Neuronal pentraxin receptor in cerebrospinal fluid as a potential biomarker for neurodegenerative diseases
Abstract
Neurodegenerative diseases, such as Alzheimer's disease (AD) and Parkinson's disease (PD), are characterized by progressive loss of cognitive function, dementia, and problems with movements. In order to find new protein biomarkers of high specificity from cerebrospinal fluid (CSF) of AD and PD patients, one-dimensional gel electrophoresis (1-DE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) as well as 2-DE analysis were performed. In 1-DE and LC-MS/MS 371 proteins were identified, among which levels of proteins such as isoform 1 of contactin-1, contactin-2, carnosine dipeptidase 1 (CNDP1), 120 kDa isoform precursor of neural cell adhesion molecule 1 (NCAM-120), alpha-dystroglycan, secreted protein acidic and rich in cysteine-like protein 1 precursor (SPARCL1), isoform 2 of calsyntenin 1 (CLSTN1), and neuronal pentraxin receptor (NPR) showed significant changes in AD or PD CSF compared with normal subjects. In 2-DE analysis approximately 747-915 spots were detected in CSF of AD or PD patients, from which 17-24 proteins with more than a 1.2 fold change were identified by tandem MS. Most proteins identified showed consistent changes in LC-MS/MS and 2-DE analysis. Three proteins that showed significant changes were selected for further validation by Western blot analysis. While NCAM-120 and alpha-dystroglycan exhibited higher levels in both AD and PD CSF compared with normal subjects, the level of NPR was increased only in AD CSF in Western blot analysis. The results were consistent with quantitative analysis of 2-DE spots. A higher level of NPR was also found in AD serum. This study suggests that NCAM-120, alpha-dystroglycan, and NPR are candidate biomarkers in CSF for neurodegenerative diseases, and that the changes in the CSF level of NPR may be specific for AD.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A study using Western Blot detected NPTXR in serum, and the values described the same trend as those obtained from CSF samples.
“A study performed using WB was able to detect NPTXR in serum, and the values described the same trend as those obtained from CSF samples (Yin et al. 2009).”
From Neuronal pentraxins as biomarkers of synaptic activity: from physiological functions to pathological changes in neurodegeneration (2022) · cites this paper as Yin et al. 2009
Cites
McKhann, G., Drachman, D., Folstein, M., Katzman, R., Price, D., & Stadlan, E. M. (1984) · Neurology · doi:10.1212/wnl.34.7.939 · Cited by 27,915 · see citing works
Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels.
Shevchenko, A., Wilm, M., Vorm, O., & Mann, M. F. (1996) · Analytical Chemistry · doi:10.1021/ac950914h · Cited by 9,047 · see citing works
Global prevalence of dementia: a Delphi consensus study
Ferri, C. P., Prince, M. J., Brayne, C., Brodaty, H., Fratiglioni, L., Ganguli, M., Hall, K. S., Hasegawa, K., Hendrie, H. C., Huang, Y., et al. (2005) · The Lancet · doi:10.1016/s0140-6736(05)67889-0 · Cited by 5,336 · see citing works
Diagnostic Criteria for Parkinson Disease
Gelb, D. J., Oliver, E., & Gilman, S. (1999) · Archives of Neurology · doi:10.1001/archneur.56.1.33 · Cited by 2,822 · see citing works
Practice parameter: Management of dementia (an evidence-based review) [RETIRED]
Doody, R. S., Stevens, J. E., Beck, C., Dubinsky, R., Kaye, J. A., Gwyther, L. P., Mohs, R. C., Thal, L. J., Whitehouse, P. J., DeKosky, S. T., et al. (2001) · Neurology · doi:10.1212/wnl.56.9.1154 · Cited by 1,703 · see citing works
