The DOI matches its Crossref record; 64 of the 64 references it cites were found in a registry or an index.
Protein Architecture of the Human Kinetochore Microtubule Attachment Site
Open accessAbstract
Centromeric chromatin – spindle microtubule interactions mediated by kinetochores drive chromosome segregation. We have developed a two-color fluorescence light microscopy method that measures average label separation, Delta, at < 5 nm accuracy — to elucidate the protein architecture of human metaphase kinetochores. Delta analysis, when correlated with tension states of spindle-attached sister kinetochore pairs, provided information on mechanical properties of protein linkages within kinetochores. Treatment with taxol—which suppresses microtubule dynamics, eliminates tension at kinetochores, and activates the spindle checkpoint—resulted in specific large-scale changes in kinetochore architecture. Cumulatively, Delta analysis revealed compliant linkages close to the centromeric chromatin, suggests a model for how the KMN (KNL1/Mis12 complex/Ndc80 complex) network provides microtubule attachment and generates pulling forces from depolymerization, and reveals architectural changes induced by taxol treatment. The methods described here should also be applicable to other intermediate-scale biological machines in cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The spindle-assembly checkpoint in space and time
Musacchio, A., & Salmon, E. D. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2163 · Cited by 2,227
Self-citationThe Conserved KMN Network Constitutes the Core Microtubule-Binding Site of the Kinetochore
Cheeseman, I. M., Chappie, J. S., Wilson-Kubalek, E. M., & Desai, A. (2006) · Cell · doi:10.1016/j.cell.2006.09.039 · Cited by 994
Self-citationMolecular architecture of the kinetochore–microtubule interface
Cheeseman, I. M., & Desai, A. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2310 · Cited by 915
Self-citationKinetochore Microtubule Dynamics and Attachment Stability Are Regulated by Hec1
DeLuca, J. G., Gall, W. E., Ciferri, C., Cimini, D., Musacchio, A., & Salmon, E. D. (2006) · Cell · doi:10.1016/j.cell.2006.09.047 · Cited by 747
Self-citationThe human CENP-A centromeric nucleosome-associated complex
Foltz, D. R., Jansen, L. E. T., Black, B. E., Bailey, A. O., Yates, J. R., & Cleveland, D. W. (2006) · Nature Cell Biology · doi:10.1038/ncb1397 · Cited by 731
Temporal and spatial control of cyclin B1 destruction in metaphase
Clute, P., & Pines, J. (1999) · Nature Cell Biology · doi:10.1038/10049 · Cited by 702
Cimini, D., Howell, B., Maddox, P., Khodjakov, A., Degrassi, F., & Salmon, E. D. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.153.3.517 · Cited by 568
Self-citationConserved Organization of Centromeric Chromatin in Flies and Humans
Blower, M. D., Sullivan, B. A., & Karpen, G. H. (2002) · Developmental Cell · doi:10.1016/s1534-5807(02)00135-1 · Cited by 543
Force Generation by Microtubule Assembly/Disassembly in Mitosis and Related Movements
Inoué, S., & Salmon, E. D. (1995) · Molecular Biology of the Cell · doi:10.1091/mbc.6.12.1619 · Cited by 532
Self-citationCiferri, C., Pasqualato, S., Screpanti, E., Varetti, G., Santaguida, S., Dos Reis, G., Maiolica, A., Polka, J., De Luca, J. G., De Wulf, P., et al. (2008) · Cell · doi:10.1016/j.cell.2008.03.020 · Cited by 528
Self-citation
Cited by
A Molecular View of Kinetochore Assembly and Function
Musacchio, A., & Desai, A. (2017) · Biology · doi:10.3390/biology6010005 · Cited by 544
Self-citationSantaguida, S., Tighe, A., D'Alise, A. M., Taylor, S. S., & Musacchio, A. (2010) · Journal of Cell Biology · doi:10.1083/jcb.201001036 · Cited by 531
Welburn, J. P. I., Vleugel, M., Liu, D., Yates, J. R., Lampson, M. A., Fukagawa, T., & Cheeseman, I. M. (2010) · Molecular Cell · doi:10.1016/j.molcel.2010.02.034 · Cited by 481
Sensing centromere tension: Aurora B and the regulation of kinetochore function
Lampson, M. A., & Cheeseman, I. M. (2011) · Trends in Cell Biology · doi:10.1016/j.tcb.2010.10.007 · Cited by 375
Cheeseman, I. M. (2014) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a015826 · Cited by 298
Metadata
- Authors
- Wan, X., O'Quinn, R. P., Pierce, H. L., Joglekar, A. P., Gall, W. E., DeLuca, J. G., Carroll, C. W., Liu, S.-T., Yen, T. J., McEwen, B. F., Stukenberg, P. T., Desai, A., & Salmon, E. D.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2009-05
- Volume
- 137
- Issue
- 4
- Pages
- 672-684
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2171845884
- Cited by
- 334
- References
- 64
- FWCI
- 16.24
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Protein Architecture of the Human Kinetochore Microtubule Attachment Site. https://app.cochat.ai/papers/10.1016/j.cell.2009.03.035
- The paper
- Wan, X., O'Quinn, R. P., Pierce, H. L., Joglekar, A. P., Gall, W. E., DeLuca, J. G., Carroll, C. W., Liu, S.-T., Yen, T. J., McEwen, B. F., Stukenberg, P. T., Desai, A., & Salmon, E. D. (2009). Protein Architecture of the Human Kinetochore Microtubule Attachment Site. Cell. https://doi.org/10.1016/j.cell.2009.03.035
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
