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Cited by 67Tissue Engineering and Regenerative MedicineCellular Mechanics and Interactions3D Printing in Biomedical Research

Three-dimensional decellularized tumor extracellular matrices with different stiffness as bioengineered tumor scaffolds

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Lv, Yonggang, Wang, Hongjun, Li, Gui, Zhao, Boyuan·2021·Bioactive Materials
DOI 10.1016/j.bioactmat.2021.02.004PMC PMC7897907
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Abstract

tumor models with appropriate matrix stiffness are urgently desired. Herein, we prepare 3D decellularized extracellular matrix (DECM) scaffolds with different stiffness to mimic the microenvironment of human breast tumor tissue, especially the matrix stiffness, components and structure of ECM. Furthermore, the effects of matrix stiffness on the drug resistance of human breast cancer cells are explored with these developed scaffolds as case studies. Our results confirm that DECM scaffolds with diverse stiffness can be generated by tumor cells with different lysyl oxidase (LOX) expression levels, while the barely intact structure and major components of the ECM are maintained without cells. This versatile 3D tumor model with suitable stiffness can be used as a bioengineered tumor scaffold to investigate the role of the microenvironment in tumor progression and to screen drugs prior to clinical use to a certain extent.

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Authors
Lv, Y., Wang, H., Li, G., & Zhao, B.
Affiliations
Chongqing University (CN)
Corresponding author
Lv, Yonggang
ORCID
Venue
Bioactive Materials
Publisher
Elsevier BV
Published
2021-09
Volume
6
Issue
9
Pages
2767-2782
ISSN
  • 2097-1192 (ISSN-L)
  • 2452-199X
DOI
10.1016/j.bioactmat.2021.02.004
PMC
PMC7897907
OpenAlex
W3134029010
Cited by
67
References
83
Citations by year
2026: 5 · 2025: 10 · 2024: 17 · 2023: 15 · 2022: 14 · 2021: 6
FWCI
12.22
Type
journal article
Language
en
Open access
Licence
Locations
  • Bioactive Materials (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Natural Science Foundation of Chongqing (cstc2020jcyj-msxmX0035)
  • Natural Science Foundation Project of Chongqing
  • National Natural Science Foundation of China (12072054, 11872134)
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Keywords
decellularized extracellular matrix, extracellular matrix stiffness, breast cancer, 3D tumor models, lysyl oxidase, tumor microenvironment, drug resistance, drug screening, extracellular matrix components

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