Recent advances in prime editing technologies and their promises for therapeutic applications

Search-and-replace genome editing without double-strand breaks or donor DNA

Anzalone, A. V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram, A., et al. (2019) · Nature · doi:10.1038/s41586-019-1711-4 · Cited by 4,767 · see citing works

Prime genome editing in rice and wheat

Lin, Q., Zong, Y., Xue, C., Wang, S., Jin, S., Zhu, Z., Wang, Y., Anzalone, A. V., Raguram, A., Doman, J. L., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0455-x · Cited by 873 · see citing works

Enhanced prime editing systems by manipulating cellular determinants of editing outcomes

Chen, P. J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C.-D., Nelson, J. W., Newby, G. A., Şahin, M., et al. (2021) · Cell · doi:10.1016/j.cell.2021.09.018 · Cited by 820 · see citing works

Engineered pegRNAs improve prime editing efficiency

Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, A. V., An, M., Newby, G. A., Chen, J. C., Hsu, A., et al. (2022) · Nature Biotechnology · doi:10.1038/s41587-021-01039-7 · Cited by 727 · see citing works

Prime editing for precise and highly versatile genome manipulation

Chen, P. J., & Liu, D. R. (2022) · Nature Reviews Genetics · doi:10.1038/s41576-022-00541-1 · Cited by 621 · see citing works

Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing

Anzalone, A. V., Gao, X. D., Podracky, C. J., Nelson, A. T., Koblan, L. W., Raguram, A., Levy, J. M., Mercer, J. A. M., & Liu, D. R. (2022) · Nature Biotechnology · doi:10.1038/s41587-021-01133-w · Cited by 613 · see citing works

Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases

Yarnall, M. T. N., Ioannidi, E. I., Schmitt-Ulms, C., Krajeski, R. N., Lim, J., Villiger, L., Zhou, W., Jiang, K., Garushyants, S. K., Roberts, N., et al. (2022) · Nature Biotechnology · doi:10.1038/s41587-022-01527-4 · Cited by 490 · see citing works

High-efficiency prime editing with optimized, paired pegRNAs in plants

Lin, Q., Jin, S., Zong, Y., Yu, H., Zhu, Z., Liu, G., Kou, L., Wang, Y., Qiu, J.-L., Li, J., et al. (2021) · Nature Biotechnology · doi:10.1038/s41587-021-00868-w · Cited by 325 · see citing works

Phage-assisted evolution and protein engineering yield compact, efficient prime editors

Doman, J. L., Pandey, S., Neugebauer, M. E., An, M., Davis, J. R., Randolph, P. B., McElroy, A. N., Gao, X. D., Raguram, A., Richter, M. F., et al. (2023) · Cell · doi:10.1016/j.cell.2023.07.039 · Cited by 318 · see citing works

Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice

Liu, P., Liang, S.-Q., Zheng, C., Mintzer, E., Zhao, Y. G., Ponnienselvan, K., Mir, A., Sontheimer, E. J., Gao, G., Flotte, T. R., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-22295-w · Cited by 273 · see citing works

Predicting the efficiency of prime editing guide RNAs in human cells

Kim, H. K., Yu, G., Park, J., Min, S., Lee, S., Yoon, S., & Kim, S. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0677-y · Cited by 272 · see citing works

Precise genomic deletions using paired prime editing

Choi, J., Chen, W., Suiter, C. C., Lee, C., Chardon, F. M., Yang, W., Leith, A., Daza, R. M., Martin, B., & Shendure, J. (2021) · Nature Biotechnology · doi:10.1038/s41587-021-01025-z · Cited by 253 · see citing works

An engineered prime editor with enhanced editing efficiency in plants

Zong, Y., Liu, Y., Xue, C., Li, B., Li, X., Wang, Y., Li, J., Liu, G., Huang, X., Cao, X., et al. (2022) · Nature Biotechnology · doi:10.1038/s41587-022-01254-w · Cited by 248 · see citing works

Prime editing for functional repair in patient-derived disease models

Schene, I. F., Joore, I. P., Oka, R., Mokrý, M., van Vugt, A. H. M., van Boxtel, R., van der Doef, H. P. J., van der Laan, L. J. W., Verstegen, M. M. A., van Hasselt, P. M., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-19136-7 · Cited by 237 · see citing works

