Cited by 476Cellular transport and secretionCaveolin-1 and cellular processesMicrotubule and mitosis dynamics

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Coupling between Clathrin-Coated-Pit Invagination, Cortactin Recruitment, and Membrane Scission Observed in Live Cells

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Merrifield, Christien J., Perrais, David, Zenisek, David·2005·Cell

DOI 10.1016/j.cell.2005.03.015

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Abstract

During clathrin-mediated endocytosis, membrane scission marks the isolation of a cargo-laden clathrin-coated pit (CCP) from the cell exterior. Here we used live-cell imaging of a pH-sensitive cargo to visualize the formation of clathrin-coated vesicles (CCVs) at single CCPs with a time resolution of seconds. We show that CCPs are highly dynamic and can produce multiple vesicles in succession. Using alternating evanescent field and epifluorescence illumination, we show that CCP invagination and scission are tightly coupled, with scission coinciding with maximal displacement of CCPs from the plasma membrane and with peak recruitment of cortactin-DsRed, a dynamin and F-actin binding protein. Finally, perturbing actin polymerization with latrunculin-B drastically reduces the efficiency of membrane scission and affects many aspects of CCP dynamics. We propose that CCP invagination, actin polymerization, and CCV formation are highly coordinated for efficient endocytosis.

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Curvature of clathrin-coated pits driven by epsin
Ford, M. G. J., Mills, I. G., Peter, B. J., Vallis, Y., Praefcke, G. J. K., Evans, P. R., & McMahon, H. T. (2002) · Nature · doi:10.1038/nature01020 · Cited by 1,019
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Clathrin
Kirchhausen, T. (2000) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.69.1.699 · Cited by 560

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Aggressiveness of HNSCC tumors depends on expression levels of cortactin, a gene in the 11q13 amplicon
Clark, E. S., Brown, B. T., Whigham, A. S., Kochaishvili, A., Yarbrough, W., & Weaver, A. M. (2008) · Oncogene · doi:10.1038/onc.2008.389 · Cited by 94
“trafficking, data from other laboratories implicate cortactin in endocytosis and trafficking of model proteins from the Golgi apparatus (Cao et al., 2003, 2005; Merrifield et al., 2005; Zhu et al., 2005).”
Synergistic BAR–NPF interactions in actin-driven membrane remodeling
Suetsugu, S., & Gautreau, A. M. (2012) · Trends in Cell Biology · doi:10.1016/j.tcb.2012.01.001 · Cited by 94
Rabenosyn-5 defines the fate of the transferrin receptor following clathrin-mediated endocytosis
Navaroli, D. M., Bellve, K. D., Standley, C., Lifshitz, L. M., Cardia, J. P., Lambright, D. G., Leonard, D. M., Fogarty, K. E., & Corvera, S. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1115495109 · Cited by 87
Distinct Roles for F-BAR Proteins Cdc15p and Bzz1p in Actin Polymerization at Sites of Endocytosis in Fission Yeast
Arasada, R., & Pollard, T. D. (2011) · Current Biology · doi:10.1016/j.cub.2011.07.046 · Cited by 82
“In all carefully studied systems actin assembly during endocytosis depends on Arp2/3 complex and its nucleation promoting factors [5, 13, 17-20].”
Actin Dynamics Regulate Multiple Endosomal Steps during Kaposi's Sarcoma-Associated Herpesvirus Entry and Trafficking in Endothelial Cells
Greene, W., & Gao, S. (2009) · PLoS Pathogens · doi:10.1371/journal.ppat.1000512 · Cited by 93
“More recent studies using total internal reflection fluorescence microscopy (TIR-FM) technology to observe individual endocytic events recorded a burst of actin polymerization with concomitant internalization of clathrin-coated pits and movement of clathrincoated vesicles into the cell interior, but did not determine…”
Src Triggers Circular Ruffling and Macropinocytosis at the Apical Surface of Polarized MDCK Cells
Mettlen, M., Platek, A., van der Smissen, P., Carpentier, S., Amyere, M., Lanzetti, L., de Diesbach, P., Tyteca, D., & Courtoy, P. (2006) · Traffic : the International Journal of Intracellular Transport · doi:10.1111/j.1600-0854.2006.00412.x · Cited by 92
Rab35 GTPase Triggers Switch-like Recruitment of the Lowe Syndrome Lipid Phosphatase OCRL on Newborn Endosomes
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“Then, we determined the exact timing of recruitment of Rab35 and OCRL with respect to CCV scission from the plasma membrane, using a state-of-the-art ‘‘pulsed pH’’ (ppH) assay established previously [39, 40].”
