Structural and enzyme kinetic studies of retrograded starch: Inhibition of α-amylase and consequences for intestinal digestion of starch
Open accessAbstract
Retrograded starch is known to be resistant to digestion. We used enzyme kinetic experiments to examine how retrogradation of starch affects amylolysis catalysed by porcine pancreatic amylase. Parallel studies employing differential scanning calorimetry, infra red spectroscopy, X-ray diffraction and NMR spectroscopy were performed to monitor changes in supramolecular structure of gelatinised starch as it becomes retrograded. The total digestible starch and the catalytic efficiency of amylase were both decreased with increasing evidence of retrogradation. A purified sample of retrograded high amylose starch inhibited amylase directly. These new findings demonstrate that amylase binds to retrograded starch. Therefore consumption of retrograded starch may not only be beneficial to health through depletion of total digestible starch, and therefore the metabolisable energy, but may also slow the rate of intestinal digestion through direct inhibition of α-amylase. Such physiological effects have important implications for the prevention and management of type 2 diabetes and cardiovascular disease.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Type 3 resistant starch forms when gelatinized starch is cooled, promoting retrogradation and increasing crystalline, enzyme-resistant structures of amylose and amylopectin.
“Type 3 resistant starch (RS3) forms when gelatinized starch is cooled, a process that promotes retrogradation and increases crystalline, enzyme-resistant structures of amylose and amylopectin [8,9,10,11,12].”
From Does Resistant Starch Formed by Cooling Pasta Decrease the Postprandial Glycemic Response in Type 1 Diabetes? A Randomized Single-Blind Crossover Study (2026) · cites this paper as [11]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
A starch hydrolysis procedure to estimate glycemic index
Goñi, I., García‐Alonso, A., & Saura-Calixto, F. D. (1997) · Nutrition Research · doi:10.1016/s0271-5317(97)00010-9 · Cited by 2,135 · see citing works
Fundamentals of Enzyme Kinetics
Cornish-Bowden, A. J. (1979) · Elsevier eBooks · doi:10.1016/c2013-0-04130-8 · Cited by 2,079 · see citing works
Sajilata, M. G., Singhal, R. S., & Kulkarni, P. R. (2006) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/j.1541-4337.2006.tb00076.x · Cited by 1,660 · see citing works
Potential beneficial effects of butyrate in intestinal and extraintestinal diseases
Canani, R. B. (2011) · World Journal of Gastroenterology · doi:10.3748/wjg.v17.i12.1519 · Cited by 1,381 · see citing works
Organisation of the external region of the starch granule as determined by infrared spectroscopy
Sevenou, O., Hill, S. E., Farhat, I. A., & Mitchell, J. R. (2002) · International Journal of Biological Macromolecules · doi:10.1016/s0141-8130(02)00067-3 · Cited by 866 · see citing works
Glycemic index: overview of implications in health and disease,,,
Jenkins, D. J., Kendall, C. W., Augustin, L. S., Franceschi, S., Hamidi, M., Marchie, A., Jenkins, A. L., & Axelsen, M. (2002) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/76.1.266s · Cited by 827 · see citing works
Fundamentals of enzyme kinetics
(1996) · Choice Reviews Online · doi:10.5860/choice.33-4507 · Cited by 658 · see citing works
Hydrothermal Modifications of Granular Starch, with Retention of the Granular Structure: A Review
Jacobs, H., & Delcour, J. A. (1998) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf980169k · Cited by 589 · see citing works
Resistant starch—a review of the physical properties and biological impact of RS3
Haralampu, S. G. (2000) · Carbohydrate Polymers · doi:10.1016/s0144-8617(99)00147-2 · Cited by 552 · see citing works
Digestion of the polysaccharides of some cereal foods in the human small intestine
Englyst, H. N., & Cummings, J. H. (1985) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/42.5.778 · Cited by 543 · see citing works
Antoni, D., Zverlov, V. V., & Schwarz, W. H. (2007) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-007-1163-x · Cited by 539 · see citing works
Molecular organization in starches: a carbon 13 CP/MAS NMR study
