Heterogeneity within and between primary colorectal carcinomas and matched metastases as revealed by analysis of Ki-ras and p53 mutations

Albanese, Ida; Scibetta, Angelo Giuseppe; Migliavacca, Manuela; Russo, Antonio; Bazan, Viviana; Tomasino, Rosa Maria; Colomba, Paolo; Tagliavia, Marcello; La Farina, Mario

doi:10.1016/j.bbrc.2004.10.111

Cited by 91Genetic factors in colorectal cancerColorectal Cancer Treatments and StudiesCancer Genomics and Diagnostics

Heterogeneity within and between primary colorectal carcinomas and matched metastases as revealed by analysis of Ki-ras and p53 mutations

Albanese, Ida, Scibetta, Angelo Giuseppe, Migliavacca, Manuela, Russo, Antonio, Bazan, Viviana, Tomasino, Rosa Maria, Colomba, Paolo, Tagliavia, Marcello +1 more·2004·Biochemical and Biophysical Research Communications

DOI 10.1016/j.bbrc.2004.10.111

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Abstract

Analysis of the genetic status of Ki-ras and p53 in primary colorectal carcinomas and matched colorectal liver metastasis from 30 patients reveals an overall heterogeneity both within and between the two tumoral tissues. Both genes were found mutated with a similar frequency in both tissues; however, identical mutations in primary tumor and matched metastasis were found less frequently in the case of the Ki-ras than the p53 gene. Only in three cases the same p53 and Ki-ras mutations found in the primary tumor were found also in the metastasis. In several metastatic specimens the DNA bearing a mutation detected also in the primary tumor appears significantly less abundant than the wild-type DNA. These data are discussed in the light of current models of primary tumor/metastasis relationships.

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Conversion from both wild-type to mutant and mutant to wild-type KRAS have been observed in metastatic lesions.
“conversion from both wild-type to mutant and mutant to wild-type KRAS have been observed in metastatic lesions [56]”
From The Role of KRAS Mutations in Colorectal Cancer: Biological Insights, Clinical Implications, and Future Therapeutic Perspectives (2025) · cites this paper as [56]

The hallmarks of cancer
Hanahan, D., & Weinberg, R. A. (2000) · Cell · doi:10.1016/s0092-8674(00)81683-9 · Cited by 29,219 · see citing works
Gene expression profiling predicts clinical outcome of breast cancer
van 't Veer, L. J., Dai, H., van de Vijver, M. J., He, Y. D., Hart, A. A. M., Mao, M., Peterse, H. L., van der Kooy, K., Marton, M. J., Witteveen, A. T., et al. (2002) · Nature · doi:10.1038/415530a · Cited by 9,536 · see citing works
Cancer genes and the pathways they control
Vogelstein, B., & Kinzler, K. W. (2004) · Nature Medicine · doi:10.1038/nm1087 · Cited by 4,245 · see citing works
A multigenic program mediating breast cancer metastasis to bone
Kang, Y., Siegel, P. M., Shu, W., Drobnjak, M., Käkönen, S.-M., Cordon‐Cardo, C., Guise, T. A., & Massagué, J. (2003) · Cancer Cell · doi:10.1016/s1535-6108(03)00132-6 · Cited by 2,562 · see citing works
A molecular signature of metastasis in primary solid tumors
Ramaswamy, S., Ross, K. N., Lander, E. S., & Golub, T. R. (2002) · Nature Genetics · doi:10.1038/ng1060 · Cited by 2,448 · see citing works
A Phosphatase Associated with Metastasis of Colorectal Cancer
Saha, S., Bardelli, A., Buckhaults, P., Velculescu, V. E., Rago, C., Croix, B. S., Romans, K. E., Choti, M. A., Christoph Lengauer, C., Kinzler, K. W., et al. (2001) · Science · doi:10.1126/science.1065817 · Cited by 648 · see citing works
From latent disseminated cells to overt metastasis: Genetic analysis of systemic breast cancer progression
Schmidt‐Kittler, O., Ragg, T., Daskalakis, A., Granzow, M., Ahr, A., Blankenstein, T. J. F., Kaufmann, M., Diebold, J., Arnholdt, H., Müller, P. E., et al. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1331931100 · Cited by 631 · see citing works
Mutations in APC, Kirsten-ras, and p53—alternative genetic pathways to colorectal cancer
Smith, G. C., Carey, F. A., Beattie, J., Wilkie, M. J. V., Lightfoot, T. J., Coxhead, J. M., Garner, R. C., Steele, R. J., & Wolf, C. R. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.122612899 · Cited by 520 · see citing works
Landscaping the Cancer Terrain
Kinzler, K. W., & Vogelstein, B. (1998) · Science · doi:10.1126/science.280.5366.1036 · Cited by 511 · see citing works
Know thy neighbor: stromal cells can contribute oncogenic signals
Tlsty, T. D., & Hein, P. W. (2001) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(00)00156-8 · Cited by 425 · see citing works
Regulation of mammary gland branching morphogenesis by the extracellular matrix and its remodeling enzymes
Fata, J. E., Werb, Z., & Bissell, M. J. (2003) · Breast Cancer Research · doi:10.1186/bcr634 · Cited by 387 · see citing works
Life (and death) in a malignant tumour
Kinzler, K. W., & Vogelstein, B. (1996) · Nature · doi:10.1038/379019a0 · Cited by 197 · see citing works
Hypermethylation of the APC (adenomatous Polyposis Coli) gene promoter region in human colorectal carcinoma
Hiltunen, M., Alhonen, L. I., Koıstınaho, J., Myöhänen, S., Pääkkönen, M. E., Marin, S., Kosma, V.-M., & Jänne, J. (1997) · International Journal of Cancer · doi:10.1002/(sici)1097-0215(19970317)70:6 · Cited by 192 · see citing works
Focus on colon cancer
Markowitz, S. D., Dawson, D. M., Willis, J. E., & Willson, J. K. V. (2002) · Cancer Cell · doi:10.1016/s1535-6108(02)00053-3 · Cited by 171 · see citing works
Concordant p53 and DCC alterations and allelic losses on chromosomes 13q and 14q associated with liver metastases of colorectal carcinoma
Ookawa, K., Sakamoto, M., Hirohashi, S., Yoshida, Y., Sügimura, T., Terada, M., & Yokota, J. (1993) · International Journal of Cancer · doi:10.1002/ijc.2910530307 · Cited by 134 · see citing works
Microvessel quantification and its possible relation with liver metastasis in colorectal cancer
Tomisaki, S., Ohno, S., Ichiyoshi, Y., Kuwano, H., Maehara, Y., & Sugimachi, K. (1996) · Cancer · doi:10.1002/(sici)1097-0142(19960415)77:8 · Cited by 118 · see citing works
Molecular abnormalities in liposarcoma: role of MDM2 and CDK4-containing amplicons at 12q13-22
Pilotti, S., Torre, G. D., Lavarino, C. E., Sozzi, G., Minoletti, F., Vergani, B., Azzarelli, A., Rilke, F. O., & Pierotti, M. A. (1998) · The Journal of Pathology · doi:10.1002/(sici)1096-9896(199806)185:2 · Cited by 98 · see citing works
Increased incidence of p53 mutations is associated with hepatic metastasis in colorectal neoplastic progression.
Kastrinakis, W. V., Ramchurren, N., Rieger, K. M., Hess, D. T., Loda, M. F., Steele, G., & Summerhayes, I. C. (1995) · PubMed · Cited by 78 · see citing works
p53 gene mutations are not required for early dissemination of cancer cells
Offner, S., Schmaus, W., Witter, K., Baretton, G. B., Schlimok, G., Passlick, B., Riethmüller, G., & Pantel, K. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.12.6942 · Cited by 50 · see citing works
Prognostic significance of proliferative activity, DNA-ploidy, p53 and Ki-ras point mutations in colorectal liver metastases
Russo, A. D., Migliavacca, M., Bazan, V., Maturi, N., Morello, V., Dardanoni, G., Modica, G., Bazan, P., Albanese, I., Farina, M. L., et al. (1998) · Cell Proliferation · doi:10.1046/j.1365-2184.1998.00116.x · Cited by 32 · see citing works
Self-citation
Prognostic significance of proliferative activity, DNA‐ploidy, p53 and Ki‐ras point mutations in colorectal liver metastases
Russo, A. D., Migliavacca, M., Bazan, V., et al. (1998) · Cell Proliferation · doi:10.1111/j.1365-2184.1998.tb01192.x · Cited by 22 · see citing works
Self-citation
Direct Identification of Each Specific Mutation in Codon 12 and 13 of ci-ki-ras2 by SSCP Analysis
Farina, M. L., Maturi, N., Stira, S., Russo, A. D., Bazan, V., & Albanese, I. (1998) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1998.8716 · Cited by 8 · see citing works
Self-citation

