The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering
Open accessAbstract
Mice carrying mutations in multiple genes are traditionally generated by sequential recombination in embryonic stem cells and/or time-consuming intercrossing of mice with a single mutation. The CRISPR/Cas system has been adapted as an efficient gene-targeting technology with the potential for multiplexed genome editing. We demonstrate that CRISPR/Cas-mediated gene editing allows the simultaneous disruption of five genes (Tet1, 2, 3, Sry, Uty--8 alleles) in mouse embryonic stem (ES) cells with high efficiency. Coinjection of Cas9 mRNA and single-guide RNAs (sgRNAs) targeting Tet1 and Tet2 into zygotes generated mice with biallelic mutations in both genes with an efficiency of 80%. Finally, we show that coinjection of Cas9 mRNA/sgRNAs with mutant oligos generated precise point mutations simultaneously in two target genes. Thus, the CRISPR/Cas system allows the one-step generation of animals carrying mutations in multiple genes, an approach that will greatly accelerate the in vivo study of functionally redundant genes and of epistatic gene interactions.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“Soon, all sorts of cells and some multicellular organisms would be the object of CRISPR-mediated manipulation, such as human cell cultures, mice, plants, yeasts, and the list goes on [62,63,64,65].”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [63]
The cited source says what the claim attributes to it.
In the citing paper
“The need for specific proteins tailored for each gene also makes multiplex gene editing a strenuous task for ZFNs and TALENs, while with Cas9 systems multiple genes can be targeted simply by delivering multiple gRNAs to the cells [63,98].”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [63]
The cited source says what the claim attributes to it.
In the citing paper
“focused on the issue of human germline engineering, as the methods have already been demonstrated in mice (6) and monkeys (7).”
From A prudent path forward for genomic engineering and germline gene modification (2015) · cites this paper as [6]
In the citing paper
“However, the desired recombination efficacy often occurs infrequently, which presents enormous challenges for robust applications [61, 82–84].”
From Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities (2018) · cites this paper as [61]
In the citing paper
“Recently, the CRISPR/Cas system has been successfully applied to human cells19,20,21,22 and several model organisms for genome editing.23,24,25,26”
From The CRISPR/Cas9 system facilitates clearance of the intrahepatic HBV templates in vivo (2014) · cites this paper as [26]
In the citing paper
“DNA sequence recognition requires only a single 20-nt guide RNA and a protospacer adjacent motif, which enables one to rapidly engineer and test a large number of DNA cleavage sites (17)(18)(19)(20).”
From RNA-guided endonuclease provides a therapeutic strategy to cure latent herpesviridae infection (2014) · cites this paper as [17]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
Self-citationRNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
Efficient genome editing in zebrafish using a CRISPR-Cas system
Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Tsai, S. Q., Sander, J. D., Peterson, R. T., Yeh, J.-R. J., & Joung, J. K. (2013) · Nature Biotechnology · doi:10.1038/nbt.2501 · Cited by 2,937
Gasiunas, G., Barrangou, R., Horvath, P., & Siksnys, V. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1208507109 · Cited by 2,769
CRISPR/Cas, the Immune System of Bacteria and Archaea
Horvath, P., & Barrangou, R. (2010) · Science · doi:10.1126/science.1179555 · Cited by 2,471
RNA-guided editing of bacterial genomes using CRISPR-Cas systems
Jiang, W., Bikard, D., Cox, D., Zhang, F., & Marraffini, L. A. (2013) · Nature Biotechnology · doi:10.1038/nbt.2508 · Cited by 2,429
Self-citationGenome editing with engineered zinc finger nucleases
Urnov, F. D., Rebar, E. J., Holmes, M. C., Zhang, H. S., & Gregory, P. D. (2010) · Nature Reviews Genetics · doi:10.1038/nrg2842 · Cited by 2,285
Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease
Cho, S. W., Kim, S., Kim, J. M., & Kim, J.-S. (2013) · Nature Biotechnology · doi:10.1038/nbt.2507 · Cited by 1,863
RNA-guided genetic silencing systems in bacteria and archaea
Wiedenheft, B., Sternberg, S. H., & Doudna, J. A. (2012) · Nature · doi:10.1038/nature10886 · Cited by 1,851
Flagged first, then by citations
Cited by
Sorted by citations
Items 701–800 of 1,005
Fukuhara, T., Wada, M., Nakamura, S., Ono, C., Shiokawa, M., Yamamoto, S., Motomura, T., Okamoto, T., Okuzaki, D., Yamamoto, M., et al. (2014) · PLoS Pathogens · doi:10.1371/journal.ppat.1004534 · Cited by 85
Didion, J. P., Morgan, A. P., Clayshulte, A. M.-F., McMullan, R., Yadgary, L., Petkov, P. M., Bell, T. A., Gatti, D. M., Crowley, J. J., Hua, K., et al. (2015) · PLoS Genetics · doi:10.1371/journal.pgen.1004850 · Cited by 83
Modeling psychiatric disorders: from genomic findings to cellular phenotypes
Falk, A., Heine, V. M., Harwood, A. J., Sullivan, P. F., Peitz, M., Brüstle, O., Shen, S., Sun, Y.-M., Glover, J. C., Posthuma, D., et al. (2016) · Molecular Psychiatry · doi:10.1038/mp.2016.89 · Cited by 88
Efficient generation of conditional knockout mice via sequential introduction of lox sites
Horii, T., Morita, S., Kimura, M., Terawaki, N., Shibutani, M., & Hatada, I. (2017) · Scientific Reports · doi:10.1038/s41598-017-08496-8 · Cited by 85
Generation of beta-lactoglobulin knock-out goats using CRISPR/Cas9
Zhou, W., Wan, Y., Guo, R., Deng, M., Deng, K., Wang, Z., Zhang, Y., & Wang, F. (2017) · PLoS ONE · doi:10.1371/journal.pone.0186056 · Cited by 89
Targeting fidelity of adenine and cytosine base editors in mouse embryos
Lee, H., Willi, M., Miller, S. M., Kim, S., Liu, C., Liu, D. R., & Hennighausen, L. G. (2018) · Nature Communications · doi:10.1038/s41467-018-07322-7 · Cited by 82
Zhu, Y. (2022) · BioMed Research International · doi:10.1155/2022/9978571 · Cited by 103
The Mouse as a Model for Age-Related Hearing Loss - A Mini-Review
Bowl, M. R., & Dawson, S. J. (2014) · Gerontology · doi:10.1159/000368399 · Cited by 85
CRISPR/Cas9‐Directed Genome Editing of Cultured Cells
Yang, L., Yang, J. L., Byrne, S., Pan, J., & Church, G. M. (2014) · Current Protocols in Molecular Biology · doi:10.1002/0471142727.mb3101s107 · Cited by 82