Efficient prime editing in mouse brain, liver and heart with dual AAVs

Davis, J. R., Banskota, S., Levy, J. M., Newby, G. A., Wang, X., Anzalone, A. V., Nelson, A. T., Chen, P. J., Hennes, A. D., An, M., et al. (2023) · Nature Biotechnology · doi:10.1038/s41587-023-01758-z · Cited by 237 · see citing works

Genotoxic effects of base and prime editing in human hematopoietic stem cells

Fiumara, M., Ferrari, S., Omer-Javed, A., Beretta, S., Albano, L., Canarutto, D., Varesi, A., Gaddoni, C., Brombin, C., Cugnata, F., et al. (2024) · Nature Biotechnology · doi:10.1038/s41587-023-01915-4 · Cited by 220 · see citing works

Efficient generation of mouse models with the prime editing system

Liu, Y., Li, X., He, S., Huang, S., Li, C., Chen, Y., Liu, Z., Huang, X., & Wang, X. (2020) · Cell Discovery · doi:10.1038/s41421-020-0165-z · Cited by 213 · see citing works

Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome

Durrant, M. G., Fanton, A., Tycko, J., Hinks, M. M., Chandrasekaran, S. S., Perry, N. T., Schaepe, J. M., Du, P. P., Lotfy, P., Bassik, M. C., et al. (2022) · Nature Biotechnology · doi:10.1038/s41587-022-01494-w · Cited by 212 · see citing works

CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells

Petri, K., Zhang, W., Ma, J., Schmidts, A., Lee, H., Horng, J. E., Kim, D. Y., Kurt, I. C., Clement, K., Hsu, J. Y., et al. (2022) · Nature Biotechnology · doi:10.1038/s41587-021-00901-y · Cited by 208 · see citing works

Precise integration of large DNA sequences in plant genomes using PrimeRoot editors

Sun, C., Yuan, L., Li, B., Gao, Q., Li, Y., Cao, W., Yang, C., Li, H., Wang, Z., Li, Y., et al. (2023) · Nature Biotechnology · doi:10.1038/s41587-023-01769-w · Cited by 206 · see citing works

Deletion and replacement of long genomic sequences using prime editing

Jiang, T., Zhang, X.-O., Weng, Z., & Xue, W. (2021) · Nature Biotechnology · doi:10.1038/s41587-021-01026-y · Cited by 204 · see citing works

In vivo prime editing of a metabolic liver disease in mice

Böck, D., Rothgangl, T., Villiger, L., Schmidheini, L., Matsushita, M., Mathis, N., Ioannidi, E. I., Rimann, N., Grisch‐Chan, H. M., Kreutzer, S., et al. (2022) · Science Translational Medicine · doi:10.1126/scitranslmed.abl9238 · Cited by 199 · see citing works

PrimeDesign software for rapid and simplified design of prime editing guide RNAs

Hsu, J. Y., Grünewald, J., Szalay, R. N., Shih, J., Anzalone, A. V., Lam, K. C., Shen, M. W., Petri, K., Liu, D. R., Joung, J. K., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-21337-7 · Cited by 189 · see citing works

Application of prime editing to the correction of mutations and phenotypes in adult mice with liver and eye diseases

Jang, H., Jo, D. H., Cho, C. S., Shin, J. H., Seo, J. H., Yu, G., Gopalappa, R., Kim, D., Cho, S., Kim, J. H., et al. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00788-9 · Cited by 184 · see citing works

Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair

da Silva, J. F., Oliveira, G. P., Arasa-Verge, E. A., Kagiou, C., Moretton, A., Timelthaler, G., Jiricny, J., & Loizou, J. I. (2022) · Nature Communications · doi:10.1038/s41467-022-28442-1 · Cited by 182 · see citing works

Dual-AAV delivering split prime editor system for in vivo genome editing

Zhi, S., Chen, Y., Wu, G., Wen, J., Wu, J., Liu, Q., Li, Y., Kang, R., Hu, S., Wang, J., et al. (2022) · Molecular Therapy · doi:10.1016/j.ymthe.2021.07.011 · Cited by 174 · see citing works

Efficient targeted insertion of large DNA fragments without DNA donors

Wang, J., Zhou, H., Wang, G., Zhang, R., Duan, J., Gao, P., Lei, X., Qiu, H.-Y., Zhang, C., Zhang, Y., et al. (2022) · Nature Methods · doi:10.1038/s41592-022-01399-1 · Cited by 173 · see citing works