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ESMO consensus guidelines for the management of patients with metastatic colorectal cancer
Van Cutsem, E., Cervantes, A., Adam, R., Sobrero, A., Van Krieken, J. H., Aderka, D., Aranda Aguilar, E., Bardelli, A., Benson, A., Bodoky, G., et al. (2016) · Annals of Oncology · doi:10.1093/annonc/mdw235 · Cited by 3,421 · see citing works
Targeting metastasis
Steeg, P. S. (2016) · Nature Reviews Cancer · doi:10.1038/nrc.2016.25 · Cited by 1,363 · see citing works
Parallel progression of primary tumours and metastases
Klein, C. A. (2009) · Nature reviews. Cancer · doi:10.1038/nrc2627 · Cited by 1,124 · see citing works
Biomarkers Predicting Clinical Outcome of Epidermal Growth Factor Receptor–Targeted Therapy in Metastatic Colorectal Cancer
Siena, S., Bianchi, A. S., Nicolantonio, F. D., Balfour, J., & Bardelli, A. (2009) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/djp280 · Cited by 526 · see citing works
Prevalence and Heterogeneity of KRAS, BRAF, and PIK3CA Mutations in Primary Colorectal Adenocarcinomas and Their Corresponding Metastases
Baldus, S. E., Schaefer, K.-L., Engers, R., Hartleb, D., Stoecklein, N. H., & Gabbert, H. E. (2010) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-09-2446 · Cited by 432 · see citing works
Molecular epidemiology and diagnostics of KRAS mutations in human cancer
Timar, J., & Kashofer, K. (2020) · Cancer and Metastasis Reviews · doi:10.1007/s10555-020-09915-5 · Cited by 334 · see citing works
Metastasis: a therapeutic target for cancer
Steeg, P. S., & Theodorescu, D. (2008) · Nature Clinical Practice Oncology · doi:10.1038/ncponc1066 · Cited by 330 · see citing works
KRAS mutation analysis: A comparison between primary tumours and matched liver metastases in 305 colorectal cancer patients
Knijn, N., Mekenkamp, L. J. M., Klomp, M., Vink-Börger, M. E., Tol, J., Teerenstra, S., Meijer, J. W. R., Tebar, M., Riemersma, S., van Krieken, J. H. J. M., et al. (2011) · British Journal of Cancer · doi:10.1038/bjc.2011.26 · Cited by 291 · see citing works
Comparative Genomic Analysis of Primary Versus Metastatic Colorectal Carcinomas
Vakiani, E., Janakiraman, M., Shen, R., Sinha, R., Zeng, Z., Shia, J., Cercek, A., Kemeny, N. E., D'Angelica, M. I., Viale, A. J., et al. (2012) · Journal of Clinical Oncology · doi:10.1200/jco.2011.38.2994 · Cited by 282 · see citing works
Heterogeneity of Epidermal Growth Factor Receptor Status and Mutations of KRAS/PIK3CA in Circulating Tumor Cells of Patients with Colorectal Cancer
Gasch, C., Bauernhofer, T., Pichler, M., Langer-Freitag, S., Reeh, M., Seifert, A. M., Mauermann, O., Izbicki, J. R., Pantel, K., & Riethdorf, S. (2012) · Clinical Chemistry · doi:10.1373/clinchem.2012.188557 · Cited by 227 · see citing works
Comparison of epidermal growth factor receptor mutations between primary and corresponding metastatic tumors in tyrosine kinase inhibitor-naive non-small-cell lung cancer
Gow, C., Chang, Y., Hsu, Y.-C., Tsai, M.-F., Wu, C., Yu, C., Yang, J. C., Lee, Y., Yang, P., & Shih, J. (2008) · Annals of Oncology · doi:10.1093/annonc/mdn679 · Cited by 211 · see citing works
Genetic disparity between primary tumours, disseminated tumour cells, and manifest metastasis
Stoecklein, N. H., & Klein, C. A. (2009) · International Journal of Cancer · doi:10.1002/ijc.24916 · Cited by 186 · see citing works
Pragmatic issues in biomarker evaluation for targeted therapies in cancer
Gramont, A. D. E., Watson, S., Ellis, L. M., Rodon, J. A., Tabernero, J. M., de Gramont, A., & Hamilton, S. R. (2014) · Nature Reviews Clinical Oncology · doi:10.1038/nrclinonc.2014.202 · Cited by 174 · see citing works
KRAS and BRAF: drug targets and predictive biomarkers
Vakiani, E., & Solit, D. B. (2010) · The Journal of Pathology · doi:10.1002/path.2796 · Cited by 158 · see citing works
Increased Detection Sensitivity for KRAS Mutations Enhances the Prediction of Anti-EGFR Monoclonal Antibody Resistance in Metastatic Colorectal Cancer
Molinari, F., Felicioni, L., Buscarino, M., De Dosso, S., Buttitta, F., Malatesta, S., Movilia, A., Luoni, M., Boldorini, R., Alabiso, O., et al. (2011) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-10-3137 · Cited by 159 · see citing works
K-Ras Mutations and Treatment Outcome in Colorectal Cancer Patients Receiving Exclusive Fluoropyrimidine Therapy
Étienne-Grimaldi, M.-C., Formento, J., Francoual, M., François, É., Formento, P., Renée, N., Laurent‐Puig, P., Chazal, M., Benchimol, D., Delpéro, J., et al. (2008) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-07-4906 · Cited by 151 · see citing works
Different metastatic pattern according to the KRAS mutational status and site-specific discordance of KRAS status in patients with colorectal cancer
Kim, M. J., Lee, H. S., Kim, J. H., Kim, Y. J., Kwon, J. H., Lee, J., Bang, S., Park, K. U., Kim, D., Kang, S., et al. (2012) · BMC Cancer · doi:10.1186/1471-2407-12-347 · Cited by 141 · see citing works
Characterization of cancer genomic heterogeneity by next-generation sequencing advances precision medicine in cancer treatment
