Cited by 433D Printing in Biomedical ResearchCellular Mechanics and InteractionsCancer Cells and Metastasis

Biomarkers for simplifying HTS 3D cell culture platforms for drug discovery: the case for cytokines

Lai, Yinzhi, Asthana, Amish, Kisaalita, William S.·2011·Drug Discovery Today

Abstract

In this review, we discuss the microenvironmental cues that modulate the status of cells to yield physiologically more relevant three-dimensional (3D) cell-based high throughput drug screening (HTS) platforms for drug discovery. Evidence is provided to support the view that simplifying 3D cell culture platforms for HTS applications calls for identifying and validating ubiquitous three-dimensionality biomarkers. Published results from avascular tumorigenesis and early stages of inflammatory wound healing, where cells transition from a two-dimensional (2D) to 3D microenvironment, conclusively report regulation by cytokines, providing the physiological basis for focusing on cytokines as potential three-dimensionality biomarkers. We discuss additional support for cytokines that comes from numerous 2D and 3D comparative transcriptomic and proteomic studies, which generally report upregulation of cytokines in 3D compared with 2D culture counterparts.

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Crossref · 10.1016/j.drudis.2011.01.009

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  • Others seek to develop a reproducible 3D cell culture platform for high throughput screening through the identification and validation of ubiquitous three-dimensionality biomarkers, such as the cytokine family.

    “Others seek simply to develop a reproducible 3D cell culture platform for high throughput screening through the identification and validation of ubiquitous three-dimensionality biomarkers, such as the cytokine family. 72”

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Authors
Lai, Y., Asthana, A., & Kisaalita, W. S.
Affiliations
University of Georgia (US)
Corresponding author
Kisaalita, William S.
Venue
Drug Discovery Today
Publisher
Elsevier BV
Published
2011-04
Volume
16
Issue
7-8
Pages
293-297
ISSN
1359-6446 (ISSN-L)
OpenAlex
W2034085946
Cited by
43
References
34
Citations by year
2026: 2 · 2025: 3 · 2023: 1 · 2022: 4 · 2021: 1 · 2020: 3 · 2019: 3 · 2018: 3 · 2017: 5 · 2016: 2 · 2015: 2 · 2014: 3 · 2013: 5 · 2012: 5
FWCI
0.36
Type
journal article
Language
en
Open access
closed
Locations
  • Drug Discovery Today (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
Keywords
3D cell culture, high-throughput screening, cytokines, tumor microenvironment, wound healing, transcriptomics, proteomics
MeSH terms
Drug Evaluation, Preclinical, Humans, Biomarkers, Cytokines, Cell Culture Techniques, Drug Discovery, High-Throughput Screening Assays

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