Self-citation
Cortactin Controls Cell Motility and Lamellipodial Dynamics by Regulating ECM Secretion
Sung, B., Zhu, X.-D., Kaverina, I., & Weaver, A. M. (2011) · Current Biology · doi:10.1016/j.cub.2011.06.065 · Cited by 91
“A number of studies have examined the role of cortactin in endocytosis, and most have found that KD or inhibition of cortactin reduces endocytic rates [29–36].”
Integrin-beta3 clusters recruit clathrin-mediated endocytic machinery in the absence of traction force
Yu, C., Rafiq, N. M., Cao, F., Zhou, Y., Krishnasamy, A., Biswas, K. H., Ravasio, A., Chen, Z., Wang, Y., Kawauchi, K., et al. (2015) · Nature Communications · doi:10.1038/ncomms9672 · Cited by 93
INSULIN CONTROLS THE SPATIAL DISTRIBUTION OF GLUT4 ON THE CELL SURFACE THROUGH REGULATION OF ITS POST-FUSION DISPERSAL
Stenkula, K. G., Lizunov, V., Cushman, S. W., & Zimmerberg, J. (2010) · Cell Metabolism · doi:10.1016/j.cmet.2010.08.005 · Cited by 90
“, 2006) and can be attributed to formation of single endocytic vesicles when correlated with the uptake of a particular cargo (Merrifield et al., 2005).”
The role of Alzheimer's disease risk genes in endolysosomal pathways
Szabo, M. P., Mishra, S., Knupp, A., & Young, J. E. (2021) · Neurobiology of Disease · doi:10.1016/j.nbd.2021.105576 · Cited by 87
“Clathrin forms a complex lattice like structure around vesicles formed by membrane invaginations(Marsh and McMahon, 1999; Merrifield et al., 2005; Pearse, 1976; Ungewickell and Hinrichsen, 2007).”
The HIV Envelope but Not VSV Glycoprotein Is Capable of Mediating HIV Latent Infection of Resting CD4 T Cells
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“The involvement of the cortical actin in early endocytosis is largely limited to membrane scission of clathrin-coated pits [57].”
Background
A motif in the clathrin heavy chain required for the Hsc70/auxilin uncoating reaction.
Rapoport, I., Boll, W., Yu, A.-A., Böcking, T., & Kirchhausen, T. (2008) · Molecular Biology of the Cell · doi:10.1091/mbc.e07-09-0870 · Cited by 85
“Removal of the coat (uncoating), essential for subsequent vesicle fusion and cargo delivery, is the last step in the clathrin cycle (Ehrlich et al., 2004; Merrifield et al., 2005; Lee et al., 2006; Massol et al., 2006).”
Nanoparticle entry into cells; the cell biology weak link
Griffiths, G. W., Grüenberg, J., Marsh, M., Wohlmann, J., Jones, A. T., & Parton, R. G. (2022) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2022.114403 · Cited by 101
Evolutionarily unique mechanistic framework of clathrin-mediated endocytosis in plants
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Background
Determinants of endocytic membrane geometry, stability, and scission
Kishimoto, T., Sun, Y., Buser, C., Liu, J., Michelot, A., & Drubin, D. G. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1113413108 · Cited by 79
Dynamics of clathrin and adaptor proteins during endocytosis
Rappoport, J. Z., Kemal, S., Benmerah, A., & Simon, S. M. (2006) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00160.2006 · Cited by 72
Fluorescence Applications in Molecular Neurobiology
Taraska, J. W., & Zagotta, W. N. (2010) · Neuron · doi:10.1016/j.neuron.2010.02.002 · Cited by 93
Plasma Membrane-Associated SCAR Complex Subunits Promote Cortical F-Actin Accumulation and Normal Growth Characteristics in Arabidopsis Roots
Dyachok, J., Shao, M., Vaughn, K. C., Bowling, A. J., Facette, M. R., Djakovic, S. N., Clark, L. G., & Smith, L. G. (2008) · Molecular Plant · doi:10.1093/mp/ssn059 · Cited by 79
“Regulation of membrane dynamics seems to be the most obvious possibility given the well established role played by the Arp2/3 complex in endocytosis in yeast and mammalian cells (Kaksonen et al., 2006; Sun et al., 2006; Merrifield et al., 2004, 2005).”
