Poly(ADP-ribosyl)ation regulates insulator function and intrachromosomal interactions in Drosophila
Open accessAbstract
Insulators mediate inter- and intrachromosomal contacts to regulate enhancer-promoter interactions and establish chromosome domains. The mechanisms by which insulator activity can be regulated to orchestrate changes in the function and three-dimensional arrangement of the genome remain elusive. Here, we demonstrate that Drosophila insulator proteins are poly(ADP-ribosyl)ated and that mutation of the poly(ADP-ribose) polymerase (Parp) gene impairs their function. This modification is not essential for DNA occupancy of insulator DNA-binding proteins dCTCF and Su(Hw). However, poly(ADP-ribosyl)ation of K566 in CP190 promotes protein-protein interactions with other insulator proteins, association with the nuclear lamina, and insulator activity in vivo. Consistent with these findings, the nuclear clustering of CP190 complexes is disrupted in Parp mutant cells. Importantly, poly(ADP-ribosyl)ation facilitates intrachromosomal interactions between insulator sites measured by 4C. These data suggest that the role of insulators in organizing the three-dimensional architecture of the genome may be modulated by poly(ADP-ribosyl)ation.
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CTCF binding to DNA can be modulated by post-translational modifications such as poly-ADP ribosylation.
“CTCF-binding to DNA can also be modulated by post-translational modifications such as poly-ADP ribosylation (Ong et al., 2013).”
From Insulated Neighborhoods: Structural and Functional Units of Mammalian Gene Control (2016) · cites this paper as Ong et al., 2013
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“In vertebrates, in addition to CTCF, TAD borders are enriched in SINE elements and tRNA genes (Dixon et al., 2012), which contain binding sites for the insulator protein TFIIIC (Van Bortle and Corces, 2012).”
P Element-Mediated Germ-Line Transformation of Drosophila
O’Connor, M. J., & Chia, W. N. (2003) · Humana Press eBooks · doi:10.1385/0-89603-245-0:75 · Cited by 46 · cited 2× · see citing works
“P-element-mediated transformation was carried out by injecting either WT or K566A mutant CP190 transgenes directly into y2wct6; CP190H312/Tm6b embryos together with D2-3 plasmid (O’Connor and Chia, 1993).”
Strödicke, M., Karberg, S., & Korge, G. (2000) · Mechanisms of Development · doi:10.1016/s0925-4773(00)00371-3 · Cited by 37 · see citing works
Nuclear Matrix Proteome Analysis of Drosophila melanogaster
Kallappagoudar, S., Varma, P., Pathak, R. U., Ramamoorthy, S., & Mishra, R. K. (2010) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m110.001362 · Cited by 36 · cited 3× · see citing works
“We detect enrichment of different insulator proteins in the nuclear matrix, which is characterized by the presence of Lamin Dm0 and the absence of histones (Figure 3B) (Kallappagoudar et al., 2010).”
InfluentialPoly(ADP‐ribose) polymerase‐1: Association with nuclear lamins in rodent liver cells
Vidaković, M., Grdović, N., Quesada, P., Bode, J., & Poznanović, G. (2004) · Journal of Cellular Biochemistry · doi:10.1002/jcb.20289 · Cited by 14 · see citing works
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Sorted by citations
CTCF: an architectural protein bridging genome topology and function
Ong, C., & Corces, V. G. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3663 · Cited by 1,044 · see citing works
PARPs and ADP-ribosylation: recent advances linking molecular functions to biological outcomes
Gupte, R., Liu, Z., & Kraus, W. L. (2017) · Genes & Development · doi:10.1101/gad.291518.116 · Cited by 681 · see citing works
“Loss of dPARP results in the disruption of the nuclear clustering of CP190 complexes and reduces insulator activity (Ong et al. 2013).”
