Plant-derived exosomal MicroRNAs shape the gut microbiota
Open accessAbstract
The gut microbiota can be altered by dietary interventions to prevent and treat various diseases. However, the mechanisms by which food products modulate commensals remain largely unknown. We demonstrate that plant-derived exosome-like nanoparticles (ELNs) are taken up by the gut microbiota and contain RNAs that alter microbiome composition and host physiology. Ginger ELNs (GELNs) are preferentially taken up by Lactobacillaceae in a GELN lipid-dependent manner and contain microRNAs that target various genes in Lactobacillus rhamnosus (LGG). Among these, GELN mdo-miR7267-3p-mediated targeting of the LGG monooxygenase ycnE yields increased indole-3-carboxaldehyde (I3A). GELN-RNAs or I3A, a ligand for aryl hydrocarbon receptor, are sufficient to induce production of IL-22, which is linked to barrier function improvement. These functions of GELN-RNAs can ameliorate mouse colitis via IL-22-dependent mechanisms. These findings reveal how plant products and their effects on the microbiome may be used to target specific host processes to alleviate disease.
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AhR activation by FICZ was therapeutic in the dextran sodium sulfate-induced mouse colitis model in which FICZ treatment upregulated tight junction protein expression in inflamed mouse colons.
“AhR activation by FICZ was therapeutic in the dextran sodium sulfate (DSS)–induced mouse colitis model in which FICZ treatment upregulated TJ protein expression in inflamed mouse colons.92,93”
From Regulation of Intestinal Barrier Function by Microbial Metabolites (2021) · cites this paper as [93]
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- Authors
- Teng, Y., Ren, Y., Sayed, M., Hu, X., Lei, C., Kumar, A., Hutchins, E., Mu, J., Deng, Z., Luo, C., Sundaram, K., Sriwastva, M. K., Zhang, L., Hsieh, M., Reiman, R., Haribabu, B., Yan, J., Jala, V. R., Miller, D. M., Van Keuren-Jensen, K., Merchant, M. L., McClain, C. J., Park, J. W., Egilmez, N. K., & Zhang, H.-G.
- Affiliations
- University of Louisville (US)
- Huaian First People’s Hospital (CN)
- The University of Texas MD Anderson Cancer Center (US)
- Translational Genomics Research Institute (US)
- Corresponding author
- Teng, Yun
- Zhang, Huang-Ge
- ORCID
- Venue
- Cell Host & Microbe
- Publisher
- Elsevier BV
- Published
- 2018-11
- Volume
- 24
- Issue
- 5
- Pages
- 637-652.e8
- ISSN
- 1931-3128 (ISSN-L)
- PMC
- PMC6746408
- OpenAlex
- W2898380126
- Cited by
- 1,027
- References
- 31
- Citations by year
- 2026: 191 · 2025: 158 · 2024: 183 · 2023: 158 · 2022: 111 · 2021: 114 · 2020: 71 · 2019: 38 · 2018: 2 · 2017: 1
- FWCI
- 20.01
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell Host & Microbe (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- NIH (UH3TR000875, R01AT008617)
- Robley Rex VA Medical Center Merit Review Grant (1P30GM106396)
- Molecular Targets Phase III COBRE grant
- National Institute of General Medical Sciences (P20GM113226-6174)
- National Institutes of Health (P20GM103436)
- National Institute of Alcohol Abuse and Alcoholism (P50 AA024337)
- National Institute of General Medical Sciences of the National Institutes of Health (P20 GM113226)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Keywords
- exosome-like nanoparticles, plant-derived microRNAs, gut microbiota, ginger, Lactobacillus rhamnosus, Lactobacillaceae, indole-3-carboxaldehyde, aryl hydrocarbon receptor, IL-22, intestinal barrier function, colitis
- MeSH terms
- Gastrointestinal Microbiome, Interleukin-22, LexA Repressor Protein, Animals, Bacterial Proteins, Colitis, Disease Models, Animal, Disease Susceptibility, Female, Food, Germ-Free Life, Indoles, Interleukins, Intestines, Male, Mice, Inbred C57BL, Plants, RNA, Ribosomal, 16S, Serine Endopeptidases, Tryptophan, Receptors, Aryl Hydrocarbon, Immunity, Mucosal, MicroRNAs, Mice, Lacticaseibacillus rhamnosus, Host-Pathogen Interactions, Exosome Multienzyme Ribonuclease Complex
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