Knopman, D. S., DeKosky, S. T., Cummings, J. L., Chui, H., Corey–Bloom, J., Relkin, N., Small, G. W., Miller, B., & Stevens, J. C. (2001) · Neurology · doi:10.1212/wnl.56.9.1143 · Cited by 1,410 · see citing works
ETIOLOGY AND PATHOGENESIS OF PARKINSON'S DISEASE
Olanow, C. W., & Tatton, W. G. (1999) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.22.1.123 · Cited by 1,394 · see citing works
A role for the dystrophin-glycoprotein complex as a transmembrane linker between laminin and actin
Ervasti, J. M., & Campbell, K. P. (1993) · The Journal of Cell Biology · doi:10.1083/jcb.122.4.809 · Cited by 1,388 · see citing works
Alzheimer's Disease and Parkinson's Disease
Nussbaum, R. L., & Ellis, C. E. (2003) · New England Journal of Medicine · doi:10.1056/nejm2003ra020003 · Cited by 1,351 · see citing works
Classification and prediction of clinical Alzheimer's diagnosis based on plasma signaling proteins
Ray, S., Britschgi, M., Herbert, C., Takeda-Uchimura, Y., Boxer, A., Blennow, K., Friedman, L. F., Galasko, D. R., Jutel, M., Karydas, A., et al. (2007) · Nature Medicine · doi:10.1038/nm1653 · Cited by 1,048 · see citing works
Neurodegenerative diseases: a decade of discoveries paves the way for therapeutic breakthroughs
Forman, M. S., Trojanowski, J. Q., & Lee, V. M.-Y. (2004) · Nature Medicine · doi:10.1038/nm1113 · Cited by 715 · see citing works
Oh, P., Li, Y., Yu, J., Dürr, E., Krasinska, K. M., Carver, L. A., Testa, J. E., & Schnitzer, J. E. (2004) · Nature · doi:10.1038/nature02580 · Cited by 475 · see citing works
Abdi, F. A., Quinn, J. F., Jankovic, J. J., McIntosh, M. W., Leverenz, J. B., Peskind, E. R., Nixon, R., Nutt, J. G., Chung, K., Zabetian, C. P., et al. (2006) · Journal of Alzheimer s Disease · doi:10.3233/jad-2006-9309 · Cited by 385 · see citing works
Barbeau, D., Liang, J., Robitalille, Y., Quirion, R., & Srivastava, L. K. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.7.2785 · Cited by 333 · see citing works
Neural cell adhesion molecules in activity-dependent development and synaptic plasticity
Fields, R. D., & Itoh, K. (1996) · Trends in Neurosciences · doi:10.1016/s0166-2236(96)30013-1 · Cited by 281 · see citing works
The complexities of dystroglycan
Winder, S. J. (2001) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(00)01731-x · Cited by 250 · see citing works
Sia, G.-M., Béïque, J.-C., Rumbaugh, G., Cho, R., Worley, P. F., & Huganir, R. L. (2007) · Neuron · doi:10.1016/j.neuron.2007.06.020 · Cited by 201 · see citing works
Cerebrospinal fluid proteomic biomarkers for Alzheimer's disease
Finehout, E. J., Franck, Z., Choe, L. H., Relkin, N. R., & Lee, K. H. (2006) · Annals of Neurology · doi:10.1002/ana.21038 · Cited by 185 · see citing works
Quantitative proteomics of cerebrospinal fluid from patients with Alzheimer disease
Zhang, J., Goodlett, D. R., Quinn, J. F., Peskind, E. R., Kaye, J. A., Zhou, Y. F., Pan, C., Yi, E. C., Eng, J. K., Wang, Q., et al. (2005) · Journal of Alzheimer s Disease · doi:10.3233/jad-2005-7205 · Cited by 174 · see citing works
The role of cell adhesion molecules in synaptic plasticity and memory
Murase, S., & Schuman, E. M. (1999) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(99)00019-8 · Cited by 172 · see citing works
Dodds, D. C., Omeis, I. A., Cushman, S. J., Helms, J. A., & Perin, M. S. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.34.21488 · Cited by 170 · see citing works