Zhang, J., Späth, S. S., Marjani, S. L., Zhang, W., & Pan, X. (2018) · Precision Clinical Medicine · doi:10.1093/pcmedi/pby007 · Cited by 128 · see citing works
Cancer metastasis: Molecular mechanisms and clinical perspectives
Khan, S. U., Fatima, K., Malik, F. A., Kalkavan, H., & Wani, A. (2023) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2023.108522 · Cited by 123 · see citing works
Comparative Genomic Analysis of Primary and Synchronous Metastatic Colorectal Cancers
Lee, S. Y., Haq, F. U., Kim, D., Jun, C., Jo, H.-J., Ahn, S., & Lee, W. (2014) · PLoS ONE · doi:10.1371/journal.pone.0090459 · Cited by 117 · see citing works
Biomarker concordance between primary colorectal cancer and its metastases
Bhullar, D., Barriuso, J., Mullamitha, S., Saunders, M. P., O’Dwyer, S., & Aziz, O. (2019) · EBioMedicine · doi:10.1016/j.ebiom.2019.01.050 · Cited by 115 · see citing works
Concordant analysis of KRAS, BRAF, PIK3CA mutations and PTEN expression between primary colorectal cancer and matched metastases
Mao, C., Wu, X.-Y., Yang, Z.-Y., Threapleton, D. E., Yuan, J.-Q., Yu, Y.-Y., & Tang, J.-L. (2015) · Scientific Reports · doi:10.1038/srep08065 · Cited by 107 · see citing works
Relationship between 18F-Fluorodeoxyglucose Accumulation and KRAS / BRAF Mutations in Colorectal Cancer
Kawada, K., Nakamoto, Y., Kawada, M., Hida, K., Matsumoto, T., Murakami, T., Hasegawa, S., Togashi, K., & Sakai, Y. (2012) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-11-1909 · Cited by 104 · see citing works
Cancer stem cells as a therapeutic target in bladder cancer
Aghaalikhani, N., Rashtchizadeh, N., Shadpour, P., Allameh, A., & Mahmoodi, M. (2018) · Journal of Cellular Physiology · doi:10.1002/jcp.26916 · Cited by 96 · see citing works
Pancreatic cancer: Circulating Tumor Cells and Primary Tumors show Heterogeneous KRAS Mutations
Kulemann, B., Rösch, S., Seifert, S., Timme, S., Bronsert, P., Seifert, G. J., Martini, V., Kuvendjiska, J., Glatz, T., Hussung, S., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-04601-z · Cited by 94 · see citing works
Concordance of predictive markers for EGFR inhibitors in primary tumors and metastases in colorectal cancer: A review
Baas, J. M., Krens, L. L., Guchelaar, H.-J., Morreau, H., & Gelderblom, H. (2011) · The Oncologist · doi:10.1634/theoncologist.2011-0024 · Cited by 89 · see citing works
KRAS Mutation Testing in Human Cancers: The Pathologist's Role in the Era of Personalized Medicine
Wang, H. L., Lopategui, J. R., Amin, M. B., & Patterson, S. D. (2010) · Advances in Anatomic Pathology · doi:10.1097/pap.0b013e3181c6962f · Cited by 88 · see citing works
Tumor Heterogeneity in Primary Colorectal Cancer and Corresponding Metastases. Does the Apple Fall Far From the Tree?
Blank, A., ROBERTS, D. E., Dawson, H., Zlobec, I., & Lugli, A. (2018) · Frontiers in Medicine · doi:10.3389/fmed.2018.00234 · Cited by 87 · see citing works
Cancer Stem Cells in Tumor Heterogeneity
Pietras, A. (2011) · Advances in cancer research · doi:10.1016/b978-0-12-387688-1.00009-0 · Cited by 83 · see citing works
The Role of KRAS Mutations in Colorectal Cancer: Biological Insights, Clinical Implications, and Future Therapeutic Perspectives
Takeda, M., Yoshida, S., Inoue, T., Sekido, Y., Hata, T., Hamabe, A., Ogino, T., Miyoshi, N., Uemura, M., Yamamoto, H., et al. (2025) · Cancers · doi:10.3390/cancers17030428 · Cited by 82 · see citing works
A target selection of somatic hypermutations is regulated similarly between T and B cells upon activation-induced cytidine deaminase expression
Kotani, A., Okazaki, I., Muramatsu, M., Kinoshita, K., Begum, N. A., Nakajima, T., Saito, H., & Honjo, T. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0500830102 · Cited by 75 · see citing works
Regulation of 18F-FDG Accumulation in Colorectal Cancer Cells with Mutated KRAS
Iwamoto, M., Kawada, K., Nakamoto, Y., Itatani, Y., Inamoto, S., Toda, K., Kimura, H., Sasazuki, T., Shirasawa, S., Okuyama, H., et al. (2014) · Journal of Nuclear Medicine · doi:10.2967/jnumed.114.142927 · Cited by 70 · see citing works
Pancreatic Cancer Metastasis: Are we being Pre-EMTed?
Das, S., & Batra, S. K. (2015) · Current Pharmaceutical Design · doi:10.2174/1381612821666141211115234 · Cited by 65 · see citing works
18F-FDG PET/CT imaging in rectal cancer: relationship with theRASmutational status
Lovinfosse, P., Koopmansch, B., Lambert, F., Jodogne, S., Kustermans, G., Hatt, M., Visvikis, D., Seidel, L., Polus, M., Albert, A., et al. (2016) · British Journal of Radiology · doi:10.1259/bjr.20160212 · Cited by 63 · see citing works
Relationship Between 18F-FDG PET/CT Scans and KRAS Mutations in Metastatic Colorectal Cancer
Kawada, K., Toda, K., Nakamoto, Y., Iwamoto, M., Hatano, E., Chen, F., Hasegawa, S., Togashi, K., Date, H., Üemoto, S., et al. (2015) · Journal of Nuclear Medicine · doi:10.2967/jnumed.115.160614 · Cited by 58 · see citing works
Alterations in the EGFR pathway coincide in colorectal cancer and impact on prognosis
Neumann, J. H. L., Wehweck, L., Maatz, S., Engel, J., Kirchner, T., & Jung, A. (2013) · Archiv für Pathologische Anatomie und Physiologie und für Klinische Medicin · doi:10.1007/s00428-013-1450-0 · Cited by 56 · see citing works