Designing and executing prime editing experiments in mammalian cells

Doman, J. L., Sousa, A. A., Randolph, P. B., Chen, P. J., & Liu, D. R. (2022) · Nature Protocols · doi:10.1038/s41596-022-00724-4 · Cited by 172 · see citing works

A split prime editor with untethered reverse transcriptase and circular RNA template

Liu, B., Dong, X., Cheng, H., Zheng, C., Chen, Z., Rodríguez, T., Liang, S., Xue, W., & Sontheimer, E. J. (2022) · Nature Biotechnology · doi:10.1038/s41587-022-01255-9 · Cited by 169 · see citing works

Ex vivo prime editing of patient haematopoietic stem cells rescues sickle-cell disease phenotypes after engraftment in mice

Everette, K. A., Newby, G. A., Levine, R. M., Mayberry, K. D., Jang, Y., Mayuranathan, T., Nimmagadda, N., Dempsey, E., Li, Y., Bhoopalan, S. V., et al. (2023) · Nature Biomedical Engineering · doi:10.1038/s41551-023-01026-0 · Cited by 167 · see citing works

A web tool for the design of prime-editing guide RNAs

Chow, R. D.-W., Chen, J., Shen, J., & Chen, S. (2020) · Nature Biomedical Engineering · doi:10.1038/s41551-020-00622-8 · Cited by 165 · see citing works

Predicting prime editing efficiency and product purity by deep learning

Mathis, N., Allam, A., Kissling, L., Marquart, K. F., Schmidheini, L., Solari, C., Balázs, Z., Krauthammer, M. O., & Schwank, G. (2023) · Nature Biotechnology · doi:10.1038/s41587-022-01613-7 · Cited by 164 · see citing works

Saturation variant interpretation using CRISPR prime editing

Erwood, S., Bily, T. M. I., Lequyer, J., Yan, J., Gulati, N., Brewer, R. A., Zhou, L., Pelletier, L., Ivakine, E. A., & Cohn, R. D. (2022) · Nature Biotechnology · doi:10.1038/s41587-021-01201-1 · Cited by 163 · see citing works

Unbiased investigation of specificities of prime editing systems in human cells

Kim, D. Y., Moon, S. B., Ko, J., Kim, Y.-S., & Kim, D. (2020) · Nucleic Acids Research · doi:10.1093/nar/gkaa764 · Cited by 154 · see citing works

Evaluating CRISPR-based prime editing for cancer modeling and CFTR repair in organoids

Geurts, M. H., de Poel, E., Pleguezuelos-Manzano, C., Oka, R., Carrillo, L., Andersson-Rolf, A., Boretto, M., Brunsveld, J. E., van Boxtel, R., Beekman, J. M., et al. (2021) · Life Science Alliance · doi:10.26508/lsa.202000940 · Cited by 151 · see citing works

Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure

Li, X., Zhou, L., Gao, B., Li, G., Wang, X., Wang, Y., Wei, J., Han, W., Wang, Z., Li, J., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-29339-9 · Cited by 139 · see citing works

Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor

Park, S.-J., Jeong, T. Y., Shin, S. K., Yoon, D. E., Lim, S.-Y., Kim, S. P., Choi, J., Lee, H., Hong, J.-I., Ahn, J., et al. (2021) · Genome biology · doi:10.1186/s13059-021-02389-w · Cited by 129 · see citing works

Identification of herbicide resistance OsACC1 mutations via in planta prime-editing-library screening in rice

Xu, R., Liu, X., Li, J., Qin, R., & Wei, P. (2021) · Nature Plants · doi:10.1038/s41477-021-00942-w · Cited by 123 · see citing works

Prime Editing in the model plant Physcomitrium patens and its potential in the tetraploid potato

Perroud, P., Guyon‐Debast, A., Veillet, F., Kermarrec, M.-P., Chauvin, L., Chauvin, J.-É., Gallois, J., & Nogué, F. (2021) · Plant Science · doi:10.1016/j.plantsci.2021.111162 · Cited by 123 · see citing works

PE-Designer and PE-Analyzer: web-based design and analysis tools for CRISPR prime editing

Hwang, G., Jeong, Y. K., Habib, O., Hong, S.-A., Lim, K., Kim, J., & Bae, S. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkab319 · Cited by 118 · see citing works

A flexible split prime editor using truncated reverse transcriptase improves dual-AAV delivery in mouse liver

