The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Quantifying Memory CD8 T Cells Reveals Regionalization of Immunosurveillance
Open accessAbstract
Memory CD8 T cells protect against intracellular pathogens by scanning host cell surfaces; thus, infection detection rates depend on memory cell number and distribution. Population analyses rely on cell isolation from whole organs, and interpretation is predicated on presumptions of near complete cell recovery. Paradigmatically, memory is parsed into central, effector, and resident subsets, ostensibly defined by immunosurveillance patterns but in practice identified by phenotypic markers. Because isolation methods ultimately inform models of memory T cell differentiation, protection, and vaccine translation, we tested their validity via parabiosis and quantitative immunofluorescence microscopy of a mouse memory CD8 T cell population. We report three major findings: lymphocyte isolation fails to recover most cells and biases against certain subsets, residents greatly outnumber recirculating cells within non-lymphoid tissues, and memory subset homing to inflammation does not conform to previously hypothesized migration patterns. These results indicate that most host cells are surveyed for reinfection by segregated residents rather than by recirculating cells that migrate throughout the blood and body.
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“Through parabiosis experiments, it has been shown that both CD69+ and CD69− memory T cells of non-lymphoid tissues, like kidney and liver, are tissue resident [239].”
From Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) (2021) · cites this paper as [239]
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“While the majority of Trm cells throughout different tissues constitutively express CD69, parabiosis, and in vivo labeling experiments have highlighted that there are CD69− cells that reside long-term in tissues [239].”
From Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) (2021) · cites this paper as [239]
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“They have been defined as independent populations by tissue retention in parabiosis experiments [239, 240], by distinguishing transcriptional profiles [230, 241-245], by exclusive Ag receptor repertoires and specificities [243, 244, 246], and by exclusive persistence in transplanted tissues [247, 248].”
From Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) (2021) · cites this paper as [239]
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Cites
Two subsets of memory T lymphocytes with distinct homing potentials and effector functions
Sallusto, F., Lenig, D., Förster, R., Lipp, M., & Lanzavecchia, A. (1999) · Nature · doi:10.1038/44385 · Cited by 5,846 · cited 2×
“Paradigmatically, TEM recirculate through NLT or respond to NLT sites of inflammation, while TCM limit recirculation to SLO (Sallusto et al., 1999).”
BackgroundPhenotypic Analysis of Antigen-Specific T Lymphocytes
Altman, J. D., Moss, P. A. H., Goulder, P. J. R., Barouch, D. H., McHeyzer-Williams, M. G., Bell, J. I., McMichael, A. J., & Davis, M. M. (1996) · Science · doi:10.1126/science.274.5284.94 · Cited by 3,544 · cited 2×
“However, technical innovations, such as the development of MHC I tetramers (Altman et al., 1996), revealed that antigen-specific CD8 T cell responses were 10- to 100-fold bigger than initially thought, precipitating a substantial revision in conceptualization of the immune response (Murali-Krishna et al.”
BackgroundPreferential Localization of Effector Memory Cells in Nonlymphoid Tissue
Masopust, D., Vezys, V., Marzo, A. L., & Lefrançois, L. (2001) · Science · doi:10.1126/science.1058867 · Cited by 1,952 · cited 2×
“…CD8 T cells are broadly distributed throughout the host organism, but the overall magnitude and anatomic apportionment of this population remain unclear and controversial (Ganusov and De Boer, 2007; Masopust et al., 2001; Peaudecerf and Rocha, 2011; Reinhardt et al., 2001; Rocha et al., 1991).”
BackgroundSelf-citationCounting Antigen-Specific CD8 T Cells: A Reevaluation of Bystander Activation during Viral Infection
Murali‐Krishna, K., Altman, J. D., Suresh, M., Sourdive, D. J. D., Zajac, A. J., Miller, J. D., Slansky, J. E., & Ahmed, R. (1998) · Immunity · doi:10.1016/s1074-7613(00)80470-7 · Cited by 1,918
Lineage relationship and protective immunity of memory CD8 T cell subsets
Wherry, E. J., Teichgräber, V., Becker, T. C., Masopust, D., Kaech, S. M., Antia, R., von Andrian, U. H., & Ahmed, R. (2003) · Nature Immunology · doi:10.1038/ni889 · Cited by 1,864
Self-citationThe developmental pathway for CD103+CD8+ tissue-resident memory T cells of skin
Mackay, L. K., Rahimpour, A., Ma, J. Z., Collins, N., Stock, A. T., Hafon, M.-L., Vega-Ramos, J., Lauzurica, P., Mueller, S. N., Stefanovic, T., et al. (2013) · Nature Immunology · doi:10.1038/ni.2744 · Cited by 1,331
Gebhardt, T., Wakim, L. M., Eidsmo, L., Reading, P. C., Heath, W. R., & Carbone, F. R. (2009) · Nature Immunology · doi:10.1038/ni.1718 · Cited by 1,157
Visualizing the generation of memory CD4 T cells in the whole body
Reinhardt, R. L., Khoruts, A., Merica, R., Zell, T., & Jenkins, M. K. (2001) · Nature · doi:10.1038/35065111 · Cited by 1,026 · cited 1×
“…CD8 T cells are broadly distributed throughout the host organism, but the overall magnitude and anatomic apportionment of this population remain unclear and controversial (Ganusov and De Boer, 2007; Masopust et al., 2001; Peaudecerf and Rocha, 2011; Reinhardt et al., 2001; Rocha et al., 1991).”