Comparison of p53 and Epidermal Growth Factor Receptor Gene Status Between Primary Tumors and Lymph Node Metastases in Non-Small Cell Lung Cancers
Chang, Y., Wu, C., Shih, J., & Lee, Y.-C. (2010) · Annals of Surgical Oncology · doi:10.1245/s10434-010-1295-6 · Cited by 56 · see citing works
c-MYC Copy-Number Gain Is an Independent Prognostic Factor in Patients with Colorectal Cancer
Lee, K. S., Kwak, Y., Nam, K. H., Kim, D., Kang, S., Choe, G., Kim, W. H., & Lee, H. S. (2015) · PLoS ONE · doi:10.1371/journal.pone.0139727 · Cited by 55 · see citing works
Prognostic Value of Circulating Mutant DNA in Unresectable Metastatic Colorectal Cancer
Lefébure, B., Charbonnier, F., Fiore, F. D., Tuech, J., Pessot, F. L., Michot, F., Michel, P., & Frébourg, T. (2010) · Annals of Surgery · doi:10.1097/sla.0b013e3181c35c87 · Cited by 53 · see citing works
Mechanisms underlying18F-fluorodeoxyglucose accumulation in colorectal cancer
Kawada, K., Iwamoto, M., & Sakai, Y. (2016) · World Journal of Radiology · doi:10.4329/wjr.v8.i11.880 · Cited by 54 · see citing works
Zinc induced apoptosis in HEP-2 cancer cells: The role of oxidative stress and mitochondria
Rudolf, E., Rudolf, K., & Červinka, M. (2005) · BioFactors · doi:10.1002/biof.5520230206 · Cited by 53 · see citing works
Management of resectable colorectal lung metastases
Moorcraft, S. Y., Ladas, G., Bowcock, A. M., & Chau, I. (2015) · Clinical & Experimental Metastasis · doi:10.1007/s10585-015-9774-6 · Cited by 50 · see citing works
Methylation levels of LINE-1 in primary lesion and matched metastatic lesions of colorectal cancer
Murata, A., Baba, Y., Watanabe, M., Shigaki, H., Miyake, K., Ishimoto, T., Iwatsuki, M., Iwagami, S., Sakamoto, Y., Miyamoto, Y., et al. (2013) · British Journal of Cancer · doi:10.1038/bjc.2013.289 · Cited by 47 · see citing works
Favorable prognosis in colorectal cancer patients with co-expression of c-MYC and ß-catenin
Lee, K. S., Kwak, Y., Nam, K. H., Kim, D., Kang, S., Choe, G., Kim, W. H., & Lee, H. S. (2016) · BMC Cancer · doi:10.1186/s12885-016-2770-7 · Cited by 47 · see citing works
Coexistence of KRAS and BRAF Mutations in Colorectal Cancer: A Case Report Supporting The Concept of Tumoral Heterogeneity.
Larki, P., Gharib, E., Taleghani, M. Y., Khorshidi, F., Mojarad, E. N., & Asadzadeh Aghdaei, H. (2017) · Europe PMC (PubMed Central) · doi:10.22074/cellj.2017.5123 · Cited by 44 · see citing works
Mutations in TP53 are a prognostic factor in colorectal hepatic metastases undergoing surgical resection
Mollevı́, D. G., Serrano, T., Ginesta, M. M., Valls, J., Torrás, J., Navarro, M., Ramos, E., Germà, J. R., Jaurrieta, E., Moreno, V. R., et al. (2007) · Carcinogenesis · doi:10.1093/carcin/bgm012 · Cited by 41 · see citing works
RAS Mutations as Predictive Biomarkers in Clinical Management of Metastatic Colorectal Cancer
Waring, P. M., Tie, J., Maru, D. M., & Karapetis, C. S. (2015) · Clinical Colorectal Cancer · doi:10.1016/j.clcc.2015.10.006 · Cited by 38 · see citing works
Concordant analysis of KRAS status in primary colon carcinoma and matched metastasis.
Mariani, P., Lae, M. E., Degeorges, A., Cacheux, W., Lappartient, E., Margogne, A., Pierga, J., Girre, V., Mignot, L., Falcou, M. C., et al. (2010) · PubMed · Cited by 37 · see citing works
KRAS testing in metastatic colorectal carcinoma: challenges, controversies, breakthroughs and beyond
Malapelle, U., Carlomagno, C., Luca, C. D., Bellevicine, C., & Troncone, G. (2013) · Journal of Clinical Pathology · doi:10.1136/jclinpath-2013-201835 · Cited by 34 · see citing works
Analysis of KIT expression and gene mutation in human acral melanoma: with a comparison between primary tumors and corresponding metastases/recurrences
Dai, B., Xu, C., Kong, Y.-Y., Yang, F., Shen, X.-X., Wang, L.-W., & Kong, J.-C. (2013) · Human Pathology · doi:10.1016/j.humpath.2013.01.007 · Cited by 34 · see citing works
Current therapy of advanced colorectal cancer according to RAS/RAF mutational status
Lakatos, G., Köhne, C., & Bodoky, G. (2020) · Cancer and Metastasis Reviews · doi:10.1007/s10555-020-09913-7 · Cited by 30 · see citing works
KRAS mutation testing in colorectal cancer as an example of the pathologist’s role in personalized targeted therapy: a practical approach
Domagała, P., Hybiak, J., Sulżyc‐Bielicka, V., Cybulski, C., Ryś, J., & Domagała, W. (2012) · Polish Journal of Pathology · doi:10.5114/pjp.2012.31499 · Cited by 31 · see citing works
The Clinical Implication of Cancer-Associated Microvasculature and Fibroblast in Advanced Colorectal Cancer Patients with Synchronous or Metachronous Metastases
Kwak, Y., Lee, H. E., Kim, W. H., Kim, D., Kang, S.-B., & Lee, H. S. (2014) · PLoS ONE · doi:10.1371/journal.pone.0091811 · Cited by 29 · see citing works
Tumor evolution and intratumor heterogeneity in colorectal carcinoma: insights from comparative genomic profiling of primary tumors and matched metastases.
Sylvester, B. E., & Vakiani, E. (2015) · Europe PMC (PubMed Central) · doi:10.3978/j.issn.2078-6891.2015.083 · Cited by 29 · see citing works
O6-Methylguanine-DNA methyltransferase expression and prognostic value in brain metastases of lung cancers