Zheng, C., Liang, S., Liu, B., Liu, P., Kwan, S.-Y., Wolfe, S. A., & Xue, W. (2022) · Molecular Therapy · doi:10.1016/j.ymthe.2022.01.005 · Cited by 117 · see citing works

Enhancement of prime editing via xrRNA motif-joined pegRNA

Zhang, G., Liu, Y., Huang, S., Qu, S., Cheng, D., Yao, Y., Ji, Q., Wang, X., Huang, X., & Liu, J. (2022) · Nature Communications · doi:10.1038/s41467-022-29507-x · Cited by 113 · see citing works

Increasing the efficiency and precision of prime editing with guide RNA pairs

Zhuang, Y., Liu, J., Wu, H., Zhu, Q., Yan, Y., Meng, H., Chen, P. R., & Yi, C. (2021) · Nature Chemical Biology · doi:10.1038/s41589-021-00889-1 · Cited by 112 · see citing works

Dual-AAV split prime editor corrects the mutation and phenotype in mice with inherited retinal degeneration

She, K., Liu, Y., Zhao, Q., Jin, X., Yang, Y., Su, J., Li, R., Song, L., Xiao, J., Yao, S., et al. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-01234-1 · Cited by 106 · see citing works

A design optimized prime editor with expanded scope and capability in plants

Xu, W., Yang, Y., Yang, B., Krueger, C. J., Xiao, Q., Zhao, S., Zhang, L., Kang, G., Wang, F., Yi, H., et al. (2021) · Nature Plants · doi:10.1038/s41477-021-01043-4 · Cited by 101 · see citing works

Engineered CRISPR prime editors with compact, untethered reverse transcriptases

Grünewald, J., Miller, B. R., Szalay, R. N., Cabeceiras, P. K., Woodilla, C. J., Holtz, E. J. B., Petri, K., & Joung, J. K. (2022) · Nature Biotechnology · doi:10.1038/s41587-022-01473-1 · Cited by 98 · see citing works

A truncated reverse transcriptase enhances prime editing by split AAV vectors

Gao, Z., Ravendran, S., Mikkelsen, N. S., Haldrup, J., Cai, H., Ding, X., Paludan, S. R., Thomsen, M. K., Mikkelsen, J. G., & Bak, R. O. (2022) · Molecular Therapy · doi:10.1016/j.ymthe.2022.07.001 · Cited by 96 · see citing works

Prime editing with genuine Cas9 nickases minimizes unwanted indels

Lee, J., Lim, K., Kim, A., Mok, Y. G., Chung, E., Cho, S.-I., Lee, J. M., & Kim, J. (2023) · Nature Communications · doi:10.1038/s41467-023-37507-8 · Cited by 96 · see citing works

Precise genome engineering in Drosophila using prime editing

Bosch, J. A., Birchak, G., & Perrimon, N. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2021996118 · Cited by 94 · see citing works

Vision rescue via unconstrained in vivo prime editing in degenerating neural retinas

Qin, H., Zhang, W., Zhang, S., Feng, Y., Xu, W., Jia, Q., Zhang, Q., Xu, C., Liu, S., Zhang, J., et al. (2023) · The Journal of Experimental Medicine · doi:10.1084/jem.20220776 · Cited by 89 · see citing works

Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants

Koeppel, J., Weller, J., Peets, E. M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante, F. G., & Parts, L. (2023) · Nature Biotechnology · doi:10.1038/s41587-023-01678-y · Cited by 88 · see citing works

Optimized nickase- and nuclease-based prime editing in human and mouse cells

Adikusuma, F., Lushington, C., Arudkumar, J., Godahewa, G. I., Chey, Y. C. J., Gierus, L., Piltz, S., Geiger, A., Jain, Y., Reti, D., et al. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkab792 · Cited by 87 · see citing works

Comprehensive analysis of prime editing outcomes in human embryonic stem cells

Habib, O., Habib, G., Hwang, G., & Bae, S. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkab1295 · Cited by 83 · see citing works

Bi-PE: bi-directional priming improves CRISPR/Cas9 prime editing in mammalian cells

Tao, R. X., Wang, Y., Jiao, Y., Hu, Y. F., Li, L., Jiang, L., Zhou, L., Qu, J., Chen, Q., & Yao, S. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkac506 · Cited by 83 · see citing works

Multiplex base- and prime-editing with drive-and-process CRISPR arrays

Yuan, Q., & Gao, X. (2022) · Nature Communications · doi:10.1038/s41467-022-30514-1 · Cited by 79 · see citing works