Salle, G. L. G. L., Rougon, G., & Valin, A. (1992) · Journal of Neuroscience · doi:10.1523/jneurosci.12-03-00872.1992 · Cited by 164 · see citing works
Biochemical Interactions of the Neuronal Pentraxins
Kirkpatrick, L., Matzuk, M. M., Dodds, D. C., & Perin, M. S. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m002254200 · Cited by 149 · see citing works
Cingulate cortex synaptic terminal proteins and neural cell adhesion molecule in schizophrenia
Honer, W. G., Falkai, P., Young, C., Wang, T., Xie, J., Bonner, J., Hu, L., Boulianne, G. L., Luo, Z., & Trimble, W. S. (1997) · Neuroscience · doi:10.1016/s0306-4522(96)00489-7 · Cited by 144 · see citing works
Breese, C. R., Freedman, R., & Leonard, S. S. (1995) · Brain Research · doi:10.1016/0006-8993(94)01384-t · Cited by 134 · see citing works
Cho, R. W., Park, J. M., Wolff, S. B. E., Xu, D., Hopf, C., Kim, J.-A., Reddy, R. C., Petralia, R. S., Perin, M. S., Linden, D. J., et al. (2008) · Neuron · doi:10.1016/j.neuron.2008.01.010 · Cited by 125 · see citing works
Murray, B. A., Hemperly, J. J., Prediger, E. A., et al. (1986) · The Journal of Cell Biology · doi:10.1083/jcb.102.1.189 · Cited by 122 · see citing works
Brain-specific proteins in cerebrospinal fluid for the diagnosis of neurodegenerative diseases
Verbeek, M., de Jong, D., & Kremer, H. (2003) · Annals of Clinical Biochemistry · doi:10.1258/000456303321016141 · Cited by 116 · see citing works
Abad, M. A., Enguita, M., DeGregorio-Rocasolano, N., Ferrer, I., & Trullas, R. (2006) · The Journal of Neuroscience · doi:10.1523/jneurosci.0575-06.2006 · Cited by 102 · see citing works
Półtorak, M., Hemperly, J. J., Williams, J. R., El‐Mallakh, R. S., & Freed, W. J. (1995) · Experimental Neurology · doi:10.1016/0014-4886(95)90048-9 · Cited by 99 · see citing works
Strekalova, H., Buhmann, C., KLEENE, R. B., Eggers, C., Saffell, J. L., Hemperly, J. J., Weiller, C., Müller‐Thomsen, T., & Schachner, M. (2005) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2004.11.013 · Cited by 98 · see citing works
Proteomic analysis of the cerebrospinal fluid of patients with schizophrenia
Jiang, L., Lindpaintner, K., Li, H.-F., Gu, N.-F., Langen, H., He, L., & Fountoulakis, M. (2003) · Amino Acids · doi:10.1007/s00726-003-0356-6 · Cited by 96 · see citing works
The Use of Proteomics in Biomarker Discovery in Neurodegenerative Diseases
Davidsson, P., & Sjögren, M. J. C. (2005) · Disease Markers · doi:10.1155/2005/848676 · Cited by 90 · see citing works
Abnormal Expression of Cell Recognition Molecules in Schizophrenia
Vawter, M. P., Cannon-Spoor, H. E., Hemperly, J. J., Hyde, T. M., VanderPutten, D. M., Kleinman, J. E., & Freed, W. J. (1998) · Experimental Neurology · doi:10.1006/exnr.1997.6721 · Cited by 84 · see citing works
Morphological properties of intracellularly labeled layer I neurons in rat neocortex
Zhou, F.-M., & Hablitz, J. J. (1996) · The Journal of Comparative Neurology · doi:10.1002/(sici)1096-9861(19961209)376:2 · Cited by 79 · see citing works
Ceruoplasmin Is Increased in Cerebrospinal Fluid in Alzheimerʼs Disease but Not Parkinsonʼs Disease
Loeffler, D. A., DeMaggio, A. J., Juneau, P. L., Brickman, C. M., Mashour, G. A., Finkelman, J., Pomara, N., & LeWitt, P. A. (1994) · Alzheimer Disease & Associated Disorders · doi:10.1097/00002093-199408030-00005 · Cited by 78 · see citing works
Increased Neural Cell Adhesion Molecule in the CSF of Patients with Mood Disorder
Półtorak, M., Frye, M. A., Wright, R., Hemperly, J. J., George, M. S., Pazzaglia, P. J., Jerrels, S., Post, R. M., & Freed, W. J. (1996) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1996.66041532.x · Cited by 78 · see citing works
