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Microfabrication of complex porous tissue engineering scaffolds using 3D projection stereolithography
Open accessAbstract
The success of tissue engineering will rely on the ability to generate complex, cell seeded three-dimensional (3D) structures. Therefore, methods that can be used to precisely engineer the architecture and topography of scaffolding materials will represent a critical aspect of functional tissue engineering. Previous approaches for 3D scaffold fabrication based on top-down and process driven methods are often not adequate to produce complex structures due to the lack of control on scaffold architecture, porosity, and cellular interactions. The proposed projection stereolithography (PSL) platform can be used to design intricate 3D tissue scaffolds that can be engineered to mimic the microarchitecture of tissues, based on computer aided design (CAD). The PSL system was developed, programmed and optimized to fabricate 3D scaffolds using gelatin methacrylate (GelMA). Variation of the structure and prepolymer concentration enabled tailoring the mechanical properties of the scaffolds. A dynamic cell seeding method was utilized to improve the coverage of the scaffold throughout its thickness. The results demonstrated that the interconnectivity of pores allowed for uniform human umbilical vein endothelial cells (HUVECs) distribution and proliferation in the scaffolds, leading to high cell density and confluency at the end of the culture period. Moreover, immunohistochemistry results showed that cells seeded on the scaffold maintained their endothelial phenotype, demonstrating the biological functionality of the microfabricated GelMA scaffolds.
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“Results from 3D studies demonstrate that increasing the dimensionality of extracellular matrix (ECM) around cells from 2D to 3D can significantly impact cell proliferation, differentiation, mechano-responses, and cell survival (2,10,41).”
From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [41]
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Cites
Matrix elasticity directs stem cell lineage specification
Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915
Langer, R., & Vacanti, J. P. (1993) · Science · doi:10.1126/science.8493529 · Cited by 9,893
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
Discher, D. E., Janmey, P., & Wang, Y.-L. (2005) · Science · doi:10.1126/science.1116995 · Cited by 6,382 · cited 1×
“Results obtained were found to be within a physiologic range (0e800 kPa) [65] and prepolymer concentration and scaffold geometry were shown to enable the tailoring of the mechanical properties of the hydrogels.”
BackgroundLutolf, M. P., & Hubbell, J. A. (2005) · Nature Biotechnology · doi:10.1038/nbt1055 · Cited by 4,551 · cited 1×
“One of the essential criteria to develop functional engineered tissues is to reproduce the 3D tissue architecture and provide a biologically relevant environment to the cells in order for them to maintain viability and to proliferate on the scaffolding material [20,54,55].”
BackgroundPorous scaffold design for tissue engineering
Hollister, S. J. (2005) · Nature Materials · doi:10.1038/nmat1421 · Cited by 3,979 · cited 1×
“applications are designed to closely mimic the physiologic environment, including the geometrical, topographical and physical features of the targeted tissue [2,3].”
BackgroundTaking Cell-Matrix Adhesions to the Third Dimension
Cukierman, E., Pankov, R., Stevens, D. R., & Yamada, K. M. (2001) · Science · doi:10.1126/science.1064829 · Cited by 3,027 · cited 1×
“The ability to fabricate three dimensional (3D) scaffolds having specifically designed microarchitecture reproducing the in vivo environment could elicit a physiologically relevant response and result into improved functionality of the tissue [4].”
BackgroundA review on stereolithography and its applications in biomedical engineering
Melchels, F. P. W., Feijén, J., & Grijpma, D. W. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.04.050 · Cited by 2,473
Cell-laden microengineered gelatin methacrylate hydrogels
Nichol, J. W., Koshy, S. T., Bae, H., Hwang, C. M., Yamanlar, S., & Khademhosseini, A. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.03.064 · Cited by 2,377 · cited 5×
“In the present study, a custom designed projection stereolithography (PSL) system was used to control the 3D microarchitecture of collagen-based gelatin methacrylate (GelMA) hydrogels [21,43].”