Genome-wide profiling of prime editor off-target sites in vitro and in vivo using PE-tag

Liang, S., Liu, P., Ponnienselvan, K., Suresh, S., Chen, Z., Kramme, C. C., Chatterjee, P., Zhu, L. J., Sontheimer, E. J., Xue, W., et al. (2023) · Nature Methods · doi:10.1038/s41592-023-01859-2 · Cited by 79 · see citing works

In vivo HSC prime editing rescues Sickle Cell Disease in a mouse model

Li, C. L., Georgakopoulou, A., Newby, G. A., Chen, P. J., Everette, K. A., Paschoudi, K., Vlachaki, E., Gil, S., Anderson, A. K., Koob, T., et al. (2023) · Blood · doi:10.1182/blood.2022018252 · Cited by 78 · see citing works

Genomic and Transcriptomic Analyses of Prime Editing Guide RNA–Independent Off-Target Effects by Prime Editors

Gao, R., Fu, Z.-C., Li, X., Wang, Y., Wei, J., Li, G., Wang, L., Wu, J., Huang, X., Yang, L. C., et al. (2022) · The CRISPR Journal · doi:10.1089/crispr.2021.0080 · Cited by 70 · see citing works

High throughput PRIME editing screens identify functional DNA variants in the human genome

Ren, X., Yang, H., Nierenberg, J. L., Sun, Y., Chen, J., Beaman, C., Pham, T., Nobuhara, M., Takagi, M. A., Narayan, V., et al. (2023) · doi:10.1101/2023.07.12.548736 · Cited by 70 · see citing works

High-throughput PRIME-editing screens identify functional DNA variants in the human genome

Ren, X., Yang, H., Nierenberg, J. L., Sun, Y., Chen, J., Beaman, C., Pham, T., Nobuhara, M., Takagi, M. A., Narayan, V., et al. (2023) · Molecular Cell · doi:10.1016/j.molcel.2023.11.021 · Cited by 70 · see citing works

Enhancing prime editing efficiency by modified pegRNA with RNA G-quadruplexes

Li, X., Wang, X., Sun, W., Huang, S., Zhong, M., Yao, Y., Ji, Q., & Huang, X. (2022) · Journal of Molecular Cell Biology · doi:10.1093/jmcb/mjac022 · Cited by 69 · see citing works

Highly efficient generation of isogenic pluripotent stem cell models using prime editing

Li, H., Busquets, O., Verma, Y., Syed, K. M., Kutnowski, N., Pangilinan, G. R., Gilbert, L. A., Bateup, H. S., Rio, D. C., Hockemeyer, D. F., et al. (2022) · eLife · doi:10.7554/elife.79208 · Cited by 67 · see citing works

Targeted genomic translocations and inversions generated using a paired prime editing strategy

Kweon, J., Hwang, H., Ryu, H., Jang, A.-H., Kim, D., & Kim, Y. (2022) · Molecular Therapy · doi:10.1016/j.ymthe.2022.09.008 · Cited by 67 · see citing works

Easy-Prime: a machine learning–based prime editor design tool

Li, Y., Chen, J., Tsai, S. Q., & Cheng, Y. (2021) · Genome biology · doi:10.1186/s13059-021-02458-0 · Cited by 66 · see citing works

Template-jumping prime editing enables large insertion and exon rewriting in vivo

Zheng, C., Liu, B., Dong, X., Gaston, N., Sontheimer, E. J., & Xue, W. (2023) · Nature Communications · doi:10.1038/s41467-023-39137-6 · Cited by 66 · see citing works

WT-PE: Prime editing with nuclease wild-type Cas9 enables versatile large-scale genome editing

Tao, R. X., Wang, Y., Hu, Y. F., Jiao, Y., Zhou, L., Jiang, L., Li, L., He, X., Li, M., Yu, Y., et al. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-00936-w · Cited by 58 · see citing works

A prime editor mouse to model a broad spectrum of somatic mutations in vivo

Ely, Z. A., Mathey‐Andrews, N., Naranjo, S., Gould, S. I., Mercer, K. L., Newby, G. A., Cabana, C. M., Rideout, W. M., Jaramillo, G. C., Khirallah, J., et al. (2023) · Nature Biotechnology · doi:10.1038/s41587-023-01783-y · Cited by 58 · see citing works

Harnessing DSB repair to promote efficient homology-dependent and -independent prime editing

