test, c-fos expression in hippocampal CA1 and dentate gyrus required for episodic-like memory was correlated with the when and where components, respectively.
“the c-fos expression of hippocampal CA1 and dentate gyrus required for episodic-like memory was correlated with the when and where components, respectively”
From The medial prefrontal cortex - hippocampus circuit that integrates information of object, place and time to construct episodic memory in rodents: Behavioral, anatomical and neurochemical properties (2020) · cites this paper as Castilla-Ortega et al., 2012
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“In this respect, Castilla-Ortega et al. (2012) studied c-Fos activation after an ELM task in wild-type mice and LPA1-null mice.”
BackgroundResultInfluentialc-Fos expression correlates with performance on novel object and novel place recognition tests
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- Authors
- Castilla-Ortega, E., Pedraza, C., Chun, J., Fonseca, F. R. D., Estivill-Torrús, G., & Santín, L. J.
- Affiliations
- Universidad de Málaga (ES)
- Scripps Research Institute (US)
- Corresponding author
- Santín, Luis J.
- Venue
- Behavioural Brain Research
- Publisher
- Elsevier BV
- Published
- 2012-07
- Volume
- 232
- Issue
- 2
- Pages
- 400-405
- ISSN
- 0166-4328 (ISSN-L)
- OpenAlex
- W2151082833
- Cited by
- 50
- References
- 35
- Citations by year
- 2026: 3 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 9 · 2019: 2 · 2018: 6 · 2017: 3 · 2016: 1 · 2015: 8 · 2014: 2 · 2013: 7 · 2012: 1
- FWCI
- 2.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Behavioural Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory Processes and Influences
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- LPA1 receptor, c-Fos activation, hippocampus, episodic-like memory, novelty recognition, object recognition, CA1, medial prefrontal cortex, memory demand
- MeSH terms
- Animals, Exploratory Behavior, Hippocampus, Male, Mice, Inbred C57BL, Tissue Distribution, Proto-Oncogene Proteins c-fos, Mice, Knockout, Recognition, Psychology, Receptors, Lysophosphatidic Acid, Mice, Memory, Episodic
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