The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Generation of Functional Human Pancreatic β Cells In Vitro
Open accessAbstract
The generation of insulin-producing pancreatic β cells from stem cells in vitro would provide an unprecedented cell source for drug discovery and cell transplantation therapy in diabetes. However, insulin-producing cells previously generated from human pluripotent stem cells (hPSC) lack many functional characteristics of bona fide β cells. Here, we report a scalable differentiation protocol that can generate hundreds of millions of glucose-responsive β cells from hPSC in vitro. These stem-cell-derived β cells (SC-β) express markers found in mature β cells, flux Ca(2+) in response to glucose, package insulin into secretory granules, and secrete quantities of insulin comparable to adult β cells in response to multiple sequential glucose challenges in vitro. Furthermore, these cells secrete human insulin into the serum of mice shortly after transplantation in a glucose-regulated manner, and transplantation of these cells ameliorates hyperglycemia in diabetic mice.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Transplantation of SC-islets into mice improves their insulin secretion and glucose responsiveness, suggesting that SC-islet immaturity can be resolved.”
From Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes (2023) · cites this paper as [36]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“and the largest SC-islet differentiation that has been reported in literature was performed in 500 mL magnetic spinner flasks.36”
From Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes (2023) · cites this paper as [36]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“These stem cells begin as proliferative undifferentiated cells that are exposed to a series of cycles of growth and differentiation factors that lead to seven defined stages.”
From The future of type 1 diabetes therapy (2025) · cites this paper as [68]
Experimental — accuracy not yet measuredThe cited source says what the claim attributes to it.
In the citing paper
“In 2014-15, several groups published protocols for generating stem cell-derived β (SC-β) cells that secreted insulin in response to glucose stimulation.36–38”
From Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes (2023) · cites this paper as [36]
The cited source says what the claim attributes to it.
In the citing paper
“These cells have been shown to improve glycemic control in diabetic mice, highlighting their potential utility in vivo.36,37,39–44”
From Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes (2023) · cites this paper as [36]
The cited source says what the claim attributes to it.
In the citing paper
“SC-islet protocols that are performed completely in suspension culture36 benefit from the natural three-dimensional scaling of these systems.”
From Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes (2023) · cites this paper as [36]
The cited source says what the claim attributes to it.
In the citing paper
“Methods have been developed (figure 2) by which pluripotent stem cells can be differentiated into βlike cells by a series of steps in which differentiation factors are added.68,69”
From The future of type 1 diabetes therapy (2025) · cites this paper as [68]
The check wasn’t confident either way, so it gives no verdict — never disputed.
In the citing paper
“The first four stages produce proliferative cells.As the cells differentiate, they become less proliferative.”
From The future of type 1 diabetes therapy (2025) · cites this paper as [68]
Experimental — accuracy not yet measuredIn the citing paper
“For instance, Pagliuca and colleagues kept human embryonic stem cells suspended in liquid culture at 70 rpm, added specific growth factors to encourage differentiation, and found that the resulting beta-like cells behaved similarly to mature beta cells220.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [220]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Takahashi, K., & Yamanaka, S. (2006) · Cell · doi:10.1016/j.cell.2006.07.024 · Cited by 26,673
Kriks, S., Shim, J., Piao, J., Ganat, Y. M., Wakeman, D. R., Xie, Z., Carrillo‐Reid, L., Auyeung, G., Antonacci, C. L., Buch, A., et al. (2011) · Nature · doi:10.1038/nature10648 · Cited by 1,951
International trial of the Edmonton protocol for islet transplantation
Shapiro, A. M. J., Ricordi, C., Hering, B. J., Auchincloss, H., Lindblad, R., Robertson, R. P., Secchi, A., Brendel, M. D., Berney, T., Brennan, D. C., et al. (2006) · New England Journal of Medicine · doi:10.1056/nejmoa061267 · Cited by 1,942
Production of pancreatic hormone–expressing endocrine cells from human embryonic stem cells
D’Amour, K. A., Bang, A. G., Eliazer, S., Kelly, O., Agulnick, A. D., Smart, N. G., Moorman, M. A., KROON, E. J., Carpenter, M. K., & Baetge, E. E. (2006) · Nature Biotechnology · doi:10.1038/nbt1259 · Cited by 1,913
Kroon, E., Martinson, L. A., Kadoya, K., Bang, A. G., Kelly, O. G., Eliazer, S., Young, H., Richardson, M., Smart, N. G., Cunningham, J., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt1393 · Cited by 1,843 · cited 5×
“Transplantation of pancreatic progenitors expressing PDX1+/ NKX6-1+ (PP2 in Figure 1A) into mice gives rise to functional b cells in vivo after 3–4 months (Kroon et al., 2008; Rezania et al., 2012).”