Peterka, M., Akrap, N., Li, S., Wimberger, S., Hsieh, P.-P., Degtev, D., Bestas, B., Barr, J., van de Plassche, S., Mendoza-García, P., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-28771-1 · Cited by 56 · see citing works

Optimized prime editing in monocot plants using PlantPegDesigner and engineered plant prime editors (ePPEs)

Jin, S., Lin, Q., Gao, Q., & Gao, C. (2022) · Nature Protocols · doi:10.1038/s41596-022-00773-9 · Cited by 56 · see citing works

Broadening the reach and investigating the potential of prime editors through fully viral gene-deleted adenoviral vector delivery

Wang, Q., Liu, J., Janssen, J. M., Tasca, F., Mei, H., & Gonçalves, M. A. F. V. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkab938 · Cited by 46 · see citing works

Improved prime editors enable pathogenic allele correction and cancer modelling in adult micePreprint

Liu, P., Liang, S., Zheng, C., Mintzer, E., Zhao, Y. G., Ponnienselvan, K., Mir, A., Sontheimer, E. J., Gao, G., Flotte, T. R., et al. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.12.15.422970 · Cited by 42 · see citing works

Enhancing Prime Editing Efficiency and Flexibility with Tethered and Split pegRNAs

Feng, Y., Liu, S., Mo, Q., Xiao, X., Liu, P., & Ma, H. (2022) · Protein & Cell · doi:10.1093/procel/pwac014 · Cited by 42 · see citing works

Modeling a cataract disorder in mice with prime editing

Lin, J., Liu, X., Lu, Z., Huang, S., Wu, S., Yu, W., Liu, Y., Zheng, X., Huang, X., Sun, Q., et al. (2021) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2021.06.020 · Cited by 38 · see citing works

Development of a versatile nuclease prime editor with upgraded precision

Li, X., Zhang, G., Huang, S., Liu, Y., Tang, J., Zhong, M., Wang, X., Sun, W., Yao, Y., Ji, Q., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-35870-0 · Cited by 38 · see citing works

Enhancing Prime Editing Efficiency and Flexibility with Tethered and Split pegRNAsPreprint

Feng, Y., Liu, S., Mo, Q., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.04.05.487236 · Cited by 23 · see citing works

MutSα and MutSβ as size-dependent cellular determinants for prime editing in human embryonic stem cells

Park, J.-C., Kim, Y.-J., Han, J. H., Kim, D., Park, M. J., Kim, J., Jang, H., Bae, S., & Cha, H. (2023) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2023.05.015 · Cited by 17 · see citing works

Generation of sheep with defined FecBB and TBXT mutations and porcine blastocysts with KCNJ5G151R/+ mutation using prime editing

Zhou, S., Lenk, L. J., Gao, Y., Wang, Y., Zhao, X., Pan, M., Huang, S., Sun, K., Kalds, P., Luo, Q., et al. (2023) · BMC Genomics · doi:10.1186/s12864-023-09409-y · Cited by 15 · see citing works

Chromatin structure and context-dependent sequence features control prime editing efficiency

Kim, S., Yuan, J. B., Woods, W. S., Newton, D. A., Perez-Pinera, P., & Song, J. S. (2023) · Frontiers in Genetics · doi:10.3389/fgene.2023.1222112 · Cited by 12 · see citing works

Prime editor-mediated correction of a pathogenic mutation in purebred dogs

Kim, D. E., Lee, J. H., Ji, K. B., Lee, E. J., Li, C., Oh, H. J., Park, K. S., Lee, S. H., Koo, O. J., & Kim, M. K. (2022) · Scientific Reports · doi:10.1038/s41598-022-17200-4 · Cited by 6 · see citing works

Revolutionizing genetic disease treatment: Recent technological advances in base editing

Daniel, T. C., Zeng, H., Osikpa, E. C., & Gao, X. (2023) · Current Opinion in Biomedical Engineering · doi:10.1016/j.cobme.2023.100472 · Cited by 5 · see citing works

State of the art CRISPR-based strategies for cancer diagnostics and treatment

Carlo, E. D., & Sorrentino, C. (2024) · Biomarker Research · doi:10.1186/s40364-024-00701-x · Cited by 66 · see citing works

Emerging applications of gene editing technologies for the development of climate-resilient crops