Ou, Z., Niu, X., He, W., Chen, Y., Song, B., Xian, Y., Fan, D., Tang, D., & Sun, X. (2016) · Scientific Reports · doi:10.1038/srep32463 · Cited by 84
iTAP, a novel iRhom interactor, controls TNF secretion by policing the stability of iRhom/TACE
Oikonomidi, I., Burbridge, E., Cavadas, M. A. S., Sullivan, G. P., Sengerová, B., Naegele, H., Clancy, D. M., Březinová, J., Hu, T., Bileck, A., et al. (2018) · eLife · doi:10.7554/elife.35032 · Cited by 87
Fang, S., Li, J., Xiao, Y., Lee, M., Guo, L., Han, W., Li, T., Hill, M. C., Hong, T., Mo, W., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-12325-z · Cited by 89
Modifying the lipid content and composition of plant seeds: engineering the production of LC-PUFA
Ruiz‐López, N., Usher, S. L., Sayanova, O. V., Napier, J. A., & Haslam, R. P. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-6217-2 · Cited by 92
Hu, Z. M., Shi, Z., Guo, X., Jiang, B., Wang, G., Luo, D., Chen, Y., & Zhu, Y. (2018) · Cell & Bioscience · doi:10.1186/s13578-018-0200-z · Cited by 83
Codner, G. F., Mianné, J., Caulder, A., Loeffler, J., Fell, R., King, R., Allan, A. J., Mackenzie, M., Pike, F. J., McCabe, C. V., et al. (2018) · BMC Biology · doi:10.1186/s12915-018-0530-7 · Cited by 84
Huang, N., Zhang, D., Li, F., Chai, P., Wang, S., Teng, J., & Chen, J. (2018) · Nature Communications · doi:10.1038/s41467-018-06990-9 · Cited by 83
Schizophrenia and Depression Co-Morbidity: What We have Learned from Animal Models
Samsom, J., & Wong, A. H. C. (2015) · Frontiers in Psychiatry · doi:10.3389/fpsyt.2015.00013 · Cited by 88
Mianné, J., Codner, G. F., Caulder, A., Fell, R., Hutchison, M., King, R., Stewart, M. E., Wells, S. E., & Teboul, L. (2017) · Methods · doi:10.1016/j.ymeth.2017.03.016 · Cited by 83
El‐Sayed, A. S. A., Abdel‐Ghany, S. E., & Ali, G. S. (2017) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-017-8263-z · Cited by 86
Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes
Mashiko, D., Young, S. A. M., Muto, M., Kato, H., Nozawa, K., Ogawa, M., Noda, T., Kim, Y., Satouh, Y., Fujihara, Y., et al. (2013) · Development Growth & Differentiation · doi:10.1111/dgd.12113 · Cited by 82
Palmiero, U. C., Kaczmarek, J. C., Fenton, O. S., & Anderson, D. G. (2018) · Advanced Healthcare Materials · doi:10.1002/adhm.201800249 · Cited by 84
Highly multiplexed genome engineering using CRISPR/Cas9 gRNA arrays
Kurata, M., Wolf, N. K., Lahr, W. S., Weg, M. T., Kluesner, M. G., Lee, S. A., Hui, K., Shiraiwa, M., Webber, B. R., & Moriarity, B. S. (2018) · PLoS ONE · doi:10.1371/journal.pone.0198714 · Cited by 89
Allen, D. G., Rosenberg, M., & Hendel, A. (2021) · Frontiers in Genome Editing · doi:10.3389/fgeed.2020.617910 · Cited by 89
An optimized CRISPR/Cas9 approach for precise genome editing in neurons
Fang, H., Bygrave, A. M., Roth, R. H., Johnson, R. C., & Huganir, R. L. (2021) · eLife · doi:10.7554/elife.65202 · Cited by 81
The tracrRNA in CRISPR Biology and Technologies
Liao, C., & Beisel, C. L. (2021) · Annual Review of Genetics · doi:10.1146/annurev-genet-071719-022559 · Cited by 91
Efficient Gene Targeting in Golden Syrian Hamsters by the CRISPR/Cas9 System
