Cited by 70Gut microbiota and healthInflammatory Bowel DiseaseImmune Response and Inflammation

Antimicrobial overproduction sustains intestinal inflammation by inhibiting Enterococcus colonization

Open access
Jang, Kyung Ku, Heaney, Thomas, London, Mariya, Ding, Yi, Putzel, Gregory, Yeung, Frank, Ercelen, Defne, Chen, Ying-Han +13 more·2023·Cell Host & Microbe

Abstract

Loss of antimicrobial proteins such as REG3 family members compromises the integrity of the intestinal barrier. Here, we demonstrate that overproduction of REG3 proteins can also be detrimental by reducing a protective species in the microbiota. Patients with inflammatory bowel disease (IBD) experiencing flares displayed heightened levels of secreted REG3 proteins that mediated depletion of Enterococcus faecium (Efm) from the gut microbiota. Efm inoculation of mice ameliorated intestinal inflammation through activation of the innate immune receptor NOD2, which was associated with the bacterial DL-endopeptidase SagA that generates NOD2-stimulating muropeptides. NOD2 activation in myeloid cells induced interleukin-1β (IL-1β) secretion to increase the proportion of IL-22-producing CD4+ T helper cells and innate lymphoid cells that promote tissue repair. Finally, Efm was unable to protect mice carrying a NOD2 gene variant commonly found in IBD patients. Our findings demonstrate that inflammation self-perpetuates by causing aberrant antimicrobial activity that disrupts symbiotic relationships with gut microbes.

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Crossref · 10.1016/j.chom.2023.08.002

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  • Current clinical proxies for dysbiosis include MAdCAM-1, REG3, calprotectin, and lipocalin-2 in fecal samples.

    “Current clinical proxies for dysbiosis include MAdCAM-1, REG3, calprotectin, and lipocalin-2 in fecal samples.52,157,158”

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Authors
Jang, K. K., Heaney, T., London, M., Ding, Y., Putzel, G., Yeung, F., Ercelen, D., Chen, Y.-H., Axelrad, J., Gurunathan, S., Zhou, C., Podkowik, M., Arguelles, N., Srivastava, A., Shopsin, B., Torres, V. J., Keestra-Gounder, A. M., Pironti, A., Griffin, M. E., Hang, H. C., & Cadwell, K.
Affiliations
  • New York University (US)
  • Geisinger Health System (US)
  • NYU Langone Health (US)
  • University of Pennsylvania (US)
  • University of Colorado Anschutz (US)
  • Scripps Research Institute (US)
  • University of Pennsylvania Health System (US)
Corresponding author
Cadwell, Ken
Venue
Cell Host & Microbe
Publisher
Elsevier BV
Published
2023-09
Volume
31
Issue
9
Pages
1450-1468.e8
ISSN
1931-3128 (ISSN-L)
OpenAlex
W4386295669
Cited by
70
References
116
Citations by year
2026: 22 · 2025: 23 · 2024: 20 · 2023: 5
FWCI
6.22
Type
journal article
Language
en
Open access
Locations
  • Cell Host & Microbe (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • PubMed Central (submittedVersion)
Funders
  • Howard Hughes Medical Institute
  • Kenneth Rainin Foundation
  • Crohn's and Colitis Foundation
  • National Institutes of Health (1s10od018338-01)
  • School of Medicine, New York University
  • NYU Grossman School of Medicine
  • National Heart, Lung, and Blood Institute (R01HL123340)
  • National Cancer Institute (P30CA016087)
  • National Institute of Allergy and Infectious Diseases (R01AI140754, R01AI137336, R01AI130945, R21AI164154, R01AI121244, R01AI173121)
  • National Institute of Diabetes and Digestive and Kidney Diseases (F32 DK132908, F30DK122698, R01DK124336, R01DK093668)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
Keywords
Enterococcus faecium, intestinal inflammation, inflammatory bowel disease, NOD2, muropeptides, IL-1β, IL-22, CD4+ T helper cells, innate lymphoid cells, gut microbiota, intestinal barrier, symbiosis, NOD2 variants

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