The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Estradiol or genistein prevent Alzheimer's disease-associated inflammation correlating with an increase PPARγ expression in cultured astrocytes
Abstract
Inflammation has been implicated in neurodegenerative disorders such as Alzheimer's disease (AD). The main inflammatory players in AD are the glial cells which initiate the inflammatory response. One of the earliest neuropathological changes in AD is the accumulation of astrocytes at sites of A beta deposition. It is desirable to find methods of tipping the balance towards anti-inflammatory state. Estrogenic compounds have shown anti-inflammatory and also antioxidant activity. Astrocytes were pretreated with 17-beta estradiol or with genistein, and 48 h later treated with 5 microM amyloid beta (A beta) for 24 h. We found that A beta induces inflammatory mediators, such as cyclooxygenase 2 (COX-2), inducible nitric oxide synthase (iNOS), interleukin 1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF-alpha). All these effects were prevented when cells were pretreated with estradiol or genistein, demonstrating anti-inflammatory effects of estradiol or genistein in astrocytes in primary culture. The A beta-stimulated expression of pro-inflammatory genes in cells is antagonized by the action of the PPARs (peroxisome proliferator activated receptors). Here we detected an increase in PPAR-gamma expression in astrocytes in primary culture treated with A beta and estradiol or soy isoflavone genistein. Thus, some of the anti-inflammatory effects of estrogenic compounds may be mediated and activated by PPARs suppressing a diverse array of inflammatory responses caused by A beta in astrocytes in primary culture.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“the increased levels of IL-1β and TNF-α induced by Aβ were modulated treating astrocytes with genistein (Valles et al., 2010)”
From Exploring the Therapeutic Potential of Phytochemicals in Alzheimer’s Disease: Focus on Polyphenols and Monoterpenes (2022) · cites this paper as Valles et al., 2010
Citations
Cites
Writing Group for the Women's Health Initiative Investigators (2002) · JAMA: The Journal of the American Medical Association · doi:10.1001/jama.288.3.321 · Cited by 15,889
A simplification of the protein assay method of Lowry et al. which is more generally applicable
Peterson, G. L. (1977) · Analytical Biochemistry · doi:10.1016/0003-2697(77)90043-4 · Cited by 8,531
Alzheimer's Disease: Genes, Proteins, and Therapy
Selkoe, D. J. (2001) · Physiological Reviews · doi:10.1152/physrev.2001.81.2.741 · Cited by 6,063
Wyss-Coray (2006) · doi:10.1038/nm1484 · Cited by 1,284
Widespread Peroxynitrite-Mediated Damage in Alzheimer’s Disease
Smith, M. A., Richey Harris, P. L., Sayre, L. M., Beckman, J. S., & Perry, G. (1997) · The Journal of Neuroscience · doi:10.1523/jneurosci.17-08-02653.1997 · Cited by 1,239
Neurotoxicity of a Fragment of the Amyloid Precursor Associated with Alzheimer's Disease
Yankner, B. A., Dawes, L. R., Fisher, S., Villa-Komaroff, L., Oster-Granite, M. L., & Neve, R. L. (1989) · Science · doi:10.1126/science.2474201 · Cited by 907
Rocca, W. A., Bower, J. H., Maraganore, D. M., Ahlskog, J. E., Grossardt, B. R., de Andrade, M., & Melton, L. J. (2007) · Neurology · doi:10.1212/01.wnl.0000276984.19542.e6 · Cited by 827
Borrás, C., Sastre, J., García-Sala, D., Lloret, A., Pallardó, F. V., & Viña, J. (2003) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(02)01356-4 · Cited by 654
Inflammation and the Degenerative Diseases of Aging
McGeer, P. L., & McGeer, E. G. (2004) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1332.007 · Cited by 530
Alzheimer Disease and Oxidative Stress
Perry, G., Cash, A. D., & Smith, M. A. (2002) · BioMed Research International · doi:10.1155/s1110724302203010 · Cited by 455
