The DOI matches its Crossref record; 66 of the 68 references it cites were found in a registry or an index.
Microfabrication of complex porous tissue engineering scaffolds using 3D projection stereolithography
Open accessAbstract
The success of tissue engineering will rely on the ability to generate complex, cell seeded three-dimensional (3D) structures. Therefore, methods that can be used to precisely engineer the architecture and topography of scaffolding materials will represent a critical aspect of functional tissue engineering. Previous approaches for 3D scaffold fabrication based on top-down and process driven methods are often not adequate to produce complex structures due to the lack of control on scaffold architecture, porosity, and cellular interactions. The proposed projection stereolithography (PSL) platform can be used to design intricate 3D tissue scaffolds that can be engineered to mimic the microarchitecture of tissues, based on computer aided design (CAD). The PSL system was developed, programmed and optimized to fabricate 3D scaffolds using gelatin methacrylate (GelMA). Variation of the structure and prepolymer concentration enabled tailoring the mechanical properties of the scaffolds. A dynamic cell seeding method was utilized to improve the coverage of the scaffold throughout its thickness. The results demonstrated that the interconnectivity of pores allowed for uniform human umbilical vein endothelial cells (HUVECs) distribution and proliferation in the scaffolds, leading to high cell density and confluency at the end of the culture period. Moreover, immunohistochemistry results showed that cells seeded on the scaffold maintained their endothelial phenotype, demonstrating the biological functionality of the microfabricated GelMA scaffolds.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Results from 3D studies demonstrate that increasing the dimensionality of extracellular matrix (ECM) around cells from 2D to 3D can significantly impact cell proliferation, differentiation, mechano-responses, and cell survival (2,10,41).”
From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [41]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Matrix elasticity directs stem cell lineage specification
Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915
Langer, R., & Vacanti, J. P. (1993) · Science · doi:10.1126/science.8493529 · Cited by 9,893
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
Discher, D. E., Janmey, P., & Wang, Y.-L. (2005) · Science · doi:10.1126/science.1116995 · Cited by 6,382 · cited 1×
“Results obtained were found to be within a physiologic range (0e800 kPa) [65] and prepolymer concentration and scaffold geometry were shown to enable the tailoring of the mechanical properties of the hydrogels.”
BackgroundLutolf, M. P., & Hubbell, J. A. (2005) · Nature Biotechnology · doi:10.1038/nbt1055 · Cited by 4,551 · cited 1×
“One of the essential criteria to develop functional engineered tissues is to reproduce the 3D tissue architecture and provide a biologically relevant environment to the cells in order for them to maintain viability and to proliferate on the scaffolding material [20,54,55].”
BackgroundPorous scaffold design for tissue engineering
Hollister, S. J. (2005) · Nature Materials · doi:10.1038/nmat1421 · Cited by 3,979 · cited 1×
“applications are designed to closely mimic the physiologic environment, including the geometrical, topographical and physical features of the targeted tissue [2,3].”
BackgroundTaking Cell-Matrix Adhesions to the Third Dimension
Cukierman, E., Pankov, R., Stevens, D. R., & Yamada, K. M. (2001) · Science · doi:10.1126/science.1064829 · Cited by 3,027 · cited 1×
“The ability to fabricate three dimensional (3D) scaffolds having specifically designed microarchitecture reproducing the in vivo environment could elicit a physiologically relevant response and result into improved functionality of the tissue [4].”
BackgroundA review on stereolithography and its applications in biomedical engineering
Melchels, F. P. W., Feijén, J., & Grijpma, D. W. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.04.050 · Cited by 2,473
Cell-laden microengineered gelatin methacrylate hydrogels
Nichol, J. W., Koshy, S. T., Bae, H., Hwang, C. M., Yamanlar, S., & Khademhosseini, A. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.03.064 · Cited by 2,377 · cited 5×
“In the present study, a custom designed projection stereolithography (PSL) system was used to control the 3D microarchitecture of collagen-based gelatin methacrylate (GelMA) hydrogels [21,43].”