Chavhan, R. L., Jaybhaye, S. G., Hinge, V. R., Deshmukh, A. S., Shaikh, U. S., Jadhav, P. K., Kadam, U. S., & Hong, J. C. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1524767 · Cited by 43 · see citing works

Overcoming the Delivery Challenges in CRISPR/Cas9 Gene Editing for Effective Cancer Treatment: A Review of Delivery Systems

Tang, S., Chen, X.-Y., Tong, X.-M., & Zhu, L.-F. (2025) · International Journal of Medical Sciences · doi:10.7150/ijms.112724 · Cited by 23 · see citing works

Evolution of agricultural biotechnology is the paradigm shift in crop resilience and development: a review

Riaz, M., Yasmeen, E., Saleem, B., Hameed, M. K., Almheiri, M. T. S., Mir, R. O. S. A., Alameri, G., Alghafri, J. S. K., & Gururani, M. A. (2025) · Frontiers in Plant Science · doi:10.3389/fpls.2025.1585826 · Cited by 28 · see citing works

From pollutants to products: Microbial cell factories driving sustainable biomanufacturing and environmental conservation

Gavrilescu, M. C. (2024) · Chemical Engineering Journal · doi:10.1016/j.cej.2024.157152 · Cited by 19 · see citing works

Prime Editing: The Next Frontier in Precision Gene Therapy

Aliciaslan, M., Erbasan, E., Erendor, F., & Şanlıoğlu, S. (2025) · The Journal of Gene Medicine · doi:10.1002/jgm.70040 · Cited by 13 · see citing works

The future of complement therapeutics

Kolev, M. V., Rao, K. N., Yeh, M. W., Parikh, A., & Deschatelets, P. (2024) · Exploration of Immunology · doi:10.37349/ei.2024.00161 · Cited by 12 · see citing works

Make-or-break prime editing for genome engineering in Streptococcus pneumoniae

Monica, R.-G., Mazzuoli, M.-V., Janssen, A. B., Rueff, A.-S., Burnier, J., Liu, X., & Veening, J. (2025) · Nature Communications · doi:10.1038/s41467-025-59068-8 · Cited by 13 · see citing works

Advances in large-scale DNA engineering with the CRISPR system

Gwon, L. W., Badon, I. W., Lee, Y., Kim, H., & Lee, S. H. (2025) · Experimental & Molecular Medicine · doi:10.1038/s12276-025-01530-0 · Cited by 12 · see citing works

CRISPR/Cas9 Genome Editing in the Diamondback Moth: Current Progress, Challenges, and Prospects

Asad, M., Chang, Y., Liao, J., & Yang, G. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26041515 · Cited by 9 · see citing works

Orthogonal and multiplexable genetic perturbations with an engineered prime editor and a diverse RNA array

Yuan, Q., Zeng, H., Daniel, T. C., Liu, Q., Yang, Y., Osikpa, E. C., Yang, Q., Peddi, A., Abramson, L. M., Zhang, B., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-55134-9 · Cited by 6 · see citing works

CRISPR-Cas editing technologies for viral-mediated gene therapies of human diseases: Mechanisms, progress, and challenges

Kantor, B., Duke, L. C., & Bhide, P. G. (2025) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2025.102786 · Cited by 6 · see citing works

Prime editing: Emerging mechanisms, engineering innovations, and next-generation applications

Muhammad, W., Amjad, A., Liu, Y., Umar, A., Chen, K., Wang, X., & Xu, K. (2026) · BioDesign Research · doi:10.1016/j.bidere.2026.100073 · Cited by 3 · see citing works

Developing CRISPR-Based Therapies for Epidermolysis Bullosa: A Comprehensive Review of Current Strategies

du Rand, A., Buttle, B., & Sheppard, H. M. (2026) · Drugs · doi:10.1007/s40265-026-02285-2 · Cited by 2 · see citing works

PrimeRoot: a cutting-edge technology designed to achieve precise and targeted large DNA insertion in plants

Negi, C., Vyas, P., Dhariwal, R., & Vasistha, N. K. (2026) · 3 Biotech · doi:10.1007/s13205-026-04723-0 · Cited by 2 · see citing works

First report of CRISPR prime editing in a globally significant non-model organism, the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae)

Dhawane, Y., Shashikala, T., Geerthana, S., Kumar, A., Kaninika, V. K., Manamohan, M., Jithesh, M. N., Jha, G. K., Asokan, R., & Ashok, K. R. (2026) · Methods · doi:10.1016/j.ymeth.2026.03.001 · Cited by 2 · see citing works