Gidley, M. J., & Bociek, S. M. (1985) · Journal of the American Chemical Society · doi:10.1021/ja00310a047 · Cited by 393 · see citing works
Analysis of starch amylolysis using plots for first-order kinetics
Butterworth, P., Warren, F. J., Grassby, T., Patel, H., & Ellis, P. R. (2011) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2011.10.048 · Cited by 382 · see citing works
Self-citationGlycemic index: overview of implications in health and disease,,,
Jenkins, D. J., Kendall, C. W., Augustin, L. S., Franceschi, S., Hamidi, M., Marchie, A., Jenkins, A. L., & Axelsen, M. (2002) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/76/1.266s · Cited by 379 · see citing works
Human α‐amylase and starch digestion: An interesting marriage
Butterworth, P. J., Warren, F. J., & Ellis, P. R. (2011) · Starch - Stärke · doi:10.1002/star.201000150 · Cited by 364 · see citing works
Self-citationStarch in rubbery and glassy states by FTIR spectroscopy
Capron, I., Robert, P., Colonna, P., Brogly, M., & Planchot, V. (2006) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2006.12.015 · Cited by 358 · see citing works
Kellett, G. L., & Brot‐Laroche, E. (2005) · Diabetes · doi:10.2337/diabetes.54.10.3056 · Cited by 350 · see citing works
Inhibition of α-amylase activity by cellulose: Kinetic analysis and nutritional implications
Dhital, S., Gidley, M. J., & Warren, F. J. (2015) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2015.01.039 · Cited by 273 · see citing works
Self-citationKinetics of starch digestion in sorghum as affected by particle size
Mahasukhonthachat, K., Sopade, P. A., & Gidley, M. J. (2009) · Journal of Food Engineering · doi:10.1016/j.jfoodeng.2009.06.051 · Cited by 273 · see citing works
Brayer, G. D., Sidhu, G., Maurus, R., Rydberg, E. H., Braun, C., Wang, Y., Nguyen, N. T., Overall, C. M., & Withers, S. G. (2000) · Biochemistry · doi:10.1021/bi9921182 · Cited by 266 · see citing works
Sim, L., Carly, W., Mohan, S., Naim, H. Y., Pinto, B. M., & Rose, D. R. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.078980 · Cited by 247 · see citing works
Staling of Bread: Role of Amylose and Amylopectin and Influence of Starch‐Degrading Enzymes
Hug-Iten, S., Escher, F., & Conde‐Petit, B. (2003) · Cereal Chemistry · doi:10.1094/cchem.2003.80.6.654 · Cited by 238 · see citing works
Htoon, A., Shrestha, A. K., Flanagan, B. M., Lopez-Rubio, A., Bird, A. R., Gilbert, E. P., & Gidley, M. J. (2009) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2008.06.016 · Cited by 232 · see citing works
Self-citationNichols, B. L., Avery, S. E., Sen, P., Swallow, D. M., Hahn, D., & Sterchi, E. E. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0237170100 · Cited by 202 · see citing works
Beneficial Effect of a Low Glycaemic Index Diet in Type 2 Diabetes
Wolever, T. M. S., Jenkins, D. J. A., Vuksan, V., Jenkins, A. L., Buckley, G. C., Wong, G. S., & Josse, R. G. (1992) · Diabetic Medicine · doi:10.1111/j.1464-5491.1992.tb01816.x · Cited by 195 · see citing works
The Role of Water in the Retrogradation of Wheat Starch Gels and Bread Crumb
Zeleznak, K., & Hoseney, R. C. (1986) · Cited by 193 · see citing works
Edwards, C. H., Grundy, M. M.-L., Grassby, T., Vasilopoulou, D., Frost, G. S., Butterworth, P., Berry, S. E., Sanderson, J. D., & Ellis, P. R. (2015) · American Journal of Clinical Nutrition · doi:10.3945/ajcn.114.106203 · Cited by 181 · see citing works
Self-citationMolecular order and structure in enzyme-resistant retrograded starch
Gidley, M. J., Cooke, D., Darke, A., Hoffmann, R. A., Russell, A. L., & Greenwell, P. (1995) · Carbohydrate Polymers · doi:10.1016/0144-8617(96)81387-7 · Cited by 176 · see citing works
Liu, Q., Donner, E., Yin, Y., Huang, R., & Fan, M. Z. (2005) · Food Chemistry · doi:10.1016/j.foodchem.2005.08.008 · Cited by 168 · see citing works
Dietary fiber: Basic and clinical aspects
Dutta, S. (1987) · Gastroenterology · doi:10.1016/0016-5085(87)90168-5 · Cited by 159 · see citing works
Differential Scanning Calorimetry Studies on the Crystallinity of Ageing Wheat Starch Gels
Longton, J., & Legrys, G. A. (1981) · Starch - Stärke · doi:10.1002/star.19810331204 · Cited by 159 · see citing works
