Cited by 217Immune Response and InflammationGenetics, Aging, and Longevity in Model OrganismsCytokine Signaling Pathways and Interactions

Aging is associated with circulating cytokine dysregulation

Álvarez-Rodríguez, Lorena, López-Hoyos, Marcos, Muñoz-Cacho, Pedro, Martínez-Taboada, Víctor Manuel·2012·Cellular Immunology

Abstract

Purpose Aging is accompanied by a progressive increase in pro-inflammatory cytokine status. However, little is known about the development of age-dependent modifications in other circulating cytokines. The aim of this study was to investigate in vivo the influence of age on circulating cytokine production in healthy subjects (HC).Methods Circulating cytokines were measured by CBA and ELISA in 73 HC. Intracellular cytokine production was assessed in CD3+ and CD14+ cells by flow cytometry. Production of cytokines in cell culture supernatants was also studied after polyclonal stimulation.Results Subjects were divided into three different groups according to age: 28 young HC (<30 years, 26.2 ± 2.4), 24 middle age HC (30-60 years, 44.7 ± 8.4) and 21 elderly HC (>60 years, 70.6 ± 7.9). Age was positively correlated with the circulating levels of IL-12p70, IL-1β, TNFα, IL-6, and IL-10. Age had a negative correlation with circulating levels of IL-17. Besides, age was positively correlated with spontaneous intracellular expression of proinflammatory cytokines in circulating monocytes. No correlation was found with other intracellular cytokine expression or with the production of cytokines in cell culture supernatants after in vitro stimulation. Gender had a marginal effect on the circulating cytokine profile.Conclusion Aging has a significant impact on the production of circulating cytokines in healthy individuals. The circulating cytokine milieu may contribute to the development of age-restricted conditions.

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Crossref · 10.1016/j.cellimm.2012.01.001

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  • Inflammageing involves higher circulating levels of inflammatory mediators such as CRP, IL-6, and TNFα.

    “Inflammageing is characterised by an increase in circulating inflammatory mediators such as C Reactive protein (CRP), Interleukin (IL)-6 and Tumour Necrosis Factor (TNF)α (18).”

    From The impact of ageing on monocytes and macrophages (2021) · cites this paper as [18]

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Authors
Álvarez-Rodríguez, L., López-Hoyos, M., Muñoz-Cacho, P., & Martínez-Taboada, V. M.
Affiliations
Universidad de Cantabria (ES)
Corresponding author
Martínez-Taboada, Víctor Manuel
Venue
Cellular Immunology
Publisher
Elsevier BV
Published
2012
Volume
273
Issue
2
Pages
124-132
ISSN
0008-8749 (ISSN-L)
OpenAlex
W2071079513
Cited by
217
References
41
Citations by year
2026: 11 · 2025: 28 · 2024: 14 · 2023: 9 · 2022: 15 · 2021: 21 · 2020: 11 · 2019: 20 · 2018: 19 · 2017: 18 · 2016: 7 · 2015: 9 · 2014: 11 · 2013: 20 · 2012: 4
FWCI
6.13
Type
journal article
Language
en
Open access
closed
Locations
  • Cellular Immunology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Fundación Marqués de Valdecilla
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
  • Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
Keywords
aging, serum cytokines, inflammatory cytokines, IL-12p70, IL-1β, TNF-α, interleukin-6, IL-10, IL-17, monocytes, intracellular cytokine expression, healthy older adults, age-related inflammation
MeSH terms
Adult, Aged, Aged, 80 and over, Aging, Cells, Cultured, Enzyme-Linked Immunosorbent Assay, Female, Flow Cytometry, Humans, Male, Middle Aged, Monocytes, T-Lymphocytes, Cytokines, CD3 Complex, Lipopolysaccharide Receptors, Th1-Th2 Balance

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