BackgroundMethodInfluentialSelf-citationElectrospinning of Collagen Nanofibers
Matthews, J. A., Wnek, G. E., Simpson, D. G., & Bowlin, G. L. (2002) · Biomacromolecules · doi:10.1021/bm015533u · Cited by 2,253 · cited 1×
“To produce microctructured scaffolds, multiple techniques such as solvent casting [9], particulate leaching [10,11], gas foaming [12], freeze drying [13] and more recently electrospinning [14] and laser sintering [15] have been developed.”
MethodMicroscale technologies for tissue engineering and biology
Khademhosseini, A., Langer, R., Borenstein, J. T., & Vacanti, J. P. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0507681102 · Cited by 1,553 · cited 2×
“Scaffold properties at the microscale are known to influence cell-ECM interactions and to dictate the mechanical properties and degradation behavior of a biomaterial [25].”
BackgroundSelf-citation
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2 of the 68 references couldn’t be matched to a known paper.
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Items 301–400 of 641
A microfabricated magnetic actuation device for mechanical conditioning of arrays of 3D microtissues
Xu, F., Zhao, R., Liu, A. S., Metz, T. E., Shi, Y., Bose, P., & Reich, D. H. (2015) · Lab on a Chip · doi:10.1039/c4lc01395f · Cited by 37
Regenerative therapies for tympanic membrane
Anand, S., Danti, S., Moroni, L., & Mota, C. (2022) · Progress in Materials Science · doi:10.1016/j.pmatsci.2022.100942 · Cited by 36
Orr, A., Kalantarnia, F., Nazir, S., Bolandi, B., Alderson, D., O’Grady, K., Hoorfar, M., Julian, L. M., & Willerth, S. M. (2025) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2025.115524 · Cited by 41
Bioengineering the gut: future prospects of regenerative medicine
Bitar, K. N., & Zakhem, E. (2016) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/nrgastro.2016.124 · Cited by 35
A review on the challenges of 3D printing of organic powders
Whyte, D. J., Rajkhowa, R., Allardyce, B. J., & Kouzani, A. Z. (2019) · Bioprinting · doi:10.1016/j.bprint.2019.e00057 · Cited by 38
Qi, A., Hoo, S. P., Friend, J. R., Yeo, L. Y., Yue, Z., & Chan, P. P. Y. (2013) · Advanced Healthcare Materials · doi:10.1002/adhm.201300155 · Cited by 35
A review on 3D printing functional brain model
Samanipour, R., Tahmooressi, H., Nejad, H. R., Hirano, M., Shin, S. R., & Hoorfar, M. (2022) · Biomicrofluidics · doi:10.1063/5.0074631 · Cited by 37
3D printing of polyurethane biomaterials
Hung, K., Tseng, C., & Hsu, S. (2016) · Elsevier eBooks · doi:10.1016/b978-0-08-100614-6.00005-6 · Cited by 37
Ahn, S. H., Lee, J., Park, S. A., & Kim, W. D. (2016) · Tissue Engineering and Regenerative Medicine · doi:10.1007/s13770-016-0148-1 · Cited by 38
“However, it is generally expensive, produces small moldings, and the available raw materials and colors are limited [53-56].”
BackgroundSelf-citationLow Cost Printer for DLP Stereolithography
Valentinčič, J., Peroša, M., Jerman, M., Sabotin, I., & Lebar, A. (2017) · Strojniški vestnik – Journal of Mechanical Engineering · doi:10.5545/sv-jme.2017.4591 · Cited by 34
“A DLP stereolithography is used in various fields including medical applications, where biocompatible and biodegra dable materials must be used [4] and [5], and oceanography [6] for a better understanding and restoration of fragile marine ecosystems.”
Method3D Printing of Scaffolds for Tissue Engineering
Liu, J.-Y., & Yan, C. (2018) · InTech eBooks · doi:10.5772/intechopen.78145 · Cited by 35
Advances in Photocrosslinkable Materials for 3D Bioprinting
Zhang, W., Ye, W., & Yan, Y. (2021) · Advanced Engineering Materials · doi:10.1002/adem.202100663 · Cited by 34
Wattanaanek, N., Suttapreyasri, S., & Samruajbenjakun, B. (2022) · Journal of Functional Biomaterials · doi:10.3390/jfb13020047 · Cited by 36
“2022, 13, 47 2 of 16 For example, SLA needs a monomer to complete the polymerization after printing [4] and requires removal of powders [5].”
