The DOI matches its Crossref record; 75 of the 75 references it cites were found in a registry or an index.
Hydrogels derived from decellularized liver tissue support the growth and differentiation of cholangiocyte organoids
Open accessAbstract
Human cholangiocyte organoids are promising for regenerative medicine applications, such as repair of damaged bile ducts. However, organoids are typically cultured in mouse tumor-derived basement membrane extracts (BME), which is poorly defined, highly variable and limits the direct clinical applications of organoids in patients. Extracellular matrix (ECM)-derived hydrogels prepared from decellularized human or porcine livers are attractive alternative culture substrates. Here, the culture and expansion of human cholangiocyte organoids in liver ECM(LECM)-derived hydrogels is described. These hydrogels support proliferation of cholangiocyte organoids and maintain the cholangiocyte-like phenotype. The use of LECM hydrogels does not significantly alter the expression of selected genes or proteins, such as the cholangiocyte marker cytokeratin-7, and no species-specific effect is found between human or porcine LECM hydrogels. Proliferation rates of organoids cultured in LECM hydrogels are lower, but the differentiation capacity of the cholangiocyte organoids towards hepatocyte-like cells is not altered by the presence of tissue-specific ECM components. Moreover, human LECM extracts support the expansion of ICO in a dynamic culture set up without the need for laborious static culture of organoids in hydrogel domes. Liver ECM hydrogels can successfully replace tumor-derived BME and can potentially unlock the full clinical potential of human cholangiocyte organoids.
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“Rheological data always showed storage modulus (G′) values higher than loss modulus (G″) values, typical stable gel behavior.”
In the citing paper
“G′ values quoted ranged between ∼100 Pa and in excess of 5000 Pa and depended on the concentration of the ECM (usually 5–20 mg/mL) and the crosslinking method [23,24].”
In the citing paper
“G′ values quoted ranged between ∼100 Pa and in excess of 5000 Pa and depended on the concentration of the ECM (usually 5–20 mg/mL) and the crosslinking method [23,24].”
In the citing paper
“Turbide measurements indicated a concentration-dependent initial lag followed by the formation of increasing turbide, characteristic of collagen fibrillogenesis [23].”
In the citing paper
“In addition, dLECM has been shown to have the potential as a better-than-Matrigel alternative culture system for organoid cultures that provides a xenogeneic-free, tissue-specific niche that supports the growth of cholangiocyte and hepatocyte organoids without the risk profile seen with tumor-derived matrices [23].”
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Items 101–107 of 107
Hepatobiliary organoids and large-scale expansion for regenerative therapies
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Developing a Clinically Practical Biomaterial Platform for Endogenous Liver Regeneration
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Metadata
- Authors
- Willemse, J., van Tienderen, G., van Hengel, E., Schurink, I., van der Ven, D., Kan, Y., de Ruiter, P., Rosmark, O., Westergren-Thorsson G, G., Schneeberger, K., van der Eerden, B., Roest, H., Spee, B., van der Laan, L., de Jonge, J., & Verstegen, M.
- Affiliations
- Erasmus MC (NL)
- Lund University (SE)
- Utrecht University (NL)
- Corresponding author
- Verstegen, Monique
- ORCID
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2022-05
- Volume
- 284
- Pages
- 121473
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W4220784575
- Cited by
- 106
- References
- 75
- Citations by year
- 2026: 9 · 2025: 37 · 2024: 32 · 2023: 22 · 2022: 6
- FWCI
- 23.16
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Biomaterials (publishedVersion, cc-by)
- PubMed (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (publishedVersion, cc-by)
- EUR Research Repository (Erasmus University Rotterdam) (submittedVersion, cc-by)
- Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
- Lund University Publications (Lund University) (submittedVersion)
- Funders
- University of Twente
- Medical Delta
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Keywords
- cholangiocyte organoids, hydrogels, bile duct regeneration, CK7, regenerative medicine
- MeSH terms
- Animals, Extracellular Matrix, Humans, Liver, Neoplasms, Organoids, Plant Extracts, Swine, Hydrogels, Mice
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