BackgroundInsulated Neighborhoods: Structural and Functional Units of Mammalian Gene Control
Hnisz, D., Day, D. S., & Young, R. A. (2016) · Cell · doi:10.1016/j.cell.2016.10.024 · Cited by 420 · see citing works
“CTCF-binding to DNA can also be modulated by post-translational modifications such as poly-ADP ribosylation (Ong et al., 2013).”
Insulator function and topological domain border strength scale with architectural protein occupancy
Bortle, K. V., Nichols, M. H., Li, L., Ong, C., Takenaka, N., Qin, Z. S., & Corces, V. G. (2014) · Genome biology · doi:10.1186/gb-2014-15-5-r82 · Cited by 294 · see citing works
“A recent study further suggests that APBSs are regulated by poly(ADP-ribosyl) ation of CP190, particularly at low occupancy, independent APBSs [65], whereas high occupancy APBSs more often remain unaffected.”
BackgroundBeyond DNA Repair: Additional Functions of PARP-1 in Cancer
Weaver, A. N., & Yang, E. S. (2013) · Frontiers in Oncology · doi:10.3389/fonc.2013.00290 · Cited by 246 · see citing works
CTCF mediates chromatin looping via N-terminal domain-dependent cohesin retention
Pugacheva, E. M., Kubo, N., Loukinov, D. I., Tajmul, M., Kang, S., Kovalchuk, A. L., Strunnikov, A. V., Zentner, G. E., Ren, B., & Lobanenkov, V. V. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1911708117 · Cited by 215 · see citing works
“As some of these posttranslational modifications have been reported to be essential for CTCF insulator function (49, 50), we explored the role of the CTCF N terminus in cohesin retention by generating a set of four different mutant forms.”
BackgroundEpigenetic silencing of tumor suppressor genes: Paradigms, puzzles, and potential
Kazanets, A., Shorstova, T., Hilmi, K., Marques, M., & Witcher, M. (2016) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/j.bbcan.2016.04.001 · Cited by 199 · see citing works
“In Drosophila, such a loss of poly(ADP-ribosylation) results in a release of a fraction of total CTCF from chromatin [106].”
BackgroundZhao, H., Sifakis, E. G., Sumida, N., Millán-Ariño, L., Scholz, B. A., Svensson, J. P., Chen, X., Ronnegren, A. L., de Lima, C. D. M., Varnoosfaderani, F. S., et al. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.07.019 · Cited by 138 · see citing works
“The interactome uncovered here is therefore distinct from the CTCF-PAR regulated intra-chromosomal network reported in Drosophila (Ong et al., 2013).”
BackgroundJubin, T., Kadam, A. A., Jariwala, M., Bhatt, S., Sutariya, S., Gani, A. R., Gautam, S., & Begum, R. (2016) · Cell Proliferation · doi:10.1111/cpr.12268 · Cited by 130 · see citing works
Insulators and domains of gene expression
Ali, T., Renkawitz, R., & Bartkuhn, M. (2016) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2015.11.009 · Cited by 121 · see citing works
Vogelmann, J., Gall, A. L., Déjardin, S., Allemand, F., Gamot, A., Labesse, G., Cuvier, O., Negre, N. N., Cohen‐Gonsaud, M., Margeat, E., et al. (2014) · PLoS Genetics · doi:10.1371/journal.pgen.1004544 · Cited by 119 · see citing works
“In addition to interacting with BEAF32, CP190 is able to directly interact with other insulator binding proteins, such as dCTCF, Su(HW), and Mod(Mdg4) [21,39,66–68], or with the RNA interference machinery [69].”