Fan, Z., Li, W., Lee, S. R., Meng, Q., Shi, B., Bunch, T. D., White, K. L., Kong, I., & Wang, Z. (2014) · PLoS ONE · doi:10.1371/journal.pone.0109755 · Cited by 80
The fidelity of the ligation step determines how ends are resolved during nonhomologous end joining
Waters, C. A., Strande, N. T., Pryor, J. M., Strom, C. N., Mieczkowski, P., Burkhalter, M. D., Oh, S., Qaqish, B. F., Moore, D. T., Hendrickson, E. A., et al. (2014) · Nature Communications · doi:10.1038/ncomms5286 · Cited by 78
Evolution of transcriptional enhancers and animal diversity
Rubinstein, M., & de Souza, F. S. J. (2013) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2013.0017 · Cited by 79
Sui generis: gene therapy and delivery systems for the treatment of glioblastoma
KANE, J. R., Miska, J. M., Young, J. S., Kanojia, D., Kim, J. W., & Lesniak, M. S. (2015) · Neuro-Oncology · doi:10.1093/neuonc/nou355 · Cited by 82
Zhou, J., Wu, R., Xue, X., & Qin, Z. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw475 · Cited by 82
The contribution of branching morphogenesis to kidney development and disease
Short, K. M., & Smyth, I. (2016) · Nature Reviews Nephrology · doi:10.1038/nrneph.2016.157 · Cited by 83
Gene Editing With CRISPR/Cas9 RNA-Directed Nuclease
Doetschman, T., & Georgieva, T. G. (2017) · Circulation Research · doi:10.1161/circresaha.116.309727 · Cited by 91
Williams, R. M., Senanayake, U., Artibani, M., Taylor, G., Wells, D., Ahmed, A., & Sauka-Spengler, T. (2018) · Development · doi:10.1242/dev.160333 · Cited by 77
The genetics of Crohn’s disease and ulcerative colitis –status quoand beyond
Ellinghaus, D., Bethune, J., Petersen, B., & Franke, A. (2014) · Scandinavian Journal of Gastroenterology · doi:10.3109/00365521.2014.990507 · Cited by 80
Zhong, H., Chen, Y., Li, Y., Chen, R., & Mardon, G. (2015) · Scientific Reports · doi:10.1038/srep08366 · Cited by 81
Exciting Prospects for Precise Engineering of Caenorhabditis elegans Genomes with CRISPR/Cas9
Frøkjær‐Jensen, C. (2013) · Genetics · doi:10.1534/genetics.113.156521 · Cited by 79
Genome editing methods in animal models
Lee, H., Yoon, D. E., & Kim, K. (2020) · Animal Cells and Systems · doi:10.1080/19768354.2020.1726462 · Cited by 83
Germline transgenesis in rabbits by pronuclear microinjection of Sleeping Beauty transposons
Ivics, Z., Hiripi, L., Hoffmann, O. I., Mátés, L., Yau, T. Y., Bashir, S., Zídek, V., Landa, V., Geurts, A. M., Pravenec, M., et al. (2014) · Nature Protocols · doi:10.1038/nprot.2014.009 · Cited by 81
CRISPR/Cas9 Nuclease Cleavage Combined with Gibson Assembly for Seamless Cloning
Wang, J., Wang, A., Li, K., Wang, B., Jin, S., Reiser, M. A., & Lockey, R. F. (2015) · BioTechniques · doi:10.2144/000114261 · Cited by 80
Genetic and epigenetic control of gene expression by CRISPR–Cas systems
Lo, A., & Qi, L. (2017) · F1000Research · doi:10.12688/f1000research.11113.1 · Cited by 72
Raveux, A., Vandormael‐Pournin, S., & Cohen‐Tannoudji, M. (2017) · Scientific Reports · doi:10.1038/srep42661 · Cited by 81
Ye, L., Wang, C., Hong, L., Sun, N., Chen, D., Chen, S., & Han, F. (2018) · Cell Discovery · doi:10.1038/s41421-018-0049-7 · Cited by 83
TMCO1-mediated Ca2+ leak underlies osteoblast functions via CaMKII signaling
