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Cited by 71Neuroscience and Neuropharmacology ResearchNeuroinflammation and Neurodegeneration MechanismsNeurogenesis and neuroplasticity mechanisms

Astrocyte-neuron crosstalk regulates the expression and subcellular localization of carbohydrate metabolism enzymes

Mamczur, Piotr, Borsuk, Borys, Paszko, Jadwiga, Sas, Zuzanna, Mozrzymas, Jerzy, Wiśniewski, Jacek R., Gizak, Agnieszka, Rakus, Dariusz·2015·Glia
DOI 10.1002/glia.22753
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Abstract

Astrocytes releasing glucose- and/or glycogen-derived lactate and glutamine play a crucial role in shaping neuronal function and plasticity. Little is known, however, how metabolic functions of astrocytes, e.g., their ability to degrade glucosyl units, are affected by the presence of neurons. To address this issue we carried out experiments which demonstrated that co-culturing of rat hippocampal astrocytes with neurons significantly elevates the level of mRNA and protein for crucial enzymes of glycolysis (phosphofructokinase, aldolase, and pyruvate kinase), glycogen metabolism (glycogen synthase and glycogen phosphorylase), and glutamine synthetase in astrocytes. Simultaneously, the decrease of the capability of neurons to metabolize glucose and glutamine is observed. We provide evidence that neurons alter the expression of astrocytic enzymes by secretion of as yet unknown molecule(s) into the extracellular fluid. Moreover, our data demonstrate that almost all studied enzymes may localize in astrocytic nuclei and this localization is affected by the co-culturing with neurons which also reduces proliferative activity of astrocytes. Our results provide the first experimental evidence that the astrocyte-neuron crosstalk substantially affects the expression of basal metabolic enzymes in the both types of cells and influences their subcellular localization in astrocytes.

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  • In vitro glycolytic behavior can change with choice of culture media and astrocyte coculture.

    “In vitro glycolytic behavior can change with choice of culture media (Sünwoldt et al., 2017) and astrocyte coculture (Mamczur et al., 2015), but gene expression data from acutely dissociated brain cells indicate that neurons are well equipped for glycolysis, with higher levels of mRNA expression than astrocytes for most glycolytic enzymes (Fig. 1, left).”

    From Fueling thought: Management of glycolysis and oxidative phosphorylation in neuronal metabolism (2018) · cites this paper as Mamczur et al., 2015

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Authors
Mamczur, P., Borsuk, B., Paszko, J., Sas, Z., Mozrzymas, J., Wiśniewski, J. R., Gizak, A., & Rakus, D.
Affiliations
  • University of Wrocław (PL)
  • Max Planck Institute of Biochemistry (DE)
Corresponding author
Rakus, Dariusz
ORCID
Venue
Glia
Publisher
Wiley
Published
2014-09-24
Volume
63
Issue
2
Pages
328-340
ISSN
  • 0894-1491 (ISSN-L)
  • 1098-1136
DOI
10.1002/glia.22753
OpenAlex
W1952806621
Cited by
71
References
57
Citations by year
2026: 2 · 2025: 5 · 2024: 2 · 2023: 6 · 2022: 7 · 2021: 5 · 2020: 9 · 2019: 7 · 2018: 11 · 2017: 4 · 2016: 5 · 2015: 8
FWCI
2.59
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Glia (publishedVersion)
  • PubMed (publishedVersion)
  • Max Planck Digital Library (submittedVersion)
  • MPG.PuRe (Max Planck Society) (submittedVersion)
Funders
  • Polish Ministry of Science and Higher Education (#N303815540)
  • Experimental Biology Institute of Wrocław University
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords
astrocyte-neuron crosstalk, astrocyte metabolism, carbohydrate metabolism enzymes, glycolysis, glycogen metabolism, glutamine metabolism, lactate, glutamine synthetase, phosphofructokinase, aldolase, pyruvate kinase, glycogen synthase, glycogen phosphorylase, subcellular localization, nuclear localization, astrocyte proliferation, hippocampal astrocytes, co-culture
MeSH terms
Actins, Animals, Animals, Newborn, Astrocytes, Cells, Cultured, Fructose-Bisphosphate Aldolase, Glycogen Synthase, Hippocampus, Neurons, Pyruvate Kinase, Subcellular Fractions, Culture Media, Conditioned, Rats, Wistar, Coculture Techniques, Proteomics, Carbohydrate Metabolism, Rats

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