Topical application of substance P promotes wound healing in streptozotocin-induced diabetic rats
Abstract
Substance P (SP) is known to stimulate angiogenesis, fibroblasts proliferation and expressions of cytokines and growth factors involved in wound healing. However, SP level reduces in dermis in diabetics and, hence, it was hypothesized that exogenously applied SP could be helpful in improving wound healing in diabetic rats. Excision skin wound was created on the back of diabetic rats and rats were divided into three groups i.e. (i) saline-, (ii) gel- and (iii) SP-treated. Normal saline, pluronic gel and SP (10(-6)M) in gel were topically applied once daily for 19days. SP treatment significantly increased the wound closure, levels of interleukin-10, and expressions of vascular endothelial growth factor, transforming growth factor-beta1, heme oxygenase-1 and endothelial nitric oxide synthase, whereas it significantly decreased the expression of tumor necrosis factor-alpha, interleukin-1beta and matrix metalloproteinases-9 in the granulation/healing tissue. The inflammatory cells were present for long time in normal saline-treated group. Histological evaluation revealed better extracellular matrix formation with marked fibroblast proliferation and collagen deposition in SP-treated group. Early epithelial layer formation, increased microvessel density and greater growth associated protein-43 positive nerve fibers were also evidenced in SP-treated group. In conclusion, SP treatment markedly accelerated cutaneous wound healing in diabetic rats.
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SP accelerates normal acute and chronic wound healing processes.
“SP accelerates the normal acute and chronic wound healing processes54–56.”
From Neuropeptides Substance P and Calcitonin Gene Related Peptide Accelerate the Development and Fibrogenesis of Endometriosis (2019) · cites this paper as [54]
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- Authors
- Kant, V., Kumar, D., Kumar, D., Prasad, R., Gopal, A., Pathak, N. N., Kumar, P., & Tandan, S. K.
- Affiliations
- Indian Veterinary Research Institute (IN)
- Corresponding author
- Kant, Vinay
- Venue
- Cytokine
- Publisher
- Elsevier BV
- Published
- 2015-05
- Volume
- 73
- Issue
- 1
- Pages
- 144-155
- ISSN
- 1043-4666 (ISSN-L)
- OpenAlex
- W2064688968
- Cited by
- 79
- References
- 65
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 7 · 2023: 6 · 2022: 13 · 2021: 10 · 2020: 8 · 2019: 7 · 2018: 9 · 2017: 7 · 2016: 4 · 2015: 1
- FWCI
- 7.08
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cytokine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetic Foot Ulcer Assessment and Management
- Health Sciences › Medicine › Dermatology › Skin Diseases and Diabetes
- Keywords
- substance P, diabetic wound healing, topical application, excision wound, angiogenesis, IL-10, vascular endothelial growth factor, TGF-β1, heme oxygenase-1, endothelial nitric oxide synthase, TNF-α, IL-1β, MMP-9, fibroblast proliferation, collagen deposition, extracellular matrix, microvessel density, nerve fibers, growth-associated protein 43, diabetic rats
- MeSH terms
- Administration, Topical, Animals, Axons, Blood Glucose, Collagen, Diabetes Mellitus, Experimental, Gene Expression Regulation, Male, Nerve Fibers, Nerve Regeneration, RNA, Messenger, Streptozocin, Substance P, Tumor Necrosis Factor-alpha, Wound Healing, Interleukin-10, Rats, Wistar, Neovascularization, Physiologic, Platelet Endothelial Cell Adhesion Molecule-1, GAP-43 Protein, Matrix Metalloproteinase 9, Vascular Endothelial Growth Factor A, Heme Oxygenase-1, Nitric Oxide Synthase Type III, Interleukin-1beta, Transforming Growth Factor beta1
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