Cited by 222Bone Tissue Engineering MaterialsTissue Engineering and Regenerative MedicineCollagen: Extraction and Characterization

The healing of bony defects by cell-free collagen-based scaffolds compared to stem cell-seeded tissue engineered constructs

Lyons, Frank G., Al-Munajjed, Amir A., Kieran, Stephen M., Toner, Mary E., Murphy, Ciara M., Duffy, Garry P., O’Brien, Fergal J.·2010·Biomaterials

Abstract

One of the key challenges in tissue engineering is to understand the host response to scaffolds and engineered constructs. We present a study in which two collagen-based scaffolds developed for bone repair: a collagen-glycosaminoglycan (CG) and biomimetic collagen-calcium phosphate (CCP) scaffold, are evaluated in rat cranial defects, both cell-free and when cultured with MSCs prior to implantation. The results demonstrate that both cell-free scaffolds showed excellent healing relative to the empty defect controls and somewhat surprisingly, to the tissue engineered (MSC-seeded) constructs. Immunological analysis of the healing response showed higher M1 macrophage activity in the cell-seeded scaffolds. However, when the M2 macrophage response was analysed, both groups (MSC-seeded and non-seeded scaffolds) showed significant activity of these cells which are associated with an immunomodulatory and tissue remodelling response. Interestingly, the location of this response was confined to the construct periphery, where a capsule had formed, in the MSC-seeded groups as opposed to areas of new bone formation in the non-seeded groups. This suggests that matrix deposited by MSCs during in vitro culture may adversely affect healing by acting as a barrier to macrophage-led remodelling when implanted in vivo. This study thus improves our understanding of host response in bone tissue engineering.

Integrity signals

Registry

Crossref · 10.1016/j.biomaterials.2010.08.056

Cites

55 of 55 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Marked differences in M1 and M2 presence are observed 4 and 8 weeks after implantation of cell-free and MSC-filled porous collagen-based scaffolds.

    “Research from our group suggests that marked differences in M1 and M2 presence is observed 4 and 8 weeks after implantation of cell free and MSC filled porous collagen-based scaffolds (Fig. 6a,b,e,f) [82]”

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Lyons, F. G., Al-Munajjed, A. A., Kieran, S. M., Toner, M. E., Murphy, C. M., Duffy, G. P., & O’Brien, F. J.
Affiliations
  • Royal College of Surgeons in Ireland (IE)
  • Trinity College Dublin (IE)
Corresponding author
O’Brien, Fergal J.
Venue
Biomaterials
Publisher
Elsevier BV
Published
2010-12
Volume
31
Issue
35
Pages
9232-9243
ISSN
0142-9612 (ISSN-L)
OpenAlex
W2060078219
Cited by
222
References
54
Citations by year
2026: 5 · 2025: 5 · 2024: 3 · 2023: 11 · 2022: 17 · 2021: 14 · 2020: 11 · 2019: 24 · 2018: 18 · 2017: 15 · 2016: 15 · 2015: 20 · 2014: 20 · 2013: 18 · 2012: 12
FWCI
8.12
Type
journal article
Language
en
Open access
closed
Locations
  • Biomaterials (publishedVersion)
  • PubMed (publishedVersion)
  • Lenus, The Irish Health Repository (Dr Steevens Hospital Library) (submittedVersion)
Funders
  • Enterprise Ireland
  • Science Foundation Ireland (04/Yl1/B531, 06/RFP/ENM012)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
Keywords
bone defect repair, collagen scaffolds, cell-free scaffolds, mesenchymal stem cells, cranial defects, macrophage polarization, M1 macrophages, M2 macrophages, collagen-glycosaminoglycan scaffolds, bone tissue engineering, capsule formation, bone remodeling
MeSH terms
Animals, Biomechanical Phenomena, Bone Regeneration, Calcium Phosphates, Collagen, Glycosaminoglycans, Macrophages, Male, Wound Healing, Rats, Wistar, Tissue Engineering, Rats, Tissue Scaffolds, X-Ray Microtomography, Mesenchymal Stem Cells

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review