The DOI matches its Crossref record; 66 of the 68 references it cites were found in a registry or an index.
Microfabrication of complex porous tissue engineering scaffolds using 3D projection stereolithography
Open accessAbstract
The success of tissue engineering will rely on the ability to generate complex, cell seeded three-dimensional (3D) structures. Therefore, methods that can be used to precisely engineer the architecture and topography of scaffolding materials will represent a critical aspect of functional tissue engineering. Previous approaches for 3D scaffold fabrication based on top-down and process driven methods are often not adequate to produce complex structures due to the lack of control on scaffold architecture, porosity, and cellular interactions. The proposed projection stereolithography (PSL) platform can be used to design intricate 3D tissue scaffolds that can be engineered to mimic the microarchitecture of tissues, based on computer aided design (CAD). The PSL system was developed, programmed and optimized to fabricate 3D scaffolds using gelatin methacrylate (GelMA). Variation of the structure and prepolymer concentration enabled tailoring the mechanical properties of the scaffolds. A dynamic cell seeding method was utilized to improve the coverage of the scaffold throughout its thickness. The results demonstrated that the interconnectivity of pores allowed for uniform human umbilical vein endothelial cells (HUVECs) distribution and proliferation in the scaffolds, leading to high cell density and confluency at the end of the culture period. Moreover, immunohistochemistry results showed that cells seeded on the scaffold maintained their endothelial phenotype, demonstrating the biological functionality of the microfabricated GelMA scaffolds.
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“Results from 3D studies demonstrate that increasing the dimensionality of extracellular matrix (ECM) around cells from 2D to 3D can significantly impact cell proliferation, differentiation, mechano-responses, and cell survival (2,10,41).”
From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [41]
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Citations
Cites
Matrix elasticity directs stem cell lineage specification
Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915
Langer, R., & Vacanti, J. P. (1993) · Science · doi:10.1126/science.8493529 · Cited by 9,893
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
Discher, D. E., Janmey, P., & Wang, Y.-L. (2005) · Science · doi:10.1126/science.1116995 · Cited by 6,382 · cited 1×
“Results obtained were found to be within a physiologic range (0e800 kPa) [65] and prepolymer concentration and scaffold geometry were shown to enable the tailoring of the mechanical properties of the hydrogels.”
BackgroundLutolf, M. P., & Hubbell, J. A. (2005) · Nature Biotechnology · doi:10.1038/nbt1055 · Cited by 4,551 · cited 1×
“One of the essential criteria to develop functional engineered tissues is to reproduce the 3D tissue architecture and provide a biologically relevant environment to the cells in order for them to maintain viability and to proliferate on the scaffolding material [20,54,55].”
BackgroundPorous scaffold design for tissue engineering
Hollister, S. J. (2005) · Nature Materials · doi:10.1038/nmat1421 · Cited by 3,979 · cited 1×
“applications are designed to closely mimic the physiologic environment, including the geometrical, topographical and physical features of the targeted tissue [2,3].”
BackgroundTaking Cell-Matrix Adhesions to the Third Dimension
Cukierman, E., Pankov, R., Stevens, D. R., & Yamada, K. M. (2001) · Science · doi:10.1126/science.1064829 · Cited by 3,027 · cited 1×
“The ability to fabricate three dimensional (3D) scaffolds having specifically designed microarchitecture reproducing the in vivo environment could elicit a physiologically relevant response and result into improved functionality of the tissue [4].”
BackgroundA review on stereolithography and its applications in biomedical engineering
Melchels, F. P. W., Feijén, J., & Grijpma, D. W. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.04.050 · Cited by 2,473
Cell-laden microengineered gelatin methacrylate hydrogels
Nichol, J. W., Koshy, S. T., Bae, H., Hwang, C. M., Yamanlar, S., & Khademhosseini, A. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.03.064 · Cited by 2,377 · cited 5×
“In the present study, a custom designed projection stereolithography (PSL) system was used to control the 3D microarchitecture of collagen-based gelatin methacrylate (GelMA) hydrogels [21,43].”
BackgroundMethodInfluentialSelf-citationElectrospinning of Collagen Nanofibers
Matthews, J. A., Wnek, G. E., Simpson, D. G., & Bowlin, G. L. (2002) · Biomacromolecules · doi:10.1021/bm015533u · Cited by 2,253 · cited 1×
“To produce microctructured scaffolds, multiple techniques such as solvent casting [9], particulate leaching [10,11], gas foaming [12], freeze drying [13] and more recently electrospinning [14] and laser sintering [15] have been developed.”
MethodMicroscale technologies for tissue engineering and biology
Khademhosseini, A., Langer, R., Borenstein, J. T., & Vacanti, J. P. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0507681102 · Cited by 1,553 · cited 2×
“Scaffold properties at the microscale are known to influence cell-ECM interactions and to dictate the mechanical properties and degradation behavior of a biomaterial [25].”