BackgroundResultInfluentialEfficient differentiation of human embryonic stem cells to definitive endoderm
D'Amour, K. A., Agulnick, A. D., Eliazer, S., Kelly, O. G., Kroon, E., & Baetge, E. E. (2005) · Nature Biotechnology · doi:10.1038/nbt1163 · Cited by 1,730
Keirstead, H. S., Nistor, G., Bernal, G. M., Totoiu, M. O., Cloutier, F., Sharp, K. L., & Steward, O. (2005) · Journal of Neuroscience · doi:10.1523/jneurosci.0311-05.2005 · Cited by 1,263
Notch signalling controls pancreatic cell differentiation
Apelqvist, Å., Li, H., Sommer, L., Beatus, P., Anderson, D. J., Honjo, T., de Angelis, M. H., Lendahl, U., & Edlund, H. (1999) · Nature · doi:10.1038/23716 · Cited by 1,158
Induction of Pancreatic Differentiation by Signals from Blood Vessels
Lammert, E., Cleaver, O. B., & Melton, D. A. (2001) · Science · doi:10.1126/science.1064344 · Cited by 1,064
Self-citationNotch signaling controls multiple steps of pancreatic differentiation
Murtaugh, L. C., Stanger, B. Z., Kwan, K. M., & Melton, D. A. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2436557100 · Cited by 782
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Items 301–400 of 1,004
Richardson, T., Barner, S., Candiello, J., Kumta, P. N., & Banerjee, I. (2016) · Acta Biomaterialia · doi:10.1016/j.actbio.2016.02.025 · Cited by 66
Pig-to-Primate Islet Xenotransplantation: Past, Present, and Future
Liu, Z., Hu, W., He, T., Dai, Y., Hara, H., Bottino, R., Cooper, D. K. C., Cai, Z., & Mou, L. (2017) · Cell Transplantation · doi:10.3727/096368917x694859 · Cited by 70
Weng, C., Xi, J., Li, H., Cui, J., Gu, A., Lai, S., Leskov, K., Ke, L., Jin, F., & Li, Y. (2020) · Nature Metabolism · doi:10.1038/s42255-020-00314-2 · Cited by 65
Mitigating Ischemic Injury of Stem Cell-Derived Insulin-Producing Cells after Transplant
Faleo, G., Russ, H. A., Wisel, S., Parent, A. V., Nguyen, V., Nair, G. G., Freise, J. E., Villanueva, K. E., Szot, G. L., Hebrok, M., et al. (2017) · Stem Cell Reports · doi:10.1016/j.stemcr.2017.07.012 · Cited by 65
Genetic and pharmacologic inhibition of ALDH1A3 as a treatment of β-cell failure
Son, J., Du, W. J., Esposito, M., Shariati, K., Ding, H., Kang, Y., & Accili, D. (2023) · Nature Communications · doi:10.1038/s41467-023-36315-4 · Cited by 67
Massey, J., Liu, Y., Alvarenga, O. E., Saez, T., Schmerer, M. W., & Warmflash, A. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1815363116 · Cited by 67
Tacrolimus- and sirolimus-induced human β cell dysfunction is reversible and preventable
Dai, C., Walker, J. T., Shostak, A., Padgett, A., Spears, E., Wisniewski, S., Poffenberger, G., Aramandla, R., Dean, E. D., Prasad, N., et al. (2020) · JCI Insight · doi:10.1172/jci.insight.130770 · Cited by 64
Self-citationA Chromatin Basis for Cell Lineage and Disease Risk in the Human Pancreas
Arda, H. E., Tsai, J., Rosli, Y., Giresi, P. G., Bottino, R., Greenleaf, W. J., Chang, H. Y., & Kim, S. K. (2018) · Cell Systems · doi:10.1016/j.cels.2018.07.007 · Cited by 62
Centroacinar cells: At the center of pancreas regeneration
