Cited by 125Hydrogels: synthesis, properties, applications3D Printing in Biomedical ResearchCellular Mechanics and Interactions

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Polysaccharide matrices used in 3D in vitro cell culture systems

Diekjürgen, Dorina, Grainger, David W.·2017·Biomaterials

DOI 10.1016/j.biomaterials.2017.06.020

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Abstract

Polysaccharides comprise a diverse class of polymeric materials with a history of proven biocompatibility and continual use as biomaterials. Recent focus on new matrices appropriate for three-dimensional (3D) cell culture offers new opportunities to apply polysaccharides as extracellular matrix mimics. However, chemical and structural bases for specific cell-polysaccharide interactions essential for their utility as 3-D cell matrices are not well defined. This review describes how these naturally sourced biomaterials satisfy several key properties for current 3D cell culture needs and can also be synthetically modified or blended with additional components to tailor their cell engagement properties. Beyond their benign interactions with many cell types in cultures, their economical and high quality sourcing, optical clarity for ex situ analytical interrogation and in situ gelation represent important properties of these polymers for 3D cell culture applications. Continued diversification of their versatile glycan chemistry, new bio-synthetic sourcing strategies and elucidation of new cell-specific properties are attractive to expand the polysaccharide polymer utility for cell culture needs. Many 3D cell culture priorities are addressed with the portfolio of polysaccharide materials available and under development. This review provides a critical analysis of their properties, capabilities and challenges in 3D cell culture applications.

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Authors: Diekjürgen, D., & Grainger, D. W.
Affiliations: University of Utah (US)
Corresponding author: Grainger, David W.
ORCID: Grainger, David W.
Venue: Biomaterials
Publisher: Elsevier BV
Published: 2017-10
Volume: 141
Pages: 96-115
ISSN: 0142-9612 (ISSN-L)
DOI: 10.1016/j.biomaterials.2017.06.020
OpenAlex: W2701735813
Cited by: 125
References: 329
Citations by year: 2026: 7 · 2025: 10 · 2024: 18 · 2023: 14 · 2022: 21 · 2021: 16 · 2020: 8 · 2019: 19 · 2018: 11 · 2017: 1
FWCI: 0.69
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
Locations: Biomaterials (publishedVersion)
PubMed (publishedVersion)
Topics: Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords: polysaccharides, 3D cell culture, extracellular matrix mimics, biocompatibility, in situ gelation
MeSH terms: Animals, Biocompatible Materials, Extracellular Matrix, Glycosaminoglycans, Humans, Polysaccharides, Cell Culture Techniques, Hydrogels, Tissue Scaffolds

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Polysaccharide matrices used in 3D in vitro cell culture systems

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