Bastmeyer, M., Schlosshauer, B., & Stuermer, C. A. O. (1990) · Development · doi:10.1242/dev.108.2.299 · Cited by 64 · see citing works
Further Studies of Elevated Cerebrospinal Fluid Neuronal Cell Adhesion Molecule in Schizophrenia
van Kammen, D. P., Półtorak, M., Kelley, M. E., et al. (1998) · Biological Psychiatry · doi:10.1016/s0006-3223(97)00324-7 · Cited by 64 · see citing works
Neurotoxicity of microglial cathepsin D revealed by secretome analysis
Kim, S., Ock, J., Kim, A. K., Lee, H., Cho, J., Kim, D. R., Park, J., & Suk, K. (2007) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2007.04995.x · Cited by 63 · see citing works
Self-citationProteomic biomarker discovery in cerebrospinal fluid for neurodegenerative diseases
Zhang, J., Goodlett, D. R., & Montine, T. J. (2006) · Journal of Alzheimer s Disease · doi:10.3233/jad-2005-8407 · Cited by 58 · see citing works
Hansson, S., Puchades, M. A., Blennow, K., et al. (2004) · Proteome Science · doi:10.1186/1477-5956-2-7 · Cited by 53 · see citing works
Characterization of Mouse Spleen Cells by Subtractive Proteomics
Dieguez-Acuña, F. J., Gerber, S. A., Kodama, S., et al. (2005) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m500137-mcp200 · Cited by 47 · see citing works
Proteome Survey of Proliferating and Differentiating Rat RPE-J Cells
West, K. A., Yan, L., Miyagi, M., Crabb, J. S., Marmorstein, A. D., Marmorstein, L. Y., & Crabb, J. W. (2001) · Experimental Eye Research · doi:10.1006/exer.2001.1058 · Cited by 45 · see citing works
Altered expression of alpha-dystroglycan subunit in human gliomas
Calogero, A., Pavoni, E., Gramaglia, T., d’Amati, G., Ragona, G., Brancaccio, A., & Petrucci, T. C. (2006) · Cancer Biology & Therapy · doi:10.4161/cbt.5.4.2546 · Cited by 33 · see citing works
Dystroglycan: a multifunctional adaptor protein
Higginson, J. R., & Winder, S. J. (2005) · Biochemical Society Transactions · doi:10.1042/bst20051254 · Cited by 30 · see citing works
A function for dystroglycan in pronephros development in Xenopus laevis
Bello, V., Sirour, C., Moreau, N., Denker, E., & Darribère, T. (2008) · Developmental Biology · doi:10.1016/j.ydbio.2008.02.024 · Cited by 20 · see citing works
Son, E., Jeong, J., Lee, J., et al. (2005) · Biochemical Pharmacology · doi:10.1016/j.bcp.2005.05.027 · Cited by 20 · see citing works
Suk, K. (2006) · Expert Opinion on Therapeutic Patents · doi:10.1517/13543776.16.3.237 · Cited by 6 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Organ aging signatures in the plasma proteome track health and disease
Oh, H. S.-H., Rutledge, J., Nachun, D., Pálovics, R., Abiose, O., Moran-Losada, P., Channappa, D., Urey, D. Y., Kim, K., Sung, Y. J., et al. (2023) · Nature · doi:10.1038/s41586-023-06802-1 · Cited by 634 · see citing works
Sathe, G., Na, C. H., Renuse, S., Madugundu, A. K., Albert, M., Moghekar, A., & Pandey, A. (2019) · PROTEOMICS – Clinical Applications · doi:10.1002/prca.201800105 · Cited by 126 · see citing works
Ringman, J. M. (2012) · Archives of Neurology · doi:10.1001/archneurol.2011.642 · Cited by 124 · see citing works
Cell adhesion and matricellular support by astrocytes of the tripartite synapse
Hillen, A. E. J., Burbach, J. P. H., & Hol, E. M. (2018) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2018.02.002 · Cited by 108 · see citing works
Gómez de San José, N., Massa, F., Halbgebauer, S., Oeckl, P., Steinacker, P., & Otto, M. (2022) · Journal of Neural Transmission · doi:10.1007/s00702-021-02411-2 · Cited by 112 · see citing works
