Cited by 188Neutrophil, Myeloperoxidase and Oxidative MechanismsRedox biology and oxidative stressNitric Oxide and Endothelin Effects

The phagocyte NOX2 NADPH oxidase in microbial killing and cell signaling

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Nauseef, William M·2019·Current Opinion in Immunology

Abstract

The phagocyte NADPH oxidase possesses a transmembrane electron transferase comprised of gp91phox (aka NOX2) and p22phox and two multicomponent cytosolic complexes, which in stimulated phagocytes translocate to assemble a functional enzyme complex at plasma or phagosomal membranes. The NOX2-centered NADPH oxidase shuttles electrons from cytoplasmic NADPH to molecular oxygen in phagosomes or the extracellular space to produce oxidants that support optimal antimicrobial activity by phagocytes. Additionally, NOX2-generated oxidants have been implicated in both autocrine and paracrine signaling in a variety of biological contexts. However, when interpreting experimental results, investigators must recognize the complexity inherent in the biochemistry of oxidant-mediated attack of microbial targets and the technical limitations of the probes currently used to detect intracellular oxidants.

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Crossref · 10.1016/j.coi.2019.05.006

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  • After activation, neutrophils produce more O2•− compared to monocytes and macrophages.

    “After activation, neutrophils produce more O2•− compared to monocytes and macrophages [178].”
  • In neutrophils, H2O2 generation in phagosomes activates myeloperoxidase, catalysing production of HOCl, which is oxidant and antimicrobial and contributes greatly to lysis of microbes.

    “In neutrophils, H2O2 generation in phagosomes activates the enzyme myeloperoxidase, catalysing the production of HOCl, which is oxidant and antimicrobial, and contributes greatly to the lysis of microbes [178].”

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  1. [42]Forbes LV, Kettle AJ: Myeloperoxidase: Unleashing the Power of Hydrogen Peroxide In Hydrogen Peroxide Metabolism in Health and Disease. Edited by Vissers MCM, Hampton MB, Kettle AJ: CRC Press; 2018: 281– 304. [Packer L, Cadenas E (Series Editor): Oxidative Stress and Disease

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Authors
Nauseef, W. M.
Affiliations
University of Iowa (US)
Corresponding author
Nauseef, William M
Venue
Current Opinion in Immunology
Publisher
Elsevier BV
Published
2019-10
Volume
60
Pages
130-140
ISSN
0952-7915 (ISSN-L)
OpenAlex
W2958386701
Cited by
188
References
85
Citations by year
2026: 33 · 2025: 29 · 2024: 27 · 2023: 34 · 2022: 27 · 2021: 20 · 2020: 16 · 2019: 2
FWCI
1.40
Type
journal article
Language
en
Open access
Locations
  • Current Opinion in Immunology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • U.S. Department of Veterans Affairs
  • National Institutes of Health (R01-AI116546, R01-AI132335)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
Keywords
NADPH oxidase, gp91phox, p22phox, phagocytes, reactive oxygen species, phagosome, autocrine signaling, paracrine signaling, electron transfer
MeSH terms
NADPH Oxidase 2, Animals, Granulomatous Disease, Chronic, Humans, Hydrogen Peroxide, Neutrophils, Oxidation-Reduction, Phagocytes, Phagocytosis, Phagosomes, Signal Transduction, Oxidants

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