Comalada, M., Ballester, I., Bailón, E., Sierra, S., Xaus, J., Gálvez, J., Medina, F. S. D., & Zarzuelo, A. (2006) · Biochemical Pharmacology · doi:10.1016/j.bcp.2006.07.016 · Cited by 440
Contribution of inflammatory processes to Alzheimer's disease: molecular mechanisms
Sastre, M., Klockgether, T., & Heneka, M. T. (2006) · International Journal of Developmental Neuroscience · doi:10.1016/j.ijdevneu.2005.11.014 · Cited by 426
Estrogen receptor-α mediates the brain antiinflammatory activity of estradiol
Vegeto, E., Belcredito, S., Etteri, S., Ghisletti, S., Brusadelli, A., Meda, C., Krust, A., Dupont, S., Ciana, P., Chambon, P., et al. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1531957100 · Cited by 384
Sastre, M., Dewachter, I., Rossner, S., Bogdanovic, N., Rosen, E., Borghgraef, P., Evert, B. O., Dumitrescu-Ozimek, L., Thal, D. R., Landreth, G., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0503839103 · Cited by 373
Vegeto, E., Benedusi, V., & Maggi, A. C. (2008) · Frontiers in Neuroendocrinology · doi:10.1016/j.yfrne.2008.04.001 · Cited by 364
Causes of oxidative stress in Alzheimer disease
Zhu, X., Su, B., Wang, X., Smith, M. A., & Perry, G. (2007) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-007-7218-4 · Cited by 336
Inflammaging as a prodrome to Alzheimer's disease
Giunta, B., Fernandez, F., Nikolic, W. V., Obregon, D., Rrapo, E., Town, T., & Tan, J. (2008) · Journal of Neuroinflammation · doi:10.1186/1742-2094-5-51 · Cited by 327
Dang, Z.-C., Audinot, V., Papapoulos, S. E., Boutin, J. A., & Löwik, C. W. G. M. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m209483200 · Cited by 323
HENEKA, M., & LANDRETH, G. (2007) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2007.04.016 · Cited by 317
Effects of Combination Estrogen Plus Progestin Hormone Treatment on Cognition and Affect
Resnick, S. M., Maki, P. M., Rapp, S. R., Espeland, M. A., Brunner, R. L., Coker, L. H., Granek, I. A., Hogan, P. E., Ockene, J. K., & Shumaker, S. A. (2006) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2005-2097 · Cited by 314
Akama, K. T., & Van Eldik, L. J. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.11.7918 · Cited by 287
Vallés, S. L., Blanco, A. M., Pascual, M., & Guerri, C. (2004) · Brain Pathology · doi:10.1111/j.1750-3639.2004.tb00079.x · Cited by 271
Borrás, C., Gambini, J., Gómez-Cabrera, M. C., Sastre, J., Pallardó, F., Mann, G., & Viña, J. (2005) · Aging Cell · doi:10.1111/j.1474-9726.2005.00151.x · Cited by 257
Estrogen, cognition and female ageing
Genazzani, A. R., Pluchino, N., Luisi, S., & Luisi, M. (2007) · Human Reproduction Update · doi:10.1093/humupd/dml042 · Cited by 237
Baker, A. E., Brautigam, V. M., & Watters, J. J. (2004) · Endocrinology · doi:10.1210/en.2004-0619 · Cited by 227
Lüth, H., Münch, G. W., & Arendt, T. (2002) · Brain Research · doi:10.1016/s0006-8993(02)03280-8 · Cited by 223
Inflammation, anti-inflammatory agents and Alzheimer disease: The last 12 years
McGeer, P. L., Rogers, J., & McGeer, E. G. (2006) · Journal of Alzheimer s Disease · doi:10.3233/jad-2006-9s330 · Cited by 222
Mahn, K., Borrás, C., Knock, G. A., Taylor, P. D., Khan, I. Y., Sugden, D., Poston, L., Ward, J. P. T., Sharpe, R. M., Viña, J., et al. (2005) · The FASEB Journal · doi:10.1096/fj.05-4008fje · Cited by 195
Lüth, H.-J., Holzer, M., Gärtner, U., Staufenbiel, M., & Arendt, T. (2001) · Brain Research · doi:10.1016/s0006-8993(01)02758-5 · Cited by 183
Borrás, C., Gambini, J., Gómez‐Cabrera, M. C., Sastre, J., Pallardó, F. V., Mann, G. E., & Viña, J. (2006) · The FASEB Journal · doi:10.1096/fj.05-5522fje · Cited by 180
Neuroprotective effect of genistein against beta amyloid-induced neurotoxicity
Bang, O. Y., Hong, H., Kim, D. H., Kim, H., Boo, J. H., Huh, K., & Mook‐Jung, I. (2004) · Neurobiology of Disease · doi:10.1016/j.nbd.2003.12.017 · Cited by 123
PPARs as Drug Targets to Modulate Inflammatory Responses?
