Mutate Chlorella sp. by nuclear irradiation to fix high concentrations of CO2
Abstract
To improve biomass productivity and CO2 fixation of microalgae under 15% (v/v) CO2 of flue gas, Chlorella species were mutated by nuclear irradiation and domesticated with high concentrations of CO2. The biomass yield of Chlorella pyrenoidosa mutated using 500 Gy of (60)Co γ irradiation increased by 53.1% (to 1.12 g L(-1)) under air bubbling. The mutants were domesticated with gradually increased high concentrations of CO2 [from 0.038% (v/v) to 15% (v/v)], which increased the biomass yield to 2.41 g L(-1). When light transmission and culture mixing in photo-bioreactors were enhanced at 15% (v/v) CO2, the peak growth rate of the domesticated mutant (named Chlorella PY-ZU1) was increased to 0.68 g L(-1) d(-1). When the ratio of gas flow rate (L min(-1)) to 1L of microalgae culture was 0.011, the peak CO2 fixation rate and the efficiency of Chlorella PY-ZU1 were 1.54 g L(-1) d(-1) and 32.7%, respectively.
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Algal photobioreactor carbon fixation efficiency is being improved by generating mutants using gamma irradiation and optimizing growth conditions such as nitrogen, phosphorus, and magnesium levels.
“Algal (e.g., Chlorella) photobioreactor carbon fixation efficiency is currently being improved by generating various mutants using gamma irradiation and optimizing growth conditions such as nitrogen, phosphorus, and magnesium levels [95,96].”
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“, Chlorella) photobioreactor carbon fixation efficiency is currently being improved by generating various mutants using gamma irradiation and optimizing growth conditions such as nitrogen, phosphorus, and magnesium levels [95,96].”
BackgroundEnhancement of lipid production of Chlorella sp. 042 by mutagenesis
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- Authors
- Cheng, J., Huang, Y., Feng, J., Sun, J., Zhou, J., & Cen, K.
- Affiliations
- State Key Laboratory of Clean Energy Utilization (CN)
- Corresponding author
- Cheng, Jun
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2013-05
- Volume
- 136
- Pages
- 496-501
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2078333803
- Cited by
- 166
- References
- 27
- Citations by year
- 2026: 7 · 2025: 6 · 2024: 10 · 2023: 13 · 2022: 11 · 2021: 12 · 2020: 22 · 2019: 15 · 2018: 13 · 2017: 10 · 2016: 21 · 2015: 11 · 2014: 11 · 2013: 4
- FWCI
- 5.69
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Engineering › Mechanical Engineering › Carbon Dioxide Capture Technologies
- Keywords
- Chlorella pyrenoidosa, nuclear mutations, gamma irradiation, CO2 fixation, elevated CO2, flue gas, microalgae productivity, mutant selection, photobioreactors, gas flow rate, growth rate
- MeSH terms
- Carbon Dioxide, Cell Nucleus, Chlorella, Cobalt Radioisotopes, Dose-Response Relationship, Radiation, Gamma Rays, Hydrogen-Ion Concentration, Mutation, Biomass, Bioreactors, Carbon Cycle
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