BackgroundMethodInfluentialSelf-citationElectrospinning of Collagen Nanofibers
Matthews, J. A., Wnek, G. E., Simpson, D. G., & Bowlin, G. L. (2002) · Biomacromolecules · doi:10.1021/bm015533u · Cited by 2,253 · cited 1×
“To produce microctructured scaffolds, multiple techniques such as solvent casting [9], particulate leaching [10,11], gas foaming [12], freeze drying [13] and more recently electrospinning [14] and laser sintering [15] have been developed.”
MethodMicroscale technologies for tissue engineering and biology
Khademhosseini, A., Langer, R., Borenstein, J. T., & Vacanti, J. P. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0507681102 · Cited by 1,553 · cited 2×
“Scaffold properties at the microscale are known to influence cell-ECM interactions and to dictate the mechanical properties and degradation behavior of a biomaterial [25].”
BackgroundSelf-citationStructural and Rheological Properties of Methacrylamide Modified Gelatin Hydrogels
Van Den Bulcke, A. I., Bogdanov, B., De Rooze, N., Schacht, E. H., Cornelissen, M., & Berghmans, H. (2000) · Biomacromolecules · doi:10.1021/bm990017d · Cited by 1,536 · cited 1×
“In the present study, a custom designed projection stereolithography (PSL) system was used to control the 3D microarchitecture of collagen-based gelatin methacrylate (GelMA) hydrogels [21,43].”
MethodHuman tissue-engineered blood vessels for adult arterial revascularization
L’Heureux, N., Dusserre, N., König, G., Victor, B. E., Keire, P. A., Wight, T. N., Chronos, N. A. F., Kyles, A. E., Gregory, C. R., Hoyt, G. E., et al. (2006) · Nature Medicine · doi:10.1038/nm1364 · Cited by 1,083
Burdick, J. A., & Anseth, K. S. (2002) · Biomaterials · doi:10.1016/s0142-9612(02)00176-x · Cited by 1,008
Accordion-like honeycombs for tissue engineering of cardiac anisotropy
Engelmayr, G. C., Cheng, M., Bettinger, C. J., Borenstein, J. T., Langer, R., & Freed, L. E. (2008) · Nature Materials · doi:10.1038/nmat2316 · Cited by 848 · cited 1×
“Precisely defined accordion-like microstructures having biomimetic mechanical properties were shown to mimic the function of adult rat right ventricular myocardium [18] and microengineered 3D porous structure of a multi-layered elastomeric scaffolds allowed heart cells to be readily seeded by perfusion throughout the…”
BackgroundMicroengineered hydrogels for tissue engineering
Khademhosseini, A., & Langer, R. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.07.021 · Cited by 830
Self-citationEffect of Pore Size and Void Fraction on Cellular Adhesion, Proliferation, and Matrix Deposition
Zeltinger, J., Sherwood, J. K., Graham, D. A., Müller, R., & Griffith, L. G. (2001) · Tissue Engineering · doi:10.1089/107632701753213183 · Cited by 807 · cited 1×
“Interconnections between pores also play an important role in cell seeding, migration and nutrient transport during culture of the scaffolds [39,56].”
BackgroundA photolabile hydrogel for guided three-dimensional cell growth and migration
Luo, Y., & Shoichet, M. S. (2004) · Nature Materials · doi:10.1038/nmat1092 · Cited by 801
De novo reconstitution of a functional mammalian urinary bladder by tissue engineering
Oberpenning, F., Meng, J., Yoo, J., & Atala, A. (1999) · Nature Biotechnology · doi:10.1038/6146 · Cited by 800 · cited 1×
“The field of tissue engineering has so far provided technologies that are adequate for the replacement of connective tissues such as skin [5], cartilage [6], bladder [7] and blood vessels [8].”
BackgroundThree‐dimensional printing of porous ceramic scaffolds for bone tissue engineering
Seitz, H., Rieder, W., Irsen, S. H., Leukers, B., & Tille, C. (2005) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.30291 · Cited by 742 · cited 1×
“Manufacturing and fabrication technologies such as rapid prototyping [32,33], laser microstereolithography [34e36] and solid freeform fabrication (SFF) have evolved as methods to fabricate 3D scaffolds with micrometer scale resolution by using Computer-Aided-Design (CAD) programs [37].”