BackgroundPARP1 orchestrates epigenetic events setting up chromatin domains
Ciccarone, F., Zampieri, M., & Caiafa, P. (2016) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2016.11.010 · Cited by 110 · see citing works
Kaushal, A., Mohana, G., Dorier, J., Özdemir, İ., Omer, A., Cousin, P., Semenova, A. I., Taschner, M., Dergai, O., Marzetta, F., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-21366-2 · Cited by 99 · see citing works
Architectural proteins: regulators of 3D genome organization in cell fate
Gómez‐Díaz, E., & Corces, V. G. (2014) · Trends in Cell Biology · doi:10.1016/j.tcb.2014.08.003 · Cited by 100 · see citing works
Enhancing NAD+ salvage metabolism is neuroprotective in a PINK1 model of Parkinson's disease
Lehmann, S., Loh, S. H. Y., & Martins, L. M. (2016) · Biology Open · doi:10.1242/bio.022186 · Cited by 95 · see citing works
“To address this, we examined whether a loss-of-function mutation in the Parp gene in ParpCH1/+ flies (Ong et al., 2013; Tulin et al., 2002; Zhang and Spradling, 1994) could reverse the mitochondrial impairment of pink1 mutants.”
Architectural proteins, transcription, and the three‐dimensional organization of the genome
Cubeñas-Potts, C., & Corces, V. G. (2015) · FEBS Letters · doi:10.1016/j.febslet.2015.05.025 · Cited by 87 · see citing works
PARP, transcription and chromatin modeling
Marjanović, M. P., Crawford, K., & Ahel, I. (2016) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2016.09.014 · Cited by 70 · see citing works
Lehmann, S., Costa, A. C., Celardo, I., Loh, S. H. Y., & Martins, L. M. (2016) · Cell Death & Disease · doi:10.1038/cddis.2016.72 · Cited by 69 · see citing works
The role of chromatin insulators in nuclear architecture and genome function
Bortle, K. V., & Corces, V. G. (2013) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2012.11.003 · Cited by 65 · see citing works
Essential role of Cp190 in physical and regulatory boundary formation
Kaushal, A., Dorier, J., Wang, B., Mohana, G., Taschner, M., Cousin, P., Waridel, P., Iseli, C., Semenova, A., Restrepo, S., et al. (2022) · Science Advances · doi:10.1126/sciadv.abl8834 · Cited by 61 · see citing works
Viets, K., Sauria, M. E. G., Chernoff, C., Viales, R. R., Echterling, M., Anderson, C., Tran, S., Dove, A., Goyal, R., Voortman, L., et al. (2019) · Developmental Cell · doi:10.1016/j.devcel.2019.09.007 · Cited by 58 · see citing works
“Using published ChIP data (Cuartero et al., 2014; Negre et al., 2010; Ong et al., 2013; Van Bortle et al., 2012; Wood et al., 2011), we found an association between pairing and the DNA-binding insulator proteins CTCF and GAF and insulator cofactors Cp190 and Mod(mdg4)(Fig.”
MethodRegulation of 3D chromatin organization by CTCF
Xiang, J., & Corces, V. G. (2020) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2020.10.005 · Cited by 58 · see citing works
“In Drosophila , where CTCF is also PARy-lated, this modification is required to facilitate interactions between distant sites [36].”
BackgroundBohla, D., Herold, M. V. B., Panzer, I., Buxa, M. K., Ali, T., Demmers, J., Krüger, M., Scharfe, M., Jarek, M., Bartkuhn, M., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0107765 · Cited by 57 · see citing works
Morgan, S. M., Tanizawa, H., Caruso, L. B., Hulse, M., Kossenkov, A. V., Madžo, J., Keith, K., Tan, Y., Boyle, S., Lieberman, P. M., et al. (2022) · Nature Communications · doi:10.1038/s41467-021-27894-1 · Cited by 54 · see citing works
PARP1 Stabilizes CTCF Binding and Chromatin Structure To Maintain Epstein-Barr Virus Latency Type
Lupey-Green, L. N., Caruso, L. B., Madžo, J., Martin, K. A., Tan, Y., Hulse, M., & Tempera, I. (2018) · Journal of Virology · doi:10.1128/jvi.00755-18 · Cited by 55 · see citing works
“PARP1 widely colocalizes with CTCF in human cell lines, and PARylation of CTCF modulates its insulator activity (15, 20, 21).”
