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Cited by 71Food Allergy and Anaphylaxis ResearchGastrointestinal motility and disordersEosinophilic Esophagitis

A Cytologic Assay for Diagnosis of Food Hypersensitivity in Patients With Irritable Bowel Syndrome

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Carroccio, Antonio, Brusca, Ignazio, Mansueto, Pasquale, Pirrone, Giuseppe, Barrale, Maria, Di Prima, Lidia, Ambrosiano, Giuseppe, Iacono, Giuseppe +3 more·2010·Clinical Gastroenterology and Hepatology
DOI 10.1016/j.cgh.2009.11.010
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Abstract

Background & aims A percentage of patients with symptoms of irritable bowel syndrome (IBS) suffer from food hypersensitivity (FH) and improve on a food-elimination diet. No assays have satisfactory levels of sensitivity for identifying patients with FH. We evaluated the efficacy of an in vitro basophil activation assay in the diagnosis of FH in IBS-like patients.Methods Blood samples were collected from 120 consecutive patients diagnosed with IBS according to Rome II criteria. We analyzed in vitro activation of basophils by food allergens (based on levels of CD63 expression), as well as total and food-specific immunoglobulin (Ig)E levels in serum. Effects of elimination diets and double-blind food challenges were used as standards for FH diagnosis.Results Twenty-four of the patients (20%) had FH (cow's milk and/or wheat hypersensitivity); their symptom scores improved significantly when they were placed on an elimination diet. Patients with FH differed from other IBS patients in that they had a longer duration of clinical history, a history of FH as children, and an increased frequency of self-reported FH; they also had hypersensitivities to other antigens (eg, egg or soy). The basophil activation assay diagnosed FH with 86% sensitivity, 88% specificity, and 87% accuracy; this level of sensitivity was significantly higher than that of serum total IgE or food-specific IgE assays.Conclusions A cytometric assay that quantifies basophils after stimulation with food antigens based on cell-surface expression of CD63 had high levels of sensitivity, specificity, and accuracy in diagnosing FH. This assay might be used to diagnose FH in patients with IBS-like symptoms.

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Crossref · 10.1016/j.cgh.2009.11.010

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  • Few studies demonstrated increased peripheral blood basophil activation following stimulation with food antigens and elevated levels of fecal tryptase and eosinophil cationic protein in patients with IBS and food hypersensitivity.

    “In addition, few studies demonstrated increased peripheral blood basophil activation following stimulation with food antigens (30) and elevated levels of fecal tryptase and eosinophil cationic protein (31) in patients with IBS and food hypersensitivity, thus further suggesting possible inflammatory and altered immune etiology.”

    From Intestinal microbiota and immune function in the pathogenesis of irritable bowel syndrome (2013) · cites this paper as [30]

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Authors
Carroccio, A., Brusca, I., Mansueto, P., Pirrone, G., Barrale, M., Di Prima, L., Ambrosiano, G., Iacono, G., Lospalluti, M. L., La Chiusa, S. M., & Di Fede, G.
Affiliations
  • University of Palermo (IT)
  • Ospedale Buccheri la Ferla Fatebenefratelli (IT)
Corresponding author
Carroccio, Antonio
ORCID
Venue
Clinical Gastroenterology and Hepatology
Publisher
Elsevier BV
Published
2010-03
Volume
8
Issue
3
Pages
254-260
ISSN
1542-3565 (ISSN-L)
DOI
10.1016/j.cgh.2009.11.010
OpenAlex
W2150066607
Cited by
71
References
35
Citations by year
2025: 1 · 2024: 3 · 2023: 2 · 2022: 1 · 2021: 5 · 2020: 2 · 2019: 2 · 2018: 4 · 2017: 3 · 2016: 4 · 2015: 14 · 2014: 7 · 2013: 9 · 2012: 4
FWCI
3.70
Type
journal article
Language
en
Open access
Licence
Locations
  • Clinical Gastroenterology and Hepatology (publishedVersion)
  • PubMed (publishedVersion)
  • Nova Science Publishers (Nova Science Publishers, Inc.) (submittedVersion)
  • INFM-OAR (INFN Catania) (submittedVersion, other-oa)
  • Zenodo (CERN European Organization for Nuclear Research) (other-oa)
Topics
  • Health Sciences › Medicine › Immunology and Allergy › Food Allergy and Anaphylaxis Research
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
  • Health Sciences › Medicine › Surgery › Eosinophilic Esophagitis
Keywords
food allergy, irritable bowel syndrome, CD63, food allergens, cow's milk allergy, wheat allergy, food-specific IgE, elimination diet, sensitivity and specificity
MeSH terms
Adolescent, Adult, Allergens, Animals, Basophils, Cells, Cultured, Cytological Techniques, Female, Food Hypersensitivity, Humans, Immunoglobulin E, Male, Middle Aged, Platelet Membrane Glycoproteins, Sensitivity and Specificity, Antigens, CD, Irritable Bowel Syndrome, Young Adult, Tetraspanin 30

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