MethodMoutos, F. T., Freed, L. E., & Guilak, F. (2007) · Nature Materials · doi:10.1038/nmat1822 · Cited by 741 · cited 1×
“The design of precise and specific structures in the material also has the capability to tailor the mechanical properties of a 3D hydrogel material [63,64].”
BackgroundPolymeric Biomaterials with Degradation Sites for Proteases Involved in Cell Migration
West, J. L., & Hubbell, J. A. (1998) · Macromolecules · doi:10.1021/ma981296k · Cited by 635 · cited 1×
“It also provides built-in degradable moieties, which usually need to be added to synthetic polymers [28].”
BackgroundBiodegradable Polymer Scaffolds with Well-Defined Interconnected Spherical Pore Network
Ma, P. X., & Choi, J. (2001) · Tissue Engineering · doi:10.1089/107632701300003269 · Cited by 621
Mikos, A. G., Herring, S. W., Ochareon, P., Elisseeff, J. H., Lu, H. H., Kandel, R. A., Schöen, F. J., Toner, M., Mooney, D. J., Atala, A., et al. (2006) · Tissue Engineering · doi:10.1089/ten.2006.12.3307 · Cited by 598 · cited 1×
“To produce microctructured scaffolds, multiple techniques such as solvent casting [9], particulate leaching [10,11], gas foaming [12], freeze drying [13] and more recently electrospinning [14] and laser sintering [15] have been developed.”
MethodDirected 3D cell alignment and elongation in microengineered hydrogels
Aubin, H., Nichol, J. W., Hutson, C. B., Bae, H., Sieminski, A. L., Cropek, D. M., Akhyari, P., & Khademhosseini, A. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.05.056 · Cited by 540 · cited 2×
“It can also be used to encapsulate cells, is compatible with microfabrication processes and allow tissue growth for extended periods of time [21,49].”
BackgroundMethodSelf-citationMathematically defined tissue engineering scaffold architectures prepared by stereolithography
Melchels, F. P., Velders, A. H., Feijen, J., Grijpma, D. W., Bertoldi, K., & Gabbrielli, R. (2010) · Institutional Research Information System (Università degli Studi di Trento) · doi:10.1016/j.biomaterials.2010.05.068 · Cited by 533 · cited 1×
“Other investigators have previously designed highly porous and biophilic structures relying on Triply Periodic Minimal Surfaces (TPMS) models [36,44,45].”
BackgroundEngineered skin substitutes: practices and potentials
Supp, D. M., & Boyce, S. T. (2005) · Clinics in Dermatology · doi:10.1016/j.clindermatol.2004.07.023 · Cited by 519 · cited 1×
“The field of tissue engineering has so far provided technologies that are adequate for the replacement of connective tissues such as skin [5], cartilage [6], bladder [7] and blood vessels [8].”
BackgroundChan, V., Zorlutuna, P., Jeong, J. H., Kong, H., & Bashir, R. (2010) · Lab on a Chip · doi:10.1039/c004285d · Cited by 471
Self-citationCovalently immobilized gradients of bFGF on hydrogel scaffolds for directed cell migration
DeLong, S. A., Moon, J. J., & West, J. L. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.09.021 · Cited by 469
Fabrication of 3D hepatic tissues by additive photopatterning of cellular hydrogels
Tsang, V. L., Chen, A. A., Cho, L. M., Jadin, K. D., Sah, R. L., DeLong, S., West, J. L., & Bhatia, S. N. (2007) · The FASEB Journal · doi:10.1096/fj.06-7117com · Cited by 462
Tissue engineering of cartilage in space
Freed, L. E., Langer, R., Martín, I., Pellis, N. R., & Vunjak‐Novakovic, G. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.25.13885 · Cited by 434 · cited 1×
“The field of tissue engineering has so far provided technologies that are adequate for the replacement of connective tissues such as skin [5], cartilage [6], bladder [7] and blood vessels [8].”
