Experimental diabetes induced by streptozotocin in the desert gerbil, Gerbillus gerbillus, and the effects of short-term 20-hydroxyecdysone administration
Abstract
The present work was aimed at studying the effects of streptozotocin (STZ; 130 mg/kg) in the desert gerbil, Gerbillus gerbillus, and at evaluating the impact of the short-term administration of 20-hydroxyecdysone (20E; 5 mg/kg). We observed that administration of streptozotocin caused a significant increase in plasmatic glucose and a decrease in insulin levels. The plasma lipid profile and liver glycogen content were also altered. The activities of antioxidant enzymes and malondialdehyde (MDA) levels were increased in the pancreatic tissue of STZ-treated gerbils. Moreover, histopathological and immunohistochemical analysis showed degenerative damage in the pancreas with a decline in the percentage area of β-cells. Treatment of STZ-treated gerbils with 20E counteracted metabolic disorders and reduced lipid peroxidation. Histological and immunohistochemical studies showed moderate amelioration in the pancreatic structure. These findings indicate that streptozotocin administration induced experimental diabetes in gerbils and that short-term administration of 20E has beneficial effects in glucose homeostasis in STZ-treated gerbils suggesting that 20E may stimulate surviving β-cells to release more insulin.
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In streptozotocin-induced diabetes of desert gerbil, 20E increased glycogen store, reduced lipid peroxidation, and counteracted metabolic disorders.
“The glycogen store was also increased, lipid peroxidation was reduced, and metabolic disorders were counteracted by 20E.”
From Molecular Targets of 20-Hydroxyecdysone in Mammals, Mechanism of Action: Is It a Calorie Restriction Mimetic and Anti-Aging Compound? (2025) · cites this paper as [69]
A single 20E administration 3 h before sacrifice was enough to ameliorate plasma insulin and glucose and moderate degranulation of beta cells of pancreatic islets of Langerhans in streptozotocin-induced diabetic gerbil.
“Interestingly, 20E administered only once, 3 h before animal sacrificing, was enough to ameliorate plasma levels of insulin and glucose and moderate degranulation of beta cells of pancreatic islets of Langerhans [69].”
From Molecular Targets of 20-Hydroxyecdysone in Mammals, Mechanism of Action: Is It a Calorie Restriction Mimetic and Anti-Aging Compound? (2025) · cites this paper as [69]
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“…lipid peroxidation was reduced, and metabolic disorders were counteracted by 20E. Interestingly, 20E administered only once, 3 h before animal sacrificing, was enough to ameliorate plasma levels of insulin and glucose and moderate degranulation of beta cells of pancreatic islets of Langerhans [69].”
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Self-citation
- Authors
- Mallek, A., Movassat, J., Ameddah, S., Liu, J., Semiane, N., Khalkhal, A., & Dahmani, Y.
- Affiliations
- University of Sciences and Technology Houari Boumediene (DZ)
- Centre National de la Recherche Scientifique (FR)
- University Frères Mentouri Constantine 1 (DZ)
- Corresponding author
- Mallek, Aicha
- Venue
- Biomedicine & Pharmacotherapy
- Publisher
- Elsevier BV
- Published
- 2018-06
- Volume
- 102
- Pages
- 354-361
- ISSN
- 0753-3322 (ISSN-L)
- OpenAlex
- W2790961567
- Cited by
- 20
- References
- 43
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 2 · 2023: 4 · 2022: 3 · 2020: 1 · 2019: 1 · 2018: 1
- FWCI
- 0.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Biomedicine & Pharmacotherapy (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Ministry of Higher Education and Scientific Research
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Natural Antidiabetic Agents Studies
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
- Keywords
- streptozotocin-induced diabetes, 20-hydroxyecdysone, Gerbillus gerbillus, desert gerbil, insulin levels, plasma glucose, lipid peroxidation, antioxidant enzymes, malondialdehyde, liver glycogen, plasma lipid profile, glucose homeostasis, ecdysone
- MeSH terms
- Animals, Antioxidants, Blood Glucose, Diabetes Mellitus, Experimental, Ecdysterone, Gerbillinae, Glycogen, Insulin, Lipids, Liver, Male, Pancreas, Streptozocin, Lipid Peroxidation, Lipid Metabolism
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