Wu, P.-F., Kuo, K., Kuo, L., Lin, Y., Lee, W.-C., Lu, Y., Yang, C.-H., Wu, R., Tu, Y., Tasi, J.-C., et al. (2009) · Lung Cancer · doi:10.1016/j.lungcan.2009.08.010 · Cited by 28 · see citing works
Expression of HGF and Met in Human Tissues of Colorectal Cancers: Biological and Clinical Implications for Synchronous Liver Metastasis
Sun, Y., Liu, W., Ma, G., Gao, D.-W., Jiang, Y., Liu, Q., & Du, J. (2013) · International Journal of Medical Sciences · doi:10.7150/ijms.5191 · Cited by 24 · see citing works
Prognostic value of KRAS genotype in metastatic colorectal cancer (MCRC) patients treated with intensive triplet chemotherapy plus bevacizumab (FIr-B/FOx) according to extension of metastatic disease
Bruera, G., Cannita, K., Giacomo, D. D., Lamy, A., Troncone, G., Mas, A. D., Coletti, G., Frébourg, T., Sabourin, J. C., Tosi, M., et al. (2012) · BMC Medicine · doi:10.1186/1741-7015-10-135 · Cited by 23 · see citing works
Evidence of intermetastatic heterogeneity for pathological response and genetic mutations within colorectal liver metastases following preoperative chemotherapy
Sebagh, M., Allard, M., Bosselut, N., Dao, M., Vibert, É., Lewin, M., Lemoine, A., Cherqui, D., Adam, R. A., & Cunha, A. S. (2016) · Oncotarget · doi:10.18632/oncotarget.7809 · Cited by 23 · see citing works
Engineering Multidimensional Evolutionary Forces to Combat Cancer
McCoach, C. E., & Bivona, T. G. (2019) · Cancer Discovery · doi:10.1158/2159-8290.cd-18-1196 · Cited by 22 · see citing works
Liver Metastasis in Cancer: Molecular Mechanisms and Management
Xu, W.-C., Xu, J., Liu, J.-Z., Wang, N., Zhou, L., & Guo, J.-C. (2025) · MedComm · doi:10.1002/mco2.70119 · Cited by 24 · see citing works
Stromal Expression of MicroRNA-21 in Advanced Colorectal Cancer Patients with Distant Metastases
Lee, K. S., Nam, S. K., Koh, J., Kim, D., Kang, S., Choe, G., Kim, W. H., & Lee, H. S. (2016) · Journal of Pathology and Translational Medicine · doi:10.4132/jptm.2016.03.19 · Cited by 21 · see citing works
Carrier molecules and extraction of circulating tumor DNA for next generation sequencing in colorectal cancer
Beránek, M., Sirák, I., Vošmik, M., Petera, J., Drastíková, M., & Palička, V. (2016) · Acta Medica (Hradec Kralove Czech Republic) · doi:10.14712/18059694.2016.54 · Cited by 21 · see citing works
Spatio-temporal tumor heterogeneity in metastatic CRC tumors: a mutational-based approach
del Carmen, S., Sayagués, J. M., Bengoechea, Ó., Anduaga, M. F., Alcázar, J. A. S., Gervás, R., García, J., Órfão, A., Muñoz‐Bellvís, L., Sarasquete, M. E., et al. (2018) · Oncotarget · doi:10.18632/oncotarget.26081 · Cited by 21 · see citing works
Germline p53 single-base changes associated with Balkan endemic nephropathy
Krasteva, M. E., & Georgieva, E. (2006) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2006.02.004 · Cited by 19 · see citing works
KRAS discordance between primary and recurrent tumors after radical resection of colorectal cancers
Lee, K. H., Kim, J. S., Lee, C. S., & Kim, J. (2015) · Journal of Surgical Oncology · doi:10.1002/jso.23936 · Cited by 16 · see citing works
Comparison of KRAS mutation status between primary tumor and metastasis in Chinese colorectal cancer patients
Li, Z.-Z., Bai, L., Wang, F., Zhang, Z.-C., Wang, F., Zeng, Z.-L., Zeng, J., Zhang, D.-S., Wang, F., Wang, Z., et al. (2016) · Medical Oncology · doi:10.1007/s12032-016-0787-z · Cited by 16 · see citing works
Role of Src in breast cancer cell migration and invasion in a breast cell/bone-derived cell microenvironment
Pohorelic, B. K. J., Singh, R., Parkin, S., Koro, K., Yang, A.-D., Egan, C., & Magliocco, A. M. (2011) · Breast Cancer Research and Treatment · doi:10.1007/s10549-011-1753-2 · Cited by 16 · see citing works
Decrease of survivin, p53 and Bcl-2 expression in chemorefractory colorectal liver metastases may be predictive of radiosensivity after radioembolization with yttrium-90 resin microspheres
Melucci, E., Cosimelli, M., Carpanese, L., Pizzi, G., Izzo, F., Fiore, F., Golfieri, R., Giampalma, E., Sperduti, I., Ercolani, C., et al. (2013) · Journal of Experimental & Clinical Cancer Research · doi:10.1186/1756-9966-32-13 · Cited by 16 · see citing works
Delineation of the infrequent mosaicism of KRAS mutational status in metastatic colorectal adenocarcinomas
Bossard, C., Küry, S., Jamet, P., Senellart, H., Airaud, F., Ramée, J., Bezieau, S., Matysiak‐Budnik, T., LABOISSE, C. L., & Mosnier, J. (2012) · Journal of Clinical Pathology · doi:10.1136/jclinpath-2011-200608 · Cited by 15 · see citing works
Heterogeneity analysis of 18F-FDG PET imaging in oncology: clinical indications and perspectives
Lovinfosse, P., Hatt, M., Visvikis, D., & Hustinx, R. (2018) · Clinical and Translational Imaging · doi:10.1007/s40336-018-0299-2 · Cited by 15 · see citing works
Degraded DNA may induce discordance of KRAS status between primary colorectal cancer and corresponding liver metastases
Kaneko, Y., Kuramochi, H., Nakajima, G., Inoue, Y., & Yamamoto, M. (2013) · International Journal of Clinical Oncology · doi:10.1007/s10147-012-0507-4 · Cited by 14 · see citing works
Coexistence of two different mutations in codon 12 of the Kras gene in colorectal cancer: Report of a case supporting the concept of tumoral heterogeneity