Bompard, C., Astier, J. P., Marchis-Mouren, G. J., Cambillau, C., & Payan, F. (1996) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1996.0561z.x · Cited by 157 · see citing works
Mass Transfer and Nutrient Absorption in a Simulated Model of Small Intestine
Tharakan, A., Norton, I. T., Fryer, P. J., & Bakalis, S. (2010) · Journal of Food Science · doi:10.1111/j.1750-3841.2010.01659.x · Cited by 150 · see citing works
Warren, F. J., Royall, P. G., Gaisford, S., Butterworth, P., & Ellis, P. R. (2011) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2011.05.062 · Cited by 142 · see citing works
Self-citationAn investigation of the action of porcine pancreatic α-amylase on native and gelatinised starches
Slaughter, S. L., Ellis, P. R., & Butterworth, P. (2001) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/s0304-4165(00)00162-8 · Cited by 139 · see citing works
Self-citationA simplified colorimetric procedure for determination of amylose in maize starches
Knutson, C. A. (1986) · Cereal Chemistry · Cited by 135 · see citing works
Slaughter, S. L., Ellis, P. R., Jackson, E. C., & Butterworth, P. (2002) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/s0304-4165(02)00209-x · Cited by 123 · see citing works
Self-citationMachius, M., Vértesy, L., Huber, R., & Wiegand, G. (1996) · Journal of Molecular Biology · doi:10.1006/jmbi.1996.0410 · Cited by 120 · see citing works
Park, E. Y., Baik, B., & Lim, S.-T. (2009) · Journal of Cereal Science · doi:10.1016/j.jcs.2009.02.004 · Cited by 115 · see citing works
Structural studies of starches with different water contents
Bogracheva, T. Y., Wang, Y., Wang, T. L., & Hedley, C. L. (2002) · Biopolymers · doi:10.1002/bip.10190 · Cited by 109 · see citing works
Fermentation and the Production of Short-Chain Fatty Acids in the Human Large Intestine
Cummings, J. H., & Branch, W. J. (1986) · Springer eBooks · doi:10.1007/978-1-4613-2111-8_10 · Cited by 98 · see citing works
Qian, M., Haser, R., & Payan, F. (1995) · Protein Science · doi:10.1002/pro.5560040414 · Cited by 95 · see citing works
Roberts, P. J. P., & Whelan, W. J. (1960) · Biochemical Journal · doi:10.1042/bj0760246 · Cited by 89 · see citing works
Evaluation of variations in amylose–iodine absorbance spectra
Knutson, C. A. (2000) · Carbohydrate Polymers · doi:10.1016/s0144-8617(99)00126-5 · Cited by 87 · see citing works
Tahir, R., Ellis, P. R., & Butterworth, P. (2010) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2010.01.055 · Cited by 81 · see citing works
Self-citationPatel, H., Day, R., Butterworth, P., & Ellis, P. R. (2014) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2014.06.089 · Cited by 77 · see citing works
Self-citationFlanagan, B. M., Gidley, M. J., & Warren, F. J. (2015) · Chemical Communications · doi:10.1039/c5cc06144j · Cited by 64 · see citing works
Self-citationStudies on Alkaline Phosphatase
Byers, D. A., Fernley, H. N., & Walker, P. G. (1972) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1972.tb01975.x · Cited by 62 · see citing works
Investigating the Mechanisms of Amylolysis of Starch Granules by Solution-State NMR
Baldwin, A. J., Egan, D. L., Warren, F. J., Barker, P. D., Dobson, C. M., Butterworth, P., & Ellis, P. R. (2015) · Biomacromolecules · doi:10.1021/acs.biomac.5b00190 · Cited by 55 · see citing works
Self-citationWarren, F. J., Perston, B. B., Royall, P. G., Butterworth, P., & Ellis, P. R. (2013) · Analytical Chemistry · doi:10.1021/ac303552s · Cited by 49 · see citing works
Self-citationDaramola, D., & Osanyinlusi (2006) · AFRICAN JOURNAL OF BIOTECHNOLOGY · doi:10.5897/ajb05.281 · Cited by 49 · see citing works
Roder, N., Gerard, C., Verel, A., Bogracheva, T. Y., Hedley, C. L., Ellis, P. R., & Butterworth, P. J. (2009) · Food Chemistry · doi:10.1016/j.foodchem.2008.07.106 · Cited by 46 · see citing works
Self-citationWarren, F. J., Butterworth, P., & Ellis, P. R. (2013) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2012.12.028 · Cited by 34 · see citing works
Self-citationCatalytic and ligand-binding properties of rat intestinal alkaline phosphatase
Malik, N., & Butterworth, P. (1977) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(77)90093-5 · Cited by 6 · see citing works
Self-citation
Flagged first, then by citations
5 of the 59 references couldn’t be matched to a known paper.