MethodLiquid-Based 4D Printing of Shape Memory Nanocomposites: A Review
Alsaadi, M., Hinchy, E. P., McCarthy, C. T., Moritz, V. F., Zhuo, S., Fuenmayor, E. A., & Devine, D. M. (2023) · Journal of Manufacturing and Materials Processing · doi:10.3390/jmmp7010035 · Cited by 36
Liu, S., Li, Z., Wang, Q., Han, J., Wang, W., Li, S., Liu, H., Guo, S., Zhang, J., Ge, K., et al. (2021) · ACS Applied Bio Materials · doi:10.1021/acsabm.1c00967 · Cited by 34
Biomaterials for Sustained and Controlled Delivery of Small Drug Molecules
Mehtani, D., Seth, A., Sharma, P. A., Maheshwari, N., Kapoor, D. U., Shrivastava, S. K., & Tekade, R. K. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-814427-5.00004-4 · Cited by 36
Matrix biophysical cues direct mesenchymal stromal cell functions in immunity
Wong, S. W., Lenzini, S. B., Giovanni, R., Clark-Knowles, K. V., & Shin, J. (2021) · Acta Biomaterialia · doi:10.1016/j.actbio.2021.07.075 · Cited by 34
Sharaf, A., Roos, B., Timmerman, R., Kremers, G., Bajramovic, J. J., & Accardo, A. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.926642 · Cited by 32
“, 2021), stereolithography (Gauvin et al., 2012), and two-photon polymerization (2PP) (Lemma et al.”
Comeau, P., & Willett, T. L. (2020) · Materials Science and Engineering C · doi:10.1016/j.msec.2020.110937 · Cited by 31
Therapeutic application of hydrogels for bone-related diseases
Liu, X., Sun, S., Wang, N., Kang, R., Xie, L., & Liu, X. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.998988 · Cited by 33
Forbidden Chemistry: Two-Photon Pathway in [2+2] Cycloaddition of Maleimides
Tsurkan, M. V., Jungnickel, C., Schlierf, M., & Werner, C. (2017) · Journal of the American Chemical Society · doi:10.1021/jacs.7b04484 · Cited by 30
Zhou, Y., Hu, Z., Du, D., & Tan, G. Z. (2018) · The International Journal of Advanced Manufacturing Technology · doi:10.1007/s00170-018-2899-4 · Cited by 30
3D bioprinting of vascular conduits for pediatric congenital heart repairs
Lee, W., Hong, Y., & Dai, G. (2019) · Translational research · doi:10.1016/j.trsl.2019.03.007 · Cited by 31
Norris, S. C. P., Delgado, S. M., & Kasko, A. M. (2019) · Polymer Chemistry · doi:10.1039/c9py00308h · Cited by 32
Szklanny, A. A., Debbi, L., Merdler, U., Neale, D. B., Muñiz, A. J., Kaplan, B., Guo, S., Lahann, J., & Levenberg, S. (2019) · Advanced Functional Materials · doi:10.1002/adfm.201901335 · Cited by 30
Photopolymerizable pullulan: Synthesis, self-assembly and inkjet printing
Mugnaini, G., Resta, C., Poggi, G., & Bonini, M. (2021) · Journal of Colloid and Interface Science · doi:10.1016/j.jcis.2021.02.074 · Cited by 30
Rat Calvarial Bone Regeneration by 3D-Printed β-Tricalcium Phosphate Incorporating MicroRNA-200c
Remy, M. T., Akkouch, A., Li, H., Eliason, S. L., Sweat, M., Krongbaramee, T., Fei, F., Qian, F., Amendt, B. A., Song, X., et al. (2021) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.0c01756 · Cited by 30
Three-Dimensional Photochemical Printing of Thermally Activated Polymer Foams
