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3D extracellular matrix mimics: fundamental concepts and role of materials chemistry to influence stem cell fate

Nicolas, Julien, Magli, Sofia, Rabbachin, Linda, Sampaolesi, Susanna, Nicotra, Francesco, Russo, Laura·2020·Biomacromolecules
DOI 10.1021/acs.biomac.0c00045
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Abstract

Synthetic 3D extracellular matrices (ECMs) find application in cell studies, regenerative medicine, and drug discovery. While cells cultured in a monolayer may exhibit unnatural behavior and develop very different phenotypes and genotypes than in vivo, great efforts in materials chemistry have been devoted to reproducing in vitro behavior in in vivo cell microenvironments. This requires fine-tuning the biochemical and structural actors in synthetic ECMs. This review will present the fundamentals of the ECM, cover the chemical and structural features of the scaffolds used to generate ECM mimics, discuss the nature of the signaling biomolecules required and exploited to generate bioresponsive cell microenvironments able to induce a specific cell fate, and highlight the synthetic strategies involved in creating functional 3D ECM mimics.

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  • Additional ECM components are used as coating during cultivation to enhance cell adhesion, growth, and differentiation.

    “Neuronal cells, particularly those derived from stem cells, require a supportive ECM environment that mimics their natural niche to maintain their viability, morphology, and function, which is why additional ECM components are used as coating during cultivation to enhance cell adhesion, growth, and differentiation (Friedl and Brocker, 2000; Theocharis et al., 2016; Nicolas et al., 2020).”

    From Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays (2026) · cites this paper as Nicolas et al., 2020

    Section 3 (page not provided) says ECM mimics can be used as surface coatings to promote cell adhesion. Section 2.1.2 says laminin is used as a coating to induce specific cell fates and functions and notes its role in neurite outgrowth. The passages do not establish that coatings enhance growth or that stem-cell-derived neuronal cells generally require such coatings to maintain viability, morphology, and function. (LLM, conf=0.82)

    Experimental — accuracy not yet measured

  • Neuronal cells, particularly those derived from stem cells, require a supportive ECM environment that mimics their natural niche to maintain their viability, morphology, and function.

    “Neuronal cells, particularly those derived from stem cells, require a supportive ECM environment that mimics their natural niche to maintain their viability, morphology, and function, which is why additional ECM components are used as coating during cultivation to enhance cell adhesion, growth, and differentiation (Friedl and Brocker, 2000; Theocharis et al., 2016; Nicolas et al., 2020).”

    From Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays (2026) · cites this paper as Nicolas et al., 2020

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200 of 389 shown

Cited by

Sorted by citations

Authors
Nicolas, J., Magli, S., Rabbachin, L., Sampaolesi, S., Nicotra, F., & Russo, L.
Affiliations
  • Institut Galien Paris-Saclay , , , ,
  • University of Milano-Bicocca , , Piazza della Scienza 2 , ,
ORCID
Venue
Biomacromolecules
Publisher
American Chemical Society (ACS)
Published
2020-03-31
Volume
21
Issue
6
Pages
1968-1994
ISSN
  • 1525-7797
  • 1526-4602
DOI
10.1021/acs.biomac.0c00045
Cited by
504
References
16
Language
en
Licence
pubs.acs.org/page/policy/authorchoice_termsofuse.html (vor, 17 d)
Funders
  • H2020 Industrial Leadership (760986)
  • Ministero della Salute (RF-2016-02362946)
  • Regione Lombardia (NA)

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