Research works printed but not linked: 4
- Byers D.A. Fernley H.N. Walker P.G. Inhibition of human-placental phosphoryl phosphatase by l-phenylalanine European Journal of Biochemistry 29 1972 197 204 5081613
- Daramola B. Osanyinlusi S.A. Investigation on modification of cassava starch using active components of ginger roots African Journal of Biotechnology 5 2006 917 921
- Kellet G. Brot-Laroche E. Apical GLUT 2 a major pathway of intestinal sugar absorption Diabetes 54 2005 3056 3062 16186415
- Roberts P.J.P. Whelan W.J. The mechanism of carbohydrase action Biochemical Journal 76 1960 246 253 14437832 PMC1204700
Cited by
Sorted by citations
Dietary polyphenols modulate starch digestion and glycaemic level: a review
Sun, L., & Miao, M. (2019) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2018.1544883 · Cited by 435 · see citing works
Gong, B., Cheng, L., Gilbert, R. G., & Li, C. (2019) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2019.06.003 · Cited by 270 · see citing works
Proença, C., Ribeiro, D., Freitas, M., & Fernandes, E. G. R. (2021) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2020.1862755 · Cited by 245 · see citing works
Martínez, M. M., Li, C., Okoniewska, M., Mukherjee, I., Vellucci, D., & Hamaker, B. R. (2018) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2018.06.021 · Cited by 231 · see citing works
Parameterizing starch chain-length distributions for structure-property relations
Li, C., Wu, A. C., Yu, W., Hu, Y., Li, E., Zhang, C., & Liu, Q. (2020) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2020.116390 · Cited by 200 · see citing works
Kashtoh, H., & Baek, K.-H. (2023) · Plants · doi:10.3390/plants12162944 · Cited by 213 · see citing works
Proença, C., Freitas, M., Ribeiro, D., Tomé, S. M., Oliveira, E. F., Viegas, M. F., Araújo, A. N., Ramos, M. J., Silva, A. M. S., Fernandes, P. A., et al. (2019) · Journal of Enzyme Inhibition and Medicinal Chemistry · doi:10.1080/14756366.2018.1558221 · Cited by 159 · see citing works
Li, C., & Hu, Y. (2020) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2020.106071 · Cited by 131 · see citing works
Mazhar, M., Zhu, Y., & Qin, L. (2023) · Foods · doi:10.3390/foods12051023 · Cited by 119 · see citing works
Effects of Polyphenols on Insulin Resistance
Williamson, G., & Sheedy, K. (2020) · Nutrients · doi:10.3390/nu12103135 · Cited by 118 · see citing works
Luo, Y. F., Xiao, Y., Shen, M., Wen, H., Ren, Y., Yang, J., Han, X., & Xie, J. (2019) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2019.105538 · Cited by 104 · see citing works
Rafiqah, S. A., Abdan, K., Saffian, H. A., Zaman, K., Jawaid, M., & Lee, C. H. (2018) · Polymers · doi:10.3390/polym10111187 · Cited by 84 · see citing works
Modification of retrogradation property of rice starch by improved extrusion cooking technology
Liu, Y., Chen, J., Wu, J., Luo, S., Chen, R., Liu, C., & Gilbert, R. G. (2019) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2019.02.089 · Cited by 82 · see citing works
Structural Basis of Resistant Starch (RS) in Bread: Natural and Commercial Alternatives
Román, L., & Martínez, M. M. (2019) · Foods · doi:10.3390/foods8070267 · Cited by 81 · see citing works
Dietary Fiber, Gastric Emptying, and Carbohydrate Digestion: A Mini‐Review
Qi, X., Al‐Ghazzewi, F. H., & Tester, R. F. (2018) · Starch - Stärke · doi:10.1002/star.201700346 · Cited by 79 · see citing works
Kadyan, S., Sharma, A., Arjmandi, B. H., Singh, P. R., & Nagpal, R. (2022) · Nutrients · doi:10.3390/nu14091726 · Cited by 78 · see citing works
In vitro inhibition of pancreatic α-amylase by spherical and polygonal starch nanoparticles
Jiang, S., Li, M., Chang, R., Xiong, L., & Sun, Q. (2017) · Food & Function · doi:10.1039/c7fo01381g · Cited by 78 · see citing works
Effect of amino acids composing rice protein on rice starch digestibility
Lu, X., Chang, R., Lu, H., Ma, R., Qiu, L., & Tian, Y. (2021) · LWT · doi:10.1016/j.lwt.2021.111417 · Cited by 77 · see citing works
Butterworth, P., Bajka, B. H., Edwards, C. H., Warren, F. J., & Ellis, P. R. (2021) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2021.11.015 · Cited by 76 · see citing works
Self-citationRomán, L., Gómez, M., Hamaker, B. R., & Martínez, M. M. (2018) · Food Chemistry · doi:10.1016/j.foodchem.2018.09.023 · Cited by 74 · see citing works
