Vaginal Lactobacillus fatty acid response mechanisms reveal a metabolite-targeted strategy for bacterial vaginosis treatment
Open accessAbstract
Bacterial vaginosis (BV), a common syndrome characterized by Lactobacillus-deficient vaginal microbiota, is associated with adverse health outcomes. BV often recurs after standard antibiotic therapy in part because antibiotics promote microbiota dominance by Lactobacillus iners instead of Lactobacillus crispatus, which has more beneficial health associations. Strategies to promote L. crispatus and inhibit L. iners are thus needed. We show that oleic acid (OA) and similar long-chain fatty acids simultaneously inhibit L. iners and enhance L. crispatus growth. These phenotypes require OA-inducible genes conserved in L. crispatus and related lactobacilli, including an oleate hydratase (ohyA) and putative fatty acid efflux pump (farE). FarE mediates OA resistance, while OhyA is robustly active in the vaginal microbiota and enhances bacterial fitness by biochemically sequestering OA in a derivative form only ohyA-harboring organisms can exploit. OA promotes L. crispatus dominance more effectively than antibiotics in an in vitro BV model, suggesting a metabolite-based treatment approach.
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Genes related with fatty acid response mechanism were found in all strains.
“genes related with the fatty acid response mechanism, important when vaginal bacterial vaginosis may be treated with oleic acid as alternative to antibiotics [42], were found in all the strains.”
From Women's health is a team effort: probiogenomics supports the development of a multi-species vaginal probiotic (2026) · cites this paper as [42]
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Screening and characterization of vaginal fluid donations for vaginal microbiota transplantation
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Self-citationIdentification and characterization of an oleate hydratase-encoding gene fromBifidobacterium breve
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Genetic Manipulation of Staphylococcus aureus
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Flagged first, then by citations
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Cited by
Sorted by citations
Calvanese, C. M., Valentino, V., De Prisco, A., Allesina, S., Amoruso, A., Deidda, F., Visciglia, A., Ercolini, D., Pane, M., & De Filippis, F. (2026) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-026-06107-2 · Cited by 2 · see citing works
“In addition, genes related with the fatty acid response mechanism, important when vaginal bacterial vaginosis may be treated with oleic acid as alternative to antibiotics [42], were found in all the strains. those involved in biofilm formation and co-aggregation.”
- Authors
- Zhu, M., Frank, M. W., Radka, C. D., Jeanfavre, S., Xu, J., Tse, M. W., Pacheco, J. A., Kim, J. S., Pierce, K., Deik, A., Hussain, F. A., Elsherbini, J., Hussain, S., Xulu, N., Khan, N., Pillay, V., Mitchell, C. M., Dong, K. L., Ndung'u, T., Clish, C. B., Rock, C. O., Blainey, P. C., Bloom, S. M., & Kwon, D. S.
- Affiliations
- Broad Institute (US)
- St. Jude Children's Research Hospital (US)
- University of Kentucky (US)
- Ragon Institute of MGH, MIT and Harvard (US)
- Harvard University (US)
- University of KwaZulu-Natal (ZA)
- Health Systems Trust (ZA)
- Corresponding author
- Blainey, Paul C.
- Bloom, Seth M.
- Kwon, Douglas S.
- ORCID
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2024-09
- Volume
- 187
- Issue
- 19
- Pages
- 5413-5430.e29
- ISSN
- 0092-8674 (ISSN-L)
- PMC
- PMC11429459
- OpenAlex
- W4401698770
- Cited by
- 61
- References
- 112
- Citations by year
- 2026: 27 · 2025: 29 · 2024: 5
- FWCI
- 15.78
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Cell (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- UKnowledge (University of Kentucky) (submittedVersion, cc-by)
- Funders
- National Science Foundation (1745302)
- National Institute of Allergy and Infectious Diseases (5U19AI42780)
- Bill and Melinda Gates Foundation (EC1000, 71852, 71313, 71595, 71803)
- National Institutes of Health (1K08AI171166, 4R00AI166116)
- Harvard University
- St. Jude Children's Research Hospital
- American Lebanese Syrian Associated Charities
- Broad Institute
- Celsius Therapeutics
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Keywords
- bacterial vaginosis, Lactobacillus crispatus, Lactobacillus iners, oleic acid, long-chain fatty acids, vaginal microbiota, bacterial fitness
- MeSH terms
- Lactobacillus crispatus, Anti-Bacterial Agents, Bacterial Proteins, Fatty Acids, Female, Humans, Lactobacillus, Vagina, Vaginosis, Bacterial, Oleic Acid, Microbiota
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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