Genolet, R., Wahli, W., & Michalik, L. (2004) · Current Drug Targets - Inflammation & Allergy · doi:10.2174/1568010042634578 · Cited by 119
Murakami, A., Matsumoto, K., Koshimįzu, K., & Ohigashi, H. (2003) · Cancer Letters · doi:10.1016/s0304-3835(03)00058-2 · Cited by 114
Role of astrocytes in estrogen-mediated neuroprotection
Dhandapani, K. M., & Brann, D. W. (2006) · Experimental Gerontology · doi:10.1016/j.exger.2006.06.032 · Cited by 107
Vallés, S. L., Borrás, C., Gambini, J., Furriol, J., Ortega, Á., Sastre, J., Pallardó, F. V., & Viña, J. (2007) · Aging Cell · doi:10.1111/j.1474-9726.2007.00356.x · Cited by 81
Ma, H., Sprecher, H. W., & Kolattukudy, P. E. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.46.30131 · Cited by 72
Chong, Y. H., Sung, J. H., Shin, S. A., Chung, J., & Suh, Y. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m009466200 · Cited by 65
The inflammatory process in aging
Yu, B. P., & Chung, H. Y. (2006) · Reviews in Clinical Gerontology · doi:10.1017/s0959259807002110 · Cited by 65
Vallés, S. L., Blanco, A. M. A., Azorı́n, I., Guasch, R. M., Pascual, M., Gómez-Lechón, M. J., Renau‐Piqueras, J., & Guerri, C. (2003) · Alcoholism Clinical and Experimental Research · doi:10.1097/01.alc.0000099261.87880.21 · Cited by 64
Scacchi, R., Pinto, A., Gambina, G., Rosano, A., & Corbo, R. (2007) · Brain Research · doi:10.1016/j.brainres.2006.12.078 · Cited by 43
Lu, H., Shi, J., Zhang, D., Wang, H., Hang, C., Chen, H., & Yin, H.-X. (2009) · Journal of the Neurological Sciences · doi:10.1016/j.jns.2008.12.007 · Cited by 35
Koivisto, A. M., Helisalmi, S., Pihlajamäki, J., Hiltunen, M., Koivisto, K. J., Moilanen, L., Kuusisto, J., Helkala, E., Hänninen, T., Kervinen, K., et al. (2006) · Dementia and Geriatric Cognitive Disorders · doi:10.1159/000095857 · Cited by 32
Wan, H., Yuan, Y., Qian, A., Sun, Y., & Qiao, M.-M. (2007) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2007.10.012 · Cited by 29
Estrogenic compounds inhibit gap junctional intercellular communication in mouse Leydig TM3 cells
IWASE, Y., Fukata, H., & Mori, C. (2005) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2005.08.005 · Cited by 13
Hibasami, H., Tsukada, T., Maekawa, S., & Nakashima, K. (1986) · Cancer Letters · doi:10.1016/0304-3835(86)90127-8 · Cited by 9
Cited by
Sorted by citations
Anti-inflammatory activity of natural dietary flavonoids
Pan, M., Lai, C., & Ho, C. (2010) · Food & Function · doi:10.1039/c0fo00103a · Cited by 624
Plant-derived immunomodulators: an insight on their preclinical evaluation and clinical trials
Jantan, I., Ahmad, W., & Bukhari, S. N. A. (2015) · Frontiers in Plant Science · doi:10.3389/fpls.2015.00655 · Cited by 427
Viña, J., & Lloret, A. (2010) · Journal of Alzheimer's Disease · doi:10.3233/jad-2010-100501 · Cited by 447
Neuroinflammation: Modulation by flavonoids and mechanisms of action
Spencer, J. P. E., Vafeiadou, K., Williams, R. J., & Vauzour, D. (2012) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2011.10.016 · Cited by 315
Sánchez‐López, E., Ettcheto, M., Egea, M. A., Espina, M., Cano, A. C., Calpena, A. C., Camins, A., Carmona-Ule, N., Silva, A. M., Souto, E. B., et al. (2018) · Journal of Nanobiotechnology · doi:10.1186/s12951-018-0356-z · Cited by 300
Metadata
- Authors
- Valles, S. L., Dolz-Gaiton, P., Gambini, J., Borras, C., LLoret, A., Pallardo, F. V., & Viña, J.
- Affiliations
- Universitat de València (ES)
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 2010-02
- Volume
- 1312
- Pages
- 138-144
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W2069905837
- Cited by
- 186
- References
- 45
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 10 · 2023: 11 · 2022: 16 · 2021: 14 · 2020: 14 · 2019: 10 · 2018: 11 · 2017: 9 · 2016: 13 · 2015: 9 · 2014: 15 · 2013: 11 · 2012: 18
- FWCI
- 7.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- local and national government of Spain (GVcs2007-AP-001, BFU 2005/230, BFU 2007 6583/BFI)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Keywords
- Alzheimer's disease, astrocytes, estradiol, genistein, amyloid-beta, neuroinflammation, PPARγ, estrogenic compounds, cyclooxygenase-2, inducible nitric oxide synthase, IL-1β, TNF-α, soy isoflavones, anti-inflammatory activity
- MeSH terms
- Animals, Astrocytes, Cells, Cultured, Cerebral Cortex, Dose-Response Relationship, Drug, Drug Interactions, Enzyme-Linked Immunosorbent Assay, Estradiol, Estrogens, Gene Expression Regulation, Peptide Fragments, Tumor Necrosis Factor-alpha, Amyloid beta-Peptides, Genistein, Protein Kinase Inhibitors, PPAR gamma, Rats, Cyclooxygenase 2, Nitric Oxide Synthase Type II, Interleukin-1beta
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