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Items 601–641 of 641
Novel Approaches for Patterning Hierarchical Hydrogels
Wang, S. (2018) · Queensland University of Technology · doi:10.5204/thesis.eprints.121231
Polymers in Biofabrication and 3D Tissue Modelling
Melchels, F. P. W. (2019) · Royal Society of Chemistry eBooks · doi:10.1039/9781788012683-00119
A Short Review on Bio-compatible/Bio-degradable| Photopolymers for Stereolithography Bio-3D Printing
Nazir, A. (2018) · Trends in Technical & Scientific Research · doi:10.19080/ttsr.2018.02.555583
“DLP is another emerging SL technique in which an array of millions of independently rotatable mirrors is used to project the light in order to polymerize photosensitive materials layerby-layer under the action of UV or visible light [5].”
Manufacture of complex heart tissues: technological advancements and future directions
Zhang, Y. (2021) · AIMS bioengineering · doi:10.3934/bioeng.2021008
Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels
Szklanny, A. A., Neale, D. B., Lahann, J., & Levenberg, S. (2021) · Journal of Visualized Experiments · doi:10.3791/61995 · Cited by 1
Tailored nanotopography of photocurable composites for control of cell migration
Hasselmann, S., Kopittke, C., Götz, M., Witzel, P., Riffel, J., & Heinrich, D. (2021) · RSC Advances · doi:10.1039/d0ra06530g
A four-channel air pressure cooperative control system for 3-D bio-printing device
Wu, X., Wang, S., Wang, X., & He, G. (2021) · Journal of Computational Methods in Sciences and Engineering · doi:10.3233/jcm-204798
“At present, according to the different working principle of air pressure extrusion, 3D bio-printing devices can be roughly divided into three categories, thermal inkjet printer, acoustic inkjet printer and micro-extrusion molding bio-printer [1–3].”
Three Dimensional (3D) Printable Gel-Inks for Skin Tissue Regeneration
Nazarnezhad, S., Hooshmand, S., Baino, F., & Kargozar, S. (2021) · Gels horizons: from science to smart materials · doi:10.1007/978-981-16-4667-6_6
Microfabrication of Highly Biocompatible Materials for Energy Applications
Hashemi, N., Poursharifi, Z., Asrar, P., Montazami, R., & Hashemi, N. N. (2013) · Iowa State University Digital Repository (Iowa State University)
Evolution of Cartilage Repair Technology
Zhou, C., Chen, Q. H., Li, Z., Wang, X., Peng, J. C., & Yu, C. (2022) · IntechOpen eBooks · doi:10.5772/intechopen.108031
A Review on Textile Scaffolds – For an Effective Healing and Regeneration
Singh, S. A. S. J. V. (2023) · International Journal for Modern Trends in Science and Technology · doi:10.46501/ijmtst0902034
Shi, H., Li, Y., Xu, K., & Yin, J. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4458412
Biomimetic composite scaffolds for meniscus repair: recent progress and future outlook
Wang, R., Xie, H., & Huang, Y. (2023) · Elsevier eBooks · doi:10.1016/b978-0-323-90597-8.00018-9
Polymeric Biomaterials for Bioprinting Applications
Sonkar, A. K., Kundu, A., Sharma, D., Agarwal, V. K., & Chanda, A. (2023) · Materials horizons · doi:10.1007/978-981-99-5064-5_11
3D Photopolymerization for Biofabrication
Nottrodt, N., & Bremus‐Köbberling, E. (2023) · RWTHedition · doi:10.1007/978-3-030-98323-9_18
Flandes‐Iparraguirre, M. (2023) · Deposito Adademico Digital Universidad De Navarra (University of Navarra) · doi:10.15581/10171/69152
Towards the creation of an atlas of scaffold patterns
van Kampen, K. A. (2024) · Research Publications (Maastricht University) · doi:10.26481/dis.20240402kk
A Novel 3-D-Printed Passive Microfluidic Temperature Sensor for Medical Applications
Gmati, I. E., Gmati, Y., Amara, W., Ghayoula, R., Smida, A., Waly, M. I., Fattahi, J., & Satouri, J. (2025) · IEEE Transactions on Components Packaging and Manufacturing Technology · doi:10.1109/tcpmt.2025.3552029
Bioprinting of engineering tissue constructs in medical science: A brief cancer overview
Wilson, D. W., Buttar, H. S., & Tuli, H. S. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-22300-6.00057-7
Needless Needles: 3D Printing, Fabrication, and Delivery of Drugs and Vaccines
Mendonsa, N. S., Vaz, J. M., Eashan, V. S., & Balaji, S. (2025) · Springer eBooks · doi:10.1007/978-3-031-77911-4_10
3D-printed scaffolds using cellulose nanofibrils
Tripathy, N. S., Paikray, S. K., Sahoo, L., & Dilnawaz, F. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-26518-1.00013-6