Beer, R. L., Parsons, M. J., & Rovira, M. (2016) · Developmental Biology · doi:10.1016/j.ydbio.2016.02.027 · Cited by 73
Serotonergic regulation of insulin secretion
Cataldo, L. R., Lyons, C., Bennet, H., Artner, I., & Fex, M. (2018) · Acta Physiologica · doi:10.1111/apha.13101 · Cited by 67
Maturation of beta cells: lessons from in vivo and in vitro models
Barsby, T., & Otonkoski, T. (2022) · Diabetologia · doi:10.1007/s00125-022-05672-y · Cited by 68
Biodegradable and Non-Biodegradable Biomaterials and Their Effect on Cell Differentiation
Geevarghese, R., Sajjadi, S. S., Hudecki, A., Sajjadi, S., Jalal, N. R., Madrakian, T., Ahmadi, M., Włodarczyk‐Biegun, M. K., Ghavami, S., Likus, W., et al. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232416185 · Cited by 69
Graham, M. L., & Schuurman, H. (2015) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2015.02.054 · Cited by 66
Kim, J., Kim, H. W., Cha, K. J., Han, J., Jang, Y. J., Kim, J., & Kim, J. (2016) · ACS Nano · doi:10.1021/acsnano.5b06985 · Cited by 63
Cell Therapy for Type 1 Diabetes: Current and Future Strategies
Aghazadeh, Y., & Nostro, M. C. (2017) · Current Diabetes Reports · doi:10.1007/s11892-017-0863-6 · Cited by 66
Stem Cell Therapies for Reversing Vision Loss
Higuchi, A., Kumar, S. S., Benelli, G., Alarfaj, A. A., Munusamy, M. A., Umezawa, A., & Murugan, K. (2017) · Trends in biotechnology · doi:10.1016/j.tibtech.2017.06.016 · Cited by 63
Effect of polysaccharides from adlay seed on anti-diabetic and gut microbiota
Chen, L.-C., Fan, Z.-Y., Wang, H.-Y., Wen, D.-C., & Zhang, S. (2019) · Food & Function · doi:10.1039/c9fo00406h · Cited by 63
Aghazadeh, Y., Sarangi, F., Poon, F., Nkennor, B., McGaugh, E. C., Nunes, S. S., & Nostro, M. C. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2022.03.004 · Cited by 62
Advances in β cell replacement and regeneration strategies for treating diabetes
Benthuysen, J. R., Carrano, A. C., & Sander, M. (2016) · Journal of Clinical Investigation · doi:10.1172/jci87439 · Cited by 68
Gerace, D., Martiniello‐Wilks, R., Nassif, N. T., Lal, S., Steptoe, R. J., & Simpson, A. M. (2017) · Stem Cell Research & Therapy · doi:10.1186/s13287-017-0511-8 · Cited by 62
Pesaresi, M., Sebastián-Pérez, R., & Cosma, M. P. (2018) · FEBS Journal · doi:10.1111/febs.14633 · Cited by 63
Protecting Stem Cell Derived Pancreatic Beta-Like Cells From Diabetogenic T Cell Recognition
Castro-Gutiérrez, R., Alkanani, A. K., Mathews, C. E., Michels, A. W., & Russ, H. A. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.707881 · Cited by 60
Conrad, E., Dai, C., Spaeth, J. M., Guo, M. A., Cyphert, H. A., Scoville, D. W., Carroll, J. M., Yu, W.-M., Goodrich, L. V., Harlan, D. M., et al. (2015) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00285.2015 · Cited by 62
Ramond, C., Glaser, N., Berthault, C., Ameri, J., Kirkegaard, J. S., Hansson, M., Honoré, C., Semb, H., & Scharfmann, R. (2017) · eLife · doi:10.7554/elife.27564 · Cited by 54
Loss of MANF Causes Childhood-Onset Syndromic Diabetes Due to Increased Endoplasmic Reticulum Stress