Seo, S. E., Kwon, Y., Dolinski, N. D., Sample, C. S., Self, J. L., Bates, C. M., Valentine, M. T., & Hawker, C. J. (2021) · ACS Applied Polymer Materials · doi:10.1021/acsapm.1c00726 · Cited by 30
Non‐Covalent Photo‐Patterning of Gelatin Matrices Using Caged Collagen Mimetic Peptides
Li, Y., San, B. H., Kessler, J. L., Kim, J. H., Xu, Q., Hanes, J. S., & Yu, S. M. (2014) · Macromolecular Bioscience · doi:10.1002/mabi.201400436 · Cited by 29
Micropatterning Electrospun Scaffolds to Create Intrinsic Vascular Networks
Jeffries, E. M., Nakamura, S., Lee, K., Clampffer, J., Ijima, H., & Wang, Y. (2014) · Macromolecular Bioscience · doi:10.1002/mabi.201400306 · Cited by 30
New Methods in Tissue Engineering: Improved Models for Viral Infection
Ramanan, V., Scull, M. A., Sheahan, T. P., Rice, C. M., & Bhatia, S. N. (2014) · Annual Review of Virology · doi:10.1146/annurev-virology-031413-085437 · Cited by 28
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Sharma, A. K., & Gupta, S. (2020) · JOM · doi:10.1007/s11837-020-04003-z · Cited by 28
Physicochemical parameters that underlie inkjet printing for medical applications
Machekposhti, S. A., Movahed, S., & Narayan, R. J. (2020) · Biophysics Reviews · doi:10.1063/5.0011924 · Cited by 31
Three-dimensional printing of biomaterials and soft materials
Bandyopadhyay, A. K., Vahabzadeh, S., Shivaram, A., & Bose, S. (2015) · MRS Bulletin · doi:10.1557/mrs.2015.274 · Cited by 28
Photoinitiator-free 3D scaffolds fabricated by excimer laser photocuring
Farkas, B., Dante, S., & Brandi, F. (2016) · Nanotechnology · doi:10.1088/1361-6528/28/3/034001 · Cited by 28
Yoo, D.-J. (2014) · International Journal of Precision Engineering and Manufacturing · doi:10.1007/s12541-014-0571-y · Cited by 27
Larsen, E., Larsen, N. B., Almdal, K., Larsen, E., Larsen, N. B., & Almdal, K. (2016) · Journal of Polymer Science Part B Polymer Physics · doi:10.1002/polb.24007 · Cited by 27
Liu, C., Tong, J., Ma, J., Wang, D., Xu, F., Liu, Y., Chen, Z., & Lao, C. (2019) · Journal of Nanomaterials · doi:10.1155/2019/1291067 · Cited by 28
Weinandy, S., Laffar, S., Unger, R. E., Flanagan, T. C., Lösel, R., Kirkpatrick, C. J., van Zandvoort, M. A. M. J., Hermanns‐Sachweh, B., Dreier, A., Klee, D., et al. (2014) · Tissue Engineering Part A · doi:10.1089/ten.tea.2013.0330 · Cited by 26
Colasante, C., Sanford, Z., Garfein, E. S., & Tepper, O. M. (2016) · Current Surgery Reports · doi:10.1007/s40137-016-0127-4 · Cited by 28
Matbouei, A., Fathi, A., Rabiee, S. M., & Shirzad, M. (2018) · Materials Technology · doi:10.1080/10667857.2018.1541212 · Cited by 26
“Also, scaffolds play the role of extracellular matrices which are able to drive and support three-dimensional tissue regeneration [1,2].”
BackgroundBiodegradable microrobots for targeting cell delivery
Tirgar, P., & Bagheri‐Khoulenjani, S. (2017) · Medical Hypotheses · doi:10.1016/j.mehy.2017.02.015 · Cited by 26
“In a research by Khademhosseini et al.[62], gelatin methacrylol was used in combination with a projection stereolithography system to fabricate 3D structures of woodpile and hexagonal structures.”