BackgroundThree-Dimensional Photopatterning of Hydrogels Containing Living Cells
Liu, V. A., & Bhatia, S. N. (2002) · Biomedical Microdevices · doi:10.1023/a:1020932105236 · Cited by 399 · cited 2×
“Traditional design limitations, often related to mold shape and processing, were overcome by the use of these microfabrication methods, allowing for the generation of intricate structures [22,30].”
BackgroundRAJAGOPALAN, S., & Robb, R. A. (2006) · Medical Image Analysis · doi:10.1016/j.media.2006.06.001 · Cited by 392 · cited 2×
“Other investigators have previously designed highly porous and biophilic structures relying on Triply Periodic Minimal Surfaces (TPMS) models [36,44,45].”
BackgroundDhariwala, B., Hunt, E., & Boland, T. (2004) · Tissue Engineering · doi:10.1089/ten.2004.10.1316 · Cited by 390 · cited 1×
“Manufacturing and fabrication technologies such as rapid prototyping [32,33], laser microstereolithography [34e36] and solid freeform fabrication (SFF) have evolved as methods to fabricate 3D scaffolds with micrometer scale resolution by using Computer-Aided-Design (CAD) programs [37].”
MethodLu, Y., Mapili, G., Suhali, G., Chen, S., & Roy, K. (2006) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.30601 · Cited by 381 · cited 3×
“This PSL apparatus was previously fabricated and used to create 3D PEGbased biological scaffolds with complex internal architectures and spatial patterns in a high throughput fashion [31,40].”
BackgroundMethodSelf-citationFabrication of Gradient Hydrogels Using a Microfluidics/Photopolymerization Process
Burdick, J. A., Khademhosseini, A., & Langer, R. (2004) · Langmuir · doi:10.1021/la049298n · Cited by 374
Self-citationTissue-Engineered Blood Vessel for Adult Arterial Revascularization
L’Heureux, N., McAllister, T. N., & de la Fuente, L. M. (2007) · New England Journal of Medicine · doi:10.1056/nejmc071536 · Cited by 369 · cited 1×
“The field of tissue engineering has so far provided technologies that are adequate for the replacement of connective tissues such as skin [5], cartilage [6], bladder [7] and blood vessels [8].”
BackgroundHarley, B. A. C., Kim, H., Zaman, M. H., Yannas, I. V., Lauffenburger, D. A., & Gibson, L. J. (2008) · Biophysical Journal · doi:10.1529/biophysj.107.122598 · Cited by 361 · cited 2×
“One of the essential criteria to develop functional engineered tissues is to reproduce the 3D tissue architecture and provide a biologically relevant environment to the cells in order for them to maintain viability and to proliferate on the scaffolding material [20,54,55].”
BackgroundBenton, J. A., DeForest, C. A., Vivekanandan, V., & Anseth, K. S. (2009) · Tissue Engineering Part A · doi:10.1089/ten.tea.2008.0545 · Cited by 348
Micromolding of photocrosslinkable chitosan hydrogel for spheroid microarray and co-cultures
Fukuda, J., Khademhosseini, A., Yeo, Y., Yang, X., Yeh, J., Eng, G. M., Blumling, J., Wang, C.-F., Kohane, D. S., & Langer, R. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.05.044 · Cited by 334
Self-citationMann, B. K., & West, J. L. (2002) · Journal of Biomedical Materials Research · doi:10.1002/jbm.10042 · Cited by 316
Porous scaffold design using the distance field and triply periodic minimal surface models
Yoo, D. J. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.07.019 · Cited by 301 · cited 1×
“More recently, sophisticated structures have been designed by combining TPMS, distance field and optimized hexahedral elements to produce porous scaffolds having intricate internal architecture and high quality external surface [46].”
MethodTissue Engineering and Developmental Biology: Going Biomimetic
Ingber, D. E., Mow, V. C., Butler, D. L., Niklason, L. E., Huard, J., Mao, J. J., Yannas, I. V., Kaplan, D. L., & Vunjak‐Novakovic, G. (2006) · Tissue Engineering · doi:10.1089/ten.2006.12.3265 · Cited by 298 · cited 1×
“applications are designed to closely mimic the physiologic environment, including the geometrical, topographical and physical features of the targeted tissue [2,3].”
