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MiRNA-124 induces neuroprotection and functional improvement after focal cerebral ischemia

Hamzei Taj, Somayyeh, Kho, Widuri, Riou, Adrien, Wiedermann, Dirk, Hoehn, Mathias·2016·Biomaterials
DOI 10.1016/j.biomaterials.2016.03.025
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Abstract

microRNA-124 (miR-124), the most abundant miRNA of the CNS, was recently shown to modulate the polarization of activated microglia and infiltrating macrophages towards the anti-inflammatory M2 phenotype and protect neurons in various ways after brain disease. In ischemic stroke, microglia and macrophages of a detrimental and persistent pro-inflammatory M1 phenotype have been shown to aggravate the secondary injury. Thus, shifting the polarization of microglia/macrophages into the beneficial, anti-inflammatory M2-like phenotype is considered neuroprotective after stroke onset. Here, we have induced 30 min transient occlusion of the right middle cerebral artery (MCAO) in 34 male, C57BL/6 mice. Lesion development was monitored with T2-weighted MRI. Liposomated miR-124 was injected in 11 animals at 48 h and in 5 animals at 10 days after MCAO. Arg-1, a marker for M2 phenotype, was co-stained with Iba-1, NeuN or GFAP. The distribution of astrocytes, neurons and microglia/macrophages and their expression of Arg-1 were quantified. Early miR-124 injection resulted in a significantly increased neuronal survival and a significantly increased number of M2-like polarized microglia/macrophages. Moreover, the lesion core, delineated by reactive astrocytes, was significantly reduced over time upon early miR-124 injection. These neuroprotective and anti-inflammatory effects of the early miR-124 treatment were pronounced during the first week with Arg-1. Number of Arg-1+ microglia/macrophages…

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Metadata

Authors
Hamzei Taj, S., Kho, W., Riou, A., Wiedermann, D., & Hoehn, M.
Venue
Biomaterials
Publisher
Elsevier BV
Published
2016-06
Volume
91
Pages
151-165
ISSN
0142-9612
DOI
10.1016/j.biomaterials.2016.03.025
Cited by
175
References
5
Language
en
Licence
Funders
EU-FP7

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CoChat. (2026). Citation check: MiRNA-124 induces neuroprotection and functional improvement after focal cerebral ischemia. https://app.cochat.ai/papers/10.1016/j.biomaterials.2016.03.025
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Hamzei Taj, S., Kho, W., Riou, A., Wiedermann, D., & Hoehn, M. (2016). MiRNA-124 induces neuroprotection and functional improvement after focal cerebral ischemia. Biomaterials. https://doi.org/10.1016/j.biomaterials.2016.03.025