Montaser, H., Patel, K., Balboa, D., Ibrahim, H., Lithovius, V., Näätänen, A., Chandra, V., Demir, K., Acar, S., Ben‐Omran, T., et al. (2021) · Diabetes · doi:10.2337/db20-1174 · Cited by 63
Human pluripotent stem cell-derived insulin-producing cells: A regenerative medicine perspective
Migliorini, A., Nostro, M. C., & Sneddon, J. B. (2021) · Cell Metabolism · doi:10.1016/j.cmet.2021.03.021 · Cited by 62
Liang, Z., Sun, D., Lu, S., Lei, Z., Wang, S., Luo, Z., Zhan, J., Wu, S., Jiang, Y., Lu, Z., et al. (2023) · Nature Metabolism · doi:10.1038/s42255-022-00713-7 · Cited by 59
Nadeau, L., Patten, D. A., Caron, A., Garneau, L., Pinault-Masson, É., Foretz, M., Haddad, P., Anderson, B. G., Quinn, L. S., Jardine, K. E., et al. (2018) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2018.10.021 · Cited by 62
From Mesenchymal Stromal/Stem Cells to Insulin-Producing Cells: Progress and Challenges
Ghoneim, M. A., Refaie, A. F., Elbassiouny, B. L., Gabr, M. M., & Zakaria, M. M. (2020) · Stem Cell Reviews and Reports · doi:10.1007/s12015-020-10036-3 · Cited by 62
López-Méndez, T. B., Santos‐Vizcaíno, E., Pedraz, J. L., Orive, G., & Hernández, R. M. (2021) · Journal of Controlled Release · doi:10.1016/j.jconrel.2021.06.006 · Cited by 61
Dysfunctions, molecular mechanisms, and therapeutic strategies of pancreatic β-cells in diabetes
Prasad, M. K., Mohandas, S., & Ramkumar, K. M. (2023) · APOPTOSIS · doi:10.1007/s10495-023-01854-0 · Cited by 61
The bioartificial pancreas (BAP): Biological, chemical and engineering challenges
Iacovacci, V., Ricotti, L., Menciassi, A., & Dario, P. (2015) · Biochemical Pharmacology · doi:10.1016/j.bcp.2015.08.107 · Cited by 68
Bioprinting and Cellular Therapies for Type 1 Diabetes
Ravnic, D. J., Leberfinger, A. N., & Ozbolat, I. T. (2017) · Trends in biotechnology · doi:10.1016/j.tibtech.2017.07.006 · Cited by 59
Manzoli, V., Villa, C., Bayer, A. L., Morales, L. C., Molano, R. D., Torrente, Y., Ricordi, C., Hubbell, J. A., & Tomei, A. A. (2017) · American Journal of Transplantation · doi:10.1111/ajt.14547 · Cited by 60
Kim, J., Park, K., Kim, M. J., Lim, H. J., Kim, K. H., Kim, S., Lee, E., Kim, S., Kim, S. J., Hur, K. Y., et al. (2021) · Nature Communications · doi:10.1038/s41467-020-20454-z · Cited by 62
The Importance of Proper Oxygenation in 3D Culture
Tse, H., Gardner, G., Domínguez-Bendala, J., & Fraker, C. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.634403 · Cited by 54
Extracellular Matrix and Integrins in Embryonic Stem Cell Differentiation
Wang, H., Luo, X., & Leighton, J. (2015) · Biochemistry Insights · doi:10.4137/bci.s30377 · Cited by 59
Biomaterials used in stem cell therapy for spinal cord injury
Higuchi, A., Kumar, S. S., Benelli, G., Ling, Q., Li, H.-F., Alarfaj, A. A., Munusamy, M. A., Sung, T., Chang, Y., & Murugan, K. (2019) · Progress in Materials Science · doi:10.1016/j.pmatsci.2019.02.002 · Cited by 59
Bi, H., Karanth, S. S., Ye, K., Stein, R., & Jin, S. (2020) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.0c00088 · Cited by 56
Chemical compound-based direct reprogramming for future clinical applications.