Background
Interaction between Epsin/Yap180 Adaptors and the Scaffolds Ede1/Pan1 Is Required for Endocytosis
Maldonado‐Báez, L., Dores, M. R., Perkins, E. M., Drivas, T. G., Hicke, L., & Wendland, B. (2008) · Molecular Biology of the Cell · doi:10.1091/mbc.e07-10-1019 · Cited by 67
“…trigger the transition from early (cargo-gathering) to late (vesicle scission) events in endocytic internalization, although the mechanism is unknown (Ehrlich et al., 2004; Kaksonen et al., 2005; Merrifield et al., 2005; Naslavsky and Caplan, 2005; Newpher et al., 2005; Newpher and Lemmon, 2006).”
Background
EFC/F‐BAR proteins and the N‐WASP–WIP complex induce membrane curvature‐dependent actin polymerization
Takano, K., Toyooka, K., & Suetsugu, S. (2008) · EMBO Journal · doi:10.1038/emboj.2008.216 · Cited by 75
Multiple repeat elements within the FAM21 tail link the WASH actin regulatory complex to the retromer
Jia, D., Gomez, T. S., Billadeau, D. D., & Rosen, M. K. (2012) · Molecular Biology of the Cell · doi:10.1091/mbc.e11-12-1059 · Cited by 200
“There, N-WASP controls actin dynamics, which is important for both membrane invagination and vesicle scission (Merrifield et al., 2005; Yarar et al., 2005).”
An alternative mechanism of clathrin-coated pit closure revealed by ion conductance microscopy
Shevchuk, A., Novák, P., Taylor, M. J., Diakonov, I. A., Ziyadeh-Isleem, A., Bitoun, M., Guicheney, P., Lab, M. J., Gorelik, J., Merrifield, C. J., et al. (2012) · The Journal of Cell Biology · doi:10.1083/jcb.201109130 · Cited by 86
“By labeling different molecules and following how their fluorescence changes, it has been possible to build up a picture of the time course of the major events taking place during CME (Merrifield et al., 2002, 2005; Rappoport, 2008).”
InfluentialSelf-citation
Measuring the Hierarchy of Molecular Events During Clathrin‐Mediated Endocytosis
Loerke, D., Mettlen, M., Schmid, S. L., & Danuser, G. M. (2011) · Traffic · doi:10.1111/j.1600-0854.2011.01197.x · Cited by 76
Differential Requirements for Clathrin-dependent Endocytosis at Sites of Cell–Substrate Adhesion
Batchelder, E., & Yarar, D. (2010) · Molecular Biology of the Cell · doi:10.1091/mbc.e09-12-1044 · Cited by 74
“Distinct requirements for actin assembly during clathrin-coated vesicle formation have been reported in different cell types (Schafer et al., 1998; Fujimoto et al., 2000; Blanpied et al., 2002; Moskowitz et al., 2003; Merrifield et al., 2005; Yarar et al., 2005; Boucrot et al., 2006; Lee et al., 2006), and have also b…”
BackgroundMethodInfluential
Plasma Membrane Domains Specialized for Clathrin-mediated Endocytosis in Primary Cells*
Bellve, K., Leonard, D. M., Standley, C., Lifshitz, L. M., Tuft, R., Hayakawa, A., Corvera, S., & Fogarty, K. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m511370200 · Cited by 74
Molecularly Distinct Clathrin-Coated Pits Differentially Impact EGFR Fate and Signaling
Pascolutti, R., Algisi, V., Conte, A., Raimondi, A., Pasham, M. R., Upadhyayula, S., Gaudin, R., Maritzen, T., Barbieri, E., Caldieri, G., et al. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.05.017 · Cited by 80
“This could be due to incomplete ablation of eps15/eps15L1/epsin1 or to participation of other endocytic proteins in CCP formation, as also supported by extant literature (Kaksonen and Roux, 2018; Mettlen et al., 2009; Merrifield et al., 2005).”
Retrograde Transport: Two (or More) Roads Diverged in an Endosomal Tree?