Improta, G., Zupa, A., Possidente, L., Tartarone, A., Pedicini, P., Nappi, A., MOLINARI, S., Fraggetta, F., & Vita, G. (2013) · Oncology Letters · doi:10.3892/ol.2013.1255 · Cited by 12 · see citing works
Comparison of KRAS/BRAF mutations between primary tumors and serum in colorectal cancer: Biological and clinical implications
Pu, X., Pan, Z., Huang, Y., TIAN, Y., Guo, H., Wu, L., HE, X., Chen, X., Zhang, S., & Lin, T. (2012) · Oncology Letters · doi:10.3892/ol.2012.963 · Cited by 12 · see citing works
Putative precursor cancer cells in human colorectal cancer tissue.
Goranova, T., Ohue, M., & Kato, K. (2009) · PubMed Central · Cited by 9 · see citing works
Metastatic disease: A drug discovery perspective
Lehembre, F., & Regenass, U. (2012) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2012.03.005 · Cited by 8 · see citing works
Industasis, a Promotion of Tumor Formation by Nontumorigenic Stray Cells
Pajer, P., Karafiát, V., Pečenka, V., Průková, D., Dudlová, J., Plachý, J., Kašparová, P., & Dvořák, M. (2009) · Cancer Research · doi:10.1158/0008-5472.can-08-4636 · Cited by 7 · see citing works
Investigating genomic, proteomic, and post-transcriptional regulation profiles in colorectal cancer: a comparative study between primary tumors and associated metastases
Ham-Karim, H. A., Negm, O. H., Ahmad, N., & Ilyas, M. (2023) · Cancer Cell International · doi:10.1186/s12935-023-03020-7 · Cited by 6 · see citing works
Gene expression differences in primary colorectal tumors and matched liver metastases: chemotherapy related or tumoral heterogeneity?
López-Gómez, M., Moreno‐Rubio, J., Suárez‐García, I., Cejas, P., Madero, R., Casado, E., Jiménez, A. M., Sereno, M., Gómez-Raposo, C., Zambrana, F. R., et al. (2014) · Clinical & Translational Oncology · doi:10.1007/s12094-014-1233-3 · Cited by 5 · see citing works
Multiplexed Dosing Assays by Digitally Definable Hydrogel Volumes
Faralli, A., Melander, F., Larsen, E., Chernyy, S., Andresen, T. L., & Larsen, N. B. (2015) · Advanced Healthcare Materials · doi:10.1002/adhm.201500542 · Cited by 4 · see citing works
Association of TP53 gene polymorphisms with the risk of acute lymphoblastic leukemia in Moroccan children
Skhoun, H., Khattab, M., Belkhayat, A., Chebihi, Z. T., Bakri, Y., Dakka, N., & Baghdadi, J. E. (2022) · Molecular Biology Reports · doi:10.1007/s11033-022-07643-3 · Cited by 4 · see citing works
Molecular Testing of Colorectal Cancer in the Modern Era
Vakiani, E. (2017) · Surgical pathology clinics · doi:10.1016/j.path.2017.07.013 · Cited by 4 · see citing works
Breast Cancer Metastasis: Advances Through the Use of In Vitro Co-Culture Model Systems
Magliocco, A. M., & Eg, C. (2011) · InTech eBooks · doi:10.5772/21743 · Cited by 4 · see citing works
Biobanking metastases and biopsy specimens for personalized medicine
Liu, A., Collins, C., & Diemer, S. (2015) · Journal of Biorepository Science for Applied Medicine · doi:10.2147/bsam.s64382 · Cited by 4 · see citing works
Meta-analysis of clinical trials on concordance of mutational stauts of primary tumour and distant metastases of colorectal cancer
Fedyanin, M. Y., Tryakin, A. A., Покатаев, И. А., & Tyulyandin, S. А. (2017) · Surgery and Oncology · doi:10.17650/2220-3478-2017-7-1-27-41 · Cited by 3 · see citing works
Diagnosis of primary versus metastatic ovarian adenocarcinoma using p53 gene mutation analysis
Yamano, T., Morii, E., Arai, I., Takada, T., Kubota, K., Sato, M., Inoue, T., Okada, Y., Hara, T., & Aozasa, K. (2010) · International Journal of Clinical Oncology · doi:10.1007/s10147-010-0096-z · Cited by 2 · see citing works
Benefit of rebiopsy for deciding treatment strategy in rectal cancer: A case report
Kawasaki, K., Hamamoto, Y., Suzuki, T., Hirata, K., Sukawa, Y., Kasuga, A., Hayashi, Y., Takaishi, H., Kameyama, K., & Kanai∥, T. (2017) · Oncology Letters · doi:10.3892/ol.2017.6601 · Cited by 1 · see citing works
Original Article Putative Precursor Cancer Cells in Human Colorectal Cancer Tissue
Goranova, T., Ohue, M., & Kato, K. (2008)
Marqueurs moléculaires prédictifs de réponse aux thérapies ciblées dans les cancers digestifs
Perkins, G. (2012) · theses.fr (ABES) · doi:10.70675/5b4631efzc0e5z4dddz8408z43ab410793f3
The role of KRAS and NRAS mutation detection in determining the therapy strategy for colorectal cancer
Toropovskiy, A. N., Nikitin, A. G., Викторов, Д. А., Solov'ev, A. V., Khuzina, R. M., & Pavlova, O. N. (2022) · Bulletin of the Medical Institute REAVIZ (REHABILITATION DOCTOR AND HEALTH) · doi:10.20340/vmi-rvz.2022.5.clin.8
Comparative Analysis of Primary Tumour and Matched Metastases in Colorectal Cancer Patients: An Investigation of the Genomic, Proteomic and Post-Transcriptional Regulation Profiles
Karim, H., Negm, O. H., Narmeen, A., & Ilyas, M. (2022) · International Journal of Cancer and Clinical Research · doi:10.23937/2378-3419/1410180
Critical Evaluation of Treatment Response, Driver Mutations, and Circulating Tumor DNA as Markers of Tumor Biology in Colorectal Liver Metastasis
Madi, M., Haddad, A., Ito, K., Bhutiani, N., & Vauthey, J. (2026) · Cancers · doi:10.3390/cancers18071111

