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Molecular Anatomy of a Trafficking Organelle
Open accessAbstract
Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, and many of the proteins involved in these events are now known. However, a detailed picture of an entire trafficking organelle is not yet available. Using synaptic vesicles as a model, we have now determined the protein and lipid composition; measured vesicle size, density, and mass; calculated the average protein and lipid mass per vesicle; and determined the copy number of more than a dozen major constituents. A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins. Synaptic vesicles are dominated by proteins, possess a surprising diversity of trafficking proteins, and, with the exception of the V-ATPase that is present in only one to two copies, contain numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.
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“Competition for Rab6A on the vesicle will be high as the absolute number of Rab GTPases on an individual transport vesicle is likely to be small (∼10 per vesicle; Takamori et al., 2006) and many effectors, including two domains of KIF1C, will be vying to bind to Rabs.”
From The Rab6-regulated KIF1C kinesin motor domain contributes to Golgi organization (2015) · cites this paper as Takamori et al., 2006
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“The dendritic membranes on which these receptors reside are, similar to other biological membranes, highly crowded structures (Takamori et al., 2006).”
From Barriers in the brain: resolving dendritic spine morphology and compartmentalization (2014) · cites this paper as Takamori et al., 2006
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Membrane lipids: where they are and how they behave
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Metadata
- Authors
- Takamori, S., Holt, M., Stenius, K., Lemke, E. A., Grønborg, M., Riedel, D., Urlaub, H., Schenck, S., Brügger, B., Ringler, P., Müller, S. A., Rammner, B., Gräter, F., Hub, J. S., De Groot, B. L., Mieskes, G., Moriyama, Y., Klingauf, J., Grubmüller, H., Heuser, J., Wieland, F., & Jahn, R.
- Affiliations
- Max Planck Institute for Biophysical Chemistry (DE)
- Yale University (US)
- Heidelberg University (DE)
- University of Basel (CH)
- Okayama University (JP)
- Washington University in St. Louis (US)
- Corresponding author
- Jahn, Reinhard
- ORCID
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2006-11
- Volume
- 127
- Issue
- 4
- Pages
- 831-846
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2144447780
- Cited by
- 2,373
- References
- 65
- Citations by year
- 2026: 65 · 2025: 94 · 2024: 124 · 2023: 103 · 2022: 136 · 2021: 131 · 2020: 132 · 2019: 106 · 2018: 134 · 2017: 126 · 2016: 121 · 2015: 127 · 2014: 131 · 2013: 119 · 2012: 124
- FWCI
- 47.65
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Lirias (submittedVersion, other-oa)
- Max Planck Institute for Plasma Physics (submittedVersion)
- GRO (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion, other-oa)
- VUBIR (Vrije Universiteit Brussel) (submittedVersion)
- Funders
- University of Cambridge
- Deutsche Forschungsgemeinschaft
- Forsknings- og Innovationsstyrelsen
- Leibniz-Gemeinschaft
- Japan Society for the Promotion of Science
- Danish Agency for Science and Higher Education
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- synaptic vesicles, membrane trafficking, vesicle budding, vesicle fusion, protein composition, lipid composition, protein copy number, V-ATPase, neurotransmitter uptake
- MeSH terms
- Animals, Lipids, Models, Molecular, Proteins, Spectrometry, Fluorescence, Synaptic Vesicles, Protein Transport, Rats
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