MethodEngineering of Removing Sacrificial Materials in 3D-Printed Microfluidics
Yin, P., Hu, B., Yi, L., Xiao, C., Cao, X., Zhao, L. Q., & Shi, H. (2018) · Micromachines · doi:10.3390/mi9070327 · Cited by 26
Agarwal, T., Rustagi, A., Das, J., & Maiti, T. K. (2018) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2018.08.062 · Cited by 26
3D-printed microrobots for biomedical applications
Wei, K., Tang, C., Ma, H., Fang, X., & Yang, R. (2024) · Biomaterials Science · doi:10.1039/d4bm00674g · Cited by 27
3D scaffold fabrication by mask projection excimer laser stereolithography
Beke, S., Farkas, B., Romano, I., & Brandi, F. (2014) · Optical Materials Express · doi:10.1364/ome.4.002032 · Cited by 25
Fathi, A., Lee, S., Breen, A., Shirazi, A. N., Valtchev, P., & Dehghani, F. (2014) · European Polymer Journal · doi:10.1016/j.eurpolymj.2014.07.011 · Cited by 25
Wang, B., Liu, J., Chen, K., Wang, Y., & Shao, Z. (2020) · Polymer Engineering and Science · doi:10.1002/pen.25336 · Cited by 25
Nguyen, H., Bazaka, O., Bazaka, K., Crawford, R. J., & Ivanova, E. P. (2020) · Trends in biotechnology · doi:10.1016/j.tibtech.2019.12.004 · Cited by 25
In vitro Characterization of Insulin−Producing β-Cell Spheroids
Ntamo, Y., Samodien, E., Burger, J., Muller, N., Muller, C. J. F., & Chellan, N. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.623889 · Cited by 27
“…dynamic cell and signaling environments, thus increasing the preclinical value of 3D models in the field of drug discovery and as predictors of potential therapeutic outcomes (Chang and Hughes-Fulford, 2009; Fey and Wrzesinski, 2012; Gauvin et al., 2012; Fennema et al., 2013; Jacobi et al., 2017).”
BackgroundBaur, E., Hirsch, M., & Amstad, E. (2023) · Advanced Materials Technologies · doi:10.1002/admt.202201763 · Cited by 24
Protein-Based Materials in Load-Bearing Tissue-Engineering Applications
Sayın, E., Baran, E. T., & Hasırcı, V. (2014) · Regenerative Medicine · doi:10.2217/rme.14.52 · Cited by 25
Printing@Clinic: From Medical Models to Organ Implants
Zhao, H., Yang, F., Fu, J., Gao, Q., Liu, A., Sun, M., & He, Y. (2017) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.7b00542 · Cited by 25
Wang, B., Ding, G., Chen, K., Jia, S., Wei, J., Wang, Y., He, R., & Shao, Z. (2020) · Journal of Applied Polymer Science · doi:10.1002/app.49164 · Cited by 25
Digital micro-mirror device -based light curing technology and its biological applications
Wang, Z., Yang, W., Qin, Y., Liang, W., Yu, H., & Liu, L. (2021) · Optics & Laser Technology · doi:10.1016/j.optlastec.2021.107344 · Cited by 28
Ning, L., Chen, J., Sun, J., Liu, Y., Yi, D., & Cao, J. (2021) · ACS Omega · doi:10.1021/acsomega.1c01232 · Cited by 24
Control over the formation of supramolecular material objects using reaction–diffusion
Lovrak, M., Hendriksen, W. E., Kreutzer, M. T., van Steijn, V., Eelkema, R., & van Esch, J. H. (2019) · Soft Matter · doi:10.1039/c8sm02588f · Cited by 24
Zhao, R., Zhou, Y., Jie, K., Yang, J., Perrier, S., & Huang, F. (2019) · Chinese Journal of Polymer Science · doi:10.1007/s10118-019-2305-1 · Cited by 24
Photopolymerizable hydrogels in regenerative medicine and drug delivery
Pereira, R. F., & Bártolo, P. (2014) · doi:10.4155/ebo.13.650 · Cited by 24
Pazhamannil, R. V., & AlKhedher, M. A. (2024) · Biomedical Materials · doi:10.1088/1748-605x/ad9dce · Cited by 23
Light-Controlled Microbots in Biomedical Application: A Review
Jamil, M. F., Pokharel, M., & Park, K. (2022) · Applied Sciences · doi:10.3390/app122111013 · Cited by 24
Emerging breakthroughs in biomaterials for orthopedic applications: A comprehensive review
Mahmud, M. Z. A., Mobarak, M. H., Hossain, N., Islam, M. A., & Rayhan, M. T. (2023) · Bioprinting · doi:10.1016/j.bprint.2023.e00323 · Cited by 23
Revolutionizing healthcare: Emerging frontiers in 3D bioprinting of tissues and organs
Ochieng, B. O., Arroyan, Y. N., Onyango, B., Kong, L., Wang, G., & Ye, Z. (2024) · European Polymer Journal · doi:10.1016/j.eurpolymj.2024.113210 · Cited by 22
Zhao, S., Zhao, H., Zhang, X., Li, Y., & Du, Y. (2013) · Lab on a Chip · doi:10.1039/c3lc50183c · Cited by 23