Johannes, L., & Wunder, C. (2011) · Traffic · doi:10.1111/j.1600-0854.2011.01200.x · Cited by 79
Pak1 Phosphorylation Enhances Cortactin-N-WASP Interaction in Clathrin-Caveolin-Independent Endocytosis
Grassart, A., Meas‐Yedid, V., Dufour, A., Olivo‐Marín, J., Dautry‐Varsat, A., & Sauvonnet, N. (2010) · Traffic · doi:10.1111/j.1600-0854.2010.01075.x · Cited by 82
Regarding the Amazing Choreography of Clathrin Coats
Traub, L. M. (2011) · PLoS Biology · doi:10.1371/journal.pbio.1001037 · Cited by 73
“Furthermore, time-resolved imaging reveals that the small clathrin buds (seen as #200-nm diameter diffraction-limited spots) and large clathrin patches can have different lifetimes [19,23,24].”
Dynamin-2 Function and Dysfunction Along the Secretory Pathway
González-Jamett, A. M., Momboisse, F., Haro-Acuña, V., Bevilacqua, J., Caviedes, P., & Cárdenas, A. M. (2013) · Frontiers in Endocrinology · doi:10.3389/fendo.2013.00126 · Cited by 71
“The recruitment of dynamin at endocytosis sites (39) also depends on its interaction with SH3-domain-containing proteins and phosphoinositides present at the plasma membrane via its PRD (40) and PH domain, respectively (41).”
Background
The Generation of Curved Clathrin Coats from Flat Plaques
den Otter, W. K., & Briels, W. J. (2011) · Traffic · doi:10.1111/j.1600-0854.2011.01241.x · Cited by 64
An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis
Kaur, S., Fielding, A. B., Gassner, G., Carter, N. J., & Royle, S. J. (2014) · bioRxiv · doi:10.1101/001701 · Cited by 68
“…microscopy studies clearly show that it is recruited to a subset of CME events in human cells (Collins et al., 2011; Merrifield et al., 2002; Merrifield et al., 2005; Taylor et al., 2012; Taylor et al., 2011) and that it is important for internalisation of pathogens such as Listeria…”
Background
Dynamic Interaction of Amphiphysin with N-WASP Regulates Actin Assembly*
Yamada, H., Padilla-Parra, S., Park, S.-J., Itoh, T., Chaineau, M., Monaldi, I., Cremona, O., Benfenati, F., Camilli, P. D., Coppey-Moisan, M., et al. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.064204 · Cited by 68
The F-BAR protein CIP4 promotes GLUT4 endocytosis through bidirectional interactions with N-WASp and Dynamin-2
Hartig, S. M., Ishikura, S., Hicklen, R. S., Feng, Y., Blanchard, E. G., Voelker, K., Pichot, C., Grange, R., Raphael, R., Klip, A., et al. (2009) · Journal of Cell Science · doi:10.1242/jcs.041343 · Cited by 68
“In support of our observations, recent evidence has also shown periodic activity of N-WASp and Dynamin during clathrin-mediated endocytosis (Merrifield et al., 2002; Merrifield et al., 2005).”
Background
Podocyte endocytosis in the regulation of the glomerular filtration barrier
Inoue, K., & Ishibe, S. (2015) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.00136.2015 · Cited by 65
“For example, dynamin, a GTPase that mediates the fission reaction during clathrin-mediated endocytosis, has been shown to interact with actin-nucleating protein Arp2/3 (57) and cortactin (66, 69).”
Background
Morphological changes of plasma membrane and protein assembly during clathrin-mediated endocytosis
Yoshida, A., Sakai, N., Uekusa, Y., Imaoka, Y., Itagaki, Y., Suzuki, Y., & Yoshimura, S. H. (2018) · PLoS Biology · doi:10.1371/journal.pbio.2004786 · Cited by 68
The proposed functions of membrane curvatures mediated by the BAR domain superfamily proteins
Suetsugu, S. (2010) · The Journal of Biochemistry · doi:10.1093/jb/mvq049 · Cited by 59
Immunostimulation Effect of Jellyfish Collagen
Sugahara, T., Ueno, M., Goto, Y., Shiraishi, R., Doi, M., Akiyama, K., & Yamauchi, S. (2006) · Bioscience, biotechnology and biochemistry · doi:10.1271/bbb.60076 · Cited by 64
How can we use the endocytosis pathways to design nanoparticle drug-delivery vehicles to target cancer cells over healthy cells?
