Composite bioink incorporating cell-laden liver decellularized extracellular matrix for bioprinting of scaffolds for bone tissue engineering
Open accessAbstract
The field of bone tissue engineering (BTE) has witnessed a revolutionary breakthrough with the advent of three-dimensional (3D) bioprinting technology, which is considered an ideal choice for constructing scaffolds for bone regeneration. The key to realizing scaffold biofunctions is the selection and design of an appropriate bioink, and existing bioinks have significant limitations. In this study, a composite bioink based on natural polymers (gelatin and alginate) and liver decellularized extracellular matrix (LdECM) was developed and used to fabricate scaffolds for BTE using 3D bioprinting. Through in vitro studies, the concentration of LdECM incorporated into the bioink was optimized to achieve printability and stability and to improve the proliferation and osteogenic differentiation of loaded rat bone mesenchymal stem cells (rBMSCs). Furthermore, in vivo experiments were conducted using a Sprague Dawley rat model of critical-sized calvarial defects. The proposed rBMSC-laden LdECM-gelatin-alginate scaffold, bioprinted layer-by-layer, was implanted in the rat calvarial defect and the development of new bone growth was studied for four weeks. The findings showed that the proposed bioactive scaffolds facilitated angiogenesis and osteogenesis at the defect site. The findings of this study suggest that the developed rBMSC-laden LdECM-gelatin-alginate bioink has great potential for clinical translation and application in solving bone regeneration problems.
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Porcine liver was used in approximately 51 studies.
“The papers mentioned indicate that porcine liver has been the prominent material utilized in the creation of decellularized liver extracellular matrix (dLECM) that was utilized in approximately 51 studies.”
Significant variability in residual DNA content, ranging from below 50 ng/mg safety limit to levels substantially in excess, presents a direct risk of causing adverse host immune reactions.
“The significant variability in residual DNA content that is reported, ranging from below the 50 ng/mg safety limit through to levels substantially in excess, presents a direct risk of causing adverse host immune reactions [19,80].”
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- Authors
- You, P., Sun, H., Chen, H., Li, C., Mao, Y., Zhang, T., Yang, H., & Dong, H.
- Affiliations
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Capital Medical University (CN)
- Corresponding author
- Zhang, Tao
- Yang, Huayu
- Dong, Haitao
- Venue
- Biomaterials Advances
- Publisher
- Elsevier BV
- Published
- 2024-12
- Volume
- 165
- Pages
- 214017
- ISSN
- 2772-9508 (ISSN-L)
- OpenAlex
- W4402037439
- Cited by
- 14
- References
- 75
- Citations by year
- 2026: 6 · 2025: 8
- FWCI
- 1.54
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Biomaterials Advances (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- PUMCH
- CAMS
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- gelatin, alginate, 3D bioprinting, bone tissue engineering, bone regeneration, bone mesenchymal stem cells, osteogenic differentiation, calvarial defect, angiogenesis, bone formation, natural polymers
- MeSH terms
- Decellularized Extracellular Matrix, Alginates, Animals, Bone and Bones, Bone Regeneration, Cell Differentiation, Extracellular Matrix, Gelatin, Ink, Liver, Osteogenesis, Rats, Sprague-Dawley, Tissue Engineering, Cell Proliferation, Rats, Tissue Scaffolds, Mesenchymal Stem Cells, Bioprinting, Printing, Three-Dimensional
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