Three dimensional live cell lithography
Linnenberger, A. M., Bodine, M. I., Fiedler, C., Roberts, J. J., Skaalure, S. C., Quinn, J. P., Bryant, S. J., Cole, M. C., & McLeod, R. R. (2013) · Optics Express · doi:10.1364/oe.21.010269 · Cited by 23
Cheng, K. W., Alhasan, L., Rezk, A. R., Al‐Abboodi, A., Doran, P. M., Yeo, L. Y., & Chan, P. P. Y. (2019) · Biofabrication · doi:10.1088/1758-5090/ab4cca · Cited by 24
Biofabrication strategies for engineering heterogeneous artificial tissues
Ambhorkar, P., Rakin, R. H., Wang, Z., Kumar, H., & Kim, K. (2020) · Additive manufacturing · doi:10.1016/j.addma.2020.101459 · Cited by 25
Properties of PLDLA/bioglass scaffolds produced by selective laser sintering
Salmoria, G. V., Pereira, R. V., Fredel, M. C., & Casadei, A. P. M. (2017) · Polymer Bulletin · doi:10.1007/s00289-017-2093-0 · Cited by 22
“Therefore, methods that can be used for the precise engineering of the architecture and topography of scaffolding materials will represent a critical aspect of functional tissue engineering [5].”
MethodBioengineered Efficacy Models of Skin Disease: Advances in the Last 10 Years
Stanton, D. N., Ganguli-Indra, G., Indra, A. K., & Karande, P. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14020319 · Cited by 22
Tomal, W., & Ortyl, J. (2022) · European Polymer Journal · doi:10.1016/j.eurpolymj.2022.111588 · Cited by 22
Călin, B. Ş., & Păun, I. A. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232214270 · Cited by 22
“5D structures, whereas, for 3D structures, they are able to produce only very simple architectures, involving multistep fabrication processes [14,15].”
BackgroundKeeping It Organized: Multicompartment Constructs to Mimic Tissue Heterogeneity
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Baah-Dwomoh, A., Rolong, A., Gatenholm, P., & Davalos, R. V. (2015) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-015-6445-0 · Cited by 22
“Multiple techniques such as sintering, salt leeching, gas foaming, and electrospinning have been used to create micro structured architectures within scaffolds (Ellis and Yannas 1996; Gauvin et al. 2012; Harris et al. 1998; Matthews et al. 2002).”
Using glucosamine to improve the properties of photocrosslinked gelatin scaffolds
Suo, H., Xu, K., & Zheng, X. (2014) · Journal of Biomaterials Applications · doi:10.1177/0885328214551009 · Cited by 23
“In recent years, GelMA hydrogel has attracted great attention in tissue engineering and stem cell bioengineering, mainly due to the large amount of cell binding motifs on gelatin molecule and the tunable mechanical robustness of hydrogel.(40,41) In this study, AGA and GelMA were successfully used to generate composite…”
BackgroundMethodConstruction and control of 3D porous structure based on vapor deposition on sublimation solids
Tung, H., Sun, T.-P., Sun, H., Guan, Z., Hu, S.-K., Chao, L., & Chen, H. (2017) · Applied Materials Today · doi:10.1016/j.apmt.2016.12.005 · Cited by 22
The construction of in vitro tumor models based on 3D bioprinting
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Stereolithography of (meth)acrylate‐based photocurable resin: Thermal and mechanical properties
Zirak, N., Shirinbayan, M., Benfriha, K., Deligant, M., & Tcharkhtchi, A. (2022) · Journal of Applied Polymer Science · doi:10.1002/app.52248 · Cited by 21
3D printed elastomers with Sylgard-184-like mechanical properties and tuneable degradability
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Soto, F., Wang, J., Deshmukh, S. S., & Demirci, U. (2020) · Advanced Intelligent Systems · doi:10.1002/aisy.202000193 · Cited by 21
Advanced tumor organoid bioprinting strategy for oncology research
Cui, X., Jiao, J., Yang, L., Wang, Y., Jiang, W., Yu, T., Li, M., Zhang, H., Chao, B., Wang, Z., et al. (2024) · Materials Today Bio · doi:10.1016/j.mtbio.2024.101198 · Cited by 25
“This method does not subject cells to mechanical stress, which is beneficial for maintaining cell viability, and provides the highest resolution among the three printing methods [133] (Fig.”