Main controllers for improving the resistant starch content in cooked white rice
Yi, X., & Li, C. (2021) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2021.107083 · Cited by 65 · see citing works
Starch digestion in the upper gastrointestinal tract of humans
Brownlee, I. A., Gill, S., Wilcox, M. D., Pearson, J. P., & Chater, P. I. (2017) · Starch - Stärke · doi:10.1002/star.201700111 · Cited by 65 · see citing works
Li, X., Fei, T., Wang, Y., Zhao, Y., Pan, Y., & Li, D. (2018) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.8b00550 · Cited by 64 · see citing works
Mu, M., Karthik, P., Chen, J., Holmes, M. J., & Ettelaie, R. (2020) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2020.106363 · Cited by 61 · see citing works
Korompokis, K., Verbeke, K., & Delcour, J. A. (2021) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12847 · Cited by 61 · see citing works
Yang, J., Hua, S., Huang, Z., Gu, Z., Cheng, L., & Hong, Y. (2021) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2021.118475 · Cited by 59 · see citing works
Poly(lactic acid) biocomposites with mango waste and organo‐montmorillonite for packaging
Lima, E. M. B., Lima, A. M., Minguita, A. P. D. S., dos Santos, N. R. R., Pereira, I. C. S., Neves, T. T. M., Gonçalves, L. F. D. C., Moreira, A. P., Middea, A., Neumann, R., et al. (2019) · Journal of Applied Polymer Science · doi:10.1002/app.47512 · Cited by 58 · see citing works
Visvanathan, R., Qader, M., Jayathilake, C., Jayawardana, B. C., Liyanage, R., & Sivakanesan, R. (2020) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.10315 · Cited by 58 · see citing works
The Effects of Starch Molecular Fine Structure on Thermal and Digestion Properties of Rice Starch
Li, C., Yu, W., & Gilbert, R. G. (2022) · Foods · doi:10.3390/foods11244012 · Cited by 58 · see citing works
Korompokis, K., Brier, N. D., & Delcour, J. A. (2019) · Food & Function · doi:10.1039/c9fo00947g · Cited by 58 · see citing works
Li, C. (2024) · Food Reviews International · doi:10.1080/87559129.2024.2347467 · Cited by 56 · see citing works
Tian, Y., Wang, Y., Zhong, Y., Møller, M. S., Westh, P., Svensson, B., & Blennow, A. (2023) · Molecules · doi:10.3390/molecules28093799 · Cited by 54 · see citing works
New definition of resistant starch types from the gut microbiota perspectives – a review
Li, C., & Hu, Y. (2022) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2022.2031101 · Cited by 53 · see citing works
Li, C., & Hu, Y. (2021) · LWT · doi:10.1016/j.lwt.2021.111942 · Cited by 50 · see citing works
Structure‐Functions Relationship of Modified Starches for Pharmaceutical and Biomedical Applications
Labelle, M., Ispas‐Szabo, P., & Mateescu, M. A. (2020) · Starch - Stärke · doi:10.1002/star.202000002 · Cited by 47 · see citing works
Li, C., & Hamaker, B. R. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.03.195 · Cited by 47 · see citing works
Li, C., & Hu, Y. (2021) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2021.1976722 · Cited by 45 · see citing works
de Chenevarin, G. D. D. L. V., Butterworth, P., Ellis, P. R., Hill, S. E., Warren, F. J., & Edwards, C. H. (2020) · Food Chemistry X · doi:10.1016/j.fochx.2020.100078 · Cited by 45 · see citing works
Self-citationRuan, Y., Zhang, R., & Xu, Y. (2022) · Food Chemistry · doi:10.1016/j.foodchem.2022.132222 · Cited by 44 · see citing works
Dual α-amylase and α-glucosidase inhibitors: recent progress from natural and synthetic resources
Jha, R., Goyal, K., Mehan, S., & Singh, G. (2025) · Bioorganic Chemistry · doi:10.1016/j.bioorg.2025.108762 · Cited by 43 · see citing works
Influence of molecular structure on the susceptibility of starch to α-amylase
Villas‐Boas, F., Yamauti, Y., Moretti, M. M. D. S., & Franco, C. M. L. (2019) · Carbohydrate Research · doi:10.1016/j.carres.2019.05.001 · Cited by 41 · see citing works
Kuruburu, M. G., Bovilla, V. R., Naaz, R., Leihang, Z., & Madhunapantula, S. V. (2022) · Phytomedicine Plus · doi:10.1016/j.phyplu.2022.100276 · Cited by 40 · see citing works
Li, E., Lv, J., Huo, D., Jia, B., & Li, C. (2023) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2023.120648 · Cited by 39 · see citing works