BackgroundAdvanced Tools for Tissue Engineering: Scaffolds, Bioreactors, and Signaling
Freed, L. E., Guilak, F., Guo, X. E., Gray, M. L., Tranquillo, R. T., Holmes, J. W., Radisic, M., Sefton, M. V., Kaplan, D. L., & Vunjak‐Novakovic, G. (2006) · Tissue Engineering · doi:10.1089/ten.2006.12.3285 · Cited by 284 · cited 1×
“Cell seeded porous scaffolds often display hypoxia induced cell death in their bulk due to the lack of nutrients diffusing within the 3D environment [57,58].”
BackgroundComputer-aided porous scaffold design for tissue engineering using triply periodic minimal surfaces
Yoo, D.-J. (2011) · International Journal of Precision Engineering and Manufacturing · doi:10.1007/s12541-011-0008-9 · Cited by 273 · cited 3×
“Other investigators have previously designed highly porous and biophilic structures relying on Triply Periodic Minimal Surfaces (TPMS) models [36,44,45].”
BackgroundZhou, W. Y., Lee, S. H., Wang, M., Cheung, W. L., & Ip, W. (2007) · Journal of Materials Science Materials in Medicine · doi:10.1007/s10856-007-3089-3 · Cited by 247 · cited 1×
“To produce microctructured scaffolds, multiple techniques such as solvent casting [9], particulate leaching [10,11], gas foaming [12], freeze drying [13] and more recently electrospinning [14] and laser sintering [15] have been developed.”
MethodMultilayer microfluidic PEGDA hydrogels
Cuchiara, M. P., Allen, A. C. B., Chen, T. M., Miller, J. S., & West, J. L. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.03.031 · Cited by 237 · cited 1×
“These technologies have successfully been integrated into the field of tissue engineering and have enhanced tissue functionality through control of the microenvironment [25,26].”
BackgroundDesigning synthetic materials to control stem cell phenotype
Saha, K., Pollock, J. F., Schaffer, D. V., & Healy, K. E. (2007) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2007.05.030 · Cited by 219 · cited 1×
“One of the essential criteria to develop functional engineered tissues is to reproduce the 3D tissue architecture and provide a biologically relevant environment to the cells in order for them to maintain viability and to proliferate on the scaffolding material [20,54,55].”
BackgroundChoi, J.-W., Wicker, R., Lee, S.-H., Choi, K.-H., Ha, C.-S., & Chung, I. (2009) · Journal of Materials Processing Technology · doi:10.1016/j.jmatprotec.2009.05.004 · Cited by 200
Microfabrication of Three-Dimensional Engineered Scaffolds
Borenstein, J. T., Weinberg, E. J., Orrick, B. K., Sundback, C. A., Mofrad, M. R. K., & Vacanti, J. P. (2007) · Tissue Engineering · doi:10.1089/ten.2006.0156 · Cited by 187 · cited 1×
“These studies clearly demonstrate the benefit of controlling the cellular microenvironment to reproduce the architecture of biological tissues [59].”
BackgroundFabrication of Pliable Biodegradable Polymer foams to Engineer Soft Tissues
Wake, M. C., Gupta, P. K., & Mikos, A. G. (1996) · Cell Transplantation · doi:10.1016/0963-6897(96)00025-5 · Cited by 158 · cited 1×
“To produce microctructured scaffolds, multiple techniques such as solvent casting [9], particulate leaching [10,11], gas foaming [12], freeze drying [13] and more recently electrospinning [14] and laser sintering [15] have been developed.”
MethodBiomimetic approach to tissue engineering
Grayson, W. L., Martens, T. P., Eng, G. M., Radisic, M., & Vunjak‐Novakovic, G. (2008) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2008.12.008 · Cited by 156 · cited 1×
“Cell seeded porous scaffolds often display hypoxia induced cell death in their bulk due to the lack of nutrients diffusing within the 3D environment [57,58].”