BackgroundThe Role of Insulation in Patterning Gene Expression
Özdemir, I., & Gambetta, M. C. (2019) · Genes · doi:10.3390/genes10100767 · Cited by 43 · see citing works
Surviving an identity crisis: A revised view of chromatin insulators in the genomics era
Matzat, L. H., & Lei, E. P. (2013) · Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms · doi:10.1016/j.bbagrm.2013.10.007 · Cited by 44 · see citing works
In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila
Arzate-Mejía, R. G., Cerecedo-Castillo, A. J., Guerrero, G., Furlan-Magaril, M., & Recillas-Targa, F. (2019) · Nature Communications · doi:10.1038/s41467-020-14651-z · Cited by 36 · see citing works
Gene Positioning Effects on Expression in Eukaryotes
Nguyen, H. Q., & Bosco, G. (2015) · Annual Review of Genetics · doi:10.1146/annurev-genet-112414-055008 · Cited by 41 · see citing works
PARP1 restricts Epstein Barr Virus lytic reactivation by binding the BZLF1 promoter
Lupey-Green, L. N., Moquin, S. A., Martin, K. A., McDevitt, S., Hulse, M., Caruso, L. B., Pomerantz, R. T., Miranda, J. L., & Tempera, I. (2017) · Virology · doi:10.1016/j.virol.2017.04.006 · Cited by 43 · see citing works
“CTCF has emerged as an important cofactor of the host protein PARP1 (Farrar et al., 2011; Ong et al., 2013; Nalabothula et al., 2015; Zhao et al., 2015).”
BackgroundInfluentialNovel insights into PARPs in gene expression: regulation of RNA metabolism
Ke, Y., Zhang, J., Lv, X., Zeng, X., & Ba, X. (2019) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-019-03120-6 · Cited by 43 · see citing works
“For example, in Drosophila, PARylation of CTCF-interacting protein CP190 promotes its interaction with insulator DNA-binding proteins, suggesting that PARylation regulates the ability of insulators to mediate contacts between distant sites in the genome [39, 40].”
BackgroundChromatin binding of Gcn5 in Drosophila is largely mediated by CP190
Ali, T., Krüger, M., Bhuju, S., Jarek, M., Bartkuhn, M., & Renkawitz, R. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw1178 · Cited by 37 · see citing works
“Nuclei were isolated and the immunoprecipitation was carried out as previously described (34).”
MethodDopamine Triggers CTCF-Dependent Morphological and Genomic Remodeling of Astrocytes
Galloway, A. L., Adeluyi, A., O’Donovan, B., Fisher, M. L., Rao, C. N., Critchfield, P., Sajish, M., Turner, J. R., & Ortinski, P. I. (2018) · Journal of Neuroscience · doi:10.1523/jneurosci.3349-17.2018 · Cited by 36 · see citing works
Gibson, T. J., Larson, E. D., & Harrison, M. M. (2024) · Nature Structural & Molecular Biology · doi:10.1038/s41594-024-01231-8 · Cited by 30 · see citing works
“5a,c,e) 43–55 .”
RNA polymerases reshape chromatin architecture and couple transcription on individual fibers
Tullius, T. W., Isaac, R. S., Dubocanin, D., Ranchalis, J., Churchman, L. S., & Stergachis, A. B. (2024) · Molecular Cell · doi:10.1016/j.molcel.2024.08.013 · Cited by 31 · see citing works
Ideraabdullah, F., Thorvaldsen, J. L., Myers, J. A., & Bartolomei, M. S. (2014) · Human Molecular Genetics · doi:10.1093/hmg/ddu344 · Cited by 29 · see citing works
Many facades of CTCF unified by its coding for three-dimensional genome architecture
Wu, Q., Liu, P., & Wang, L. (2020) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2020.06.008 · Cited by 26 · see citing works
Insights into HP1a-Chromatin Interactions
Meyer‐Nava, S., Nieto-Caballero, V. E., Zurita, M., & Valadéz-Graham, V. (2020) · Cells · doi:10.3390/cells9081866 · Cited by 24 · see citing works
Identification of ADP-ribosylated peptides and ADP-ribose acceptor sites
Rosenthal, F. (2014) · Frontiers in bioscience · doi:10.2741/4266 · Cited by 26 · see citing works
“In addition to its role in the cellular stress response (12, 13), ADP-ribosylation regulates important cellular and biological processes such as replication, transcription, chromatin architecture, telomere maintenance, cell death, immune responses, and proteasomal functions (4, 14-22).”