Cong, V. T., Houng, J. L., Kavallaris, M., Chen, X., Tilley, R. D., & Gooding, J. J. (2022) · Chemical Society Reviews · doi:10.1039/d1cs00707f · Cited by 129
African swine fever virus-cell interactions: From virus entry to cell survival
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“These molecules take part in membrane bending and elongation, and final fission of the endocytic vesicle ( Fig. 1) (Merrifield et al., 2005; Taylor et al., 2011) .”
Background
Deletion of the COOH-terminal domain of CXC chemokine receptor 4 leads to the down-regulation of cell-to-cell contact, enhanced motility and proliferation in breast carcinoma cells.
Ueda, Y., Neel, N., Schutyser, E., Raman, D., & Richmond, A. (2006) · Cancer Research · doi:10.1158/0008-5472.can-05-3579 · Cited by 63
The initiation of clathrin-mediated endocytosis is mechanistically highly flexible
Brach, T., Godlee, C., Moeller-Hansen, I., Boeke, D., & Kaksonen, M. (2014) · Current Biology · doi:10.1016/j.cub.2014.01.048 · Cited by 62
Dynamics of Intracellular Clathrin/AP1- and Clathrin/AP3-Containing Carriers
Kural, C., Tacheva-Grigorova, S. K., Boulant, S., Cocucci, E., Baust, T., Duarte, D., & Kirchhausen, T. L. (2012) · Cell Reports · doi:10.1016/j.celrep.2012.09.025 · Cited by 68
“…coverslip have been studied by two-dimensional (2D) time-lapse imaging, using confocal or total internal reflection fluorescence microscopy (Cocucci et al., 2012; Ehrlich et al., 2004; Merrifield et al., 2005; Mettlen et al., 2009; Saffarian et al., 2009; Taylor et al., 2011; Yarar et al., 2005).”
Method
Dual pools of actin at presynaptic terminals
Bleckert, A., Photowala, H., & Alford, S. T. (2012) · Journal of Neurophysiology · doi:10.1152/jn.00789.2011 · Cited by 68
Local actin polymerization during endocytic carrier formation
Hinze, C., & Boucrot, E. (2018) · Biochemical Society Transactions · doi:10.1042/bst20170355 · Cited by 68
Actin acting at the Golgi
Egea, G., Serra‐Peinado, C., Salcedo-Sicilia, L., & Gutiérrez-Martínez, E. (2013) · Histochemistry and Cell Biology · doi:10.1007/s00418-013-1115-8 · Cited by 63
“2a) similarly to what happens during endocytosis (Merrifield 2004; Merrifield et al. 2005; Taylor et al. 2012), and/or for its translocation to closely arranged microtubule tracks”
Background
AP2 adaptor complex mediates bile salt export pump internalization and modulates its hepatocanalicular expression and transport function
Hayashi, H., Inamura, K., Aida, K., Naoi, S., Horikawa, R., Nagasaka, H., Takatani, T., Fukushima, T., Hattori, A., Yabuki, T., et al. (2012) · Hepatology · doi:10.1002/hep.25591 · Cited by 62
Quantitative analysis of endocytosis with cytoplasmic pHluorin chimeras
Prosser, D. C., Whitworth, K., & Wendland, B. (2010) · Traffic : the International Journal of Intracellular Transport · doi:10.1111/j.1600-0854.2010.01088.x · Cited by 59
Plant dynamin-related protein families DRP1 and DRP2 in plant development
Bednarek, S. Y., & Backues, S. K. (2010) · Biochemical Society Transactions · doi:10.1042/bst0380797 · Cited by 58
The WD40 and FYVE domain containing protein 2 defines a class of early endosomes necessary for endocytosis
Hayakawa, A., Leonard, D. M., Murphy, S. T., Hayes, S. J., Soto, M. C., Fogarty, K., Standley, C., Bellve, K., Lambright, D., Mello, C., et al. (2006) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.0508832103 · Cited by 58
Spatial and Temporal Regulation of Receptor Endocytosis in Neuronal Dendrites Revealed by Imaging of Single Vesicle Formation
Rosendale, M., Jullié, D., Choquet, D., & Perrais, D. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.01.081 · Cited by 73
“However, as opposed to what had previously been observed in other cell types (Merrifield et al., 2005), the fluorescence at pH 7.”
InfluentialSelf-citation
Heat shock cognate protein 70 isoform D is required for clathrin-dependent endocytosis of Japanese encephalitis virus in C6/36 cells.
