Cited by 166Algal biology and biofuel productionMicrobial Metabolic Engineering and BioproductionCarbon Dioxide Capture Technologies

Mutate Chlorella sp. by nuclear irradiation to fix high concentrations of CO2

Cheng, Jun, Huang, Yun, Feng, Jia, Sun, Jing, Zhou, Junhu, Cen, Kefa·2013·Bioresource Technology

Abstract

To improve biomass productivity and CO2 fixation of microalgae under 15% (v/v) CO2 of flue gas, Chlorella species were mutated by nuclear irradiation and domesticated with high concentrations of CO2. The biomass yield of Chlorella pyrenoidosa mutated using 500 Gy of (60)Co γ irradiation increased by 53.1% (to 1.12 g L(-1)) under air bubbling. The mutants were domesticated with gradually increased high concentrations of CO2 [from 0.038% (v/v) to 15% (v/v)], which increased the biomass yield to 2.41 g L(-1). When light transmission and culture mixing in photo-bioreactors were enhanced at 15% (v/v) CO2, the peak growth rate of the domesticated mutant (named Chlorella PY-ZU1) was increased to 0.68 g L(-1) d(-1). When the ratio of gas flow rate (L min(-1)) to 1L of microalgae culture was 0.011, the peak CO2 fixation rate and the efficiency of Chlorella PY-ZU1 were 1.54 g L(-1) d(-1) and 32.7%, respectively.

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Crossref · 10.1016/j.biortech.2013.03.072

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Authors
Cheng, J., Huang, Y., Feng, J., Sun, J., Zhou, J., & Cen, K.
Affiliations
State Key Laboratory of Clean Energy Utilization (CN)
Corresponding author
Cheng, Jun
Venue
Bioresource Technology
Publisher
Elsevier BV
Published
2013-05
Volume
136
Pages
496-501
ISSN
0960-8524 (ISSN-L)
OpenAlex
W2078333803
Cited by
166
References
27
Citations by year
2026: 7 · 2025: 6 · 2024: 10 · 2023: 13 · 2022: 11 · 2021: 12 · 2020: 22 · 2019: 15 · 2018: 13 · 2017: 10 · 2016: 21 · 2015: 11 · 2014: 11 · 2013: 4
FWCI
5.69
Type
journal article
Language
en
Open access
closed
Locations
  • Bioresource Technology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Physical Sciences › Engineering › Mechanical Engineering › Carbon Dioxide Capture Technologies
Keywords
Chlorella pyrenoidosa, nuclear mutations, gamma irradiation, CO2 fixation, elevated CO2, flue gas, microalgae productivity, mutant selection, photobioreactors, gas flow rate, growth rate
MeSH terms
Carbon Dioxide, Cell Nucleus, Chlorella, Cobalt Radioisotopes, Dose-Response Relationship, Radiation, Gamma Rays, Hydrogen-Ion Concentration, Mutation, Biomass, Bioreactors, Carbon Cycle

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