Fatty acid synthesis is a target for antibacterial activity of unsaturated fatty acids
Open accessAbstract
Long‐chain unsaturated fatty acids, such as linoleic acid, show antibacterial activity and are the key ingredients of antimicrobial food additives and some antibacterial herbs. However, the precise mechanism for this antimicrobial activity remains unclear. We found that linoleic acid inhibited bacterial enoyl‐acyl carrier protein reductase (FabI), an essential component of bacterial fatty acid synthesis, which has served as a promising target for antibacterial drugs. Additional unsaturated fatty acids including palmitoleic acid, oleic acid, linolenic acid, and arachidonic acid also exhibited the inhibition of FabI. However, neither the saturated form (stearic acid) nor the methyl ester of linoleic acid inhibited FabI. These FabI‐inhibitory activities of various fatty acids and their derivatives very well correlated with the inhibition of fatty acid biosynthesis using [ 14 C] acetate incorporation assay, and importantly, also correlated with antibacterial activity. Furthermore, the supplementation with exogenous fatty acids reversed the antibacterial effect of linoleic acid, which showing that it target fatty acid synthesis. Our data demonstrate for the first time that the antibacterial action of unsaturated fatty acids is mediated by the inhibition of fatty acid synthesis.
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The antibacterial action of fatty acids is affected by carbon chain length, saturation and double bond position.
“The antibacterial action of fatty acids is affected by carbon chain length, saturation and double bond position [17].”
From Dietary lipids, gut microbiota and lipid metabolism (2019) · cites this paper as [17]
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- Authors
- Zheng, C. J., Yoo, J.-S., Lee, T.-G., Cho, H.-Y., Kim, Y.-H., & Kim, W.-G.
- Affiliations
- Korea Research Institute of Bioscience and Biotechnology (KR)
- CrystalGenomics (South Korea) (KR)
- Korea Research Institute of Chemical Technology (KR)
- Chungnam National University (KR)
- Corresponding author
- Kim, Won-Gon
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2005-08-26
- Volume
- 579
- Issue
- 23
- Pages
- 5157-5162
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2013592836
- Cited by
- 690
- References
- 1
- Citations by year
- 2026: 32 · 2025: 43 · 2024: 45 · 2023: 43 · 2022: 68 · 2021: 74 · 2020: 57 · 2019: 41 · 2018: 44 · 2017: 40 · 2016: 28 · 2015: 28 · 2014: 29 · 2013: 27 · 2012: 21
- FWCI
- 4.40
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- KRIBB Repository (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Keywords
- unsaturated fatty acids, linoleic acid, antibacterial activity, fatty acid synthesis, FabI, palmitoleic acid, oleic acid, linolenic acid, arachidonic acid, fatty acid synthesis inhibition, fatty acid methyl esters, bacterial growth inhibition, antimicrobial food additives
- MeSH terms
- Anti-Bacterial Agents, Bacteria, Fatty Acids, Fatty Acids, Unsaturated, Oxidoreductases, Enoyl-(Acyl-Carrier-Protein) Reductase (NADH)
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