Li, E., Yang, X., & Li, C. (2022) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2022.01.092 · Cited by 39 · see citing works
Kable, M. E., Chin, E. L., Storms, D. H., Lemay, D. G., & Stephensen, C. B. (2021) · Journal of Nutrition · doi:10.1093/jn/nxab430 · Cited by 38 · see citing works
Retrogradation properties and in vitro digestibility of wild starch from Castanopsis sclerophylla
Ding, Y., Wan, J., Liu, C., Shi, X., Xia, X., Prakash, S., & Zhang, X. (2020) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2020.105693 · Cited by 37 · see citing works
Korompokis, K., Deleu, L. J., Brier, N. D., & Delcour, J. A. (2021) · Food Chemistry · doi:10.1016/j.foodchem.2021.130203 · Cited by 37 · see citing works
Costa, E. S., França, C. N., Fonseca, F. A. H., Kato, J. T., Bianco, H. T., de Freitas, T. T., Fonseca, H. A. R., Neto, A. M. F., & Izar, M. C. D. O. (2019) · British Journal Of Nutrition · doi:10.1017/s0007114519000576 · Cited by 36 · see citing works
Yee, J., Román, L., Pico, J., Aguirre‐Cruz, A., Bello‐Pérez, L. A., Bertoft, E., & Martínez, M. M. (2020) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2020.106351 · Cited by 35 · see citing works
Starch retrogradation in potato cells: Structure and in vitro digestion paradigm
Shu, L., Dhital, S., Junejo, S. A., Ding, L., Huang, Q., Fu, X., He, X., & Zhang, B. (2022) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2022.119261 · Cited by 36 · see citing works
Ktari, N., Salem, R. B. S.-B., Bkhairia, I., Slima, S. B., Nasri, R., Salah, R. B., & Nasri, M. (2020) · Food Bioscience · doi:10.1016/j.fbio.2020.100539 · Cited by 34 · see citing works
Ruan, Y., Xu, Y., Zhang, W., & Zhang, R. (2020) · Food Chemistry · doi:10.1016/j.foodchem.2020.128599 · Cited by 33 · see citing works
Li, C., & Hu, Y. (2021) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2021.107203 · Cited by 33 · see citing works
Thakur, M., Rai, A. K., Mishra, B. B., & Singh, S. P. (2021) · Environmental Technology & Innovation · doi:10.1016/j.eti.2021.101827 · Cited by 32 · see citing works
Duijsens, D., Gwala, S., Pallares, A. P., Pälchen, K., Hendrickx, M., & Grauwet, T. (2021) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12826 · Cited by 35 · see citing works
Li, C. (2022) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2022.120217 · Cited by 32 · see citing works
Qi, K., Cao, S., & Li, C. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.131907 · Cited by 31 · see citing works
Nurdjanah, S., Nurdin, S. U., Astuti, S., & Manik, V. (2022) · International Journal of Food Science · doi:10.1155/2022/7708172 · Cited by 31 · see citing works
A mechanistic study of chestnut starch retrogradation and its effects on in vitro starch digestion
Liu, C., Liu, S., Li, R., Zhang, X., & Chang, X. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.133803 · Cited by 30 · see citing works
Edwards, C. H., Veerabahu, A. S., Mason, A. J., Butterworth, P., & Ellis, P. R. (2021) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2021.117738 · Cited by 30 · see citing works
Self-citationUnderstanding the interplay between dietary fiber, polyphenols, and digestive enzymes
Sharma, V., Devkota, L., Kishore, N., & Dhital, S. (2025) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2025.111310 · Cited by 29 · see citing works
Low glycaemic index foods from wild barley and amylose-only barley lines
Sagnelli, D., Chessa, S., Mandalari, G., Martino, M. D., Sorndech, W., Mamone, G., Vincze, É., Buillon, G., Nielsen, D. S., Wiese, M., et al. (2017) · Journal of Functional Foods · doi:10.1016/j.jff.2017.11.028 · Cited by 29 · see citing works
Optimization of enzyme-assisted preparation and characterization of Arenga pinnata resistant starch
Zhang, L., Mei, J., Ren, M., & Fu, Z. (2020) · Food Structure · doi:10.1016/j.foostr.2020.100149 · Cited by 29 · see citing works
Strozyk, S., Rogowicz-Frontczak, A., Pilacinski, S., LeThanh-Blicharz, J., Koperska, A., & Zozulinska-Ziolkiewicz, D. (2022) · Nutrition & Diabetes · doi:10.1038/s41387-022-00196-1 · Cited by 28 · see citing works
“Starch granules are disrupted by heating in water in a process commonly known as gelatinization, this makes the molecules fully accessible to digestive enzymes [8].”
