Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 69

The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.

Drug Inducible CRISPR/Cas Systems

Zhang, Jingfang, Chen, Li, Zhang, Ju, Wang, Yu·2019·Computational and Structural Biotechnology Journal
DOI 10.1016/j.csbj.2019.07.015PMC PMC6709367
Open this paper in CoChat — free account

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.csbj.2019.07.015

Cites

11 of 11 references verified0 citation errors · 0 not checkable

Statistics

Figures

Graphical abstract
Graphical abstract CC BY-NC-ND · PubMed Central
Fig. 1 CRISPR/Cas9 systems of drug induction at the transcriptional level. A. Tet based CRISPR/Cas9 systems for doxycycline regulation of Cas9 (left) or sgRNA (right) expression. AD, activation domain. B. Cre-ERT2-dependent CRISPR/Cas9 system. Cre-ERT2 removes the loxp-stop-loxp cassette and drives Cas9 expression dependent on 4-OHT regulated nuclear translocation.
Fig. 1 CRISPR/Cas9 systems of drug induction at the transcriptional level. A. Tet based CRISPR/Cas9 systems for doxycycline regulation of Cas9 (left) or sgRNA (right) expression. AD, activation domain. B. Cre-ERT2-dependent CRISPR/Cas9 system. Cre-ERT2 removes the loxp-stop-loxp cassette and drives Cas9 expression dependent on 4-OHT regulated nuclear translocation.CC BY-NC-ND · PubMed Central
Fig. 2 CRISPR/Cas9 systems of drug induction at the posttranslational level. A. Chemically induced proximity (CIP) systems. Drug induces split Cas9 dimerization and forming complete and functional Cas9 (upper panel); similarly, drug induces dimerization of dCas9 and transcriptional activation domains (ADs) for gene activation (lower panel). NES, nuclear export sequence; NLS, nuclear localization sequence. B. Intein splicing CRISPR/Cas9 system. C. Systems based on 4-OHT driven ER nuclear translocation. D. Allosterically regulated Cas9. E. Destabilized domains (DDs) controlled CRISPR/Cas9 systems. Small molecule stabilizes DD-Cas9 (left) or DD-PP7-ADs (right) and renders them drug inducible. DSB, double strand break.
Fig. 2 CRISPR/Cas9 systems of drug induction at the posttranslational level. A. Chemically induced proximity (CIP) systems. Drug induces split Cas9 dimerization and forming complete and functional Cas9 (upper panel); similarly, drug induces dimerization of dCas9 and transcriptional activation domains (ADs) for gene activation (lower panel). NES, nuclear export sequence; NLS, nuclear localization sequence. B. Intein splicing CRISPR/Cas9 system. C. Systems based on 4-OHT driven ER nuclear translocation. D. Allosterically regulated Cas9. E. Destabilized domains (DDs) controlled CRISPR/Cas9 systems. Small molecule stabilizes DD-Cas9 (left) or DD-PP7-ADs (right) and renders them drug inducible. DSB, double strand break.CC BY-NC-ND · PubMed Central
Fig. 3 Schematic presentation of two orthogonal CRISPR/Cas9 systems. A. Two independent chemically induced proximity (CIP) systems in combination with spCas9 and saCas9 respectively form an orthogonal CRISPR/Cas9 system for independent regulation of two genes. KRAB, Krüppel-associated box; VPR, VP64-P65-Rta. B. Two independent destabilized domain (DD) systems in combination with distinct aptamers constitute another orthogonal system. PH, P65-HSF1.
Fig. 3 Schematic presentation of two orthogonal CRISPR/Cas9 systems. A. Two independent chemically induced proximity (CIP) systems in combination with spCas9 and saCas9 respectively form an orthogonal CRISPR/Cas9 system for independent regulation of two genes. KRAB, Krüppel-associated box; VPR, VP64-P65-Rta. B. Two independent destabilized domain (DD) systems in combination with distinct aptamers constitute another orthogonal system. PH, P65-HSF1.CC BY-NC-ND · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 11 references

