Enhancing growth rate and lipid yield of Chlorella with nuclear irradiation under high salt and CO2 stress
Abstract
In order to produce biodiesel from microalgae cultured with abundant seawater, Chlorella sp. was mutated with (137)Se-γ ray irradiation and domesticated with f/2 seawater culture medium (salinity=3 wt.%) under 15 vol.% CO2 stress. Biomass yield of the mutant increased by 25% compared with wild species and lipid content increased to 54.9%. When nitrogen and phosphorus concentrations in the initial substrate increased, the increased propagation speed of the mutant resulted in decreased cell diameter by 26.6% and decreased cell wall thickness by 69.7%. The dramatically increased biomass yield of the mutant with sufficient initial substrate and relative nitrogen starvation in the later growth period with continuous 15 vol.% CO2 led to an increased lipid yield of 1.0 g/L. The long-chain unsaturated fatty acids increased, whereas short-chain saturated fatty acids decreased.
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The production of enzymes involved in desaturation and elongation reactions is reduced at nitrogen-limited condition, leading to a decrease in PUFA production.
“It is also possible that the production of enzymes involved in desaturation and elongation reactions is reduced at nitrogen-limited condition, leading to a decrease in PUFA production [118].”
From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [118]
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- Authors
- Cheng, J., Lu, H., Huang, Y., Li, K., Huang, R., Zhou, J., & Cen, K.
- Affiliations
- State Key Laboratory of Clean Energy Utilization (CN)
- Corresponding author
- Cheng, Jun
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2016-03
- Volume
- 203
- Pages
- 220-227
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2221756147
- Cited by
- 91
- References
- 31
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 5 · 2023: 12 · 2022: 12 · 2021: 16 · 2020: 18 · 2019: 7 · 2018: 7 · 2017: 6 · 2016: 2
- FWCI
- 1.48
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation (51176163, 51476141)
- Zhejiang Provincial Natural Science Foundation (LR14E060002)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
- Keywords
- Chlorella, high salinity stress, microalgal lipid production, biodiesel feedstock, seawater culture, biomass yield, cell wall thickness, nitrogen starvation, fatty acid composition, long-chain unsaturated fatty acids
- MeSH terms
- Carbon Dioxide, Cesium Radioisotopes, Chlorella, Fatty Acids, Gamma Rays, Lipids, Nitrogen, Phosphorus, Sodium Chloride, Mutagenesis, Gene Expression Regulation, Plant, Lipid Metabolism, Biofuels, Microalgae
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