Non-Canonical Control of Neuronal Energy Status by the Na+ Pump
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Neurons have limited intracellular energy stores but experience acute and unpredictable increases in energy demand. To better understand how these cells repeatedly transit from a resting to active state without undergoing metabolic stress, we monitored their early metabolic response to neurotransmission using ion-sensitive probes and FRET sensors in vitro and in vivo. A short theta burst triggered immediate Na+ entry, followed by a delayed stimulation of the Na+/K+ ATPase pump. Unexpectedly, cytosolic ATP and ADP levels were unperturbed across a wide range of physiological workloads, revealing strict flux coupling between the Na+ pump and mitochondria. Metabolic flux measurements revealed a "priming" phase of mitochondrial energization by pyruvate, whereas glucose consumption coincided with delayed Na+ pump stimulation. Experiments revealed that the Na+ pump plays a permissive role for mitochondrial ATP production and glycolysis. We conclude that neuronal energy homeostasis is not mediated by adenine nucleotides or by Ca 2+, but by a mechanism commanded by the Na+ pump.
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In vitro studies have shown that neuronal ATP levels decrease only under hyperexcitation induced by strong electrical stimulation or exogenous glutamate exposure, or following pharmacological inhibition of mitochondrial ATP production pathways.
“Indeed, in vitro studies have shown that neuronal ATP levels decrease only under hyperexcitation induced by strong electrical stimulation or exogenous glutamate exposure, or following pharmacological inhibition of mitochondrial ATP production pathways20–22.”
From Energy paradox in REM sleep: balancing supply and consumption in brain metabolism (2026) · cites this paper as [22]
In vivo studies have demonstrated that neuronal ATP levels remain stable in response to mild sensory stimuli in mice.
“Consistent with these findings, in vivo studies have demonstrated that neuronal ATP levels remain stable in response to mild sensory stimuli in mice22, while a marked reduction in ATP levels is observed during epileptic seizure activity3.”
From Energy paradox in REM sleep: balancing supply and consumption in brain metabolism (2026) · cites this paper as [22]
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- Authors
- Baeza-Lehnert, F., Saab, A. S., Gutiérrez, R., Larenas, V., Díaz, E., Horn, M., Vargas, M., Hösli, L., Stobart, J., Hirrlinger, J., Weber, B., & Barros, L. F.
- Affiliations
- University of Zurich (CH)
- Austral University of Chile (CL)
- Centro de Estudios Científicos (CL)
- Max Planck Institute of Experimental Medicine (DE)
- Corresponding author
- Barros, L. Felipe
- ORCID
- Venue
- Cell Metabolism
- Publisher
- Elsevier BV
- Published
- 2019-03
- Volume
- 29
- Issue
- 3
- Pages
- 668-680.e4
- ISSN
- 1550-4131 (ISSN-L)
- OpenAlex
- W2904685632
- Cited by
- 126
- References
- 78
- Citations by year
- 2026: 10 · 2025: 19 · 2024: 25 · 2023: 21 · 2022: 12 · 2021: 12 · 2020: 13 · 2019: 14
- FWCI
- 3.47
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Zurich Open Repository and Archive (University of Zurich) (submittedVersion)
- Funders
- FONDECYT (1160317)
- University of Zurich
- National Science Foundation
- Centro de Estudios Científicos
- Chilean Government
- CONICYT
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Keywords
- Na+/K+-ATPase, mitochondrial energization, pyruvate, glycolysis, glucose utilization, ATP production, neurotransmission, theta burst stimulation, FRET biosensor, ion sensitive probe
- MeSH terms
- Sodium-Potassium-Exchanging ATPase, Adenosine Triphosphate, Animals, Astrocytes, Energy Metabolism, Glucose, Glycolysis, Homeostasis, Mice, Inbred C57BL, Mitochondria, Neurons
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