Research works printed but not linked: 76

  1. 10.1016/j.cell.2014.05.010 · DOI · Paper page
  2. 10.1126/science.1138140 · DOI · Paper page
  3. 10.1126/science.1179555 · DOI
  4. 10.1038/nmeth.2600 · DOI · Paper page
  5. 10.1021/ja512664v · DOI
  6. 10.1016/j.chembiol.2014.12.011 · DOI
  7. Toth L.P. and Gersbach C.A.: Mol Ther May, 2015; 23: S275. · DOI
  8. 10.1038/nbt.3245 · DOI
  9. Zhou X.X. et al: ACS Chem Biol 2017; 13: 443. · DOI
  10. Kawano F., Suzuki H., Furuya A. and Sato M.: Nat Commun Feb, 2015; 6. · DOI
  11. 10.1073/pnas.130192197 · DOI · Paper page
  12. Cao J. et al: Nucleic Acids Res Nov 2, 2016; 44: e149.
  13. 10.1016/j.stem.2014.05.018 · DOI
  14. 10.1038/nbt.3155 · DOI
  15. 10.1038/nmeth.3312 · DOI · Paper page
  16. 10.1016/j.celrep.2015.02.002 · DOI
  17. 10.1073/pnas.0806213105 · DOI
  18. 10.1016/S1534-5807(03)00399-X · DOI
  19. 10.1016/j.cell.2014.09.014 · DOI · Paper page
  20. StOnge L., Furth P.A. and Gruss P.: Nucleic Acids Res Oct 1, 1996; 24: 3875. · DOI
  21. 10.1016/S0378-1119(99)00045-1 · DOI
  22. Feil R. et al: Proc Natl Acad Sci U S A Oct 1, 1996; 93: 10887. · DOI · Paper page
  23. Indra A.K. et al: Nucleic Acids Res Nov 15, 1999; 27: 4324. · DOI
  24. Kellendonk C. et al: Nucleic Acids Res Apr 15, 1996; 24: 1404. · DOI
  25. Wunderlich F.T., Wildner H., Rajewsky K. and Edenhofer F.: Nucleic Acids Res May 15, 2001; 29. · DOI
  26. Oldrini B. et al: Nat Commun Apr 13, 2018; 9: 1466. · DOI
  27. 10.1038/nbt.3836 · DOI
  28. 10.1016/1074-5521(94)90002-7 · DOI
  29. Rivera V.M., Berk L. and Clackson T.: Cold Spring Harbor Protoc Jul 1, 2012; 2012: 821.
  30. 10.1126/scisignal.2001449 · DOI
  31. 10.1038/nchembio.922 · DOI
  32. 10.1016/j.cbpa.2015.09.003 · DOI
  33. 10.1126/science.273.5272.239 · DOI
  34. 10.1038/nbt.3149 · DOI
  35. Nguyen D.P. et al: Nat Commun Jul 1, 2016; 7: 12009. · DOI
  36. 10.1073/pnas.1501698112 · DOI
  37. 10.1038/nmeth.4042 · DOI
  38. 10.1016/j.cell.2015.09.038 · DOI · Paper page
  39. 10.1038/nature17945 · DOI · Paper page
  40. 10.1016/j.cell.2016.04.003 · DOI
  41. Tang X. et al: Nat Plants Feb 17, 2017; 3: 17018. · DOI
  42. Zhang X. et al: Cell Discov 2017; 3: 17018. · DOI
  43. Tak Y.E. et al: Nat Methods Dec, 2017; 14: 1163. · DOI
  44. 10.1146/annurev.biochem.69.1.447 · DOI
  45. 10.1146/annurev.micro.56.012302.160741 · DOI
  46. Fine E.J. et al: Sci Rep Jul 1, 2015; 5: 10777. · DOI
  47. 10.1038/nmeth.3993 · DOI · Paper page
  48. Ma D., Peng S. and Xie Z.: Nat Commun Oct 3, 2016; 7: 13056. · DOI
  49. Zeidler M.P. et al: Nat Biotechnol Jul, 2004; 22: 871. · DOI
  50. Ren W., Ji A. and Ai H.W.: J Am Chem Soc Feb 18, 2015; 137: 2155. · DOI
  51. 10.1110/ps.04996905 · DOI
  52. 10.1073/pnas.0402762101 · DOI
  53. 10.1016/j.chembiol.2011.02.014 · DOI
  54. 10.1038/nchembio.1793 · DOI
  55. Danielian P.S., White R., Hoare S.A., Fawell S.E. and Parker M.G.: Mol Endocrinol Feb, 1993; 7: 232.
  56. 10.1006/bbrc.1997.7124 · DOI
  57. Danielian P.S., Muccino D., Rowitch D.H., Michael S.K. and McMahon A.P.: Curr Biol Dec 3, 1998; 8: 1323. · DOI
  58. 10.1093/nar/23.10.1686 · DOI
  59. 10.1093/nar/26.6.1427 · DOI
  60. 10.1016/j.omtn.2018.08.022 · DOI
  61. 10.1038/nchembio.2179 · DOI
  62. 10.1093/nar/gkx1222 · DOI
  63. 10.1016/j.cell.2015.08.007 · DOI
  64. 10.1038/nbt.3528 · DOI
  65. 10.1016/j.cell.2006.07.025 · DOI · Paper page
  66. 10.1038/nm.1754 · DOI
  67. 10.1016/j.chembiol.2010.07.009 · DOI
  68. 10.1038/nmeth.2640 · DOI
  69. 10.1073/pnas.1018449108 · DOI
  70. 10.1038/mt.2013.169 · DOI
  71. 10.1016/j.stemcr.2015.08.001 · DOI
  72. 10.1021/ja209933r · DOI
  73. 10.1038/nchembio.2224 · DOI
  74. Senturk S. et al: Nat Commun Feb 22, 2017; 8: 1. · DOI
  75. 10.1016/j.cell.2014.11.052 · DOI · Paper page
  76. 10.1021/acschembio.7b00606 · DOI

Cited by

See all 69

Metadata

Authors
Zhang, J., Chen, L., Zhang, J., & Wang, Y.
Affiliations
  • School of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, China
  • State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
Venue
Computational and Structural Biotechnology Journal
Publisher
American Association for the Advancement of Science (AAAS)
Published
2019-01
Volume
17
Pages
1171-1177
ISSN
2001-0370
DOI
10.1016/j.csbj.2019.07.015
PMC
PMC6709367
Cited by
69
References
11
Language
en
Licence
Funders
  • National Basic Research Program of China (2015CB964800)
  • National Natural Science Foundation of China (31701280, 31571514)
  • Chinese Academy of Sciences (XDA16010504)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Drug Inducible CRISPR/Cas Systems. https://app.cochat.ai/papers/10.1016/j.csbj.2019.07.015
The paper
Zhang, J., Chen, L., Zhang, J., & Wang, Y. (2019). Drug Inducible CRISPR/Cas Systems. Computational and Structural Biotechnology Journal. https://doi.org/10.1016/j.csbj.2019.07.015