BackgroundZeng, X., Jiang, W., Li, H., Li, Q., Kokini, J. L., Du, Z., Xi, Y., & Li, J. (2023) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2023.108556 · Cited by 28 · see citing works
Li, W., Cao, R., Cao, J., Zhang, J., Yang, X., Liu, X., & Sun, L. (2024) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2024.110674 · Cited by 27 · see citing works
Sun, J., Wang, Y., Zhang, X., Rasmussen, S. K., Xiaotong, J., Song, W., Wu, D., & Shu, X. (2017) · International Journal of Food Science & Technology · doi:10.1111/ijfs.13686 · Cited by 25 · see citing works
Tian, Y., Li, M., Liu, X., Jane, J., Guo, B., & Dhital, S. (2020) · Food Chemistry · doi:10.1016/j.foodchem.2020.128702 · Cited by 25 · see citing works
The properties and preparation of functional starch: a review
Fan, L., Ye, Q., Lü, W., Chen, D., Zhang, C., LiHan, X., Meng, X., Lee, Y., Wang, H., & Xiao, C. (2021) · Food Reviews International · doi:10.1080/87559129.2021.2015375 · Cited by 24 · see citing works
Zeng, X., Kang, H., Chen, L., Shen, X., & Zheng, B. (2023) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2023.121535 · Cited by 23 · see citing works
Paternina‐Contreras, A. L., Andrade-Pizarro, R. D., & Figueroa-Flórez, J. A. (2025) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.70184 · Cited by 23 · see citing works
Thakur, Y., Thory, R., Sandhu, K. S., Kaur, M., Sinhmar, A., & Pathera, A. K. (2021) · Journal of Food Science and Technology · doi:10.1007/s13197-021-04974-0 · Cited by 23 · see citing works
Sun, X., Meng, L., & Tang, X. (2021) · Journal of Cereal Science · doi:10.1016/j.jcs.2021.103234 · Cited by 22 · see citing works
Wang, P., Li, D., Hou, C., Tao, Y., Yang, R., Gu, Z., & Jiang, D. (2022) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.1c07722 · Cited by 22 · see citing works
Effects of ultrasound combined with hydrothermal treatment on the quality of brown rice
Liu, X., Guo, Y., Wang, S., Wang, X., Wang, Z., & Wang, Z. (2024) · LWT · doi:10.1016/j.lwt.2024.115874 · Cited by 22 · see citing works
Mulargia, L. I., Lemmens, E., Reyniers, S., Gebruers, K., Wouters, A. G. B., Warren, F. J., Goderis, B., & Delcour, J. A. (2024) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2024.122440 · Cited by 22 · see citing works
Self-citationDuan, S., Zhang, X., & Li, C. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.141728 · Cited by 21 · see citing works
Corrado, M., Ahn‐Jarvis, J. H., Fahy, B. F., Savva, G. M., Edwards, C. H., & Hazard, B. A. (2022) · Food & Function · doi:10.1039/d1fo03085j · Cited by 21 · see citing works
Self-citationZhang, Y., Xing, B., Kong, D., Gu, Z., Yu, Y., Zhang, Y., & Li, D. (2023) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.127895 · Cited by 20 · see citing works
Mokhtari, Z., Jafari, S. M., Ziaiifar, A. M., & Cacciotti, I. (2023) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.128237 · Cited by 19 · see citing works
Corrado, M., Cherta-Murillo, A., Chambers, E. S., Wood, A. J., Plummer, A., Lovegrove, A., Edwards, C. H., Frost, G. S., & Hazard, B. A. (2019) · Food & Function · doi:10.1039/c9fo02460c · Cited by 19 · see citing works
Self-citationLi, W., Song, Y. P., Sun, W., Yang, X., Liu, X., & Sun, L. (2021) · Frontiers in Nutrition · doi:10.3389/fnut.2021.766756 · Cited by 18 · see citing works
Fong, J. N., Miketinas, D. C., Moore, L. W., Nguyen, D. T., Graviss, E. A., Ajami, N. J., & Patterson, M. A. (2022) · Nutrients · doi:10.3390/nu14020268 · Cited by 18 · see citing works
Yi, X., Shao, S., Zhang, X., Yu, W., Zhang, B., Liu, H., Gilbert, R. G., & Li, C. (2023) · Food & Function · doi:10.1039/d2fo03737h · Cited by 17 · see citing works
Inhibitory Mechanism of Quercimeritrin as a Novel α-Glucosidase Selective Inhibitor
Guo, F., An, J., Wang, M., Zhang, W., Chen, C., Mao, X., Liu, S., Wang, P., & Ren, F. (2023) · Foods · doi:10.3390/foods12183415 · Cited by 17 · see citing works
Influence of Storage Temperature on Starch Retrogradation and Digestion of Chinese Steamed Bread
Li, C., Shao, S., Yi, X., Cao, S., Yu, W., Zhang, B., Liu, H., & Gilbert, R. G. (2024) · Foods · doi:10.3390/foods13040517 · Cited by 17 · see citing works
