Cited by 55Microfluidic and Capillary Electrophoresis ApplicationsAnalytical chemistry methods developmentAnalytical Chemistry and Chromatography

Analysis of cytokinin nucleotides in coconut (Cocos nucifera L.) water using capillary zone electrophoresis-tandem mass spectrometry after solid-phase extraction

Ge, Liya, Yong, Jean Wan Hong, Tan, Swee Ngin, Yang, Xin Hao, Ong, Eng Shi·2006·Journal of Chromatography A

Abstract

A method based on solid-phase extraction (SPE) and capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) is described for the separation and determination of six cytokinin nucleotides in coconut water. The best CZE separation for the six cytokinin nucleotide standards was achieved using a 25 mM ammonium formate/formic acid buffer (pH 3.8) and 2% (v/v) methanol with an applied gradient separation voltage (25 kV for 32 min, and then a linear gradient to 30 kV in 5 min, finally 30 kV to the end of separation) in less than 60 min. MS/MS with multiple reaction monitoring (MRM) detection was carried out to obtain sufficient selectivity and sensitivity for the cytokinin nucleotides. The combined use of on-line sample stacking and CZE-MS/MS achieved limits of detection (LODs) in the range of 0.06-0.19 microM for the six cytokinin nucleotides at a signal-to-noise ratio of 3. Furthermore, a novel dual-step SPE procedure was developed for the pre-concentration and purification of cytokinin nucleotides using Oasis HLB and Oasis MAX cartridges. The recoveries of the cytokinin nucleotides after the dual-step SPE were in the range of 44-71%. The combination of off-line SPE, on-line sample stacking and CZE-MS/MS approach was successfully applied to screen for endogenous cytokinin nucleotides present in coconut water sample. trans-Zeatin riboside-5'-monophosphate (ZMP) was detected and quantified in coconut water by CZE-MS/MS after SPE and on-line sample stacking.

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Crossref · 10.1016/j.chroma.2006.08.027

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Authors
Ge, L., Yong, J. W. H., Tan, S. N., Yang, X. H., & Ong, E. S.
Affiliations
  • Nanyang Technological University (SG)
  • National University of Singapore (SG)
Corresponding author
Tan, Swee Ngin
Venue
Journal of Chromatography A
Publisher
Elsevier BV
Published
2006-11
Volume
1133
Issue
1-2
Pages
322-331
ISSN
0021-9673 (ISSN-L)
OpenAlex
W2005871135
Cited by
55
References
43
Citations by year
2025: 1 · 2024: 5 · 2022: 3 · 2021: 2 · 2019: 1 · 2018: 4 · 2017: 3 · 2015: 2 · 2014: 2 · 2013: 4 · 2012: 4
FWCI
2.56
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Chromatography A (publishedVersion)
  • PubMed (publishedVersion)
  • National University of Singapore (submittedVersion)
Funders
Nanyang Technological University
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
  • Physical Sciences › Chemistry › Analytical Chemistry › Analytical chemistry methods development
  • Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
Keywords
coconut water, Cocos nucifera, solid-phase extraction, multiple reaction monitoring
MeSH terms
Cocos, Cytokinins, Mass Spectrometry, Reproducibility of Results, Molecular Structure, Electrophoresis, Capillary, Spectrometry, Mass, Electrospray Ionization

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