The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Porous chitosan-hyaluronic acid scaffolds as a mimic of glioblastoma microenvironment ECM
Open accessAbstract
Cancer therapeutics are developed through extensive screening; however, many therapeutics evaluated with 2D in vitro cultures during pre-clinical trials suffer from lower efficacy in patients. Replicating the in vivo tumor microenvironment in vitro with three-dimensional (3D) porous scaffolds offers the possibility of generating more predictive pre-clinical models to enhance cancer treatment efficacy. We developed a chitosan and hyaluronic acid (HA) polyelectrolyte complex 3D porous scaffold and evaluated its physical properties. Chitosan-HA (C-HA) scaffolds had a highly porous network. C-HA scaffolds were compared to 2D surfaces for in vitro culture of U-118 MG human glioblastoma (GBM) cells. C-HA scaffold cultures promoted tumor spheroid formation and increased stem-like properties of GBM cells as evidenced by the upregulation of CD44, Nestin, Musashi-1, GFAP, and HIF-1α as compared with 2D cultures. Additionally, the invasiveness of GBM cells cultured in C-HA scaffolds was significantly enhanced compared to those grown in 2D cultures. C-HA scaffold cultures were also more resistant to chemotherapy drugs, which corresponded to the increased expression of ABCG2 drug efflux transporter. These findings suggest that C-HA scaffolds offer promise as an in vitro GBM platform for study and screening of novel cancer therapeutics.
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Cites
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“0 kPa [37], so the C-HA scaffolds have a Young’s modulus in the wet state that more closely matches the stiffness of native brain tissue than tissue culture polystyrene (Young’s modulus of w106 kPa) [38].”
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Self-citation
Cited by
Biomimetic porous scaffolds for bone tissue engineering
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Three-Dimensional Spheroids as In Vitro Preclinical Models for Cancer Research
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Metadata
- Authors
- Florczyk, S. J., Wang, K., Jana, S., Wood, D. L., Sytsma, S. K., Sham, J. G., Kievit, F. M., & Zhang, M.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2013-12
- Volume
- 34
- Issue
- 38
- Pages
- 10143-10150
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2097730243
- Cited by
- 214
- References
- 11
- FWCI
- 6.09
- Type
- journal article
- Language
- en
- Open access
- green
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