Takeda, Y., Harada, Y., Yoshikawa, T., & Dai, P. (2018) · Bioscience Reports · doi:10.1042/bsr20171650 · Cited by 57
Preparation, characterization and anti-diabetic activity of polysaccharides from adlay seed
Chen, L.-C., Jiang, B.-K., Zheng, W., Zhang, S., Li, J.-J., & Fan, Z.-Y. (2019) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2019.08.018 · Cited by 58
Differentiating functional human islet-like aggregates from pluripotent stem cells
Barsby, T., Ibrahim, H., Lithovius, V., Montaser, H., Balboa, D., Vähäkangas, E., Chandra, V., Saarimäki‐Vire, J., & Otonkoski, T. (2022) · STAR Protocols · doi:10.1016/j.xpro.2022.101711 · Cited by 58
β-cell neogenesis: A rising star to rescue diabetes mellitus
Niu, F., Liu, W., Ren, Y., Tian, Y., Shi, W., Li, M., Li, Y., Xiong, Y., & Lü, Q. (2023) · Journal of Advanced Research · doi:10.1016/j.jare.2023.10.008 · Cited by 68
Alsereidi, F. R., Khashim, Z., Marzook, H., Al-Rawi, A. M., Salomon, T., Almansoori, M. K., Madkour, M. M., Hamam, A. M., Ramadan, M. M., Peterson, Q. P., et al. (2024) · Cardiovascular Diabetology · doi:10.1186/s12933-024-02481-y · Cited by 60
Self-citationPancreatic islet cell development and regeneration
Romer, A. I., & Sussel, L. (2015) · Current Opinion in Endocrinology Diabetes and Obesity · doi:10.1097/med.0000000000000174 · Cited by 57
Davenport, C., Diekmann, U., Budde, I., Detering, N. T., & Naujok, O. (2016) · Stem Cells · doi:10.1002/stem.2428 · Cited by 56
Pathways governing development of stem cell‐derived pancreatic β cells: lessons from embryogenesis
Al‐Khawaga, S., Memon, B., Butler, A. E., Taheri, S., Abou‐Samra, A., & Abdelalim, E. M. (2017) · Biological reviews/Biological reviews of the Cambridge Philosophical Society · doi:10.1111/brv.12349 · Cited by 59
Wang, X., Brown, N. K., Wang, B., Shariati, K., Wang, K., Fuchs, S., Melero‐Martin, J. M., & Ma, M. (2021) · Advanced Science · doi:10.1002/advs.202003708 · Cited by 57
Yang, K., O’Cearbhaill, E. D., Liu, S. S., Zhou, A., Chitnis, G. D., Hamilos, A. E., Xu, J., Verma, M. K. S., Giraldo, J. A., Kudo, Y., et al. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2101258118 · Cited by 58
“In the SC-β C protocol, SC-β Cs grow in 3D clusters, of which ∼ 30% are β cells that have the ability to secrete insulin in response to glucose (11).”
InfluentialSelf-citationDifferential Function and Maturation of Human Stem Cell-Derived Islets After Transplantation
Maxwell, K. G., Kim, M. H., Gale, S. E., & Millman, J. R. (2022) · Stem Cells Translational Medicine · doi:10.1093/stcltm/szab013 · Cited by 54
Self-citationAbnormal exocrine–endocrine cell cross-talk promotes β-cell dysfunction and loss in MODY8
Kahraman, S., Dirice, E., Basile, G., Diegisser, D., Alam, J., Johansson, B. B., Gupta, M. K., Hu, J., Huang, L., Soh, C.-L., et al. (2022) · Nature Metabolism · doi:10.1038/s42255-021-00516-2 · Cited by 53
Pancreatic islet transplantation: current advances and challenges
Wang, Q., Huang, Y., Liu, L., Zhao, X., Sun, Y., Mao, X., & Li, S.-W. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1391504 · Cited by 63
Raikwar, S. P., Kim, E. M., Sivitz, W. I., Allamargot, C., Thedens, D. R., & Zavazava, N. (2015) · PLoS ONE · doi:10.1371/journal.pone.0116582 · Cited by 59
Chemical Modulation of Cell Fate in Stem Cell Therapeutics and Regenerative Medicine
Liu, K., Chen, Y., Xie, M., Li, K., & Ding, S. (2016) · Cell chemical biology · doi:10.1016/j.chembiol.2016.07.007 · Cited by 60
Higuchi, A., Kumar, S. S., Ling, Q., Alarfaj, A. A., Munusamy, M. A., Murugan, K., Hsu, S.-T., Benelli, G., & Umezawa, A. (2016) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2016.09.002 · Cited by 57
Zebrafish Pancreas Development and Regeneration
Prince, V., Anderson, R. M., & Dalgin, G. (2016) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/bs.ctdb.2016.10.005 · Cited by 64
Concise Review: Human Pluripotent Stem Cells for the Modeling of Pancreatic β‐Cell Pathology
Balboa, D., Saarimäki-Vire, J., & Otonkoski, T. (2018) · Stem Cells · doi:10.1002/stem.2913 · Cited by 48
Gentsch, G. E., Spruce, T., Owens, N. D. L., & Smith, J. C. (2019) · Nature Communications · doi:10.1038/s41467-019-12263-w · Cited by 56
Johnson, J. D. (2016) · Diabetologia · doi:10.1007/s00125-016-4059-4 · Cited by 56
TGF-β family ligands exhibit distinct signalling dynamics that are driven by receptor localisation
Miller, D. S. J., Schmierer, B., & Hill, C. S. (2019) · Journal of Cell Science · doi:10.1242/jcs.234039 · Cited by 56
Back and forth in time: Directing age in iPSC-derived lineages
Cornacchia, D., & Studer, L. (2017) · Brain Research · doi:10.1016/j.brainres.2015.11.013 · Cited by 56
Foo, K. S., Lehtinen, M. L., Leung, C. Y., Lian, X. L., Xu, J., Keung, W., Geng, L., Kolstad, T. R., Thams, S., Wong, A. O.-T., et al. (2018) · Molecular Therapy · doi:10.1016/j.ymthe.2018.02.012 · Cited by 58
Hope vs hype: where are we in type 1 diabetes?