BackgroundSchoborg, T. A., & Labrador, M. (2014) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-014-1672-6 · Cited by 21 · see citing works
“insulator function, clearly other factors are acting on insulators in parallel to orchestrate the complex organization of the chromatin fiber dictated by these elements [180].”
InfluentialRegulation of CTCF loop formation during pancreatic cell differentiation
Lyu, X., Rowley, M. J., Kulik, M. J., Dalton, S., & Corces, V. G. (2023) · Nature Communications · doi:10.1038/s41467-023-41964-6 · Cited by 21 · see citing works
“Several mechanisms have been suggested to regulate CTCF binding to DNA, including covalent modi fi cations of CTCF, DNA methylation and the recruitment of the ChAHP complex 37,55,29,56,57 .”
Plevock, K. M., Galletta, B. J., Slep, K. C., & Rusan, N. M. (2015) · PLoS ONE · doi:10.1371/journal.pone.0144174 · Cited by 23 · see citing works
Chromatin Insulators and Topological Domains: Adding New Dimensions to 3D Genome Architecture
Matharu, N. K., & Ahanger, S. H. (2015) · Genes · doi:10.3390/genes6030790 · Cited by 20 · see citing works
Glenn, S. E., & Geyer, P. (2018) · G3 Genes Genomes Genetics · doi:10.1534/g3.118.200705 · Cited by 20 · see citing works
Rao, V. K., Adusumalli, S., Tham, G.-H., Lin, W., Han, B., Benoukraf, T., Xu, G., & Ong, C. (2019) · Nucleic Acids Research · doi:10.1093/nar/gkz1144 · Cited by 20 · see citing works
“Site-directed mutagenesis was carried out as described previously (38) to generate wild-type and phosphor-mutant Tet3.”
MethodHaspin kinase modulates nuclear architecture and Polycomb-dependent gene silencing
Fresán, U., Rodríguez‐Sánchez, M. A., Reina, Ó., Corces, V. G., & Espinás, M. L. (2020) · PLoS Genetics · doi:10.1371/journal.pgen.1008962 · Cited by 19 · see citing works
“Insulator protein CP190 and Polycomb group of proteins were also found mostly associated with the nuclear matrix (S6A Fig), consistent with previously reported results [31,32].”
ResultChromatin Domain Organization of the TCRb Locus and Its Perturbation by Ectopic CTCF Binding
Rawat, P., Jalan, M., Sadhu, A., Kanaujia, A., & Srivastava, M. P. (2017) · Molecular and Cellular Biology · doi:10.1128/mcb.00557-16 · Cited by 15 · see citing works
“Poly(ADP-ribosyl)ation (PARylation) of CTCF was proposed to be critical to its insulator function in mammals (35) and its ability to act as a looping factor in Drosophila (36).”
BackgroundPoly(ADP-ribose) polymerase 1 in genome-wide expression control in Drosophila
Bordet, G., Lodhi, N., Guo, D., Kossenkov, A. V., & Tulin, A. V. (2020) · Scientific Reports · doi:10.1038/s41598-020-78116-5 · Cited by 13 · see citing works
The Drosophila speciation factor HMR localizes to genomic insulator sites
Gerland, T. A., Sun, B., Smialowski, P., Lukacs, A., Thomae, A. W., & Imhof, A. (2017) · PLoS ONE · doi:10.1371/journal.pone.0171798 · Cited by 10 · see citing works
Poly(ADP-ribosyl)ation of OVOL2 regulates aneuploidy and cell death in cancer cells
Zhang, R., Hong, J.-J., Yang, Q., Ong, C., Li, B., & Liou, Y. (2018) · Oncogene · doi:10.1038/s41388-018-0615-3 · Cited by 12 · see citing works
The chromatin factor ROW cooperates with BEAF‐32 in regulating long‐range inducible genes
Herman, N., Kadener, S., & Shifman, S. (2022) · EMBO Reports · doi:10.15252/embr.202254720 · Cited by 10 · see citing works
“Data were down-loaded from GSE40646 (Fuda et al , 2015; GAF), GSE116883 (Nazer et al , 2018; NELF-E), and GSE41354 (Ong et al , 2013; CP190).”