Status of K-ras oncogene in primary tumors and related liver metastases in colorectal cancer
Shou-zh, L. (2011) · Journal of Digestive Oncology
Comparison of KRAS and PIK3CA gene status between primary tumors and paired metastases in colorectal cancer
He, Q., Ying, J., Xu, Q., Wu, W., Chen, L., & Sun, W. (2016) · OncoTargets and Therapy · doi:10.2147/ott.s97668 · Cited by 15 · see citing works
Comparative Genomic Analysis of Primary Versus Metastatic Colorectal Carcinomas
Vakiani, E., Janakiraman, M., Shen, R., Sinha, R., Zeng, Z., Shia, J., Cercek, A., Kemeny, N. E., D'Angelica, M. I., Viale, A. J., et al. (2012) · Journal of Clinical Oncology · doi:10.1200/jco.2011.38.2994 · Cited by 282 · see citing works
KRAS mutation analysis: A comparison between primary tumours and matched liver metastases in 305 colorectal cancer patients
Knijn, N., Mekenkamp, L. J. M., Klomp, M., Vink-Börger, M. E., Tol, J., Teerenstra, S., Meijer, J. W. R., Tebar, M., Riemersma, S., van Krieken, J. H. J. M., et al. (2011) · British Journal of Cancer · doi:10.1038/bjc.2011.26 · Cited by 291 · see citing works
P-352 Comparison of KRAS status between primary colorectal tumor and metastasis or recurrent tumor
Douik, H., Sahraoui, G., Doghri, R., Charfi, L., Hajer, B., & Mrad, K. (2023) · Annals of Oncology · doi:10.1016/j.annonc.2023.04.408

