Global Analysis of mRNA Localization Reveals a Prominent Role in Organizing Cellular Architecture and Function
Open accessAbstract
Although subcellular mRNA trafficking has been demonstrated as a mechanism to control protein distribution, it is generally believed that most protein localization occurs subsequent to translation. To address this point, we developed and employed a high-resolution fluorescent in situ hybridization procedure to comprehensively evaluate mRNA localization dynamics during early Drosophila embryogenesis. Surprisingly, of the 3370 genes analyzed, 71% of those expressed encode subcellularly localized mRNAs. Dozens of new and striking localization patterns were observed, implying an equivalent variety of localization mechanisms. Tight correlations between mRNA distribution and subsequent protein localization and function, indicate major roles for mRNA localization in nucleating localized cellular machineries. A searchable web resource documenting mRNA expression and localization dynamics has been established and will serve as an invaluable tool for dissecting localization mechanisms and for predicting gene functions and interactions.
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In early Drosophila embryo data sets measuring translation, stability, and localization, the authors obtained dozens of significant associations between the presence of motif matches and specific regulatory outcomes.
“we have examined data sets measuring translation40, stability41 and localization42 of transcripts in the early Drosophila embryo, obtaining dozens of significant associations between the presence of motif matches and specific regulatory outcomes”
From A compendium of RNA-binding motifs for decoding gene regulation (2013) · cites this paper as [42]
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- Authors
- Lécuyer, E., Yoshida, H., Parthasarathy, N., Alm, C., Babak, T., Cerovina, T., Hughes, T. R., Tomancak, P., & Krause, H. M.
- Affiliations
- University of Toronto (CA)
- Corresponding author
- Krause, Henry M.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2007-10
- Volume
- 131
- Issue
- 1
- Pages
- 174-187
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2163482144
- Cited by
- 967
- References
- 72
- Citations by year
- 2026: 17 · 2025: 38 · 2024: 43 · 2023: 43 · 2022: 49 · 2021: 49 · 2020: 50 · 2019: 46 · 2018: 52 · 2017: 61 · 2016: 62 · 2015: 49 · 2014: 73 · 2013: 63 · 2012: 58
- FWCI
- 20.02
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 2152 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Funders
- GAVI Alliance
- Adobe Systems
- Canadian Institutes of Health Research
- National Cancer Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- mRNA localization, Drosophila embryogenesis, protein localization, fluorescence in situ hybridization, cellular architecture, gene function prediction
- MeSH terms
- Animals, Cell Division, Cell Nucleus, Drosophila melanogaster, Embryo, Nonmammalian, RNA, Messenger, In Situ Hybridization, Fluorescence, Gene Expression Regulation, Developmental, Gene Expression Profiling, Drosophila Proteins, Databases, Nucleic Acid
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