Chuang, C.-K., Yang, T.-H., Chen, T.-H., Yang, C.-F., & Chen, W.-J. (2015) · Journal of General Virology · doi:10.1099/jgv.0.000015 · Cited by 57
“In turn, the receptor-mediated endocytosis of extracellular molecules requires the involvement of clathrin that is highly dynamic and can be recycled after each round of endocytosis (McMahon & Boucrot, 2011; Merrifield et al., 2005).”
Background
Escherichia coli Producing CNF1 Toxin Hijacks Tollip to Trigger Rac1‐Dependent Cell Invasion
Visvikis, O., Boyer, L., Torrino, S., Doye, A., Lemonnier, M., Lorès, P., Rolando, M., Flatau, G., Mettouchi, A., Bouvard, D., et al. (2011) · Traffic · doi:10.1111/j.1600-0854.2011.01174.x · Cited by 58
Clathrin‐mediated endocytosis: What works for small, also works for big
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Mechanical requirements for membrane fission: Common facts from various examples
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Dynamin participates in the maintenance of anterior polarity in the C. elegans embryo
Nakayama, Y., Shivas, J. M., Poole, D. S., Squirrell, J., Kulkoski, J. M., Schleede, J. B., & Skop, A. R. (2009) · Developmental Cell · doi:10.1016/j.devcel.2009.04.009 · Cited by 57
“Given that dynamin can regulate both endocytic and actin dynamics in animal cells, and that actin regulators play a central role in endocytosis (Kaksonen et al., 2006; Merrifield, 2004; Merrifield et al., 2002, 2005; Smythe and Figure 5 .”
Background
Rho/ROCK and myosin II control the polarized distribution of endocytic clathrin structures at the uropod of moving T lymphocytes
Samaniego, R., Sánchez-Martı́n, L., Estecha, A., & Sánchez‐Mateos, P. (2007) · Journal of Cell Science · doi:10.1242/jcs.006296 · Cited by 50
“However, CSs also include a population of larger and more complex structures that may support multiple rounds of vesicle formation before being disassembled and correspond to platforms for cargo selection (Merrifield et al., 2005; Perrais and Merrifield, 2005).”
Background
Endocytosis in the axon initial segment maintains neuronal polarity
Eichel, K., Uenaka, T., Belapurkar, V., Lu, R., Cheng, S.-Q., Pak, J. S., Taylor, C. A., Südhof, T., Malenka, R., Wernig, M., et al. (2022) · Nature · doi:10.1038/s41586-022-05074-5 · Cited by 56
“The TfR-SEP plasmid was used 42 and described 60 previously.”
Self-citation

Authors: Merrifield, C. J., Perrais, D., & Zenisek, D.
Affiliations: MRC Laboratory of Molecular Biology (GB)
Centre National de la Recherche Scientifique (FR)
Yale University (US)
Corresponding author: Merrifield, Christien J.
ORCID: Perrais, David
Zenisek, David
Venue: Cell
Publisher: Elsevier BV
Published: 2005-05
Volume: 121
Issue: 4
Pages: 593-606
ISSN: 0092-8674 (ISSN-L)
DOI: 10.1016/j.cell.2005.03.015
OpenAlex: W2001532503
Cited by: 476
References: 0
Citations by year: 2026: 3 · 2025: 6 · 2024: 6 · 2023: 14 · 2022: 15 · 2021: 10 · 2020: 9 · 2019: 11 · 2018: 18 · 2017: 17 · 2016: 13 · 2015: 21 · 2014: 36 · 2013: 12 · 2012: 34
FWCI: 16.53
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 3030 d)
Locations: Cell (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords: clathrin-mediated endocytosis, membrane fission, clathrin-coated vesicles, cortactin, actin polymerization, latrunculin B, vesicle formation, dynamin, F-actin
MeSH terms: Actins, Animals, Cell Membrane, Coated Pits, Cell-Membrane, Endocytosis, Microfilament Proteins, Microscopy, Fluorescence, Polymers, Thiazoles, Microscopy, Video, Bridged Bicyclo Compounds, Heterocyclic, Clathrin-Coated Vesicles, Swiss 3T3 Cells, Cortactin, Mice, Thiazolidines

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Coupling between Clathrin-Coated-Pit Invagination, Cortactin Recruitment, and Membrane Scission Observed in Live Cells

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