Li, J., Liu, C., Li, Y., Zheng, Q., Xu, Y., Liu, B., Sun, W., Ji, S., Ji, S., Liu, M., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-09653-5 · Cited by 80
Kim, H., Choi, J., & Won, K. (2020) · BMC Plant Biology · doi:10.1186/s12870-020-02665-0 · Cited by 82
Serneels, L., T’Syen, D., Pérez‐Benito, L., Theys, T., Holt, M. G., & Strooper, B. D. (2020) · Molecular Neurodegeneration · doi:10.1186/s13024-020-00399-z · Cited by 78
Germline genome-editing research and its socioethical implications
Ishii, T. (2015) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2015.05.006 · Cited by 86
Kumar, N., Stanford, W., de Solis, C., Aradhana, Abraham, N. D., Dao, T.-M. J., Thaseen, S., Sairavi, A., Gonzalez, C. U., & Ploski, J. E. (2018) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2018.00413 · Cited by 79
Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles
Lanza, D. G., Gaspero, A., Lorenzo, I., Liao, L., Zheng, P., Wang, Y., Deng, Y., Cheng, C., Zhang, C., Rasband, M. N., et al. (2018) · BMC Biology · doi:10.1186/s12915-018-0529-0 · Cited by 78
Mesubi, O. O., Rokita, A. G., Abrol, N., Wu, Y., Chen, B., Wang, Q., Granger, J. M., Tucker-Bartley, A., Luczak, E. D., Murphy, K. R., et al. (2020) · Journal of Clinical Investigation · doi:10.1172/jci95747 · Cited by 82
Molecular scissors and their application in genetically modified farm animals
Petersen, B., & Niemann, H. (2015) · Transgenic Research · doi:10.1007/s11248-015-9862-z · Cited by 79
CRISPR/Cas9-mediated gene knockout in the mouse brain using in utero electroporation
Shinmyo, Y., Tanaka, S., Tsunoda, S., Hosomichi, K., Tajima, A., & Kawasaki, H. (2016) · Scientific Reports · doi:10.1038/srep20611 · Cited by 81
Virus-Like Particle Mediated CRISPR/Cas9 Delivery for Efficient and Safe Genome Editing
Lyu, P., Wang, L., & Lu, B. (2020) · Life · doi:10.3390/life10120366 · Cited by 79
MIC-Drop: A platform for large-scale in vivo CRISPR screens
Parvez, S., Herdman, C., Beerens, M., Chakraborti, K., Harmer, Z. P., Yeh, J.-R. J., MacRae, C. A., Yost, H. J., & Peterson, R. T. (2021) · Science · doi:10.1126/science.abi8870 · Cited by 77
Horie, K., Inoue, K., Suzuki, S., Adachi, S., Yada, S., Hirayama, T., Hidema, S., Young, L. J., & Nishimori, K. (2019) · Hormones and Behavior · doi:10.1016/j.yhbeh.2018.10.011 · Cited by 77
Genome-wide association study identifies two risk loci for tuberculosis in Han Chinese
Zheng, R., Li, Z., He, F., Liu, H., Chen, J., Chen, J., Xie, X., Zhou, J., Chen, H., Wu, X., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-06539-w · Cited by 76
Self-citationApplication of CRISPR/Cas9 gene editing technique in the study of cancer treatment
Jiang, C., Meng, L., Yang, B., & Luo, X. (2019) · Clinical Genetics · doi:10.1111/cge.13589 · Cited by 83
Evaluating the Efficiency of gRNAs in CRISPR/Cas9 Mediated Genome Editing in Poplars
Brügmann, T., Deecke, K., & Fladung, M. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20153623 · Cited by 77
CRISPR/Cas-Based Modifications for Therapeutic Applications: A Review
Bharathkumar, N., Abraham, S., Meera, P., Aksah, S., Muthu, K., Saravanan, K. M., & Anand, T. (2021) · Molecular Biotechnology · doi:10.1007/s12033-021-00422-8 · Cited by 80
Efficient Gene Disruption in Cultured Primary Human Endothelial Cells by CRISPR/Cas9