Multidimensional liquid patterning for 3D microtissue engineering
Abira, R., Gokarn, S., & Parveen, S. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-26518-1.00005-7
Bone Organoids: Current Approaches, Challenges, and Potential Applications
Dutta, K., & Nayak, S. (2025) · BENTHAM SCIENCE PUBLISHERS eBooks · doi:10.2174/9789815238693125010006
Influence of bioengineering and bioinformatics technologies on public health status
Vidyasheva, I. V., Erugina, M. V., Arkhangelskaya, A. A., Dv, T., Strizhevskaya, V. N., Maradudin, M. S., & Романова, Х. С. (2025) · Saratov Journal of Medical Scientific Research · doi:10.15275/ssmj2102194
Shaping hydrogel bioinks into 3D, multiscale, perfusable models using multimodal printingPreprint
Kunwar, P., Poudel, A., Aryal, U., Geffert, Z. J., Fougnier, D., Narkar, A. R., Zhang, K., Filip, A., & Soman, P. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.01.29.702588
Self-citationBiocomposites électrofilés et microfabrication 3D pour l’ingénierie des tissus osseux
Bellani, C. F. (2018) · theses.fr (ABES) · doi:10.70675/4f560f95z9e10z4206zbb49z6b925f308a33
3D high-content screening imaging of organoid development using single objective SPIM
Delaire, T. (2022) · theses.fr (ABES) · doi:10.70675/af908f4fz0244z4c53z8e1azc8c96f94e7ac
Impact of flow rate in extrusion-based bioprinting to improve printing quality
Tseng, W.-C. (2025) · theses.fr (ABES) · doi:10.70675/7604f308z03fcz43a5z99aezefdd7ccb94c3
Burmas, N. C., & Smith, Q. (2026) · Biofabrication · doi:10.1088/1758-5090/ae63f8
Arifin, N., Sudin, I., & Ngadiman, N. H. A. (2026) · SpringerBriefs in applied sciences and technology · doi:10.1007/978-3-032-15470-5_16
3D‐Printed Mesh‐Walled Microtube System Enables a Vascular‐Biliary‐Incorporated Liver‐on‐a‐Chip
xue, S., Pang, Y., Song, Y., Liu, R., Xu, C., Zhang, H., Ouyang, L., Sun, W., & Lin, F. (2026) · Advanced Functional Materials · doi:10.1002/adfm.77710
Synthetic polymers in 3D bioprinting
Jadeja, P., & Patel, R. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-34207-3.00010-1
Extrusion‐Based Bioprinting of Living Bioinks for Tissue and Organ Engineering
Silberman, E., Baruch, E., Cohen, R., Shapira, A., & Dvir, T. (2026) · Advanced Materials Technologies · doi:10.1002/admt.71405
Model-Dependent EV Biomarker Signatures: From Culture Systems to Translational Perspectives
di Fazio, G., Poppa, G., D’Ascenzo, S., Dolo, V., Taverna, S., & Giusti, I. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27209325
“They can be produced through various techniques, such as 3D printing [81], stereolithography [82], freeze-drying, polymer phase separation, gas foaming [83], and porogenic materials [84].”
Putame, G., Gabetti, S., Chirio, A., & Massai, D. (2026) · Advances in chemical engineering · doi:10.1016/bs.ache.2026.09.001
Metadata
- Authors
- Gauvin, R., Chen, Y.-C., Lee, J. W., Soman, P., Zorlutuna, P., Nichol, J. W., Bae, H., Chen, S., & Khademhosseini, A.
- Affiliations
- Brigham and Women's Hospital (US)
- University of California San Diego (US)
- Corresponding author
- Chen, Ying-Chieh
- Chen, Shaochen
- Khademhosseini, Ali
- ORCID
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2012-05
- Volume
- 33
- Issue
- 15
- Pages
- 3824-3834
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC3766354
- OpenAlex
- W2164279637
- Cited by
- 641
- References
- 68
- Citations by year
- 2026: 13 · 2025: 30 · 2024: 28 · 2023: 42 · 2022: 57 · 2021: 58 · 2020: 66 · 2019: 69 · 2018: 59 · 2017: 53 · 2016: 43 · 2015: 43 · 2014: 41 · 2013: 28 · 2012: 10
- FWCI
- 22.25
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (HL099073, EB012597, HL092836)
- Fonds Québécois de la Recherche sur la Nature et les Technologies
- National Science Foundation (DMR0847287)
- Office of Naval Research
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Keywords
- projection stereolithography, tissue engineering scaffolds, gelatin methacryloyl, 3D microfabrication, porous scaffolds, scaffold porosity, human umbilical vein endothelial cells, endothelial phenotype, scaffold mechanical properties, scaffold architecture
- MeSH terms
- Fluorescent Antibody Technique, Humans, Porosity, Microscopy, Confocal, Tissue Engineering, Green Fluorescent Proteins, Tissue Scaffolds, Microtechnology, Human Umbilical Vein Endothelial Cells
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