Moniot-Perron, L., Moindrot, B., Manceau, L., Edouard, J., Jaszczyszyn, Y., Gilardi‐Hebenstreit, P., Hernandez, C., Bloyer, S., & Noordermeer, D. (2023) · Cell Reports · doi:10.1016/j.celrep.2022.111967 · Cited by 9 · see citing works
Henderson, D., Miranda, J. L., & Emerson, B. M. (2017) · Oncotarget · doi:10.18632/oncotarget.19841 · Cited by 8 · see citing works
“Of particular interest is PARylation of the multi-functional DNA binding protein CTCF, which regulates its activity at chromatin boundaries and genetic insulators as well as epigenetic imprinting [9, 10].”
BackgroundAltered stability of nuclear lamin-B marks the onset of aging in male Drosophila
Lin, W., Ngian, Z., Koh, T., & Ong, C. (2022) · PLoS ONE · doi:10.1371/journal.pone.0265223 · Cited by 8 · see citing works
“Given that lamin-B and CP190 regulate the formation of LADs at the nuclear periphery and topological associating domains of the chromosomes [5, 11], changes in their solubility are likely to impact genome reorganization and gene expression in Day 30 male flies.”
BackgroundDrosophila CP190- and dCTCF-mediated enhancer blocking is augmented by SUMOylation
Jox, T., Buxa, M. K., Bohla, D., Ullah, I., Mačinković, I., Brehm, A., Bartkuhn, M., & Renkawitz, R. (2017) · Epigenetics & Chromatin · doi:10.1186/s13072-017-0140-6 · Cited by 7 · see citing works
“Therefore, we compared ChIP-Seq data of those proteins, which were already available at public data bases [18, 19, 41, 42].”
BackgroundMethodInfluentialMaintenance of a Drosophila melanogaster Population Cage
Caravaca, J. M., & Lei, E. P. (2016) · Journal of Visualized Experiments · doi:10.3791/53756 · Cited by 7 · see citing works
Gong, S., Hu, G., Guo, R., Zhang, J., Yang, Y., Ji, B., Li, G., & Yao, H. (2022) · Cell Regeneration · doi:10.1186/s13619-022-00131-w · Cited by 5 · see citing works
TADs Pair Homologous Chromosomes to Promote Interchromosomal Gene RegulationPreprint
Viets, K., Sauria, M. E. G., Chernoff, C., Anderson, C., Tran, S., Dove, A., Goyal, R., Voortman, L., Gordus, A. G., Taylor, J., et al. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/445627 · Cited by 6 · see citing works
“Transgenes were evaluated for insulator binding sites, Polycomb Group Complex binding sites, and the presence of chromatin marks using publicly available ChIP-chip and ChIP-seq datasets(Cuartero et al., 2014; Negre et al., 2010; Ong et al., 2013; Van Bortle et al., 2012; Wood et al., 2011).”
MethodTonelli, A., Cousin, P., Jankowski, A., Wang, B., Dorier, J., Barraud, J. F., Zunjarrao, S. S., & Gambetta, M. C. (2024) · Developmental Cell · doi:10.1016/j.devcel.2024.10.017 · Cited by 5 · see citing works
“Stronger WT insulators tended to have higher CTCF and Cp190 ChIP occupancy at their endogenous loci in S2 cells 47 (Figures S4E and S4F).”