Abrahimi, P., Chang, W., Kluger, M. S., Qyang, Y., Tellides, G., Saltzman, W. M., & Pober, J. S. (2015) · Circulation Research · doi:10.1161/circresaha.117.306290 · Cited by 81
High-throughput mouse phenomics for characterizing mammalian gene function
Brown, S. D. M., Holmes, C. C., Mallon, A., Meehan, T. F., Smedley, D. P., & Wells, S. (2018) · Nature Reviews Genetics · doi:10.1038/s41576-018-0005-2 · Cited by 84
Yu, Z., Chen, H., Liu, J., Zhang, H., Yan, Y., Zhu, N., Guo, Y., Yang, B., Chang, Y. P., Dai, F., et al. (2014) · Biology Open · doi:10.1242/bio.20147682 · Cited by 76
Applications of CRISPR Genome Engineering in Cell Biology
Wang, F., & Qi, L. S. (2016) · Trends in Cell Biology · doi:10.1016/j.tcb.2016.08.004 · Cited by 79
Yoshino, H., Yonemori, M., Miyamoto, K., Tatarano, S., Kofuji, S., Nohata, N., Nakagawa, M., & Enokida, H. (2017) · Oncotarget · doi:10.18632/oncotarget.14930 · Cited by 79
Liu, Q., Qi, Y., Liang, Q., Song, J. T., Liu, J., Li, W., Shu, Y., Tao, M., Chun, Z., Qin, Q., et al. (2018) · Science China Life Sciences · doi:10.1007/s11427-018-9404-7 · Cited by 76
Multiplexed CRISPR-Cas9-Based Genome Editing of Rhodosporidium toruloides
Otoupal, P. B., Ito, M., Arkin, A. P., Magnuson, J. K., Gladden, J. M., & Skerker, J. M. (2019) · mSphere · doi:10.1128/msphere.00099-19 · Cited by 79
Rea, V., & Raay, T. J. V. (2020) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2020.575575 · Cited by 77
CRISPR/Cas9 Technology and Its Utility for Crop Improvement
Liu, H., Chen, W., Li, Y., Sun, L., Chai, Y., Chen, H., Nie, H., & Huang, C. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms231810442 · Cited by 85
CRISPR–Cas9 a boon or bane: the bumpy road ahead to cancer therapeutics
Ghosh, D., Venkataramani, P., Nandi, S., & Bhattacharjee, S. (2019) · Cancer Cell International · doi:10.1186/s12935-019-0726-0 · Cited by 83
Evaluation of sgRNA Target Sites for CRISPR-Mediated Repression of TP53
Lawhorn, I. E. B., Ferreira, J. P., & Wang, C. L. (2014) · PLoS ONE · doi:10.1371/journal.pone.0113232 · Cited by 75
Seruggia, D., & Montoliu, L. (2014) · Transgenic Research · doi:10.1007/s11248-014-9823-y · Cited by 79
Hominini-specific regulation of CBLN2 increases prefrontal spinogenesis
Shibata, M., Pattabiraman, K., Muchnik, S. K., Kaur, N., Morozov, Y. M., Cheng, X., Waxman, S. G., & Šestan, N. (2021) · Nature · doi:10.1038/s41586-021-03952-y · Cited by 79
Valletta, S., Dolatshad, H., Bartenstein, M., Yip, B. H., Bello, E., Gordon, S. A. K., Yu, Y., Shaw, J. M., Roy, S., Scifo, L., et al. (2015) · Oncotarget · doi:10.18632/oncotarget.6392 · Cited by 75
Genome Editing and Its Applications in Model Organisms
Ma, D., & Liu, F. (2015) · Genomics Proteomics & Bioinformatics · doi:10.1016/j.gpb.2015.12.001 · Cited by 81
CRISPR/Cas9-mediated somatic and germline gene correction to restore hemostasis in hemophilia B mice
Huai, C., Jia, C., Sun, R., Xu, P., Min, T., Wang, Q., Zheng, C., Chen, H., & Lu, D. (2017) · Human Genetics · doi:10.1007/s00439-017-1801-z · Cited by 76
Seruggia, D., Fernández, A., Cantero, M., Pelczar, P., & Montoliu, L. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv375 · Cited by 76