Skyler, J. S. (2018) · Diabetologia · doi:10.1007/s00125-017-4530-x · Cited by 51
Human expandable pancreatic progenitor–derived β cells ameliorate diabetes
Ma, X., Lu, Y., Zhou, Z., Li, Q., Chen, X., Wang, W., Jin, Y., Hu, Z., Chen, G., Deng, Q., et al. (2022) · Science Advances · doi:10.1126/sciadv.abk1826 · Cited by 60
Species-specific developmental timing is maintained by pluripotent stem cells ex utero
Barry, C., Schmitz, M. T., Jiang, P., Schwartz, M. P., Duffin, B. M., Swanson, S. A., Bacher, R. L., Bolin, J. M., Elwell, A. L., McIntosh, B. E., et al. (2017) · Developmental Biology · doi:10.1016/j.ydbio.2017.02.002 · Cited by 53
Probing the missing mature β-cell proteomic landscape in differentiating patient iPSC-derived cells
Vethe, H., Bjørlykke, Y., Ghila, L., Paulo, J. A., Scholz, H., Gygi, S. P., Chera, S., & Ræder, H. (2017) · Scientific Reports · doi:10.1038/s41598-017-04979-w · Cited by 53
Whole Organ Engineering: Approaches, Challenges, and Future Directions
Sohn, S., Buskirk, M. V., Buckenmeyer, M. J., Londoño, R., & Faulk, D. M. (2020) · Applied Sciences · doi:10.3390/app10124277 · Cited by 57
Islet organoid as a promising model for diabetes
Zhang, X., Ma, Z., Song, E., & Xu, T. (2021) · Protein & Cell · doi:10.1007/s13238-021-00831-0 · Cited by 58
Functional genomics and the future of iPSCs in disease modeling
Brooks, I. R., Garrone, C. M., Kerins, C., Kiar, C. S., Syntaka, S., Xu, J. Z., Spagnoli, F. M., & Watt, F. M. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2022.03.019 · Cited by 54
Human Beta Cell Regenerative Drug Therapy for Diabetes: Past Achievements and Future Challenges
Wang, P., Karaköse, E., Choleva, L., Kumar, K., DeVita, R. J., García-Ocaña, A., & Stewart, A. F. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.671946 · Cited by 54
Zhao, Y. L., Jiang, Z., Delgado, E., Li, H., Zhou, H., Hu, W., Pérez‐Basterrechea, M., Janošťáková, A., Tan, Q., Wang, J., et al. (2017) · Stem Cells Translational Medicine · doi:10.1002/sctm.17-0078 · Cited by 54
Ng, N. H. J., Jasmen, J. B., Lim, C. S., Lau, H. H., Krishnan, V. G., Kadiwala, J., Kulkarni, R. N., Ræder, H., Vallier, L., Hoon, S., et al. (2019) · iScience · doi:10.1016/j.isci.2019.05.032 · Cited by 54
Hossain, M. K., Dayem, A. A., Han, J., Saha, S. K., Yang, G., Choi, H. Y., & Cho, S. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17020256 · Cited by 53
Emerging diabetes therapies: Bringing back the β-cells
Basile, G., Qadir, M. M. F., Mauvais‐Jarvis, F., Vetere, A., Shoba, V. M., Modell, A. E., Pastori, R. L., Russ, H. A., Wagner, B. K., & Domínguez‐Bendala, J. (2022) · Molecular Metabolism · doi:10.1016/j.molmet.2022.101477 · Cited by 56
Advancing Organoid Engineering for Tissue Regeneration and Biofunctional Reconstruction
Jin, H., Xue, Z., Liu, J., Ma, B., Yang, J., & Lei, L. (2024) · Biomaterials Research · doi:10.34133/bmr.0016 · Cited by 58
Bonfanti, P., Nobécourt, E., Oshima, M., Albagli, O., Laurysens, V., Stangé, G. M., Sojoodi, M., Heremans, Y., Heimberg, H., & Scharfmann, R. (2015) · Stem Cells and Development · doi:10.1089/scd.2014.0550 · Cited by 53
Pluripotent stem cell applications for regenerative medicine
Angelos, M. G., & Kaufman, D. S. (2015) · Current Opinion in Organ Transplantation · doi:10.1097/mot.0000000000000244 · Cited by 54
Current stem cell based therapies in diabetes.