Neuronal pentraxin 2: a synapse-derived CSF biomarker in genetic frontotemporal dementia
van der Ende, E. L., Xiao, M., Xu, D., Poos, J. M., Panman, J. L., Jiskoot, L. C., Meeter, L. H., Dopper, E. G., Papma, J. M., Heller, C., et al. (2020) · Journal of Neurology, Neurosurgery & Psychiatry · doi:10.1136/jnnp-2019-322493 · Cited by 94 · see citing works
Begcevic, I., Brinc, D., Brown, M., Martinez-Morillo, E., Goldhardt, O., Grimmer, T., Magdolen, V., Batruch, I., & Diamandis, E. P. (2018) · Journal of Proteomics · doi:10.1016/j.jprot.2018.04.027 · Cited by 81 · see citing works
Cerebrospinal fluid proteome comparison between multiple sclerosis patients and controls
Kroksveen, A. C., Guldbrandsen, A., Vedeler, C., Myhr, K. M., Opsahl, J. A., & Berven, F. S. (2012) · Acta Neurologica Scandinavica · doi:10.1111/ane.12029 · Cited by 80 · see citing works
Neuroinflammation in Alzheimer’s disease: Pleiotropic roles for cytokines and neuronal pentraxins
Swanson, A., Wolf, T., Sitzmann, A., & Willette, A. A. (2018) · Behavioural Brain Research · doi:10.1016/j.bbr.2018.02.015 · Cited by 72 · see citing works
Begcevic, I., Tsolaki, M., Brinc, D., Brown, M., Martinez-Morillo, E., Lazarou, I., Kozori, M., Tagaraki, F., Nenopoulou, S., Gkioka, M., et al. (2018) · F1000Research · doi:10.12688/f1000research.15095.1 · Cited by 51 · see citing works
Magdalinou, N. K., Noyce, A. J., Pinto, R., Lindstrom, E., Holmén-Larsson, J., Holtta, M., Blennow, K., Morris, H. R., Skillbäck, T., Warner, T. T., et al. (2017) · Parkinsonism & Related Disorders · doi:10.1016/j.parkreldis.2017.01.016 · Cited by 49 · see citing works
Lim, B., Tsolaki, M., Soosaipillai, A., Brown, M., Zilakaki, M., Tagaraki, F., Fotiou, D., Koutsouraki, E., Grosi, E., Prassas, I., et al. (2019) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm-2019-0428 · Cited by 25 · see citing works
- Authors
- Yin, G. N., Lee, H. W., Cho, J.-Y., & Suk, K.
- Affiliations
- Kyungpook National University (KR)
- Corresponding author
- Suk, Kyoungho
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 2009-04
- Volume
- 1265
- Pages
- 158-170
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W2058471763
- Cited by
- 143
- References
- 49
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 7 · 2023: 4 · 2022: 8 · 2021: 6 · 2020: 20 · 2019: 8 · 2018: 14 · 2017: 8 · 2016: 8 · 2015: 7 · 2014: 9 · 2013: 7 · 2012: 10
- FWCI
- 6.85
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Foundation of Korea (KRF-2006-005-J04202)
- Korea Science and Engineering Foundation
- Ministry of Education, Science and Technology (2008-04090)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Immunology and Microbiology › Immunology › Biomarkers in Disease Mechanisms
- Keywords
- cerebrospinal fluid biomarkers, neurodegenerative diseases, Alzheimer's disease, Parkinson's disease, alpha-dystroglycan, contactin-1, CNTN2, CNDP1, SPARCL1, serum biomarkers
- MeSH terms
- Pentraxins, Aged, Alzheimer Disease, C-Reactive Protein, Calcium-Binding Proteins, Chromatography, Liquid, Cognition Disorders, Dipeptidases, Humans, Middle Aged, Nerve Tissue Proteins, Parkinson Disease, Blotting, Western, Electrophoresis, Gel, Two-Dimensional, Biomarkers, Cell Adhesion Molecules, Neuronal, Extracellular Matrix Proteins, Neural Cell Adhesion Molecules, Protein Isoforms, Cell Line, Tumor, Dystroglycans, Tandem Mass Spectrometry, Contactins, Contactin 1, Contactin 2
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