Efficient genome editing with chimeric oligonucleotide-directed editing

Nguyễn, L. T., Rakestraw, N. R., Pizzano, B. L. M., Iyyappan, R., Young, C. B., Huang, Y., Beerensson, K. T., Fang, A., Antal, S. G., Anamisis, K. V., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-71624-4 · Cited by 2 · see citing works

Bidirectional Prime Editing: Combining Precision with Versatility for Genome Editing

Choudhery, M. S., Arif, T., & Mahmood, R. (2024) · Cellular Reprogramming · doi:10.1089/cell.2024.0075 · Cited by 2 · see citing works

Make-or-break prime editing for bacterial genome engineeringPreprint

Monica, R.-G., Mazzuoli, M.-V., Janssen, A. B., Rueff, A.-S., Burnier, J., Liu, X., & Veening, J. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.06.27.601116 · Cited by 2 · see citing works

Therapeutic Advances in Major NBIA Disorders: Current Strategies and Translational Challenges

Cascone, F., Gasparini, G., Tiranti, V., & Meo, I. D. (2026) · Neurology International · doi:10.3390/neurolint18070133 · Cited by 1 · see citing works

CRISPR-Cas9 and beyond: Expanding the Frontiers of Gene Editing

Pang, S., & Qu, J. (2025) · Advances in Bioscience and Biotechnology · doi:10.4236/abb.2025.165012

Optimizing twin prime editing components for scalable genome editing and therapy in spinocerebellar ataxia type 3

Gwon, L. W., Seong, J. B., Yeo, H.-G., Oh, Y., Park, J., Won, J., Park, S. J., Kim, Y., Huh, J., Kim, A., et al. (2026) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2026.102988

Prime Editing Enables High-Efficiency Correction of the Ryr1 T4706M Mutation: A Promising Therapeutic Approach for RyR1-Related MyopathiesPreprint

Godbout, K., Rousseau, J., Canet, G., & Tremblay, J. P. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.09.12.675683

Precision prime editing of TP53 mutations for functional tumor suppression in colorectal cancer

Azhar, M., Malviya, R., Chandra, P., Sridhar, S. B., Shareef, J., & Wadhwa, T. (2026) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2026.154311

Beyond Earth: Recent Advancements in Microgravity Biomedical and Genetic Research in Saudi Arabia

Hawsawi, Y. M., Jamous, Y. F., Alghannam, S. F., Aldahshan, H., Alothman, L., Fitaihi, R. A., Aljawini, N., Alqarni, S. S., Alfaraj, R., Aldkheil, E. A., et al. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27177613

Minimal indel-inducing and window-adjustable base editing using inactive Cas12b effectors

Lee, W.-J., Kim, Y. G., Oh, Y., Gwon, L. W., Lim, D., Kim, H., Sim, B.-W., & Lee, S. H. (2026) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2026.103121

Beyond Viruses: Transposons and CRISPR-Programmable Nonviral Systems for Genome Engineering.

Singh, S., Panchal, O., & Ivics, Z. (2026) · Human Gene Therapy · doi:10.1177/10430342261493722

Prime Editing: An All-Rounder for Genome Editing

Lu, C., Kuang, J., Shao, T., Xie, S., Li, M., Zhu, L., & Zhu, L. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23179862 · Cited by 38 · see citing works

Emerging trends in prime editing for precision genome editing

Lee, J., Kweon, J., & Kim, Y. (2025) · Experimental & Molecular Medicine · doi:10.1038/s12276-025-01463-8 · Cited by 60 · see citing works

Prime editing updates: technological evolution, methodological expansion, and delivery strategies for in vivo applications

Jong, U., Lim, D., & Lee, S. H. (2026) · BMB Reports · doi:10.5483/bmbrep.2026-0047 · Cited by 1 · see citing works

Prime Editing: The Next Frontier in Precision Gene Therapy

Aliciaslan, M., Erbasan, E., Erendor, F., & Şanlıoğlu, S. (2025) · The Journal of Gene Medicine · doi:10.1002/jgm.70040 · Cited by 13 · see citing works

Prime editing: Emerging mechanisms, engineering innovations, and next-generation applications

Muhammad, W., Amjad, A., Liu, Y., Umar, A., Chen, K., Wang, X., & Xu, K. (2026) · BioDesign Research · doi:10.1016/j.bidere.2026.100073 · Cited by 3 · see citing works