Ma, L., Davis, M. F., Je, F., Eb, T., & Gallicano, G. I. (2016) · Europe PMC (PubMed Central) · Cited by 53
Wang, K., Wang, X., Han, C., Hou, W., Wang, J., Chen, L., & Luo, Y. (2016) · Advanced Materials · doi:10.1002/adma.201604600 · Cited by 52
Chen, Y., Chen, L.-H., Li, M.-P., Li, H.-F., Higuchi, A., Kumar, S. S., Ling, Q., Alarfaj, A. A., Munusamy, M. A., Chang, Y., et al. (2017) · Scientific Reports · doi:10.1038/srep45146 · Cited by 53
Zhao, M., Jiang, J., Zhao, M., Chang, C., Wu, H., & Lu, Q. (2020) · Clinical Reviews in Allergy & Immunology · doi:10.1007/s12016-020-08813-6 · Cited by 51
Pullen, L. C. (2018) · American Journal of Transplantation · doi:10.1111/ajt.14954 · Cited by 53
A Stem Cell Approach to Cure Type 1 DiabetesPreprint
Helman, A., & Melton, D. A. (2020) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a035741 · Cited by 56
Self-citationStomach-derived human insulin-secreting organoids restore glucose homeostasis
Huang, X., Gu, W. Y., Zhang, J., Lan, Y., Colarusso, J. L., Li, S., Pertl, C., Lü, J., Kim, H., Zhu, J., et al. (2023) · Nature Cell Biology · doi:10.1038/s41556-023-01130-y · Cited by 56
Continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surfaces
Peng, I.-C., Yeh, C.-C., Lu, Y., Muduli, S., Ling, Q., Alarfaj, A. A., Munusamy, M. A., Kumar, S. S., Murugan, K., Lee, H.-C., et al. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.10.039 · Cited by 52
Zhang, X., Cui, T., He, J., Wang, H., Cai, R., Popovics, P., Vidaurre, I., Sha, W., Schmid, J., Ludwig, B., et al. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1518540112 · Cited by 52
Castiello, F. R., Heileman, K., & Tabrizian, M. (2015) · Lab on a Chip · doi:10.1039/c5lc01046b · Cited by 61
IAPP and type 1 diabetes: implications for immunity, metabolism and islet transplants
Denroche, H. C., & Verchere, C. B. (2018) · Journal of Molecular Endocrinology · doi:10.1530/jme-17-0138 · Cited by 53
(Re)generating Human Beta Cells: Status, Pitfalls, and Perspectives
Baeyens, L., Lemper, M., Staels, W., Groef, S. D., Leu, N. D., Heremans, Y., German, M. S., & Heimberg, H. (2018) · Physiological Reviews · doi:10.1152/physrev.00034.2016 · Cited by 52
2020 vision – An overview of prospects for diabetes management and prevention in the next decade
Wang, C., Neil, D. L., & Home, P. (2018) · Diabetes Research and Clinical Practice · doi:10.1016/j.diabres.2018.06.007 · Cited by 51
Manaph, N. P. A., Sivanathan, K. N., Nitschke, J., Zhou, X., Coates, P. T. H., & Drogemuller, C. (2019) · Stem Cell Research & Therapy · doi:10.1186/s13287-019-1396-5 · Cited by 52
Differential roles of FOXO transcription factors on insulin action in brown and white adipose tissue
Homan, E. P., Brandão, B. B., Softic, S., Ouaamari, A. E., O'Neill, B. T., Kulkarni, R. N., Kim, J. K., & Kahn, C. R. (2021) · Journal of Clinical Investigation · doi:10.1172/jci143328 · Cited by 51
Gabr, M. M., Zakaria, M. M., Refaie, A. F., Abdel‐Rahman, E. A., Reda, A. M., Ali, S. S., Khater, S. M., Ashamallah, S. A., Ismail, A. M., Ismail, H. E.-D. A., et al. (2017) · BioMed Research International · doi:10.1155/2017/3854232 · Cited by 51