MethodDynamic high-resolution optical trapping of ultracold atoms
Gauthier, G., Bell, T. A., Stilgoe, A. B., Baker, M. A., Rubinsztein-Dunlop, H. H., & Neely, T. W. (2021) · Advances in atomic, molecular, and optical physics · doi:10.1016/bs.aamop.2021.04.001 · Cited by 25
Mandal, K., Sangabathuni, S., Haghniaz, R., Kawakita, S., Mecwan, M. M., Nakayama, A., Zhang, X., Edalati, M., Huang, W., Hernández, A. L., et al. (2023) · Acta Biomaterialia · doi:10.1016/j.actbio.2023.01.030 · Cited by 20
Self-citation3D-Printable Biodegradable Polyester Tissue Scaffolds for Cell Adhesion
Sirrine, J. M., Pekkanen, A. M., Nelson, A. M., Chartrain, N. A., Williams, C. B., & Long, T. E. (2015) · Australian Journal of Chemistry · doi:10.1071/ch15327 · Cited by 20
Rodriguez, D. E., Güiza-Argüello, V. R., Ochoa, O. O., Gharat, T. P., Sue, H., Lafdi, K., & Hahn, M. S. (2015) · Carbon · doi:10.1016/j.carbon.2015.10.086 · Cited by 20
Recent Advances in Additive Biomanufacturing
Pereira, R. F., & Bártolo, P. (2014) · Elsevier eBooks · doi:10.1016/b978-0-08-096532-1.01009-8 · Cited by 20
Rodrigo-Vázquez, C. S., Kamboj, N., Aghayan, M., Sáez, A., Aza, A. H. D., Rodriguez, M. A., & Hussainova, I. (2020) · Ceramics International · doi:10.1016/j.ceramint.2020.07.171 · Cited by 20
Fabrication Speed Optimization for High-resolution 3D-printing of Bioresorbable Vascular Scaffolds
Ware, H. O. T., Farsheed, A. C., Baker, E., Ameer, G. A., & Sun, C. (2017) · Procedia CIRP · doi:10.1016/j.procir.2017.04.038 · Cited by 20
Effects of Surface Energy Reducing Agents on Adhesion Force in Liquid Bridge Microstereolithography
Alamdari, A. B., Lee, J., Kim, M., Emon, O. F., Dhinojwala, A., & Choi, J. (2020) · Additive manufacturing · doi:10.1016/j.addma.2020.101522 · Cited by 17
Self-citationSood, A., Kumar, A., Gupta, V. K., Kim, C. M., & Han, S. S. (2022) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.2c01080 · Cited by 21
Current Status of Tissue Engineering Heart Valve
Shinoka, T., & Miyachi, H. (2016) · World Journal for Pediatric and Congenital Heart Surgery · doi:10.1177/2150135116664873 · Cited by 19
Li, P., Yu, H., Liu, N., Wang, F., Lee, G., Wang, Y., Liu, L., & Li, W. J. (2018) · Biomaterials Science · doi:10.1039/c7bm01153a · Cited by 19
Polypeptide Thermogels as Three-Dimensional Scaffolds for Cells
Patel, M., Park, S., Lee, H. J., & Jeong, B. (2018) · Tissue Engineering and Regenerative Medicine · doi:10.1007/s13770-018-0148-4 · Cited by 19
Mechanical properties of porous photo-crosslinked poly(trimethylene carbonate) network films
van Bochove, B., & Grijpma, D. W. (2020) · European Polymer Journal · doi:10.1016/j.eurpolymj.2020.110223 · Cited by 18
Polymeric Bioinks for 3D Hepatic Printing
Sarkar, J., Kamble, S. C., & Kashikar, N. C. (2021) · Chemistry · doi:10.3390/chemistry3010014 · Cited by 19
“An optimum gelatin concentration and crosslinking method will provide a bioprinted hepatic construct with desired mechanical strength and biocompatibility [51,52].”