BackgroundHuman memory T cells: generation, compartmentalization and homeostasis
Farber, D. L., Yudanin, N. A., & Restifo, N. P. (2014) · Nature Reviews Immunology · doi:10.1038/nri3567 · Cited by 897
Memory T Cell Subsets, Migration Patterns, and Tissue Residence
Mueller, S. N., Gebhardt, T., Carbone, F., & Heath, W. R. (2012) · Annual Review of Immunology · doi:10.1146/annurev-immunol-032712-095954 · Cited by 884
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Human T cell development, localization, and function throughout life
Kumar, B. V., Connors, T. J., & Farber, D. L. (2018) · Immunity · doi:10.1016/j.immuni.2018.01.007 · Cited by 1,257
Kumar, B. V., Ma, W., Miron, M., Granot, T., Guyer, R. S., Carpenter, D. J., Senda, T., Sun, X., Ho, S.-H., Lerner, H., et al. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.08.078 · Cited by 1,068
Normalizing the environment recapitulates adult human immune traits in laboratory mice
Beura, L. K., Hamilton, S. E., Bi, K., Schenkel, J. M., Odumade, O. A., Casey, K. A., Thompson, E. A., Fraser, K. A., Rosato, P. C., Filali-Mouhim, A., et al. (2016) · Nature · doi:10.1038/nature17655 · Cited by 1,072
Self-citationThe Tabula Sapiens: A multiple-organ, single-cell transcriptomic atlas of humans
The Tabula Sapiens Consortium*, Jones, R. C., Karkanias, J., Krasnow, M. A., Pisco, A. O., Quake, S. R., Salzman, J., Yosef, N., Bulthaup, B., Brown, P., et al. (2022) · Science · doi:10.1126/science.abl4896 · Cited by 1,056
Advances in the development of personalized neoantigen-based therapeutic cancer vaccines
Blass, E., & Ott, P. A. (2021) · Nature Reviews Clinical Oncology · doi:10.1038/s41571-020-00460-2 · Cited by 1,042
Metadata
- Authors
- Steinert, E. M., Schenkel, J. M., Fraser, K. A., Beura, L. K., Manlove, L. S., Igyártó, B. Z., Southern, P. J., & Masopust, D.
- Affiliations
- University of Minnesota (US)
- Corresponding author
- Masopust, David
- ORCID
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2015-05
- Volume
- 161
- Issue
- 4
- Pages
- 737-749
- ISSN
- 0092-8674 (ISSN-L)
- PMC
- PMC4426972
- OpenAlex
- W1632859538
- Cited by
- 685
- References
- 46
- Citations by year
- 2026: 29 · 2025: 46 · 2024: 46 · 2023: 50 · 2022: 62 · 2021: 76 · 2020: 74 · 2019: 79 · 2018: 91 · 2017: 60 · 2016: 57 · 2015: 15
- FWCI
- 36.57
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Cancer Institute (F31CA183226, P30CA077598)
- National Institute of Allergy and Infectious Diseases (R01AI084913, R01AI111671, R37AI084913, T32AI083196)
- National Institute of Diabetes and Digestive and Kidney Diseases (F30DK100159, F30-DK100159-02)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Life Sciences › Immunology and Microbiology › Immunology › Immune responses and vaccinations
- Keywords
- memory CD8+ T cells, immune surveillance, parabiosis, lymphocyte isolation, tissue-resident memory T cells, effector memory T cells, central memory T cells, T cell migration, tissue inflammation, T cell differentiation, non-lymphoid tissues
- MeSH terms
- CD69 Antigens, Animals, Antigens, Differentiation, T-Lymphocyte, Arenaviridae Infections, Cell Movement, Immunologic Memory, Inflammation, Lymphocytic choriomeningitis virus, Mice, Inbred C57BL, Monitoring, Immunologic, Antigens, CD, T-Lymphocyte Subsets, CD8-Positive T-Lymphocytes, Lectins, C-Type, Mice
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