Song, J., Zong, J., Ma, C., Chen, S., Li, H., & Zhang, D. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.06.154 · Cited by 17 · see citing works
Ojah, E. O., Moronkola, D. O., & Adeniyi-Akee, M. A. (2020) · Iberoamerican Journal of Medicine · doi:10.53986/ibjm.2020.0046 · Cited by 16 · see citing works
Yu, X., Zhang, K., Zhu, X., Lv, H., & Wu, J. (2023) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.127372 · Cited by 16 · see citing works
Enzyme applications in baking: From dough development to shelf-life extension
Chowdhury, M. A. H., Sarkar, F., Reem, C. S. A., Rahman, S. M., Mahamud, A. G. M. S. U., Rahman, M. A., & Ashrafudoulla, M. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.137020 · Cited by 19 · see citing works
The Physicochemical and Rheological Properties of Green Banana Flour–Wheat Flour Bread Substitutions
Bashmil, Y. M., Békés, F., Ruderman, M., Suleria, H. A. R., Appels, R., & Dunshea, F. R. (2025) · Plants · doi:10.3390/plants14020207 · Cited by 15 · see citing works
Sillu, D., Agnihotri, S., & Reddy, S. M. (2022) · ACS Sustainable Chemistry & Engineering · doi:10.1021/acssuschemeng.2c01547 · Cited by 15 · see citing works
Fighting Type-2 Diabetes: Present and Future Perspectives
Yu, C., Fu, Y., Fang, Y., Zhang, N., Sun, R., Zhao, D., Feng, Y., & Zhang, B. (2017) · Current Medicinal Chemistry · doi:10.2174/0929867324666171009115356 · Cited by 16 · see citing works
Endogenous rice endosperm hemicellulose slows in vitro starch digestibility
Sun, J., Fu, J., Wang, Y., Ye, H., Wu, D., & Shu, X. (2018) · International Journal of Food Science & Technology · doi:10.1111/ijfs.13988 · Cited by 15 · see citing works
Vaitkevičienė, R., Bendoraitienė, J., Degutytė, R., Švažas, M., & Žadeikė, D. (2022) · Polymers · doi:10.3390/polym14173662 · Cited by 14 · see citing works
Li, C., & Li, S. (2022) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.12311 · Cited by 14 · see citing works
Shen, X., Ma, C., Wang, Y., Liu, X., Wang, B., Fu, Y., Zhang, G., Yang, Y., & Zhang, N. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.145452 · Cited by 14 · see citing works
Beh, C. Y., Cheng, E. M., Nasir, N. F. M., Kheng, E. S., Majid, M. S. A., Ridzuan, M. J. M., Khor, S. F., & Khalid, N. S. (2020) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.11.034 · Cited by 14 · see citing works
Use of Amylomaltase to Steer the Functional and Nutritional Properties of Wheat Starch
Korompokis, K., Deleu, L. J., Brier, N. D., & Delcour, J. A. (2021) · Foods · doi:10.3390/foods10020303 · Cited by 14 · see citing works
Hu, Y., Li, C., & Hou, Y. (2021) · Food & Function · doi:10.1039/d1fo02416g · Cited by 14 · see citing works
- Authors
- Patel, H., Royall, P. G., Gaisford, S., Williams, G. R., Edwards, C. H., Warren, F. J., Flanagan, B. M., Ellis, P. R., & Butterworth, P. J.
- Affiliations
- King's College London (GB)
- UCL School of Pharmacy (GB)
- The University of Queensland (AU)
- Corresponding author
- Butterworth, Peter J.
- ORCID
- Venue
- Carbohydrate Polymers
- Publisher
- Elsevier BV
- Published
- 2017-05
- Volume
- 164
- Pages
- 154-161
- ISSN
- 0144-8617 (ISSN-L)
- PMC
- PMC5374268
- OpenAlex
- W2573838612
- Cited by
- 154
- References
- 59
- Citations by year
- 2026: 14 · 2025: 18 · 2024: 18 · 2023: 16 · 2022: 19 · 2021: 26 · 2020: 19 · 2019: 12 · 2018: 6 · 2017: 6
- FWCI
- 9.78
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Carbohydrate Polymers (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Research Portal (King's College London) (submittedVersion, cc-by)
- UCL Discovery (University College London) (submittedVersion)
- Funders
- BBSRC (BB/H004866/1)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Food composition and properties
- Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Keywords
- retrograded starch, starch retrogradation, α-amylase inhibition, starch digestibility, high-amylose starch, type 2 diabetes prevention, cardiovascular disease prevention
- MeSH terms
- alpha-Amylases, Amylose, Animals, Digestion, Kinetics, Starch, Swine
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