Factors controlling sperm migration through the oviduct revealed by gene-modified mouse models
Fujihara, Y., Miyata, H., & Ikawa, M. (2018) · EXPERIMENTAL ANIMALS · doi:10.1538/expanim.17-0153 · Cited by 74
Potential Application of the CRISPR/Cas9 System against Herpesvirus Infections
Chen, Y., Sheng, J., Trang, P., & Liu, F. (2018) · Viruses · doi:10.3390/v10060291 · Cited by 74
Unexpected consequences of genetic selection in broilers and turkeys: problems and solutions
Hocking, P. M. (2014) · British Poultry Science · doi:10.1080/00071668.2014.877692 · Cited by 91
Wijdeven, R. H. M., Pang, B., van der Zanden, S. Y., Qiao, X., Blomen, V. A., Hoogstraat, M., Lips, E. H., Janssen, L., Wessels, L. F. A., Brummelkamp, T. R., et al. (2015) · Cancer Research · doi:10.1158/0008-5472.can-15-0380 · Cited by 74
Mulder, C. L., Zheng, Y., Jan, S. Z., Struijk, R. B., Repping, S., Hamer, G., & van Pelt, A. M. M. (2016) · Human Reproduction Update · doi:10.1093/humupd/dmw017 · Cited by 76
Rational Design of Mouse Models for Cancer Research
Landgraf, M., McGovern, J., Friedl, P., & Hutmacher, D. W. (2018) · Trends in biotechnology · doi:10.1016/j.tibtech.2017.12.001 · Cited by 77
Rong, Z., Zhu, S., Xu, Y., & Fu, X. (2014) · Protein & Cell · doi:10.1007/s13238-014-0032-5 · Cited by 73
Clarke, B. A., Majumder, S., Zhu, H., Lee, Y. T., Kono, M., Li, C., Khanna, C., Blain, H., Wertman, R. S., Huso, V. L., et al. (2019) · eLife · doi:10.7554/elife.51067 · Cited by 73
Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences
Tavakoli, K., Pour‐Aboughadareh, A., Kianersi, F., Poczai, P., Etminan, A., & Shooshtari, L. (2021) · BioTech · doi:10.3390/biotech10030014 · Cited by 84
Wen, W., Quan, Z.-J., Li, S.-A., Yang, Z.-X., Fu, Y.-W., Zhang, F., Li, G., Zhao, M., Yin, M., Xu, J., et al. (2021) · Genome biology · doi:10.1186/s13059-021-02462-4 · Cited by 77
Self-citationCD24-Siglec axis is an innate immune checkpoint against metaflammation and metabolic disorder
Wang, X., Liu, M., Zhang, J., Brown, N. K., Zhang, P., Zhang, Y., Liu, H., Du, X., Wu, W., Devenport, M., et al. (2022) · Cell Metabolism · doi:10.1016/j.cmet.2022.07.005 · Cited by 74
Xie, G., Lee, J., Senft, A. D., Park, Y.-K., Jang, Y., Chakraborty, S., Thompson, J. J., McKernan, K. E., Liu, C., Macfarlan, T. S., et al. (2023) · Nature Genetics · doi:10.1038/s41588-023-01356-4 · Cited by 73
RETRACTED: microRNA-382 as a tumor suppressor? Roles in tumorigenesis and clinical significance
Fattahi, M., Shahrabi, S., Saadatpour, F., Rezaee, D., Beyglu, Z., Delavari, S., Amrolahi, A., Ahmadi, S., Bagheri‐Mohammadi, S., Noori, E., et al. (2023) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.125863 · Cited by 75
RetractedSato, M., Koriyama, M., Watanabe, S., Ohtsuka, M., Sakurai, T., Inada, E., Saitoh, I., Nakamura, S., & Miyoshi, K. (2015) · International Journal of Molecular Sciences · doi:10.3390/ijms160817838 · Cited by 72
A Guide RNA Sequence Design Platform for the CRISPR/Cas9 System for Model Organism Genomes
Ma, M., Ye, A. Y., Zheng, W., & Kong, L. (2013) · BioMed Research International · doi:10.1155/2013/270805 · Cited by 73
Square, T. A., Romášek, M., Jandzík, D., Cattell, M. V., Klymkowsky, M. W., & Medeiros, D. M. (2015) · Development · doi:10.1242/dev.125609 · Cited by 74