BackgroundInfrared Microscopic Imaging of Bone: Spatial Distribution of CO32−
Ou-Yang, H., Paschalis, E. P., Mayo, W. E., Boskey, A. L., & Mendelsohn, R. (2001) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.2001.16.5.893 · Cited by 151
Three‐Dimensional Polymer Constructs Exhibiting a Tunable Negative Poisson's Ratio
Fozdar, D. Y., Soman, P., Lee, J., Han, L., & Chen, S. (2011) · Advanced Functional Materials · doi:10.1002/adfm.201002022 · Cited by 150 · cited 1×
“In a recently published paper, a PSL apparatus was also used to impart negative Poisson’s ratio property to PEG hydrogel using cellular structures (pores) having special geometries, arrangements, and deformation mechanisms [53].”
MethodSelf-citationUse of a biomimetic strategy to engineer bone
Holy, C. E., Fialkov, J. A., Davies, J. E., & Shoichet, M. S. (2003) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.10453 · Cited by 139 · cited 1×
“Microscale features such as anisotropy, known to guide bone regeneration along the principal directions of porosity [16] and stiffness gradient, which can regulate stem cell differentiation [17], can be used to enhance tissue function.”
BackgroundRecent advances in tissue synthesis in vivo by use of collagen-glycosaminoglycan copolymers
Ellis, D. L., & Yannas, I. V. (1996) · Biomaterials · doi:10.1016/0142-9612(96)85567-0 · Cited by 134 · cited 1×
“To produce microctructured scaffolds, multiple techniques such as solvent casting [9], particulate leaching [10,11], gas foaming [12], freeze drying [13] and more recently electrospinning [14] and laser sintering [15] have been developed.”
MethodInterpenetrating Collagen-Fibrin Composite Matrices with Varying Protein Contents and Ratios
Rowe, S. L., & Stegemann, J. P. (2006) · Biomacromolecules · doi:10.1021/bm0602233 · Cited by 117
Val‐ala‐pro‐gly, an elastin‐derived non‐integrin ligand: Smooth muscle cell adhesion and specificity
Gobin, A. S., & West, J. L. (2003) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.10110 · Cited by 111
Projection Microfabrication of Three-Dimensional Scaffolds for Tissue Engineering
Han, L., Mapili, G., Chen, S., & Roy, K. (2008) · Journal of Manufacturing Science and Engineering · doi:10.1115/1.2823079 · Cited by 110
Self-citationMechanisms of cell activation by heavy metal ions
Wagner, M., Klein, C. L., van Kooten, T. G., & Kirkpatrick, C. J. (1998) · Journal of Biomedical Materials Research · doi:10.1002/(sici)1097-4636(19981205)42:3 · Cited by 86
Park, H., Larson, B. L., Guillemette, M. D., Jain, S. R., Hua, C. K., Engelmayr, G. C., & Freed, L. E. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.11.032 · Cited by 83
Fabrication of three-dimensional scaffolds for heterogeneous tissue engineering
Han, L., Suri, S., Schmidt, C. E., & Chen, S. (2010) · Biomedical Microdevices · doi:10.1007/s10544-010-9425-2 · Cited by 77 · cited 2×
“This PSL apparatus was previously fabricated and used to create 3D PEGbased biological scaffolds with complex internal architectures and spatial patterns in a high throughput fashion [31,40].”
MethodSelf-citation3D scaffold fabrication with PPF/DEF using micro-stereolithography
Lee, J., Lan, P. X., Kim, B. G., Lim, G., & Cho, D. (2007) · Microelectronic Engineering · doi:10.1016/j.mee.2007.01.267 · Cited by 77
Self-citationHsieh, T. M., Benjamin Ng, C. W., Narayanan, K., Wan, A. C. A., & Ying, J. Y. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.06.029 · Cited by 77 · cited 1×
“For example, scaffold fabrication using laser-microstereolithography (SL) requires a long process due to the point-by-point laser scanning procedure [38] and post-fabrication procedures [39].”
MethodWest, J. L., & Hubbell, J. A. (1995) · Biomaterials · doi:10.1016/0142-9612(95)93579-3 · Cited by 71
Effects of the architecture of tissue engineering scaffolds on cell seeding and culturing
Melchels, F. P. W., Barradas, A. M. C., van Blitterswijk, C., et al. (2010) · Acta Biomaterialia · doi:10.1016/j.actbio.2010.06.012 · Cited by 13 · cited 4×
“For example, scaffold fabrication using laser-microstereolithography (SL) requires a long process due to the point-by-point laser scanning procedure [38] and post-fabrication procedures [39].”