MethodZhang, D., Davoodi, P., Li, X., Liu, Y., Wang, W., & Huang, Y. Y. S. (2020) · Scientific Reports · doi:10.1038/s41598-020-74542-7 · Cited by 18
Hybrid biomanufacturing systems applied in tissue regeneration
Liu, F., Quan, R., Vyas, C., & Aslan, E. (2022) · International Journal of Bioprinting · doi:10.18063/ijb.v9i1.646 · Cited by 20
“VP-based bioprinting process has been widely applied for tissue engineering due to its superior resolution and accuracy, which is up to 20 m in SLA and DLP and up to sub-100 nm region in 2PP.”
BackgroundInfluentialLee, H.-Y., & Lee, J. W. (2023) · Journal of Functional Biomaterials · doi:10.3390/jfb14040196 · Cited by 18
“In the ase of DLP, planar light from a light source is selectively reflected, forming an im ged light layer [35,51,52].”
BackgroundSelf-citationECM-based macroporous sponges release essential factors to support the growth of hematopoietic cells
Soffer-Tsur, N., Peer, D., & Dvir, T. (2016) · Journal of Controlled Release · doi:10.1016/j.jconrel.2016.09.021 · Cited by 18
“On the other hand, the pores should be small enough to enable sufficient contact between the cells and the surface, facilitating extracellular signaling and delivery of nutrients [30,31].”
Bioinspired reorientation strategies for application in micro/nanorobotic control
Ghanbari, A. (2020) · Journal of Micro-Bio Robotics · doi:10.1007/s12213-020-00130-7 · Cited by 20
Microfabrication Technology in Tissue Engineering
Cha, C., Piraino, F., & Khademhosseini, A. (2014) · Elsevier eBooks · doi:10.1016/b978-0-12-420145-3.00009-2 · Cited by 18
Self-citation
Metadata
- Authors
- Gauvin, R., Chen, Y.-C., Lee, J. W., Soman, P., Zorlutuna, P., Nichol, J. W., Bae, H., Chen, S., & Khademhosseini, A.
- Affiliations
- Brigham and Women's Hospital (US)
- University of California San Diego (US)
- Corresponding author
- Chen, Ying-Chieh
- Chen, Shaochen
- Khademhosseini, Ali
- ORCID
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2012-05
- Volume
- 33
- Issue
- 15
- Pages
- 3824-3834
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC3766354
- OpenAlex
- W2164279637
- Cited by
- 641
- References
- 68
- Citations by year
- 2026: 13 · 2025: 30 · 2024: 28 · 2023: 42 · 2022: 57 · 2021: 58 · 2020: 66 · 2019: 69 · 2018: 59 · 2017: 53 · 2016: 43 · 2015: 43 · 2014: 41 · 2013: 28 · 2012: 10
- FWCI
- 22.25
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (HL099073, EB012597, HL092836)
- Fonds Québécois de la Recherche sur la Nature et les Technologies
- National Science Foundation (DMR0847287)
- Office of Naval Research
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Keywords
- projection stereolithography, tissue engineering scaffolds, gelatin methacryloyl, 3D microfabrication, porous scaffolds, scaffold porosity, human umbilical vein endothelial cells, endothelial phenotype, scaffold mechanical properties, scaffold architecture
- MeSH terms
- Fluorescent Antibody Technique, Humans, Porosity, Microscopy, Confocal, Tissue Engineering, Green Fluorescent Proteins, Tissue Scaffolds, Microtechnology, Human Umbilical Vein Endothelial Cells
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