Youngblood, R. L., Sampson, J. P., Lebioda, K. R., Spicer, G., & Shea, L. D. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.06.032 · Cited by 57
Augsornworawat, P., Velazco-Cruz, L., Song, J., & Millman, J. R. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.08.031 · Cited by 51
Self-citationTargeting the receptor for advanced glycation end products (RAGE) in type 1 diabetes
Bagge, S. L., Fotheringham, A. K., Leung, S. S., & Forbes, J. M. (2020) · Medicinal Research Reviews · doi:10.1002/med.21654 · Cited by 54
Liu, W. H., Flanders, J. A., Wang, L., Liu, Q., Bowers, D. T., Wang, K., Chiu, A., Wang, X., Ernst, A. U., Shariati, K., et al. (2021) · Small · doi:10.1002/smll.202104899 · Cited by 54
Epigenetic modifications and long noncoding RNAs influence pancreas development and function
Arnes, L., & Sussel, L. (2015) · Trends in Genetics · doi:10.1016/j.tig.2015.02.008 · Cited by 54
Sabek, O. M., Farina, M., Fraga, D. W., Afshar, S. F., Ballerini, A., Filgueira, C. S., Thekkedath, U. R., Grattoni, A., & Gaber, A. O. (2016) · Journal of Tissue Engineering · doi:10.1177/2041731416638198 · Cited by 51
Advances in Cell Replacement Therapies for Diabetes
Hering, B. J., Rickels, M. R., Bellin, M. D., Millman, J. R., Tomei, A. A., Garcı́a, A. J., Shirwan, H., Stabler, C. L., Ma, M., Peng, Y., et al. (2025) · Diabetes · doi:10.2337/db25-0037 · Cited by 55
Self-citation
Metadata
- Authors
- Pagliuca, F. W., Millman, J. R., Gürtler, M., Segel, M., Van Dervort, A., Ryu, J. H., Peterson, Q. P., Greiner, D., & Melton, D. A.
- Affiliations
- Harvard University (US)
- University of Massachusetts Chan Medical School (US)
- Corresponding author
- Melton, Douglas A.
- ORCID
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2014-10
- Volume
- 159
- Issue
- 2
- Pages
- 428-439
- ISSN
- 0092-8674 (ISSN-L)
- PMC
- PMC4617632
- OpenAlex
- W2148890861
- Cited by
- 2,025
- References
- 50
- Citations by year
- 2026: 104 · 2025: 147 · 2024: 152 · 2023: 159 · 2022: 183 · 2021: 196 · 2020: 185 · 2019: 168 · 2018: 175 · 2017: 181 · 2016: 200 · 2015: 157 · 2014: 16
- FWCI
- 183.24
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Howard Hughes Medical Institute
- National Institutes of Health (1uc4dk104218-01)
- Harvard Stem Cell Institute
- National Institute of Diabetes and Digestive and Kidney Diseases (UC4-DK-104218, U01DK089572, P30DK020593)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Keywords
- pancreatic beta cells, human pluripotent stem cells, stem cell-derived beta cells, glucose-dependent insulin secretion, beta cell differentiation, insulin secretion, calcium flux, cell transplantation, diabetes mellitus, hyperglycemia, secretory granules
- MeSH terms
- Animals, Humans, Insulin, Islets of Langerhans, Cell Culture Techniques, Pluripotent Stem Cells, Insulin-Secreting Cells, Mice, Induced Pluripotent Stem Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
