The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Hydrogels with time-dependent material properties enhance cardiomyocyte differentiation in vitro
Open accessAbstract
Tissue-specific elastic modulus (E), or ‘stiffness,’ arises from developmental changes in the extracellular matrix (ECM) and suggests that progenitor cell differentiation may be optimal when physical conditions mimic tissue progression. For cardiomyocytes, maturing from mesoderm to adult myocardium results in a 9-fold stiffening originating in part from a change in collagen expression and localization. To mimic this temporal stiffness change in vitro, thiolated-hyaluronic acid hydrogels were crosslinked with poly(ethylene glycol) diacrylate, and their dynamics were modulated by changing crosslinker molecular weight. With the hydrogel appropriately tuned to stiffen as heart muscle does during development, pre-cardiac cells grown on collagen-coated HA hydrogels exhibit a 3-fold increase in mature cardiac-specific markers and form up to 60% more maturing muscle fibers than they do when grown on compliant but static polyacrylamide hydrogels over 2 weeks. Though ester hydrolysis does not substantially alter hydrogel stiffening over 2 weeks in vitro, model predictions indicate that ester hydrolysis will eventually degrade the material with additional time, implying that this hydrogel may be appropriate for in vivo applications where temporally changing material properties enhance cell maturation prior to its replacement with host tissue.
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Metadata
- Authors
- Young, J. L., & Engler, A. J.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2011-02
- Volume
- 32
- Issue
- 4
- Pages
- 1002-1009
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2058657693
- Cited by
- 358
- References
- 38
- FWCI
- 9.92
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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