Authors: Albanese, I., Scibetta, A. G., Migliavacca, M., Russo, A., Bazan, V., Tomasino, R. M., Colomba, P., Tagliavia, M., & La Farina, M.
Affiliations: University of Palermo (IT)
Guy's Hospital (GB)
ORCID: Russo, Antonio
Bazan, Viviana
Colomba, Paolo
Tagliavia, Marcello
Venue: Biochemical and Biophysical Research Communications
Publisher: Elsevier BV
Published: 2004-12
Volume: 325
Issue: 3
Pages: 784-791
ISSN: 0006-291X (ISSN-L)
DOI: 10.1016/j.bbrc.2004.10.111
OpenAlex: W1967325169
Cited by: 91
References: 22
Citations by year: 2026: 1 · 2025: 2 · 2023: 2 · 2022: 3 · 2020: 2 · 2019: 2 · 2018: 5 · 2017: 5 · 2016: 9 · 2015: 10 · 2014: 5 · 2013: 8 · 2012: 10
FWCI: 0.91
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Biochemical and Biophysical Research Communications (publishedVersion)
PubMed (publishedVersion)
Nova Science Publishers (Nova Science Publishers, Inc.) (submittedVersion)
Funders: Ministero dell’Istruzione, dell’Università e della Ricerca
Topics: Health Sciences › Medicine › Pathology and Forensic Medicine › Genetic factors in colorectal cancer
Health Sciences › Medicine › Oncology › Colorectal Cancer Treatments and Studies
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
Keywords: colorectal cancer, colorectal liver metastases, Ki-ras mutation, TP53 mutation, tumor heterogeneity
MeSH terms: Carcinoma, DNA Mutational Analysis, Humans, Liver Neoplasms, Mutation, Genes, ras, Sequence Homology, Nucleic Acid, Statistics as Topic, Colorectal Neoplasms, Oncogene Protein p21(ras), Gene Expression Regulation, Neoplastic, Tumor Suppressor Protein p53, Sequence Analysis, DNA, Gene Expression Profiling

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Heterogeneity within and between primary colorectal carcinomas and matched metastases as revealed by analysis of Ki-ras and p53 mutations

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