BackgroundMethodInfluentialCharacterization of calcium phosphate coating and zinc incorporation on the porous alumina scaffolds
Costa, H. S., Pereira, M. M., Andrade, G. I., Barbosa‐Stancioli, E. F., & Mansur, H. S. (2007) · Materials Research · doi:10.1590/s1516-14392007000100007 · Cited by 8
Flagged first, then by citations
2 of the 68 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels
Yue, K., Trujillo-de Santiago, G., Alvarez, M. M., Tamayol, A., Annabi, N., & Khademhosseini, A. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.08.045 · Cited by 3,146
“reported the use of a custom designed projection stereolithography system to fabricate GelMA hydroge ls with tunable microarchitectures (Figure 2B) [41].”
BackgroundMethodInfluentialSelf-citationModeling physiological events in 2D vs. 3D cell culture
Duval, K., Grover, H., Han, L.-H., Mou, Y., Pegoraro, A. F., Fredberg, J., & Chen, Z. (2017) · Physiology · doi:10.1152/physiol.00036.2016 · Cited by 1,849
“mechano-responses, and cell survival (2, 10, 41).”
BackgroundBone tissue engineering using 3D printing
Bose, S., Vahabzadeh, S., & Bandyopadhyay, A. K. (2013) · Materials Today · doi:10.1016/j.mattod.2013.11.017 · Cited by 1,873
3D bioprinting for engineering complex tissues
Mandrycky, C., Wang, Z., Kim, K., & Kim, D. (2015) · Biotechnology Advances · doi:10.1016/j.biotechadv.2015.12.011 · Cited by 1,818
“(A) Schematic illustration of a stereolithography system (Gauvin et al., 2012).”
BackgroundMethodInfluentialRecent advances in 3D printing of biomaterials
Chia, H. N., & Wu, B. M. (2015) · Journal of Biological Engineering · doi:10.1186/s13036-015-0001-4 · Cited by 1,734
“Stiffer structures have been studied in vitro with mouse pre-osteoblasts [70], human umbilical vein endothelial cells [78], rat bone marrow cells [73], MC3T3-E1 pre-osteoblasts [63,72,74], and human mesenchymal stem cells[71].”
Background
Metadata
- Authors
- Gauvin, R., Chen, Y.-C., Lee, J. W., Soman, P., Zorlutuna, P., Nichol, J. W., Bae, H., Chen, S., & Khademhosseini, A.
- Affiliations
- Brigham and Women's Hospital (US)
- University of California San Diego (US)
- Corresponding author
- Chen, Ying-Chieh
- Chen, Shaochen
- Khademhosseini, Ali
- ORCID
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2012-05
- Volume
- 33
- Issue
- 15
- Pages
- 3824-3834
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC3766354
- OpenAlex
- W2164279637
- Cited by
- 641
- References
- 68
- Citations by year
- 2026: 13 · 2025: 30 · 2024: 28 · 2023: 42 · 2022: 57 · 2021: 58 · 2020: 66 · 2019: 69 · 2018: 59 · 2017: 53 · 2016: 43 · 2015: 43 · 2014: 41 · 2013: 28 · 2012: 10
- FWCI
- 22.25
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (HL099073, EB012597, HL092836)
- Fonds Québécois de la Recherche sur la Nature et les Technologies
- National Science Foundation (DMR0847287)
- Office of Naval Research
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Keywords
- projection stereolithography, tissue engineering scaffolds, gelatin methacryloyl, 3D microfabrication, porous scaffolds, scaffold porosity, human umbilical vein endothelial cells, endothelial phenotype, scaffold mechanical properties, scaffold architecture
- MeSH terms
- Fluorescent Antibody Technique, Humans, Porosity, Microscopy, Confocal, Tissue Engineering, Green Fluorescent Proteins, Tissue Scaffolds, Microtechnology, Human Umbilical Vein Endothelial Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