GPI-AP release in cellular, developmental, and reproductive biology
Fujihara, Y., & Ikawa, M. (2015) · Journal of Lipid Research · doi:10.1194/jlr.r063032 · Cited by 74
CRISPR-Cas9 technology: applications and human disease modelling
Torres-Ruiz, R., & Rodríguez, S. (2016) · Briefings in Functional Genomics · doi:10.1093/bfgp/elw025 · Cited by 77
de la Fuente‐Núñez, C., & Lu, T. K. (2016) · Integrative Biology · doi:10.1039/c6ib00140h · Cited by 75
Evaluation and rational design of guide RNAs for efficient CRISPR/Cas9-mediated mutagenesis in Ciona
Gandhi, S., Haeussler, M., Razy‐Krajka, F., Christiaen, L., & Stolfi, A. (2017) · Developmental Biology · doi:10.1016/j.ydbio.2017.03.003 · Cited by 67
Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editingPreprint
Wang, K., Jin, Q., Ruan, D., Yang, Y., Liu, Q., Wu, H., Zhou, Z., Ouyang, Z., Liu, Z., Zhao, Y., et al. (2017) · Genome Research · doi:10.1101/gr.222521.117 · Cited by 75
Toward stem cell-based phenotypic screens for neurodegenerative diseases
Khurana, V., Tardiff, D. F., Chung, C. Y., & Lindquist, S. L. (2015) · Nature Reviews Neurology · doi:10.1038/nrneurol.2015.79 · Cited by 72
Zhou, X., Wang, L., Du, Y., Xie, F., Li, L., Liu, Y., Liu, C., Wang, S., Zhang, S., Huang, X., et al. (2015) · Human Mutation · doi:10.1002/humu.22913 · Cited by 72
CRISPR/Cas9 Nuclease-Mediated Gene Knock-In in Bovine-Induced Pluripotent Cells
Heo, Y. T., Quan, X., Xu, Y. N., Baek, S., Choi, H., Kim, N.-H., & Kim, J. (2015) · Stem Cells and Development · doi:10.1089/scd.2014.0278 · Cited by 73
Bruder, M. R., Pyne, M. E., Moo‐Young, M., Chung, D. A., & Chou, C.-H. P. (2016) · Applied and Environmental Microbiology · doi:10.1128/aem.02128-16 · Cited by 78
Metadata
- Authors
- Wang, H., Yang, H., Shivalila, C. S., Dawlaty, M. M., Cheng, A. W., Zhang, F., & Jaenisch, R.
- Affiliations
- Whitehead Institute for Biomedical Research (US)
- Broad Institute (US)
- Corresponding author
- Jaenisch, Rudolf
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2013-05
- Volume
- 153
- Issue
- 4
- Pages
- 910-918
- ISSN
- 0092-8674 (ISSN-L)
- PMC
- PMC3969854
- OpenAlex
- W2170039745
- Cited by
- 3,495
- References
- 35
- Citations by year
- 2026: 63 · 2025: 94 · 2024: 166 · 2023: 203 · 2022: 210 · 2021: 239 · 2020: 286 · 2019: 299 · 2018: 332 · 2017: 352 · 2016: 392 · 2015: 362 · 2014: 359 · 2013: 134
- FWCI
- 186.33
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc-nd)
- PMC
- Funders
- Damon Runyon Cancer Research Foundation
- National Institutes of Health (2r01ca084198-10)
- National Institute of Mental Health (DP1MH100706)
- National Cancer Institute (R01-CA084198)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R37HD045022)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Keywords
- CRISPR/Cas genome editing, multiplexed genome editing, mouse mutagenesis, TET1, TET2, TET3, SRY, UTY, biallelic variants, single guide RNA, zygote injection, mouse embryonic stem cells
- MeSH terms
- Animals, Base Sequence, Female, Male, Mice, Inbred C57BL, Mice, Inbred DBA, Molecular Sequence Data, Gene Targeting, Mice, Embryonic Stem Cells, RNA, Small Untranslated
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