InfluentialGodlewski, A., & Dziaman, T. (2026) · Frontiers in Aging · doi:10.3389/fragi.2026.1872180 · Cited by 4 · see citing works
“At the same time, interactions among PARP1, DNMT1, and CTCF demonstrate how metabolic state modulate regional DNA methylation patterns and genome architecture (Ummarino et al., 2021; Morales et al., 2014; Hassa, 2009; Zampieri et al., 2012; Ong et al., 2013).”
RNA polymerases reshape chromatin and coordinate transcription on individual fibersPreprint
Tullius, T. W., Isaac, R. S., Ranchalis, J., Dubocanin, D., Churchman, L. S., & Stergachis, A. B. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.12.22.573133 · Cited by 3 · see citing works
For Genomes to Stay in Shape, Insulators Must Be up to PAR
Wijchers, P. J., & de Laat, W. L. (2013) · Cell · doi:10.1016/j.cell.2013.09.012 · Cited by 1 · see citing works
Ishak, L., Moretton, A., Garreau-Balandier, I., Lefebvre, M., Alziari, S., Lachaume, P., Morel, F., Farge, G., Vernet, P., & Dubessay, P. (2016) · DNA repair · doi:10.1016/j.dnarep.2016.10.002 · Cited by 1 · see citing works
CDK5-mediated phosphorylation of CP190 may regulate locomotor activity in adult female Drosophila
Ong, C., & Lin, W. (2017) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2017.09.012 · Cited by 1 · see citing works
The chromatin factor ROW cooperates with BEAF-32 in regulating long-range inducible genesPreprint
Herman, N., Kadener, S., & Shifman, S. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.03.08.434270 · Cited by 1 · see citing works
Dynamics of higher order chromatin structures
Chen, X. (2014) · Figshare
Chromosome Conformation Capture on Chip (4C): Data Processing
Leblanc, B., Comet, I. V., Bantignies, F., & Cavalli, G. (2016) · Methods in molecular biology · doi:10.1007/978-1-4939-6380-5_21
In-Nucleus Hi-C in Drosophila Cells
Esquivel-López, A., Arzate‐Mejía, R. G., Pérez‐Molina, R., & Furlan-Magaril, M. (2021) · Journal of Visualized Experiments · doi:10.3791/62106
CTCF β-hydroxybutyrylation catalyzed by NAT10 regulates MHC-II gene expression
Zhou, R., Li, S., Jiang, S., Sui, Y., Ai, H., Su, R., Xiong, X., Qiu, H., Tong, X., & Zhao, W. (2026) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2026.106930
- Authors
- Ong, C.-T., Van Bortle, K., Ramos, E., & Corces, V. G.
- Affiliations
- Emory University (US)
- Corresponding author
- Corces, Victor G.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2013-09
- Volume
- 155
- Issue
- 1
- Pages
- 148-159
- ISSN
- 0092-8674 (ISSN-L)
- PMC
- PMC3816015
- OpenAlex
- W2080915753
- Cited by
- 70
- References
- 59
- Citations by year
- 2026: 2 · 2024: 3 · 2023: 3 · 2022: 5 · 2021: 3 · 2020: 7 · 2019: 4 · 2018: 5 · 2017: 7 · 2016: 12 · 2015: 5 · 2014: 10 · 2013: 4
- FWCI
- 4.63
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- U.S. Public Health Service
- National Institute of General Medical Sciences (R01GM035463)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Keywords
- poly(ADP-ribosyl)ation, insulators, CP190, poly(ADP-ribose) polymerase, Su(Hw), 3D genome organization, nuclear lamina, protein-protein interaction, Drosophila, chromatin structure
- MeSH terms
- Animals, Cell Differentiation, DNA-Binding Proteins, Drosophila melanogaster, Mutation, Poly Adenosine Diphosphate Ribose, Poly(ADP-ribose) Polymerases, Nuclear Matrix, Drosophila Proteins, Insulator Elements, Chromosomes, Insect, Polytene Chromosomes
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